Genosensing Applications of Glassy Carbon Electrodes Modified with Multi-Walled Carbon Nanotubes Non-Covalently Functionalized with Polyarginine DOI Creative Commons
Pablo Gallay, Michael López Mujica, Soledad Bollo

et al.

Micromachines, Journal Year: 2022, Volume and Issue: 13(11), P. 1978 - 1978

Published: Nov. 15, 2022

We report the advantages of glassy carbon electrodes (GCE) modified with multi-walled nanotubes (MWCNTs) non-covalently functionalized polyarginine (PolyArg) for adsorption and electrooxidation different DNAs analytical applications resulting platform. The presence nanostructures, mainly charge PolyArg that supports them, facilitates calf-thymus salmon sperm double-stranded produces an important decrease in overvoltages oxidation guanine adenine residues a significant enhancement associated currents. As proof-of-concept possible GCE/MWCNTs-PolyArg biosensing applications, we develop impedimetric genosensor quantification microRNA-21 at femtomolar levels, using as platform immobilizing DNA probe, detection limit 3fM, sensitivity 1.544 × 103 Ω M−1, successful application enriched biological fluids.

Language: Английский

Electrochemical DNA sensors for drug determination DOI
Gennady Evtugyn, Anna Porfireva,

Svetlana V. Belyakova

et al.

Journal of Pharmaceutical and Biomedical Analysis, Journal Year: 2022, Volume and Issue: 221, P. 115058 - 115058

Published: Sept. 16, 2022

Language: Английский

Citations

14
Plasmonic aptasensor with antifouling dual-functional surface layer for lysozyme detection in food DOI Creative Commons
Noemi Bellassai, Roberta D’Agata, Giuseppe Spoto

et al.

Analytica Chimica Acta, Journal Year: 2023, Volume and Issue: 1283, P. 341979 - 341979

Published: Oct. 30, 2023

Antifouling coatings are critically necessary for optical biosensors various analytical application sectors, from medical diagnostics to foodborne pathogen detection. They help avoid non-specific protein/cell attachment on the active biosensor surface and catch analytes directly in complex media. Advances antifouling plasmonic surfaces have been mainly focused detecting clinical biomarkers real biofluids, whereas developing direct analysis of media has scarcely investigated food quality control safety. Herein, we propose a new low-fouling poly-l-lysine (PLL)-based layer an allergen protein, lysozyme, matrix using plasmon resonance. The PLL-based polymer contains densely immobilized anionic oligopeptide side chains create electric charge-balanced able repel adsorption undesired molecules surface. It also includes sparsely attached aptamer probes capturing lysozyme sources with no pre-analytical sample treatment. We optimized fabrication condition tested dual-functional evaluate its ability detect target protein selectively. developed approach allowed achieving limit detection 0.04 μg mL-1 (2.95 nM) quantification 0.13 (8.95 nM). Lysozyme was successfully quantified milk samples aptasensor without pre-treatment or isolation, illustrating device's utility.

Language: Английский

Citations

6
Programmable Molecular Signal Transmission Architecture and Reactant Regeneration Strategy Driven by EXO λ for DNA Circuits DOI
Xun Zhang, Xin Liu, Yao Yao

et al.

ACS Synthetic Biology, Journal Year: 2023, Volume and Issue: 12(7), P. 2107 - 2117

Published: July 5, 2023

The characteristics of DNA hybridization enable molecular computing through strand displacement reactions, facilitating the construction complex circuits, which is an important way to realize information interaction and processing at a level. However, signal attenuation in cascade shunt process hinders reliability calculation results further expansion circuit scale. Here, we demonstrate novel programmable exonuclease-assisted transmission architecture, where with toehold employed inhibit hydrolysis EXO λ applied circuits. We construct series variable resistance parallel constant current source, ensuring excellent orthogonal properties between input output sequences while maintaining low leakage (<5%) during reaction. Additionally, simple flexible exonuclease-driven reactant regeneration (EDRR) strategy proposed circuits voltage sources that could amplify without extra fuel strands or energy. Furthermore, effectiveness EDRR reducing processes by constructing four-node circuit. These findings offer new approach enhance systems expand scale future.

Language: Английский

Citations

5
Construction of a 3D rigidified DNA nanodevice for anti-interference and reinforced biosensing by turning nuclease into a catalyst DOI

Yinghao Cheng,

Guohui Xue,

Linwen Lan

et al.

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 237, P. 115501 - 115501

Published: June 26, 2023

Language: Английский

Citations

4
Recent Progress of Electrochemical Aptasensors toward AFB1 Detection (2018–2023) DOI Creative Commons

Despina Ciobanu,

Oana Hosu, Gheorghe Melinte

et al.

Biosensors, Journal Year: 2023, Volume and Issue: 14(1), P. 7 - 7

Published: Dec. 22, 2023

Food contaminants represent possible threats to humans and animals as severe food safety hazards. Prolonged exposure contaminated often leads chronic diseases such cancer, kidney or liver failure, immunosuppression, genotoxicity. Aflatoxins are naturally produced by strains of the fungi species

Language: Английский

Citations

4
Biogenic synthesis of nanoparticles mediated by microorganisms is a novel approach for creating antimicrobial agents DOI

Murad Muhammad,

Junaid Ahmad, Abdul Basıt

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 23 - 50

Published: Jan. 1, 2024

Language: Английский

Citations

1
Sesame Detection in Food Using DNA-Functionalized Gold Nanoparticles: A Sensitive, Rapid, and Cost-Effective Colorimetric Approach DOI Creative Commons
Pablo Llano‐Suárez, Adrián Sánchez-Visedo, Inmaculada Ortiz‐Gómez

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(8), P. 377 - 377

Published: Aug. 3, 2024

Food safety control is a key issue in the food and agriculture industries. For such purposes, developing miniaturized analytical methods critical for enabling rapid sensitive detection of supplements, allergens, pollutants. Here, novel bioanalytical methodology based on DNA-functionalized gold nanoparticles (AuNPs) colorimetric was developed to detect presence sesame (a major allergen) through seed DNA as target, samples. The induces controlled nanoparticle aggregation/desegregation, resulting color change (from blue red) proportional concentration. incorporation multicomponent nucleic acid enzymes (MNAzymes) this strategy has been carried out perform an isothermal signal amplification improve sensitivity detection. Also, open-source software analysis used ensure unbiased visual color-change detection, enhancing accuracy opening possibility performing simple decentralized analyte method successfully detected seed, oil, olive sunflower oil. In brief, approach constitutes affordable alternative highly without complex methodologies or requirement expensive instrumentation.

Language: Английский

Citations

1
Development of a TaqMan real-time PCR assay with an internal amplification control for detecting trace amounts of allergenic mango (Mangifera indica) in commercial food products DOI
Hsin‐Yi Yin, Yu‐Fan Liu, Yuyi Lin

et al.

European Food Research and Technology, Journal Year: 2023, Volume and Issue: 249(10), P. 2647 - 2660

Published: July 6, 2023

Language: Английский

Citations

3
A homogeneous hybridization magnetic biosensor based on electric field assistance for ultrafast nucleic acid detection DOI

Jinling Cui,

Ritong Sun,

Xiaoli Zhao

et al.

Analytical Methods, Journal Year: 2023, Volume and Issue: 15(27), P. 3325 - 3332

Published: Jan. 1, 2023

A homogeneous-hybridization-based DNA biosensor was constructed with electric field assistance, which quickly detects the target within 400 s superior sensitivity and specificity.

Language: Английский

Citations

2
Highly Sensitive Fluorescent Aptasensor for Detecting Α-Amatoxin Based on Rolling Circle Amplification Triggered by Aptamer-Tetrahedral DOI

Run Tian,

Yongli Ye,

Xin Lü

et al.

Published: Jan. 1, 2023

α-amatoxin is the most toxic of amatoxins and responsible for deaths from accidental ingestion poisonous mushrooms. Therefore, it particularly important to provide an efficient, simple sensitive method α-amatoxin. Here, aptamer tetrahedral functionalized magnetic bead was prepared, specifically bound on released complementary DNA(C-DNA) strands it. This strand used as a primer trigger rolling circle amplification (RCA) detection The fluorescent dye embedded between double formed by hybridization probe RCA product generate strong signal. Under optimal conditions, fluorescence signal increased with increasing concentration over dynamic range 0.01 μg/mL 5 μg/mL. limit (LOD) calculated be 7 ng/mL highly selective compared liquid chromatography tandem mass spectrometry (LC-MS/MS) detecting in spiked mushrooms urine, results were consistent. Overall, this combination DNA structure nanofabrication provides exciting solution

Language: Английский

Citations

1