Within- and between-Day Variability of SARS-CoV-2 RNA in Municipal Wastewater during Periods of Varying COVID-19 Prevalence and Positivity DOI
Aaron Bivins, Devin North, Zhenyu Wu

et al.

ACS ES&T Water, Journal Year: 2021, Volume and Issue: 1(9), P. 2097 - 2108

Published: Aug. 13, 2021

Wastewater surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA is being used to monitor Coronavirus Disease 2019 (COVID-19) trends in communities; however, within- and between-day variation SARS-CoV-2 concentration primary influent remains largely uncharacterized. In the current study, grab sampling was performed every h over two 24-h periods at wastewater treatment plants (WWTPs) northern Indiana, USA. The recovery efficiency endogenous confirmed be similar process control, bovine syncytial virus (BRSV). Recovery-corrected concentrations indicate diurnal loading patterns confirm monitoring dependent on samples should target daytime with high fecal loading. Importantly, manual compositing WWTP resulted that were consistently lower than sample averages indicating potential bias. Uncorrected, recovery-corrected, pepper mild mottle (PMMoV)-normalized demonstrated an ordinal agreement increasing clinical COVID-19 positivity but not cases. areas where geolocated case data are available, rate could provide a useful county-level metric for comparison wastewater. Nonetheless, large both between-days may preclude robust quantitative analyses beyond correlation.

Language: Английский

Evaluation of Sampling, Analysis, and Normalization Methods for SARS-CoV-2 Concentrations in Wastewater to Assess COVID-19 Burdens in Wisconsin Communities DOI Creative Commons
Shuchen Feng, Adélaïde Roguet,

Jill S. McClary-Gutierrez

et al.

ACS ES&T Water, Journal Year: 2021, Volume and Issue: 1(8), P. 1955 - 1965

Published: July 9, 2021

Wastewater surveillance for SARS-CoV-2 provides an approach assessing the infection burden across a sewer service area. For these data to be useful public health, measurement variability and relationship case need established. We determined RNA concentrations in influent of 12 wastewater treatment plants from August 2020 January 2021. Technical replicates N1 gene showed relative standard deviation 24%, suggesting it is possible track relatively small (∼30%) changes over time. COVID-19 cases were correlated significantly (ρ ≥ 0.70) large areas, with weaker relationships 0.59) two communities. normalized per capita slightly improved correlations incidence, but normalizing spiked recovery control (BCoV) or fecal marker (PMMoV HF183) reduced number plants. Daily sampling demonstrated that minimum samples collected week needed maintain accuracy trend analysis. The differences strength incidence effect normalization on among communities demonstrate rigorous validation should performed at individual sites where programs are implemented.

Language: Английский

Citations

223
Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance DOI Creative Commons
Warish Ahmed, Stuart L. Simpson, Paul M. Bertsch

et al.

The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 805, P. 149877 - 149877

Published: Aug. 25, 2021

Wastewater surveillance for pathogens using reverse transcription-polymerase chain reaction (RT-PCR) is an effective and resource-efficient tool gathering community-level public health information, including the incidence of coronavirus disease-19 (COVID-19). Surveillance Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) in wastewater can potentially provide early warning signal COVID-19 infections a community. The capacity world's environmental microbiology virology laboratories SARS-CoV-2 RNA characterization increasing rapidly. However, there are no standardized protocols or harmonized quality assurance control (QA/QC) procedures surveillance. This paper technical review factors that cause false-positive false-negative errors wastewater, culminating recommended strategies be implemented to identify mitigate some these errors. Recommendations include stringent QA/QC measures, representative sampling approaches, virus concentration efficient extraction, PCR inhibition assessment, inclusion sample processing controls, considerations RT-PCR assay selection data interpretation. Clear interpretation guidelines (e.g., determination positive negative samples) critical, particularly when low. Corrective confirmatory actions must place inconclusive results diverging from current trends initial onset reemergence community). It also prudent perform interlaboratory comparisons ensure results' reliability interpretability prospective retrospective analyses. this aim improve detection applications. A silver lining pandemic efficacy continues demonstrated during global crisis. In future, should play important role range other communicable diseases.

Language: Английский

Citations

222
Making waves: Plausible lead time for wastewater based epidemiology as an early warning system for COVID-19 DOI Open Access
Kyle Bibby, Aaron Bivins, Zhenyu Wu

et al.

Water Research, Journal Year: 2021, Volume and Issue: 202, P. 117438 - 117438

Published: July 12, 2021

Language: Английский

Citations

129
SARS-CoV-2 Wastewater Surveillance for Public Health Action DOI Creative Commons

Jill S. McClary-Gutierrez,

Mia Mattioli,

Perrine Marcenac

et al.

Emerging infectious diseases, Journal Year: 2021, Volume and Issue: 27(9), P. 1 - 8

Published: Aug. 19, 2021

Abstract Wastewater surveillance for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has garnered extensive public attention during the disease pandemic as a proposed complement to existing systems. Over past year, methods detection and quantification of SARS-CoV-2 viral RNA in untreated sewage have advanced, concentrations wastewater been shown correlate with trends reported cases. Despite promise surveillance, these measurements translate into useful health tools, bridging communication knowledge gaps between researchers responders is needed. We describe key uses, barriers, applicability supporting decisions actions, including establishing ethics consideration monitoring. Although assess community infections not new idea, might be initiating event make this emerging tool sustainable nationwide system, provided that barriers are addressed.

Language: Английский

Citations

116
Municipal and neighbourhood level wastewater surveillance and subtyping of an influenza virus outbreak DOI Creative Commons
Élisabeth Mercier, Patrick M. D’Aoust, Ocean Thakali

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: Sept. 22, 2022

Recurrent influenza epidemics and pandemic potential are significant risks to global health. Public health authorities use clinical surveillance locate monitor influenza-like cases outbreaks mitigate hospitalizations deaths. Currently, integration of is the only reliable method for reporting types subtypes warn emergent strains. The utility wastewater (WWS) during COVID-19 as a less resource intensive replacement or complement has been predicated on analyzing viral fragments in wastewater. We show here that virus targets stable partitions favorably solids fraction. By quantifying, typing, subtyping municipal primary sludge community outbreak, we forecasted citywide flu outbreak with 17-day lead time provided population-level near real-time feasibility WWS at neighbourhood levels real using minimal resources infrastructure.

Language: Английский

Citations

110
Comparison of virus concentration methods and RNA extraction methods for SARS-CoV-2 wastewater surveillance DOI

Xiawan Zheng,

Yu Deng, Xiaoqing Xu

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 824, P. 153687 - 153687

Published: Feb. 5, 2022

Language: Английский

Citations

85
Relationships between SARS-CoV-2 in Wastewater and COVID-19 Clinical Cases and Hospitalizations, with and without Normalization against Indicators of Human Waste DOI Creative Commons

Qingyu Zhan,

Kristina M. Babler,

Mark Sharkey

et al.

ACS ES&T Water, Journal Year: 2022, Volume and Issue: 2(11), P. 1992 - 2003

Published: May 26, 2022

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in wastewater has been used to track community infections of coronavirus disease-2019 (COVID-19), providing critical information for public health interventions. Since levels are dependent upon human inputs, we hypothesize that tracking can be improved by normalizing concentrations against indicators waste [Pepper Mild Mottle Virus (PMMoV), β-2 Microglobulin (B2M), and fecal coliform]. In this study, analyzed SARS-CoV-2 from two sewersheds different scales: a University campus treatment plant. Wastewater data were combined with complementary COVID-19 case evaluate the efficiency surveillance forecasting new cases and, larger scale, hospitalizations. Results show normalization PMMoV B2M resulted correlations using volcano second generation (V2G)-qPCR chemistry (rs = 0.69 without normalization, rs 0.73 normalization). Mixed results obtained samples collected at scale. Overall benefits measures depend qPCR improves smaller sewershed We recommend further studies efficacy additional targets.

Language: Английский

Citations

81
Does normalization of SARS-CoV-2 concentrations by Pepper Mild Mottle Virus improve correlations and lead time between wastewater surveillance and clinical data in Alberta (Canada): comparing twelve SARS-CoV-2 normalization approaches DOI Open Access
Rasha Maal‐Bared, Yuanyuan Qiu, Qiaozhi Li

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 856, P. 158964 - 158964

Published: Sept. 24, 2022

Language: Английский

Citations

78
Recent progress on wastewater-based epidemiology for COVID-19 surveillance: A systematic review of analytical procedures and epidemiological modeling DOI Open Access
Stéfano Ciannella, Cristina González-Fernández, Jenifer Gómez‐Pastora

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 878, P. 162953 - 162953

Published: March 21, 2023

Language: Английский

Citations

60
Wastewater sequencing reveals community and variant dynamics of the collective human virome DOI Creative Commons
Michael J. Tisza,

Sara Javornik Cregeen,

Vasanthi Avadhanula

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Oct. 28, 2023

Wastewater is a discarded human by-product, but its analysis may help us understand the health of populations. Epidemiologists first analyzed wastewater to track outbreaks poliovirus decades ago, so-called wastewater-based epidemiology was reinvigorated monitor SARS-CoV-2 levels while bypassing difficulties and pit falls individual testing. Current approaches overlook activity most viruses preclude deeper understanding virome community dynamics. Here, we conduct comprehensive sequencing-based 363 longitudinal samples from ten distinct sites in two major cities. Critical detection use viral probe capture set targeting thousands species or variants. Over 450 pathogenic 28 families are observed, which have never been detected such samples. Sequencing reads established pathogens emerging correlate clinical data sets SARS-CoV-2, influenza virus, monkeypox viruses, outlining public utility this approach. Viral communities tightly organized by space time. Finally, abundant yield sequence variant information consistent with regional spread evolution. We reveal landscape potential improve our outbreaks, transmission, effects on overall population health.

Language: Английский

Citations

55