Fast and sensitive near-infrared ratiometric fluorescent probe with a self-immolative spacer for imaging of endogenous alkaline phosphatase activity in cells and in vivo DOI
Lin Wang, Shijun Chen,

Xiaodong Ma

et al.

Talanta, Journal Year: 2022, Volume and Issue: 249, P. 123658 - 123658

Published: June 9, 2022

Language: Английский

Eu-MOF based fluorescence probe for ratiometric and visualization detection of Cu2+ DOI
Zhihua Li, Lin Chen, Jiaxi Deng

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2023, Volume and Issue: 304, P. 123367 - 123367

Published: Sept. 6, 2023

Language: Английский

Citations

16
Rational Design of Organelle-Targeted Fluorescent Probes: Insights from Artificial Intelligence DOI Creative Commons
Jie Dong, Jie Qian,

Kunqian Yu

et al.

Research, Journal Year: 2023, Volume and Issue: 6

Published: Jan. 1, 2023

Monitoring the physiological changes of organelles is essential for understanding local biological information cells and improving diagnosis therapy diseases. Currently, fluorescent probes are considered as most powerful tools imaging have been widely applied in biomedical fields. However, expected targeting effects these often inconsistent with real experiments. The design mainly depends on empirical knowledge researchers, which was inhibited by limited chemical space low efficiency. Herein, we proposed a novel multilevel framework prediction organelle-targeted employing advanced artificial intelligence algorithms. In this way, not only mechanism could be interpreted beyond intuitions but also quick evaluation method established rational design. Furthermore, powers optimized synthesized based methodology were verified quantitative calculation

Language: Английский

Citations

14
The development of biosensors for alkaline phosphatase activity detection based on a phosphorylated DNA probe DOI
Yudong Wu,

Rongxiang Liang,

Wujun Chen

et al.

Talanta, Journal Year: 2024, Volume and Issue: 270, P. 125622 - 125622

Published: Jan. 11, 2024

Language: Английский

Citations

5
Biosensors; a novel concept in real-time detection of autophagy DOI
Hassan Nasrollahpour,

Arezoo Mirzaie,

Maryam Sharifi

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 254, P. 116204 - 116204

Published: March 16, 2024

Language: Английский

Citations

5
Alkaline Phosphatase-Triggered Etching of Au@FeOOH Nanoparticles for Enzyme Level Assay under Dark-Field Microscopy DOI
Hui Wang, Conghui Xu, Wei Zhao

et al.

Analytical Chemistry, Journal Year: 2021, Volume and Issue: 93(30), P. 10727 - 10734

Published: July 23, 2021

In clinical diagnosis, the level of biological enzymes in serum has been generally regarded as markers human diseases. this work, a kind simple and sensitive plasmonic probe (indicated Au@FeOOH) synthesized with guidance imaging subsequently developed for alkaline phosphatase (ALP) detection under dark-field microscopy (DFM). As hydrolysis enzyme, ALP can promote l-ascorbic acid 2-phosphate to ascorbic (AA). AA further acts strong reduction reagent decomposition FeOOH shell, which results blue shift localized surface plasmon resonance spectra an obvious color change DFM. RGB analyses show that using ΔR/G value instead scattering wavelength or R/G analytical signal, deviation attributed size distribution initial Au NPs is greatly suppressed, linear range from 0.2 6.0 U/L (R2 = 0.99) limit 0.06 are acquired various concentrations during detection. Besides, approach exhibits excellent selectivity complex samples, expected be applied early diagnosis diseases by monitoring biomarkers future.

Language: Английский

Citations

30
A phenothiazine coumarin based ratiometric fluorescent probe for real-time detection of lysosomal hypochlorite in living cell and zebra fish DOI
Jiaojiao Liu, Peixin Niu, Yifan Rong

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2021, Volume and Issue: 261, P. 120024 - 120024

Published: May 26, 2021

Language: Английский

Citations

28
Responsive photonic alginate hydrogel particles for the quantitative detection of alkaline phosphatase DOI Creative Commons
Yuetong Wang, Dagan Zhang, Hui Zhang

et al.

NPG Asia Materials, Journal Year: 2022, Volume and Issue: 14(1)

Published: June 24, 2022

Abstract Alkaline phosphatase (ALP) is an important marker for many diseases, yet efficient and reliable detection method still lacking. Here, we present a novel photonic alginate hydrogel particle as ALP sensor based on the competitive combination of intermediary agent, pyrophosphate ion (PPi), with crosslinker, which can trigger phase-transition response from hydrogel. This be converted into dual optical readouts, fluorescence signal structural color signal. Quantitative determination was established this dual-indicator system high accuracy reliability. These features suggest potential ideal applications responsive particles to detect other macromolecules.

Language: Английский

Citations

21
Fluorescent filter paper with pH-responsive carbon dots for the on-site detection of biogenic amines in food DOI

Jianfeng Yan,

Quanbin Fu,

Shikai Zhang

et al.

New Journal of Chemistry, Journal Year: 2023, Volume and Issue: 47(16), P. 7588 - 7594

Published: Jan. 1, 2023

A ratiometric fluorescent sensor for eight kinds of biogenic amines was established by facile mixing pH-responsive carbon dots and Rhodamine B.

Language: Английский

Citations

12
A fluorescent optical fiber sensor for real-time, portable detection of alkaline phosphatase activity DOI
Dan Li, Yi Shen, Na Li

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137568 - 137568

Published: March 1, 2025

Language: Английский

Citations

0
Constructing a novel fluorescence detection method for γ-glutamyltranspeptidase and application on visualizing liver injury DOI
Kai Wang, Wei Wang, Xu-Yang Chen

et al.

Biosensors and Bioelectronics, Journal Year: 2022, Volume and Issue: 219, P. 114767 - 114767

Published: Sept. 30, 2022

Language: Английский

Citations

17