Sample preparation using microfluidic technologies for non-invasive tests DOI
Cemre Oksuz, E. Alperay Tarim,

H. Ahsen Ozcan

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Evaluation of a cIEF Fractionation Workflow for Offline MS Analysis of Charge Variants of the Monoclonal Antibody Matuzumab DOI Creative Commons

Antonia Wittmann,

Yannick Wilke,

Nicolas Grammel

et al.

Electrophoresis, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 18, 2025

ABSTRACT Biological drugs like monoclonal antibodies require careful analysis and characterization to ensure product quality, safety, efficacy. Charge variants of the molecule are key interest analyzed using imaged capillary isoelectric focusing (icIEF). However, deeper these poses challenges. Two workflows for their exist: an ion‐exchange chromatography method variant collection before mass spectrometry (MS) analysis, which is labor‐intensive, direct coupling CE MS, allows detailed structural but has limitations, example, due incompatibilities with ES ionization high BGE concentrations. This study evaluates a platform that fractionates charge offline MS analysis. The suitability procedure in analytical icIEF methods converted into preparative cIEF fractionation by increasing sample concentration adding 20 mM arginine as cathodic spacer was tested. After chemical mobilization fraction collection, identity fractions determined fluorescence measurement reinjection protein‐containing fractions, previously developed method. subsequently performed. general workflow demonstrated Matuzumab. Transferring from 0.2 1.2 mg/mL yielded identical number peaks visually comparable peak profiles. separation took 50 min, additional 25 min 45 s per totaling approximately 2.5 h. Verification isolation straightforward via icIEF. Following fractionation, allowed identification main peaks. Preliminary results other indicated range experiments needs further investigation. Future work will aim optimize sensitivity, selectivity, time, reproducibility.

Language: Английский

Citations

0
Closed-Loop Peristaltic Micro-Pump for Flow-Through Sample Injection in Capillary Electrophoresis DOI
Jinli Zhang, Hui Zeng,

Wenshan Liang

et al.

Talanta, Journal Year: 2025, Volume and Issue: unknown, P. 128240 - 128240

Published: May 1, 2025

Language: Английский

Citations

0
Recent developments of imaged capillary isoelectric focusing technology for in-depth biopharmaceutical characterization DOI Creative Commons
Bo Tao, Feng Yang, Bo Yan

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118212 - 118212

Published: Feb. 1, 2025

Language: Английский

Citations

0
Advancing protein heterogeneity analysis: nano-flow pressure mobilization for precise icIEF fractionation and online MS detection DOI

Teresa Kwok,

She Lin Chan,

Niusheng Xu

et al.

Analytical Biochemistry, Journal Year: 2025, Volume and Issue: 701, P. 115825 - 115825

Published: March 2, 2025

Language: Английский

Citations

0
Microfluidics-interfaced capillary electrophoresis coupled with modular LED-based fluorescent detection: A new tool for continuous monitoring of the interaction between nanoparticles and bio-entities DOI Creative Commons

Javeria Zafar,

Claire Smadja,

Jacques Fabre

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: 439, P. 137841 - 137841

Published: April 25, 2025

Language: Английский

Citations

0
High-Efficiency Enzyme Assay and Screening of Enzyme-Inhibiting Nanomaterials Using Capillary Electrophoresis with Hierarchically Porous Metal–Organic Framework-Based Immobilized Enzyme Microreactor DOI

Meiting Yan,

Dan Wang,

Hongyan Liao

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(43), P. 17300 - 17309

Published: Oct. 16, 2024

Enzyme-inhibiting nanomaterials have significant potential for regulating enzyme activity. However, a universal and efficient method systematically screening evaluating the inhibitory effects of various on drug target enzymes has not been established. While integrated technique immobilized microreactor (IMER) with capillary electrophoresis (CE) serves as an effective tool analysis, it still faces challenges such low loadability, unsatisfactory stability, limited applicability. Herein, hierarchical porous metal–organic frameworks (HP-MOFs) were explored high-performance immobilization carriers stationary phases to develop novel HP-MOFs-based IMER-CE microanalysis system online assay systematic enzyme-inhibiting nanomaterials. As proof-of-concept demonstration, model xanthine oxidase (XOD) was HP-UiO-66-NH2 coated capillary, serving durable IMER XOD-inhibiting The hierarchically micro- mesoporous structure superior loadability as-prepared HP-UiO-66-NH2–IMER intensively characterized, followed by evaluation separation performance column kinetics XOD. Compared microporous UiO-66-NH2–IMER, HP-UiO-66-NH2–IMER-CE showed improvements in loading, maximum reaction rate, repeatability, long-term stability. Furthermore, established effectively employed screen XOD activity nanomaterials, revealing that graphene oxide, single wall carbon nanotube three other exhibited potentials. can be easily expanded modifying types holds accelerate identification rational design

Language: Английский

Citations

3
Fluorescence detection setups in capillary electrophoresis and microscale liquid chromatography: Developments over the past decade DOI
Jozef Šesták,

András Guttman,

Jana Lavická

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 181, P. 118001 - 118001

Published: Oct. 8, 2024

Language: Английский

Citations

2
Capillary electromigration methods for food analysis and Foodomics: Advances and applications in the period March 2021 to March 2023 DOI Creative Commons
Gloria Domínguez‐Rodríguez, Lídia Montero, Miguel Herrero

et al.

Electrophoresis, Journal Year: 2023, Volume and Issue: 45(1-2), P. 8 - 34

Published: Aug. 21, 2023

This work presents a revision of the main applications capillary electromigration (CE) methods in food analysis and Foodomics. Papers that were published during period March 2021 to 2023 are included. The shows multiple CE have been developed applied analyze different types molecules foods beverages. Namely, amino acids, biogenic amines, heterocyclic peptides, proteins, phenols, polyphenols, pigments, lipids, carbohydrates, vitamins, DNAs, contaminants, toxins, pesticides, additives, residues, small organic inorganic compounds, other minor compounds. In addition, new procedures perform chiral separation for evaluating effects processing as well last developments microchip Foodomics will be also discussed. investigate quality safety, nutritional value, storage, bioactivity included present review work.

Language: Английский

Citations

6
Compact contactless conductometric, ultraviolet photometric and dual-detection cells for capillary electrophoresis via additive manufacturing DOI
Bangjie Yin, Zheng Zhang, Yingchun Wang

et al.

Journal of Chromatography A, Journal Year: 2023, Volume and Issue: 1712, P. 464469 - 464469

Published: Oct. 24, 2023

Language: Английский

Citations

6
Imaged Capillary Isoelectric Focusing Method Development for Charge Variants of High DAR ADCs DOI
Chuan Leng, Shu-Wen Sun,

Wei Lin

et al.

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1328, P. 343176 - 343176

Published: Aug. 28, 2024

Language: Английский

Citations

1