Characteristics of Gut Microbiota and Plasma Metabolites in Patients with Post-Stroke Depression DOI Creative Commons
Chuming Yan,

Tong Si,

Wancheng Zheng

et al.

Neuropsychiatric Disease and Treatment, Journal Year: 2025, Volume and Issue: Volume 21, P. 477 - 489

Published: March 1, 2025

The changes in gut microbiota and plasma metabolites have been proposed to play a key role post stroke depression (PSD), but clinical study based on combined omics is still lack. This aimed investigate the characteristics of patients 3 months after onset acute ischemic (AIS), compare PSD non-PSD groups, explore possible diagnostic biomarkers. Seventy with were included at AIS onset. Plasma fecal samples collected. Gut microbiome was examined using 16S rRNA sequencing, assessed via targeted liquid chromatography-mass spectrometry. Of 70 stroke, 25 (35.71%) diagnosed PSD. At genus level, had increased abundance Parabacteroides, Pyramidobacter, Anaeroglobus, Haliangium, Staphylococcus, CAG-56, Shuttleworthia, Epulopiscium, decreased levels Eubacterium eligens group Prevotella. In PSD, 12 altered, cortisol pyroglutamic acid increased, while 2-phosphoglyceric acid, 3-phosphoglycerate, phosphorylcholine, tryptophan, caffeine, N-methylalanine, ornithine, serotonin, theophylline, vanillic decreased. Enriched metabolic pathways glutathione, caffeine metabolism. Furthermore, significant correlations observed between microbial dysregulation major metabolite alterations. areas under curve values microbiota, metabolites, dataset for diagnosis 0.704, 0.875, 0.940, respectively. identified as well panel biomarkers 3-month possibly providing new theoretical framework treatment.

Language: Английский

A nanoenzyme-modified hydrogel targets macrophage reprogramming-angiogenesis crosstalk to boost diabetic wound repair DOI Creative Commons
Yang Zi, Zhenhao Li, Lu Wang

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 35, P. 17 - 30

Published: Jan. 21, 2024

Diabetic wounds has a gradually increasing incidence and morbidity. Excessive inflammation due to immune imbalance leads delayed wound healing. Here, we reveal the interconnection between activation of NLRP3 inflammatory pathway in endotheliocyte polarization macrophages via cGAS-STING oxidative microenvironment. To enhance immune-regulation based on repairing mitochondrial damage, zeolitic imidazolate framework-8 coated with cerium dioxide that carries Rhoassociated protein kinase inhibition Y-27632 (CeO2–Y@ZIF-8) is developed. It encapsulated photocross-linkable hydrogel (GelMA) cationic quaternary ammonium salt groups modified endow antibacterial properties (CeO2–Y@ZIF-8@Gel). CeO2 superoxide dismutase catalase activities can remove excess reactive oxygen species limit damage repair damaged DNA, thus improving proliferation endotheliocyte. After uptakes CeO2–Y@ZIF-8 NPs degrade peroxides into water cells mitochondria, inhibited leakage oxidatively DNA (Ox-mtDNA, damage-associated molecular pattern) through mPTP decreases. Futhermore, as activated by Ox-mtDNA down-regulated, M2 phenotype anti-inflammatory factors increase. Collectively, CeO2–Y@ZIF-8@Gel, remodulating crosstalk macrophage reprogramming angiogenesis alleviate microenvironment accelerates

Language: Английский

Citations

25
Multifunctional PDA/ZIF8 based hydrogel dressing modulates the microenvironment to accelerate chronic wound healing by ROS scavenging and macrophage polarization DOI
Jinhong Cai, Shuhan Liu,

Qiuling Zhong

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 487, P. 150632 - 150632

Published: March 24, 2024

Language: Английский

Citations

24
Lactic acid promotes nucleus pulposus cell senescence and corresponding intervertebral disc degeneration via interacting with Akt DOI Open Access
Yuyao Zhang, Libangxi Liu, Yuhan Qi

et al.

Cellular and Molecular Life Sciences, Journal Year: 2024, Volume and Issue: 81(1)

Published: Jan. 12, 2024

Language: Английский

Citations

22
Effects of three Huanglian-derived polysaccharides on the gut microbiome and fecal metabolome of high-fat diet/streptozocin-induced type 2 diabetes mice DOI
Jiahao Wang,

Guangqin An,

Xian-Zhi Peng

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 273, P. 133060 - 133060

Published: June 12, 2024

Language: Английский

Citations

18
Theranostic hydrogels: Construction strategies and applications DOI

Xiaolong Li,

Ruiyang Li, Fuxiao Wang

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: 505, P. 159545 - 159545

Published: Jan. 13, 2025

Language: Английский

Citations

3
Angiogenesis during diabetic wound repair: from mechanism to therapy opportunity DOI Creative Commons
Kang Huang, Bobin Mi, Yuan Xiong

et al.

Burns & Trauma, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 1, 2025

Abstract Diabetes mellitus, a pervasive chronic metabolic disorder, is often associated with complications such as impaired wound healing. Various factors, most notably vascular deficiency, govern the repair process in diabetic patients, significantly impeding healing; therefore, angiogenesis and its role have emerged important areas of research. This review aims to delve into mechanisms angiogenesis, effects diabetes on association between repair. will ultimately offer valuable guidance regarding ideal timing treatment clinical setting.

Language: Английский

Citations

3
Selenomethionine in gelatin methacryloyl hydrogels: Modulating ferroptosis to attenuate skin aging DOI Creative Commons
Jiachen Sun,

Xiaoye Xie,

Yaoyao Song

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 35, P. 495 - 516

Published: Feb. 21, 2024

During skin aging, the degeneration of epidermal stem cells (EpiSCs) leads to diminished wound healing capabilities and disintegration. This study tackles this issue through a comprehensive analysis combining transcriptomics untargeted metabolomics, revealing age-dependent alterations in

Language: Английский

Citations

11
Microenvironmental dynamics of diabetic wounds and insights for hydrogel-based therapeutics DOI Creative Commons
Ying Zhao,

Yulan Zhao,

Bing Xu

et al.

Journal of Tissue Engineering, Journal Year: 2024, Volume and Issue: 15

Published: Jan. 1, 2024

The rising prevalence of diabetes has underscored concerns surrounding diabetic wounds and their potential to induce disability. intricate healing mechanisms are multifaceted, influenced by ambient microenvironment, including prolonged hyperglycemia, severe infection, inflammation, elevated levels reactive oxygen species (ROS), ischemia, impaired vascularization, altered wound physicochemical properties. In recent years, hydrogels have emerged as promising candidates for treatment owing exceptional biocompatibility resemblance the extracellular matrix (ECM) through a three-dimensional (3D) porous network. This review will first summarize microenvironment alterations occurring in wounds, aiming provide comprehensive understanding its pathogenesis, then classification recently developed be presented, encompassing properties such hypoglycemic effects, anti-inflammatory capabilities, antibacterial attributes, ROS scavenging abilities, promotion angiogenesis, pH responsiveness, more. primary objective is offer valuable reference repairing based on unique microenvironment. Moreover, this paper outlines avenues future advancements hydrogel dressings facilitate expedite process wounds.

Language: Английский

Citations

10
A novel diabetic foot ulcer diagnostic model: identification and analysis of genes related to glutamine metabolism and immune infiltration DOI Creative Commons
Hongshuo Shi, Xin Yuan, Xiao Yang

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Jan. 30, 2024

Abstract Background Diabetic foot ulcer (DFU) is one of the most common and severe complications diabetes, with vascular changes, neuropathy, infections being primary pathological mechanisms. Glutamine (Gln) metabolism has been found to play a crucial role in diabetes complications. This study aims identify validate potential Gln biomarkers associated DFU through bioinformatics machine learning analysis. Methods We downloaded two microarray datasets related patients from Gene Expression Omnibus (GEO) database, namely GSE134431, GSE68183, GSE80178. From GSE134431 dataset, we obtained differentially expressed Gln-metabolism genes (deGlnMRGs) between normal controls. analyzed correlation deGlnMRGs immune cell infiltration status. also explored relationship GlnMRGs molecular clusters Notably, WGCNA (DEGs) within specific clusters. Additionally, conducted GSVA annotate enriched genes. Subsequently, constructed screened best model. Finally, validated predictions' accuracy using nomogram, calibration curves, decision curve analysis (DCA), GSE80178 dataset. Results In both control groups, confirmed presence an activated response. 20 deGlnMRGs, including CTPS1, NAGS, SLC7A11, GGT1, GCLM, RIMKLA, ARG2, ASL, ASNS, ASNSD1, PPAT, GLS2, GLUD1, MECP2, ASS1, PRODH, CTPS2, ALDH5A1, DGLUCY, SLC25A12. Furthermore, were identified DFU. Immune indicated heterogeneity these established Support Vector Machine (SVM) model based on 5 (R3HCC1, ZNF562, MFN1, DRAM1, PTGDS), which exhibited excellent performance external validation datasetGSE134431, (AUC = 0.929). Conclusion five DFU, revealing novel therapeutic targets for immune-inflammatory cells plays progression

Language: Английский

Citations

9
Greasing the wheels of inflammasome formation: regulation of NLRP3 function by S-linked fatty acids DOI Creative Commons
Daniel M. Williams, Andrew A. Peden

Biochemical Society Transactions, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

NLRP3 is an inflammasome seeding pattern recognition receptor that initiates a pro-inflammatory signalling cascade in response to changes intracellular homeostasis are indicative of bacterial infection or tissue damage. Several types post-translational modification (PTM) have been identified added regulate its activity. Recent progress has revealed subject further type PTM, S-acylation (or palmitoylation), which involves the reversible addition long-chain fatty acids target cysteine residues by opposing sets enzymes. This review provides overview recent studies as important modifier function. The essential role recruitment membranes and consequences S-acylation-dependent membrane on localisation activation discussed detail.

Language: Английский

Citations

1