bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2022,
Volume and Issue:
unknown
Published: Aug. 16, 2022
ABSTRACT
Control
of
cellular
identity
requires
coordination
developmental
programs
with
environmental
factors
such
as
nutrient
availability,
suggesting
that
modulating
aspects
metabolism
could
alter
cell
state
along
differentiation
trajectories.
Here
we
find
nucleotide
depletion
and
DNA
replication
stress
are
common
drivers
progression
across
a
variety
normal
transformed
hematopoietic
systems.
stress-induced
transitions
begin
during
S
phase
independent
ATR/ATM
checkpoint
signaling,
double-stranded
break
formation,
changes
in
cycle
length.
In
systems
where
is
blocked
by
oncogenic
transcription
factor
expression,
leads
to
increased
activity
at
primed
regulatory
loci
expression
lineage-appropriate
maturation
genes
while
progenitor
TF
still
present.
Altering
the
baseline
manipulating
cohort
expressed
redirects
effect
towards
induction
different
set
lineage-specific
genes.
The
ability
selectively
activate
contexts
suggests
general
mechanism
which
can
promote
potentially
therapeutically
relevant
transitions.
Frontiers in Immunology,
Journal Year:
2024,
Volume and Issue:
15
Published: Jan. 29, 2024
Cancer,
a
disease
that
modern
medicine
has
not
fully
understood
and
conquered,
with
its
high
incidence
mortality,
deprives
countless
patients
of
health
even
life.
According
to
global
cancer
statistics,
there
were
an
estimated
19.3
million
new
cases
nearly
10
deaths
in
2020,
the
age-standardized
mortality
rates
201.0
100.7
per
100,000,
respectively.
Although
remarkable
advancements
have
been
made
therapeutic
strategies
recently,
overall
prognosis
remains
optimistic.
Consequently,
are
still
many
severe
challenges
be
faced
difficult
problems
solved
therapy
today.
Epigallocatechin
gallate
(EGCG),
natural
polyphenol
extracted
from
tea
leaves,
received
much
attention
for
antitumor
effects.
Accumulating
investigations
confirmed
EGCG
can
inhibit
tumorigenesis
progression
by
triggering
apoptosis,
suppressing
proliferation,
invasion,
migration,
altering
tumor
epigenetic
modification,
overcoming
chemotherapy
resistance.
Nevertheless,
regulatory
roles
biomolecular
mechanisms
immune
microenvironment,
metabolic
immunotherapy
remain
obscure.
In
this
article,
we
summarized
most
recent
updates
about
effects
on
microenvironment
(TME),
reprogramming,
anti-cancer
immunotherapy.
The
results
demonstrated
promote
response
cytotoxic
lymphocytes
dendritic
cells
(DCs),
attenuate
immunosuppression
myeloid-derived
suppressor
(MDSCs)
T
(Tregs),
tumor-promoting
functions
tumor-associated
macrophages
(TAMs),
neutrophils
(TANs),
various
stromal
including
cancer-associated
fibroblasts
(CAFs),
endothelial
(ECs),
stellate
cells,
mesenchymal
stem/stromal
(MSCs).
Additionally,
suppress
multiple
reprogramming
pathways,
glucose
uptake,
aerobic
glycolysis,
glutamine
metabolism,
fatty
acid
anabolism,
nucleotide
synthesis.
Finally,
EGCG,
as
immunomodulator
checkpoint
blockade,
enhance
immunotherapeutic
efficacy
may
promising
candidate
conclusion,
plays
versatile
TME
which
provides
novel
insights
combined
MedComm – Oncology,
Journal Year:
2025,
Volume and Issue:
4(1)
Published: Feb. 17, 2025
ABSTRACT
Metastasis
remains
a
leading
cause
of
cancer‐related
deaths,
defined
by
complex,
multi‐step
process
in
which
tumor
cells
spread
and
form
secondary
growths
distant
tissues.
Despite
substantial
progress
understanding
metastasis,
the
molecular
mechanisms
driving
this
development
effective
therapies
remain
incompletely
understood.
Elucidating
pathways
governing
metastasis
is
essential
for
discovery
innovative
therapeutic
targets.
The
rapid
advancements
sequencing
technologies
expansion
biological
databases
have
significantly
deepened
our
drivers
associated
drug
resistance.
This
review
focuses
on
particularly
roles
genetic
mutations,
epigenetic
changes,
post‐translational
modifications
progression.
We
also
examine
how
microenvironment
influences
metastatic
behavior
explore
emerging
strategies,
including
targeted
immunotherapies.
Finally,
we
discuss
future
research
directions,
stressing
importance
novel
treatment
approaches
personalized
strategies
to
overcome
improve
patient
outcomes.
By
integrating
contemporary
insights
into
basis
innovation,
provides
comprehensive
framework
guide
clinical
cancer.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(12), P. 6735 - 6735
Published: June 19, 2024
Tumor
cells
reprogram
their
metabolism
to
meet
the
increased
demand
for
nucleotides
and
other
molecules
necessary
growth
proliferation.
In
fact,
cancer
are
characterized
by
an
"de
novo"
synthesis
of
purine
nucleotides.
Therefore,
it
is
not
surprising
that
specific
enzymes
targets
drugs
as
antineoplastic
agents,
a
better
knowledge
mechanisms
underlying
regulation
would
be
great
help
in
finding
new
therapeutic
approaches.
The
mammalian
target
rapamycin
(mTOR)
signaling
pathway,
which
often
activated
cells,
promotes
anabolic
processes
major
regulator
cell
division.
Among
numerous
effects
exerted
mTOR,
noteworthy
its
empowerment
nucleotides,
accomplished
supporting
formation
purinosomes,
increasing
availability
precursors,
such
one-carbon
formyl
group,
bicarbonate
5-phosphoribosyl-1-pyrophosphate.
this
review,
we
highlight
connection
between
mitochondrial
metabolism,
bidirectional
relation
mTOR
pathways.
Colorectal
cancer
is
a
malignant
tumor
of
the
digestive
system
originating
from
abnormal
cell
proliferation
in
colon
or
rectum,
often
leading
to
gastrointestinal
symptoms
and
severe
health
issues.
Nucleotide
metabolism,
which
encompasses
synthesis
DNA
RNA,
pivotal
cellular
biochemical
process
that
significantly
impacts
both
progression
therapeutic
strategies
colorectal
METHODS:
For
single-cell
RNA
sequencing
(scRNA-seq),
five
functions
were
employed
calculate
scores
related
nucleotide
metabolism.
Cell
developmental
trajectory
analysis
intercellular
interaction
utilized
explore
metabolic
characteristics
communication
patterns
different
epithelial
cells.
These
findings
further
validated
using
spatial
transcriptome
(stRNA-seq).
A
risk
model
was
constructed
expression
profile
data
TCGA
GEO
cohorts
optimize
clinical
decision-making.
Key
metabolism-related
genes
(NMRGs)
functionally
by
vitro
experiments.
