Advanced Materials,
Journal Year:
2023,
Volume and Issue:
36(22)
Published: Nov. 28, 2023
Abstract
Extracellular
vesicles
(EVs)
are
cell‐derived
nanovesicles
comprising
a
myriad
of
molecular
cargo
such
as
proteins
and
nucleic
acids,
playing
essential
roles
in
intercellular
communication
physiological
pathological
processes.
EVs
have
received
substantial
attention
noninvasive
biomarkers
for
disease
diagnosis
prognosis.
Owing
to
their
ability
recognize
protein
acid
targets,
DNA‐based
nanomaterials
with
excellent
programmability
modifiability
provide
promising
tool
the
sensitive
accurate
detection
carried
by
EVs.
In
this
perspective,
recent
advancements
EV
analysis
using
variety
summarized,
which
can
be
broadly
classified
into
three
categories:
linear
DNA
probes,
nanostructures,
hybrid
nanomaterials.
The
design,
construction,
advantages,
disadvantages
different
types
nanomaterials,
well
performance
detecting
reviewed.
challenges
opportunities
field
also
discussed.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(19), P. 7780 - 7786
Published: May 2, 2024
Development
of
highly
efficient,
heavy-metal-free
electrochemiluminescence
(ECL)
materials
is
attractive
but
still
challenging.
Herein,
we
report
an
aggregation-induced
delayed
ECL
(AIDECL)
active
organic
dot
(OD)
composed
a
tert-butoxy-group-substituted
benzophenone-dimethylacridine
compound,
which
shows
high
efficiency.
The
resultant
ODs
exhibit
2.1-fold
higher
efficiency
compared
to
control
AIDECL-active
ODs.
Molecular
stacking
combined
with
theoretical
calculations
suggests
that
tert-butoxy
groups
effectively
participate
in
the
intermolecular
interactions,
further
inhibiting
molecular
motions
aggregated
states
and
thus
accelerating
radiative
decay.
On
basis
these
exhibiting
excellent
performance,
proof-of-concept
biosensor
constructed
for
detection
miR-16
associated
Alzheimer's
disease,
demonstrates
ability
limit
1.7
fM.
This
work
provides
new
approach
improve
enriches
fundamental
understanding
structure–property
relationship.
The Analyst,
Journal Year:
2022,
Volume and Issue:
147(12), P. 2834 - 2842
Published: Jan. 1, 2022
Both
apurinic/apyrimidinic
endonuclease
1
(APE1)
and
microRNA-21
(miRNA-21)
have
been
reported
to
be
related
tumors,
enabling
them
the
biomarkers
of
several
cancers.
This
has
led
development
various
biosensors
detect
APE1
or
miRNA-21.
However,
that
focus
on
single
target
detection
are
subject
low
accuracy.
In
this
work,
a
fluorescent
biosensor
based
enzyme-involved
catalytic
hairpin
assembly
(CHA)
for
miRNA-21
was
developed,
aimed
at
improving
accuracy
early-phase
diagnosis
Two
structured
DNA
probes
(H1
H2)
were
utilized
concatenate
enzyme-assisted
circuit
CHA
in
system.
The
stem
H1
with
blunt
end
modified
an
AP
site,
while
H2
6-FAM
5'
terminal
Dabcyl
3'
terminal.
presence
APE1,
cleaved
from
site
expose
toehold
sequence.
Then,
bound
sequence
initiate
reaction
between
H2.
assembled
product
triggered
distance
Dabcyl,
which
recovered
fluorescence
signal.
It
is
worth
noting
only
under
co-stimulation
can
signal
detected,
indicating
could
work
as
AND
logic
gate.
proposed
dual-functional
achieved
limit
(LOD)
0.016
U
mL-1
0.25
nM
respectively,
also
exhibits
good
selectivity
stability
two
biomarkers.
Thus,
great
potential
applied
new
platform
cancer
diagnosis.
Advanced Materials,
Journal Year:
2023,
Volume and Issue:
36(22)
Published: Nov. 28, 2023
Abstract
Extracellular
vesicles
(EVs)
are
cell‐derived
nanovesicles
comprising
a
myriad
of
molecular
cargo
such
as
proteins
and
nucleic
acids,
playing
essential
roles
in
intercellular
communication
physiological
pathological
processes.
EVs
have
received
substantial
attention
noninvasive
biomarkers
for
disease
diagnosis
prognosis.
Owing
to
their
ability
recognize
protein
acid
targets,
DNA‐based
nanomaterials
with
excellent
programmability
modifiability
provide
promising
tool
the
sensitive
accurate
detection
carried
by
EVs.
In
this
perspective,
recent
advancements
EV
analysis
using
variety
summarized,
which
can
be
broadly
classified
into
three
categories:
linear
DNA
probes,
nanostructures,
hybrid
nanomaterials.
The
design,
construction,
advantages,
disadvantages
different
types
nanomaterials,
well
performance
detecting
reviewed.
challenges
opportunities
field
also
discussed.
