Electrochemical paper-based antigen sensing platform using plant-derived monoclonal antibody for detecting SARS-CoV-2

Talanta, Journal Year: 2022, Volume and Issue: 251, P. 123783 - 123783

Published: Aug. 7, 2022

Language: Английский

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Microfluidic Approaches for Affinity-Based Exosome Separation

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(16), P. 9004 - 9004

Published: Aug. 12, 2022

As a subspecies of extracellular vesicles (EVs), exosomes have provided promising results in diagnostic and theranostic applications recent years. The nanometer-sized can be extracted by liquid biopsy from almost all body fluids, making them especially suitable for mainly non-invasive point-of-care (POC) applications. To achieve this, must first separated the respective biofluid. Impurities with similar properties, heterogeneity exosome characteristics, time-related biofouling complicate separation. This practical review presents state-of-the-art methods available separation exosomes. Furthermore, it is shown how new developed. A particular focus lies on fabrication design microfluidic devices using highly selective affinity Due to their compactness, quick analysis time portable form factor, these are particularly deliver fast reliable POC For devices, manufacturing (e.g., laminating, replica molding 3D printing) that use low-cost materials do not require clean rooms presented. Additionally, special flow routes patterns increase contact surfaces, as well residence time, thus improve purification displayed. Finally, various analyses used evaluate newly developed device. Overall, this paper provides toolbox developing

Language: Английский

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Nanotechnology-Assisted Biosensors for the Detection of Viral Nucleic Acids: An Overview

Biosensors, Journal Year: 2023, Volume and Issue: 13(2), P. 208 - 208

Published: Jan. 30, 2023

The accurate and rapid diagnosis of viral diseases has garnered increasing attention in the field biosensors. development highly sensitive, selective, accessible biosensors is crucial for early disease detection preventing mortality. However, developing optimized several limitations, including mutations. For decades, nanotechnology been applied numerous biological fields such as biosensors, bioelectronics, regenerative medicine. Nanotechnology offers a promising strategy to address current limitations conventional nucleic acid-based implementation nanotechnologies, functional nanomaterials, nanoplatform-fabrication techniques, surface nanoengineering, not only improved performance but also expanded range sensing targets. Therefore, deep understanding combination nanotechnologies required prepare sanitary emergencies recent COVID-19 pandemic. In this review, we provide interdisciplinary information on nanotechnology-assisted First, representative are discussed, after which review summarizes various acid expect that will valuable basis novel

Language: Английский

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Point-of-Care Devices for Viral Detection: COVID-19 Pandemic and Beyond

Micromachines, Journal Year: 2023, Volume and Issue: 14(9), P. 1744 - 1744

Published: Sept. 7, 2023

The pandemic of COVID-19 and its widespread transmission have made us realize the importance early, quick diagnostic tests for facilitating effective cure management. primary obstacles encountered were accurately distinguishing from other illnesses including flu, common cold, etc. While polymerase chain reaction technique is a robust determination SARS-CoV-2 in patients COVID-19, there arises high demand affordable, quick, user-friendly, precise point-of-care (POC) therapeutic settings. necessity available with rapid outcomes spurred advancement POC that are characterized by speed, automation, precision accuracy. Paper-based devices gained increasing interest recent years because rapid, low-cost detection without requiring external instruments. At present, microfluidic paper-based analysis garnered public attention accelerated development such POCT efficient multistep assays. In current review, our focus will be on fabrication modules SARS-CoV-2. Here, we included discussion various strategies viral moieties. compilation these would offer comprehensive insight into causative agent preparedness future pandemics. We also provide descriptive outline platforms, involving mechanisms, as well commercial kit their outlook.

Language: Английский

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Recent Advances In the development of enzymatic paper-based microfluidic biosensors

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 226, P. 115131 - 115131

Published: Feb. 6, 2023

Language: Английский

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Engineering carbon nanotubes for sensitive viral detection

TrAC Trends in Analytical Chemistry, Journal Year: 2022, Volume and Issue: 153, P. 116659 - 116659

Published: April 30, 2022

Language: Английский

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The use of biological fluids in microfluidic paper-based analytical devices (μPADs): Recent advances, challenges and future perspectives

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 246, P. 115846 - 115846

Published: Nov. 20, 2023

Language: Английский

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Paper-based biosensors based on multiple recognition modes for visual detection of microbially contaminated food

Journal of Future Foods, Journal Year: 2023, Volume and Issue: 4(1), P. 61 - 70

Published: June 7, 2023

Microbially contaminated food can cause serious health hazards and economic losses, therefore sensitive, rapid, highly specific visual detection is called for. Traditional of microorganisms complex time-consuming, which cannot meet current testing demands. The emergence paper-based biosensors provided an effective method for efficient microorganisms, due to its high speed, all-in-one device, low cost, convenience. This review focused on 5 biomarkers, namely nucleic acids, proteins, lipopolysaccharides, metabolites, the whole microorganism microorganisms. Besides, recognition methods were summed up in forms, including immunological recognition, aptamer acid amplification-mediated DNAzyme clustered regularly interspaced short palindromic repeats mediated recognition. In addition, we summarized applications thoroughly. Through exploration different identification methods, applications, hope provide a reference development their application safeguarding chain.

Language: Английский

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Wheat Germ Agglutinin‐Modified “Three‐in‐One” Multifunctional Probe Driven Broad‐Spectrum and Flexible Immunochromatographic Diagnosis of viruses With High Sensitivity

Small, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 22, 2024

Abstract The conventional lateral flow assay (LFA) fails to the demands for accurate screening of viruses as a result its low sensitivity colorimetric signal output and poor universality limited by antibody pairs. Here, magnetically assisted dual‐signal LFA platform is developed ultrasensitive, universal, flexible detection viruses. A “three‐in‐one” multifunctional probe (MAuDQD) prepared using 180 nm Fe 3 O 4 core load numerous Au nanoparticles (NPs) two layers QDs, which can substantially improve through coupling with effects magnetic enrichment colorimetric/fluorescent enhancement. Wheat germ agglutinin (WGA)‐modified MAuDQD attained broad‐spectrum capture viral membrane proteins dual‐mode severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) monkeypox virus (MPXV) on strip. In mode, target detected directly, visual reaching 0.1–0.5 ng mL −1 fluorescent mode supported quantitative analysis SARS‐CoV‐2/MPXV limits decreasing pg level. Practicability MAuDQD@WGA‐LFA verified 33 real clinical samples, showing proposed has great potential become sensitive, accurate, universal tool on‐site monitoring infections.

Language: Английский

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Enhancement of the Detection Performance of Paper-Based Analytical Devices by Nanomaterials

Molecules, Journal Year: 2022, Volume and Issue: 27(2), P. 508 - 508

Published: Jan. 14, 2022

Paper-based analytical devices (PADs), including lateral flow assays (LFAs), dipstick and microfluidic PADs (μPADs), have a great impact on the healthcare realm environmental monitoring. This is especially evident in developing countries because PADs-based point-of-care testing (POCT) enables to rapidly determine various (bio)chemical analytes miniaturized, cost-effective user-friendly manner. Low sensitivity poor specificity are main bottlenecks associated with PADs, which limit entry of into real-life applications. The application nanomaterials showing improvement their detection performance terms sensitivity, selectivity accuracy since unique physicochemical properties. In this review, research progress nanomaterial-based summarized by highlighting representative recent publications. We mainly focus principles, sensing mechanisms how they work applications disease diagnosis, monitoring food safety management. addition, limitations challenges development discussed, further directions field proposed.

Language: Английский

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