Here,
a
novel
electrochemical
biosensor
is
proposed
based
on
triple
amplification
strategy
for
detection
of
Pb2+
and
Staphylococcus
aureus
(S.
aureus).
Dual
working
systems,
also
known
as
“turning
on”
or
off”
state,
are
ingeniously
developed
by
designing
walking
strand
DNAzyme
system.
The
presence
S.
can
trigger
the
binding/cleaving/moving
process
DNA
walker.
resulting
single-chain
regarded
toeholds,
triggering
catalytic
hairpin
assembly
reaction
to
form
dendrimers
with
plentiful
grafting
sites.
grafted
Fe-MOFs
beacons
leads
in
situ
growth
high-density
electroactive
Prussian
blue
through
conversion,
significantly
increasing
sensitivity.
Under
optimal
conditions,
ultrasensitive
achieved,
limits
down
8.5
fM
1
CFU/mL,
respectively.
Moreover,
holds
high
selectivity
reproducibility,
shows
satisfactory
recovery
rates
when
validated
using
actual
water
milk
samples.
Small,
Journal Year:
2023,
Volume and Issue:
19(23)
Published: March 14, 2023
DNA
self-assembly
has
been
developed
as
a
kind
of
robust
signal
amplification
strategy,
but
most
reported
assembly
pathways
are
programmed
to
amplify
in
one
direction.
Herein,
based
on
mutual-activated
cascade
cycle
hybridization
chain
reaction
(HCR)
and
catalytic
hairpin
(CHA),
closed
circuit
(CCC)
machine
is
for
sensitive
logic
operation
molecular
recognition.
Benefiting
from
the
synergistically
accelerated
amplification,
cyclic
enabled
computing
with
strong
significant
output
signals
even
at
weak
input
signals.
The
typical
operations
such
OR,
YES,
AND,
INHIBIT,
NOR,
NAND
gate,
conveniently
clearly
executed
this
through
rational
design
elements.
Moreover,
by
integrating
target
recognition
module
CCC
module,
proposed
further
employed
homogeneous
detection
apurinic/apyrimidinic
endonuclease
1
(APE1).
precise
exponential
facilitated
highly
selective
APE1
limit
(LOD)
7.8
×
10-5
U
mL-1
.
Besides,
normal
cells
tumor
distinguished
unambiguously
method
according
detected
concentration
difference
cellular
APE1,
which
indicates
robustness
practicability
method.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(23), P. 9570 - 9575
Published: June 1, 2024
MicroRNA
(miRNA)
and
apurinic/apyrimidinic
endonuclease
1
(APE1)
have
been
reported
to
be
closely
associated
with
cancers,
making
them
potential
crucial
biomarkers
therapeutic
targets.
However,
focusing
on
the
detection
of
a
single
target
is
not
conducive
diagnosis
prognosis
assessment
diseases.
In
this
study,
an
AND
logic-gate-based
dual-locking
hairpin-mediated
catalytic
hairpin
assembly
(DL-CHA)
was
developed
for
sensitive
specific
microRNA
APE1.
By
addition
lock
each
hairpins,
APE1
serving
as
keys,
fluorescence
signals
could
only
detected
in
presence
simultaneous
stimulation
by
miRNA-224.
This
indicated
that
biosensor
operate
logic
gate.
DL-CHA
exhibited
advantages
such
low
background,
rapid
response,
high
capability.
Therefore,
serves
novel
approach
cancer
significant
applications.
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(49), P. 18199 - 18206
Published: Nov. 30, 2023
The
entropy-driven
strategy
has
been
proposed
as
a
milestone
work
in
the
development
of
nucleic
acid
amplification
technology.
With
characteristics
an
enzyme-free,
isothermal,
and
relatively
simple
design,
it
widely
used
field
biological
analysis.
However,
is
still
challenge
to
apply
for
intracellular
target
In
this
study,
dual-entropy-driven
system
constructed
on
surface
gold
nanoparticles
(AuNPs)
developed
achieve
fluorescence
determination
imaging
microRNA-21
(miRNA-21).
internalizes
fuel
chain
avoid
complexity
extra
addition
traditional
strategy.
unique
self-locked
established
by
attaching
three-stranded
structure
two
groups
AuNPs,
where
Cy5
fluorescent
label
was
first
quenched
AuNPs.
After
miRNA-21
identified,
will
be
automatically
unlocked,
cycle
reaction
driven,
leading
recovery.
self-powered
waste-recycled
greatly
improves
automation
intelligence
process.
Under
optimal
conditions,
linear
response
range
nanosensor
ranges
from
5
pM
25
nM.
This
nanoreaction
can
realize
miRNA-21,
its
good
specificity
enables
distinguish
tumor
cells
healthy
cells.
provides
integrated
powerful
way
miRNA
analysis
shows
great
potential
biomedical
field.
Biomedical Optics Express,
Journal Year:
2024,
Volume and Issue:
15(5), P. 3366 - 3366
Published: April 18, 2024
A
surface-enhanced
Raman
scattering
(SERS)
detection
platform
was
constructed
based
on
Au
nano-dodecahedrons
(AuNDs)
functionalized
with
nucleic
acid
aptamer-specific
binding
and
self-assembly
techniques.
SERS
labels
were
prepared
by
modifying
signaling
molecules
complementary
aptamer
chains
bound
the
aptamer-functionalized
AuNDs
array.
Using
this
protocol,
limits
of
(LODs)
miR-21
miR-18a
in
serum
6.8
pM
7.6
pM,
respectively,
time
5
min.
Additionally,
detected
a
mouse
model
colorectal
cancer.
The
results
protocol
consistent
quantitative
real-time
polymerase
chain
reaction
(qRT-PCR).
This
method
provides
an
efficient
rapid
for
simultaneous
testing
miRNAs,
which
has
great
potential
clinical
value
early
cancer
(CRC).
The Analyst,
Journal Year:
2024,
Volume and Issue:
150(2), P. 362 - 370
Published: Dec. 9, 2024
Acute
myocardial
infarction
(AMI)
accounts
for
a
significant
proportion
of
global
fatalities,
and
early
detection
is
crucial
improving
patient
outcomes.
Chemistry,
Journal Year:
2023,
Volume and Issue:
5(3), P. 1815 - 1831
Published: Aug. 17, 2023
Apurinic/apyrimidinic
endonuclease
1
(APE1),
also
known
as
redox
factor-1
(Ref-1),
is
a
multifunctional
protein
that
exists
widely
in
living
organisms.
It
can
specifically
recognize
and
cleave
the
DNA
apurinic/apyrimidinic
(AP)
sites
base
excision
repair
(BER)
pathway,
well
regulate
expression
of
genes
to
activate
some
transcription
factors.
The
abnormal
disruptions
biological
functions
APE1
are
linked
number
diseases,
including
inflammation,
immunodeficiency,
cancer.
Hence,
it
extremely
desired
monitor
activity
APE1,
acquiring
thorough
understanding
healing
process
damaged
making
clinical
diagnoses.
Thanks
advent
nanotechnology,
nanodevices
used
image
with
great
sensitivity
simplicity.
In
this
review,
we
will
summarize
developments
DNA-nanotechnology-empowered
fluorescence
imaging
recent
years
for
according
different
types
probes,
which
classified
into
linear
composite
nanomaterials,
three-dimensional
(3D)
nanostructures.
We
highlight
future
research
directions
field
imaging.
