The therapeutic and diagnostic role of exosomes in cardiovascular diseases

Trends in Cardiovascular Medicine, Journal Year: 2018, Volume and Issue: 29(6), P. 313 - 323

Published: Oct. 24, 2018

Language: Английский

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miR-223: An Effective Regulator of Immune Cell Differentiation and Inflammation

International Journal of Biological Sciences, Journal Year: 2021, Volume and Issue: 17(9), P. 2308 - 2322

Published: Jan. 1, 2021

MicroRNAs (miRNAs) play a critical role in regulating various biological processes, such as cell differentiation and immune modulation by binding to their target genes. miR-223 is miRNA with important functions has been widely investigated recent years. Under certain physiological conditions, regulated different transcription factors, including sirtuin1 (Sirt1), PU.1 Mef2c, its are mediated through changes cellular or tissue expression. This review paper summarizes biosynthesis regulatory the of granulocytes, dendritic cells (DCs) lymphocytes, macrophage polarization, endothelial epithelial inflammation. In addition, it describes molecular mechanisms lung inflammation, rheumatoid arthritis, enteritis, neuroinflammation mastitis provide insights into existing networks therapies for inflammatory diseases humans animals.

Language: Английский

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Long non-coding RNA MEG3 promotes fibrosis and inflammatory response in diabetic nephropathy via miR-181a/Egr-1/TLR4 axis

Aging, Journal Year: 2019, Volume and Issue: 11(11), P. 3716 - 3730

Published: June 13, 2019

Long non-coding RNAs (lncRNAs) play vital roles in diabetic nephropathy (DN). This research aimed to study the potential role and underlying molecular mechanisms of long RNA MEG3 DN.We found that was upregulated DN vivo vitro could enhance cell fibrosis inflammatory response DN.MEG3 functioned as an endogenous sponge for miR-181a mesangial cells (MCs) via direct targeting Ago2-dependent manner.MiR-181a inhibition promoted MC response.In addition, Egr-1 confirmed a target gene miR-181a.Further investigations verified promotes miR-181a/Egr-1/TLR4 axis vivo.These results provide new insights into regulation between signaling pathway during progression.

Language: Английский

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EIF4A3-regulated circ_0087429 can reverse EMT and inhibit the progression of cervical cancer via miR-5003-3p-dependent upregulation of OGN expression

Journal of Experimental & Clinical Cancer Research, Journal Year: 2022, Volume and Issue: 41(1)

Published: May 5, 2022

Circular RNAs (circRNAs) are noncoding with stable structures high expression and tissue-specific expression. Studies have shown that circRNA dysregulation is closely related to the progression of tumours. However, function regulatory mechanism most circRNAs in cervical cancer still unclear. METHODS: CircRNAs were screened through Gene Expression Omnibus (GEO) database. qRT-PCR was used verify circ_0087429 tissues cells. Then, vivo vitro experiments conducted evaluate role cancer. The circ_0087429/miR-5003-3p/osteoglycin (OGN) axis epithelial mesenchymal transition (EMT) confirmed by rescue experiments, fluorescence situ hybridization, luciferase reporter assays, immunofluorescence staining western blotting. inhibitory effect Eukaryotic initiation factor 4A-III (EIF4A3) on biogenesis verified RNA immunoprecipitation (RIP) assays qRT-PCR.circ_0087429 significantly downregulated cells negatively correlated International Federation Gynecology Obstetrics (FIGO) staging lymphatic metastasis patients. can inhibit proliferation, migration, invasion angiogenesis tumour growth vivo. OGN upregulate competitively binding miR-5003-3p, thereby reversing EMT inhibiting EIF4A3 its flanking regions.As a suppressor, regulated reverse miR-5003-3p/OGN axis. It expected become potential target for treatment

Language: Английский

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Statins Use in Alzheimer Disease: Bane or Boon from Frantic Search and Narrative Review

Brain Sciences, Journal Year: 2022, Volume and Issue: 12(10), P. 1290 - 1290

Published: Sept. 24, 2022

Alzheimer's disease (AD) was used to describe pre-senile dementia differentiate it from senile dementia, which develops in the adult age group of more than 65 years. AD is characterized by deposition amyloid beta (Aβ) plaque and tau-neurofibrillary tangles (TNTs) brain. The neuropathological changes are related plaques, neurofibrillary tangles, progression neuroinflammation, neuronal mitochondrial dysfunction, autophagy cholinergic synaptic dysfunction. Statins one main cornerstone drugs for management cardiovascular disorders regardless dyslipidemia status. Increasing use statins, mainly elderly groups primary secondary prevention diseases, may affect their cognitive functions. Extensive prolonged statins functions healthy subjects patients. Statins-induced impairments both patients health providers had been reported according post-marketing survey. This survey depends on sporadic cases, no measures were used. Evidence prospective observational studies gives robust conclusion regarding beneficial or detrimental effects Therefore, this study a narrative review aimed with evidences beneficial, detrimental, neutral AD.

Language: Английский

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Effect of Statin Therapy on Arterial Wall Inflammation Based on 18F-FDG PET/CT: A Systematic Review and Meta-Analysis of Interventional Studies

Journal of Clinical Medicine, Journal Year: 2019, Volume and Issue: 8(1), P. 118 - 118

Published: Jan. 18, 2019

Aim. To evaluate by meta-analysis of interventional studies the effect statin therapy on arterial wall inflammation. Background. Arterial exposure to low-density lipoprotein (LDL) cholesterol levels is responsible for initiation and progression atherosclerosis 18F-fluorodeoxyglucose Positron Emission Tomography-Computed Tomography (18F-FDG PET/CT) has been used detect inflammation monitor vascular anti-inflammatory effects lipid-lowering therapy. Despite a number statin-based exploring 18F-FDG uptake, these trials have produced inconsistent results. Methods. Trials with at least one treatment arm were searched in PubMed-Medline, SCOPUS, ISI Web Knowledge, Google Scholar databases. Target-to-background ratio (TBR), an indicator blood-corrected was as target variable activity. Evaluation biases, random-effects model generic inverse variance weighting, sensitivity analysis performed qualitative quantitative data assessment synthesis. Subgroup meta-regression analyses also performed. Results. Meta-analysis seven eligible studies, comprising 10 arms 287 subjects showed significant reduction TBR following (Weighted Mean Difference (WMD): −0.104, p = 0.002), which consistent both high-intensity (WMD: −0.132, 0.019) low-to-moderate intensity −0.069, 0.037). Statin dose/duration, plasma C-reactive protein level changes, baseline did not affect response statins. Conclusions. Statins effective reducing inflammation, assessed PET/CT imaging. Larger clinical should clarify whether either cholesterol-lowering or other pleiotropic mechanisms this effect.

Language: Английский

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Prognostic Role of miR-221 and miR-222 Expression in Cancer Patients: A Systematic Review and Meta-Analysis

Cancers, Journal Year: 2019, Volume and Issue: 11(7), P. 970 - 970

Published: July 11, 2019

Background: A wealth of evidence has shown that microRNAs (miRNAs) can modulate specific genes, increasing our knowledge on the fine-tuning regulation protein expression. miR-221 and miR-222 have been frequently identified as deregulated across different cancer types; however, their prognostic significance in remains controversial. In view these considerations, we performed an updated systematic review meta-analysis published data investigating effects miR-221/222 overall survival (OS) other secondary outcomes among patients. search PubMed, Web Knowledge, Cochrane Library databases was performed. Hazard ratios (HRs) 95% confidence intervals (95% CIs) were used to assess strength association. Results: Fifty studies, analyzing 6086 patients, included review. Twenty-five studies for 17 which assessed OS meta-analysis. High expression significantly predicted poor (HR: 1.48, CI: 1.14–1.93, p = 0.003 HR: 1.90, 1.43–2.54, < 0.001, respectively). Subgroup analysis revealed finding not robust one miR-222. Furthermore, high also associated with worse progression-free disease-free pooled recurrence-free survival. Conclusions: The demonstrated is prognosis whereas unclear. More work required fully elucidate role prognosis, particularly limitations existing results, including significant heterogeneity limited number some cancers.

Language: Английский

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Melatonin ameliorates aortic valve calcification via the regulation of circular RNA CircRIC3/miR‐204‐5p/DPP4 signaling in valvular interstitial cells

Journal of Pineal Research, Journal Year: 2020, Volume and Issue: 69(2)

Published: May 5, 2020

Calcific aortic valve disease (CAVD) is highly prevalent with marked morbidity and mortality rates a lack of pharmaceutical treatment options because its mechanisms are unknown. Melatonin reported to exert atheroprotective effects. However, whether melatonin protects against calcification, whose pathogenesis shares many similarities that atherosclerosis, the underlying molecular remain In this study, we found intragastric administration for 24 weeks markedly ameliorated calcification in high cholesterol diet (HCD)-treated ApoE-/- mice, as evidenced by reduced thickness calcium deposition leaflets, improved echocardiographic parameters (decreased transvalvular peak jet velocity increased area), decreased osteogenic differentiation marker (Runx2, osteocalcin, osterix) expression valves. Consistent these vivo data, also confirmed suppression vitro hVICs. Mechanistically, level CircRIC3, procalcification circular RNA, which functions acting miR-204-5p sponge positively regulate gene dipeptidyl peptidase-4 (DPP4). Furthermore, CircRIC3 overexpression abolished inhibitory effects on hVIC differentiation. Taken together, our results suggest ameliorates via regulation CircRIC3/miR-204-5p/DPP4 signaling hVICs; therefore, medication might be considered novel strategy CAVD treatment.

Language: Английский

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The pharmaco-epigenetics of hypertension: a focus on microRNA

Molecular and Cellular Biochemistry, Journal Year: 2024, Volume and Issue: 479(12), P. 3255 - 3271

Published: Feb. 29, 2024

Hypertension is a major harbinger of cardiovascular morbidity and mortality. It predisposes to higher rates myocardial infarction, chronic kidney failure, stroke, heart failure than most other risk factors. By 2025, the prevalence hypertension projected reach 1.5 billion people. The pathophysiology this disease multifaceted, as it involves nitric oxide endothelin dysregulation, reactive oxygen species, vascular smooth muscle proliferation, vessel wall calcification, among others. With advent new biomolecular techniques, various studies have elucidated gaping hole in etiology mechanisms hypertension. Indeed, epigenetics, DNA methylation, histone modification, microRNA-mediated translational silencing appear play crucial roles altering molecular phenotype into hypertensive profile. Here, we critically review experimentally determined associations between microRNA (miRNA) molecules pharmacotherapy. Particular attention given epigenetic underlying physiological responses antihypertensive drugs like candesartan, relevant clopidogrel, aspirin, statins Furthermore, how miRNA affects pharmaco-epigenetics especially highlighted.

Language: Английский

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Simvastatin induces breast cancer cell death through oxidative stress up-regulating miR-140-5p

Aging, Journal Year: 2019, Volume and Issue: 11(10), P. 3198 - 3219

Published: May 28, 2019

Statins, a class of hyperlipidemic drugs, are widely used cholesterol lowering drugs that selectively inhibit 3hydroxy-3-methylglutaryl CoA reductase, which is the rate-limiting enzyme in biosynthesis, leading to decreasing biosynthesis.Statins exert anti-tumoral effects on various cancer, including breast cancer.However, molecular mechanisms for actions were not fully elucidated.The purpose this study was elucidate statins proliferation and apoptosis ER-negative cancer cell line MDA-MB-231.Our results showed simvastatin increased expression miR-140-5p dose dependent manner via activating transcription factor NRF1, reduced induced apoptosis, we also found SLC2A1 new target miR-140-5p.In conclusion, data shed light potential presents highly promising therapeutic option, using drug miRNA combined treating cancers.

Language: Английский

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