Angewandte Chemie,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 8, 2024
Abstract
Enzymes
encapsulated
in
extracellular
vesicles
(EVs)
hold
promise
as
biomarkers
for
early
cancer
diagnosis.
However,
precise
measurement
of
their
catalytic
activities
within
EVs
remains
a
notable
challenge.
Here,
we
report
an
enzymatically
triggered
spherical
DNA
nanomotor
(EDM)
that
enables
one‐pot,
cascaded,
and
highly
sensitive
analysis
the
activity
EV‐associated
or
free
apurinic/apyrimidinic
endonuclease
1
(APE1,
key
enzyme
base
excision
repair)
across
various
biological
samples.
The
EDM
capitalizes
on
APE1‐triggered
activation
DNAzyme
(Dz)
its
autonomous
cleavage
substrates
to
achieve
nonlinear
signal
amplification.
Using
EDM,
demonstrate
strong
correlation
between
APE1
parental
cells.
Additionally,
EV
mirrors
fluctuation
cellular
response
chemotherapy‐induced
damage.
In
pilot
clinical
study
(
n
=63),
EDM‐based
assay
reveals
more
than
80
%
active
serum
samples
is
EV‐encapsulated.
Notably,
can
differentiate
prostate
(PCa)
patients
from
healthy
donors
(HDs)
with
overall
accuracy
92
%,
outperforming
sera.
We
anticipate
will
become
versatile
tool
quantifying
enzymes.
Advanced Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 21, 2025
Diabetic
foot
ulcer
(DFU)
is
a
common
and
severe
complication
of
diabetes
mellitus,
the
etiology
which
remains
insufficiently
understood,
particularly
regarding
involvement
extracellular
vesicles
(EVs).
In
this
study,
nanoflow
cytometry
to
detect
EVs
in
DFU
skin
tissues
used
found
significant
increase
Translocase
Outer
Mitochondrial
Membrane
20
(TOM20)+
mitochondrial-derived
(MDVs).
The
role
MDVs
yet
be
reported.
Using
single-cell
datasets,
it
discovered
that
may
regulated
by
Sorting
Nexin
9
(SNX9).
vitro
experiments
revealed
secreted
fibroblasts
cultured
high
glucose
medium
exhibited
similar
composition
protein
enrichment
results
those
tissues,
suggesting
their
potential
as
an
ideal
surrogate.
These
promoted
apoptosis
intracellular
oxidative
stress,
disrupted
mitochondrial
structure,
reduced
aerobic
metabolism
target
cells.
vivo
also
showed
MDV
drops
hindered
wound
healing
diabetic
mice;
however,
effect
rescued
SNX9
inhibitors,
restoring
dynamics
balance.
Under
conditions,
significantly
upregulated
stress
levels
induced
dysfunction.
This
study
proposes
targeting
therapeutic
strategy
for
DFU.
ACS Nano,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 7, 2025
The
analysis
of
small
extracellular
vesicles
(sEVs)
has
shown
clinical
significance
in
early
cancer
diagnostics
and
considerable
potential
prognostic
assessment
therapeutic
monitoring,
offering
possibilities
for
precise
intervention.
Despite
recent
diagnostic
progress
based
on
blood-derived
sEVs,
the
inability
to
specifically
profile
multiple
parameters
sEVs
proteins
hampered
advancement
applications.
Herein,
we
report
an
approach
colorectal
(CRC)-derived
by
using
multiaptamer-triggered
rolling
circle
amplification
(RCA)
cascades.
In
practice,
presence
target
complementary
strands
are
released
from
duplexes
structure-switching
aptamer.
Then,
RCA
cascade
occurs
but
only
when
specific
DNA
strand
pair
is
presented.
As
a
result,
noncanonical
assemblies
generated
whose
size
reaches
micrometers
that
can
be
directly
analyzed
conventional
flow
cytometry,
thereby
facilitating
facile
diagnostics.
this
study,
developed
method
verified
cell-derived
followed
achieving
modeling
samples.
final
results
cohort
indicate
promising
efficacy
CRC-derived
with
92%
sensitivity,
86.7%
specificity,
90%
overall
accuracy,
highlighting
substantial
as
biomarkers
CRC
diagnosis
significantly
advancing
development
tools
disease
diagnosis.
Advanced Materials,
Journal Year:
2024,
Volume and Issue:
36(26)
Published: March 16, 2024
Abstract
Existing
tear
sensors
are
difficult
to
perform
multiplexed
assays
due
the
minute
amounts
of
biomolecules
in
tears
and
tiny
volume
tears.
Herein,
authors
leverage
DNA
tetrahedral
frameworks
(DTFs)
modified
on
wireless
portable
electrodes
effectively
capture
3D
hybridization
chain
reaction
(HCR)
amplifiers
for
automatic
sensitive
monitoring
multiple
cytokines
human
The
developed
allow
determination
various
dry
eye
syndrome
(DES)‐associated
with
limit
detection
down
0.1
pg
mL
−1
,
consuming
as
little
3
fluid.
Double‐blind
testing
clinical
DES
samples
using
sensor
commercial
ELISA
shows
no
significant
difference
between
them.
Compared
single‐biomarker
diagnosis,
diagnostic
accuracy
this
based
biomarkers
has
improved
by
≈16%.
system
offers
potential
enable
personalized
accurate
diagnosis
ocular
diseases.
Small,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 16, 2024
Abstract
Artificial
DNA
circuits
represent
a
versatile
yet
promising
toolbox
for
in
situ
monitoring
and
concomitant
regulation
of
diverse
biological
events
within
live
cells.
Nonetheless,
their
performance
is
significantly
impeded
by
the
diffusion‐dominated
slow
reaction
kinetics
uncontrollable
off‐target
activation.
Herein,
self‐localized
cascade
(SLC)
circuit
reported
robust
efficient
microRNA
(miRNA)
analysis
living
The
SLC
consists
cell‐specific
localization
module
analyte‐specific
signal
amplification
module.
By
integrating
probes
these
two
modules,
complexity
system
reduced
to
realize
responsive
co‐localization
circuitry
simultaneous
amplification.
Taking
advantage
specifically
activated,
self‐localized,
design,
successfully
achieves
miRNA‐21
(miR‐21)
imaging
accurate
cells
differentiation.
Moreover,
reverse
mechanism
explored
between
messenger
RNA
(mRNA)
miRNA
through
engineered
further
elucidates
underlying
signaling
pathways
them.
Therefore,
provides
powerful
tool
sensitive
detection
intracellular
biomolecules
study
corresponding
cell
regulatory
mechanisms.
Chemical Science,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Dec. 30, 2024
The
development
of
universal
electrochemical
sensing
platforms
with
high
sensitivity
and
specificity
is
great
significance
for
advancing
practical
disease
diagnostic
methods
devices.
Exploring
the
structural
properties
electrode
materials
their
interaction
biomolecules
essential
to
developing
novel
distinctive
analytical
approaches.
Here,
we
innovatively
investigated
effect
DNA
length
configuration
on
molecule
transfer
into
nanostructure
a
nanoporous
gold
(NPG)
electrode.
NPG
can
not
only
distinguish
quantify
short
strands
but
also
prevent
diffusion
long
DNA,
thereby
minimizing
or
eliminating
background
interference.
Leveraging
these
findings,
developed
DNA-based
biosensing
platform
detection
different
types
biomolecules.
As
proof-of-concept,
this
was
integrated
nuclease-assisted
target-recycling
recognition
amplification
reactions
achieve
sensitive
specific
single-stranded
microRNA-21,
carcino-embryonic
antigen,
limits
4.09,
27.4,
0.28
fM,
respectively.
demonstrated
universality,
sensitivity,
specificity,
capability
analyzing
complex
samples
ensure
comprehensive
robust
approach
nucleic
acid-based
molecular
diagnosis.
Angewandte Chemie International Edition,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 8, 2024
Abstract
Enzymes
encapsulated
in
extracellular
vesicles
(EVs)
hold
promise
as
biomarkers
for
early
cancer
diagnosis.
However,
precise
measurement
of
their
catalytic
activities
within
EVs
remains
a
notable
challenge.
Here,
we
report
an
enzymatically
triggered
spherical
DNA
nanomotor
(EDM)
that
enables
one‐pot,
cascaded,
and
highly
sensitive
analysis
the
activity
EV‐associated
or
free
apurinic/apyrimidinic
endonuclease
1
(APE1,
key
enzyme
base
excision
repair)
across
various
biological
samples.
The
EDM
capitalizes
on
APE1‐triggered
activation
DNAzyme
(Dz)
its
autonomous
cleavage
substrates
to
achieve
nonlinear
signal
amplification.
Using
EDM,
demonstrate
strong
correlation
between
APE1
parental
cells.
Additionally,
EV
mirrors
fluctuation
cellular
response
chemotherapy‐induced
damage.
In
pilot
clinical
study
(
n
=63),
EDM‐based
assay
reveals
more
than
80
%
active
serum
samples
is
EV‐encapsulated.
Notably,
can
differentiate
prostate
(PCa)
patients
from
healthy
donors
(HDs)
with
overall
accuracy
92
%,
outperforming
sera.
We
anticipate
will
become
versatile
tool
quantifying
enzymes.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 27, 2025
Fibroblast
phenotypic
transformation
plays
a
crucial
role
in
tumorigenesis.
Tumor
cell-derived
exosome-mediated
fibroblast
has
been
commonly
studied.
However,
there
is
still
no
direct
evidence
that
the
process
of
tumor
exosomes
induces
fibroblasts
into
cancer-associated
(CAFs).
Herein,
exosomal
miRNAs
were
used
as
target
to
track
invasion
cancer
normal
fibroblasts,
and
FAP
mRNA,
CAF
biomarker,
further
trace
effect
on
recipient
cells.
Two
DNA
tetrahedral
sensors
developed
based
hybrid
chain
reaction
signal
amplification
strategy
fluorescence
resonance
energy
transfer
mechanism.
Both
showed
high
sensitivity
good
selectivity,
with
detection
limits
4.94
6.61
pM
for
miR-1247-3p
respectively.
Moreover,
two
allowed
us
image
living
cell
postinvasion
tumor-derived
exosomes,
facilitating
visual
monitoring
phenotype
uncovering
new
perspectives
metastasis
development.
