Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: unknown, P. 107056 - 107056
Published: Oct. 1, 2024
Language: Английский
Microorganisms, Journal Year: 2024, Volume and Issue: 12(4), P. 772 - 772
Published: April 11, 2024
Antibiotic resistance has emerged as a significant global public health issue, driven by the rapid adaptation of microorganisms to commonly prescribed antibiotics. Colistin, previously regarded last-resort antibiotic for treating infections caused Gram-negative bacteria, is increasingly becoming resistant due chromosomal mutations and acquisition genes carried plasmids, particularly mcr genes. The mobile colistin gene (mcr-1) was first discovered in E. coli from China 2016. Since that time, studies have reported different variants ranging mcr-1 mcr-10, mainly Enterobacteriaceae various parts world, which major concern health. co-presence colistin-resistant with other determinants further complicates treatment strategies underscores urgent need enhanced surveillance antimicrobial stewardship efforts. Therefore, understanding mechanisms driving monitoring its prevalence are essential steps addressing growing threat preserving efficacy existing This review critical role last-choice antibiotic, elucidates dissemination genes, explores evaluates current detection methods bacteria. objective shed light on these key aspects combating
Language: Английский
Citations
58
Antibiotics, Journal Year: 2024, Volume and Issue: 13(2), P. 195 - 195
Published: Feb. 19, 2024
Urinary tract infections (UTIs) caused by resistant Klebsiella pneumoniae can lead to severe clinical complications and even death. An alternative treatment option for infected patients is using bacteriophages. In the present study, we isolated phage VB_KPM_KP1LMA (KP1LMA) from sewage water a K. strain as host. Whole-genome analysis indicated that genome was double-stranded linear 176,096-bp long DNA molecule with 41.8% GC content did not contain virulence or antibiotic resistance genes. The inactivation potential of KP1LMA assessed in broth at an MOI 1 10, maximum 4.9 5.4 log CFU/mL, respectively, observed after 9 h. efficacy 10 also urine evaluate phage’s performance acidic environment. A 3.8 CFU/mL results suggest could potentially control UTI this pneumoniae, indicating same procedure be used UTIs other strains if new specific phages are isolated. Although has narrow host range, future, efforts made expand its spectrum activity combine others, enabling use against involved UTIs.
Language: Английский
Citations
7
Virology Journal, Journal Year: 2024, Volume and Issue: 21(1)
Published: March 6, 2024
Abstract Background Southwest China is one of the largest karst regions in world. Karst environment relatively fragile and vulnerable to human activities. Due discharge sewage domestic garbage, system may be polluted by pathogenic bacteria. The detection bacterial distribution identification phage capable infecting them an important approach for environmental assessment resource acquisition. Methods Bacteria phages were isolated from water southwest using plate scribing double method, respectively. Isolated was defined transmission electron microscopy, one-step growth curve optimal multiplicity infection (MOI). Genomic sequencing, phylogenetic analysis, comparative genomic proteomic analysis performed. Results A Klebsiella quasipneumoniae 32 isolates named KL01. KL01 morphologically identified as Caudoviricetes with MOI 0.1, incubation period 10 min, a lysis 60 min. genome length about 45 kb, GC content 42.5%, it contains 59 open reading frames. highest average nucleotide similarity between known 6939 83.04%. Conclusions novel phage, belonging Autophagoviridae , which has strong lytic ability. This study indicates that there not only some potential potentially bacteria environment, but also resources exploration application.
Language: Английский
Citations
4
PLoS ONE, Journal Year: 2025, Volume and Issue: 20(2), P. e0315079 - e0315079
Published: Feb. 21, 2025
Background The increasing incidence of AmpC β-lactamase producing by K . pneumoniae has raised global alarm. Consequently, there is a crucial need for effective methods to inactivate pathogenic bacteria and mitigate the associated risks. Bacteriophage therapy been demonstrated be an alternative approach targeting inactivating that produces AmpC. This study aimed isolate characterize Klebsiella AmpC-specific phages from hospital wastewater. Methods wastewater samples were collected sewage water effluent tertiary at Universiti Sains Malaysia, located on east coast Malaysia. These underwent serial filtration centrifugation processes phage recovery. solutions undergoing screening test spot assay using clinical isolates strain as amplification hosts. isolated AmpC-phages further studied characterised determine phage’s host range, temperature, pH, chloroform stabilities. High-Resolution Transmission Electron Microscopy (HRTEM) was performed type. Results Thirty HWW analyzed four K. strains resulting in total 120 plates. AmpC—Klebsiella (AmpC-KP) detected 31.70% (38/120) AmpC-KP had lytic diameters ranging 1–3 mm, titer ranged from4×10 3 –3.2×10 7 PFU/ml. narrow–host range stable temperature -20–50˚C. also pH 4 9 different concentrations (5%,10%). Based HRTEM, Siphoviridea identified. Conclusions abundant wastewater, good source phages. high effectivity specificity with narrow could survive under harsh conditions such (temperature, chloroform).
Language: Английский
Citations
0
AMB Express, Journal Year: 2025, Volume and Issue: 15(1)
Published: March 5, 2025
The rise of deaths by resistant bacteria is a global threat to public health systems. Klebsiella pneumoniae virulent pathogen that causes serious nosocomial infections. major obstacle bacterial treatment antibiotic resistance, which necessitates the introducing alternative therapies. Phage therapy has been regarded as promising avenue fight multidrug-resistant (MDR) pathogens. In current study, novel phage vB_KpnP_KP17 was isolated from sewage, and its lytic potential investigated against K. pneumoniae. vB_KpnP_kP17 17.5% tested isolates. One step growth curve indicated with short latent period (20 min) large burst size (331 PFU/cell). Additionally, maintained activity planktonic cells over wide range pH, temperature UV irradiation intervals. antibiofilm agent revealed biofilm inhibition assay. at multiplicity infection (MOI) 10 inhibited more than 50% attached biofilms genome characterized found be linear dsDNA 39,936 bp in length GC content 52.85%. absence toxicity, virulence resistance genes further confirms safety for clinical applications. These characteristics make therapeutic value manage MDR formulation cocktail other phages or antibiotics could applied limit biofilm-producing
Language: Английский
Citations
0
PLoS Pathogens, Journal Year: 2025, Volume and Issue: 21(4), P. e1012971 - e1012971
Published: April 8, 2025
Klebsiella pneumoniae (KP) is a Gram-negative bacterium that commonly resides in the human gastrointestinal tract and can also act as an opportunistic pathogen cause extra-intestinal infections. KP poses global health threat because it causes both hospital- community-acquired infections immune-competent immunocompromised hosts. These be multidrug-resistant and/or hypervirulent, making difficult to treat deadly. In absence of effective treatments for recalcitrant infections, bacteriophage (phage) therapy gaining attention promising alternative. this review, we evaluate epidemiology epitope diversity, discuss interactions between KP-targeting phages their bacterial hosts from eco-evolutionary perspective, summarize recent efforts phage treating We novel approaches, including genetic engineering machine learning, initial steps toward developing precision medicine approach emerging dangerous pathogen.
Language: Английский
Citations
0
Journal of Global Antimicrobial Resistance, Journal Year: 2025, Volume and Issue: unknown
Published: April 1, 2025
The emergence of extended-spectrum β-lactamase (ESBL)-producing E. coli and carbapenem-resistant (CREC) is a significant global health challenge. This study focuses on isolating characterizing two novel phages, EC.W1-9 EC.W15-4, investigating their efficacy with antibiotics against these resistant coli. In vivo experiments were conducted using BALB/c mice, E.coli isolates collected, cultured, evaluated for antibiotic susceptibility. Phages isolated from hospital sewage prepared to infect the belonged Podoviridae Straboviridae families, lack integrase or toxin-coding genes, indicating safety therapeutic use. combination phages significently enhanced lytic ability, lysing 61.7% 60 isolates, compared 41.6%-55% lysis by individual phages. Furthermore, phage demonstrated 100% susceptibility different sequence types, including ST73, ST648, ST2311, ST405, ST7962, ST131, ST13003, ST167. Additionally, synergy between combinations improved rates 73.3% ESBL producers 54% CREC. combined treatment significantly increased survival in mice exposed STs E.coli, ST410. Survival ST131 approximately 75% 50% Combined resulted 75-100% ST410 ST648 CONCLUSIONS: highlights importance phage-antibiotic combating ESBL-producing CREC isolates.
Language: Английский
Citations
0
Future Virology, Journal Year: 2024, Volume and Issue: 19(5), P. 199 - 213
Published: March 23, 2024
Aim: Bacteriophages are potential candidates for the treatment of multidrug-resistant bacterial infections. We aimed to isolate and characterize phages effective against carbapenem-resistant Acinetobacter baumannii. Materials & methods: Water samples were taken phage isolation. One-step growth curves, morphologies, multiplicity infections, adsorption assays, thermal pH stabilities molecular characterizations evaluated. Results: Four isolated characterized, including their latent period, burst sizes, length contractile tails distinct digestion profiles. Phage genomes double-stranded varied in size from 45,076 46,549 bp. No known virulence, antibiotic resistance genes or tRNA found. Conclusion: Evaluated this study promising further vivo experiments usage as therapeutic agents.
Language: Английский
Citations
2
Antimicrobial Agents and Chemotherapy, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 10, 2024
ABSTRACT Antibiotic combination therapy is a promising approach to address the urgent need for novel treatment options infections caused by carbapenem-polymyxin-resistant Klebsiella pneumoniae (CPR-Kp). The present study aimed investigate synergistic potential of four cephalosporins in with polymyxin B (PMB). A checkerboard assay was performed evaluate effects cephalexin (CLX), cefixime, cefotaxime (CTX), and cefmenoxime (CMX) PMB. Subsequently, experiments evaluating use CTX or CMX PMB (CTX-PMB CMX-PMB, respectively), including growth curve SynergyFinder analysis, antibiofilm activity assays, cell membrane integrity scanning electron microscopy, were performed. Safety assessments also conducted, hemolysis toxicity evaluations, using Caenorhabditis elegans . Furthermore, an vivo model C. adopted assess efficacy against CPR-Kp infections. CTX-PMB CMX-PMB exhibited low fractional inhibitory concentration indexes ranging from 0.19 0.50 0.25 1.5, respectively, zero interaction potency scores 37.484 15.076, respectively. two combinations significantly reduced biofilm formation CPR-Kp. Neither nor compromised bacterial integrity. revealed percentage high survival rates evaluations. that treatments improved combinations, both vitro , indicate these antibiotic pairings could represent effective therapeutic
Language: Английский
Citations
2
Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15
Published: Sept. 17, 2024
Carbapenem-resistant (CR) Gram-negative bacteria have become a significant public health problem in the last decade. In recent years, prevalence of CR has increased. The resistance to carbapenems could result from different mechanisms such as loss porin, penicillin-binding protein alteration, carbapenemase, efflux pump, and biofilm community. Additionally, genetic variations like insertion, deletion, mutation, post-transcriptional modification corresponding coding genes decrease susceptibility carbapenems. this regard, scientists are looking for new approaches inhibit bacteria. Using bacteriophages, natural products, nanoparticles, disulfiram, N-acetylcysteine, antimicrobial peptides showed promising inhibitory effects against mentioned compounds destroy community them combination with conventional antibiotics increases efficacy antibiotics, decreases their dosage toxicity, resensitizes antibiotics. Therefore, present review article, we discussed aspects non-antibiotic managing inhibiting various methods procedures used an alternative these
Language: Английский
Citations
1