Emerging contaminants: A One Health perspective

The Innovation, Journal Year: 2024, Volume and Issue: 5(4), P. 100612 - 100612

Published: March 13, 2024

Language: Английский

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CuCo₂O₄ Nanoflowers with Multiple Enzyme Activities for Treating Bacterium-Infected Wounds via Cuproptosis-like Death

ACS Nano, Journal Year: 2024, Volume and Issue: 18(24), P. 15845 - 15863

Published: June 4, 2024

Nanozyme-driven catalytic therapy provides a promising treatment strategy for bacterial biofilm-infected wounds. However, the single functionality and limited efficiency of nanozyme-based materials often restrict effectiveness wound infection treatment. In this study, CuCo2O4 nanoflowers with multiple enzymatic activities were prepared antibacterial/antibiofilm by cuproptosis-like death. exhibited peroxidase-like (POD-like) oxidase-like (OXD-like) dual enzyme that generated large amounts •OH O2•–. Moreover, glutathione (GSH-Px-like) activity was able to reduce overexpression GSH in microenvironment, enhancing therapeutic effects reactive oxygen species (ROS). The morphology modified using hydrothermal method PEG4000 as solvent, resulting exposure more active center sites significant improvement activity. vitro results demonstrated pronounced disruption effect on biofilms formed bacteria. vivo, significantly promoted angiogenesis, collagen deposition, cell proliferation. Transcriptome sequencing revealed elevated ROS levels bacteria led membrane damage metabolic disruption. addition, Cu2+ overload induces lipid peroxidation accumulation disrupts respiratory chain tricarboxylic acid (TCA) cycle, ultimately leading This strategy, which combines synergistic enzyme-like death, an approach treating biofilm infections.

Language: Английский

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Direct Z‐Scheme Xylan‐Based Carbon Dots@TiO_2‐x Nanocomposites for Visible Light Driven Photocatalytic of Dye Degradation and Antibacterial

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(32)

Published: May 13, 2024

Abstract TiO 2 photocatalysis has gained attention as a cost‐effective way of degrading contaminants. However, it relies on UV excitation and experiences rapid carrier recombination. To address these drawbacks, novel xylan‐based carbon dots@hydrogenated (CDs@TiO 2‐x ) composited by direct Z‐scheme heterojunction is fabricated visible light‐driven photocatalytic material, which broadens the range light absorption to suppresses recombination photogenerated carriers. The spindle‐shaped CDs@TiO C/N/O/S groups, positive charges, oxygen vacancies its surface, can degrade 89% methylene blue 87% methyl orange within 90 min, 99% rhodamine B 60 min in light. It also eradicates 99.9% E. coli h 97.1% S. aureus 2.5 h. These performances surpass CDs , exhibit advantages similar composite materials. degradation pathways dyes specific antibacterial processes induced ROS are thoroughly investigated through LC‐MS testing related enzyme activity assays. DFT calculations study role vacancies, mechanism, catalytic sites. This developed nanocomposite with applications both dye sterilization, offering insights for multifunctional materials based heterojunction.

Language: Английский

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Prospects and challenges for the application of tissue engineering technologies in the treatment of bone infections

Bone Research, Journal Year: 2024, Volume and Issue: 12(1)

Published: May 14, 2024

Osteomyelitis is a devastating disease caused by microbial infection in deep bone tissue. Its high recurrence rate and impaired restoration of deficiencies are major challenges treatment. Microbes have evolved numerous mechanisms to effectively evade host intrinsic adaptive immune attacks persistently localize the host, such as drug-resistant bacteria, biofilms, persister cells, intracellular small colony variants (SCVs). Moreover, microbial-mediated dysregulation microenvironment impedes regeneration process, leading defect repair. Despite advances surgical strategies drug applications for treatment infections within last decade, remain clinical management. The development application tissue engineering materials provided new infections, but comprehensive review their research progress lacking. This discusses critical pathogenic microbes skeletal system immunomodulatory effects on regeneration, highlights prospects technologies infections. It will inform translation antimicrobial repair management

Language: Английский

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Bioactive mesoporous silica nanoparticle-functionalized titanium implants with controllable antimicrobial peptide release potentiate the regulation of inflammation and osseointegration

Biomaterials, Journal Year: 2024, Volume and Issue: 305, P. 122465 - 122465

Published: Jan. 2, 2024

Language: Английский

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Dual-sided centripetal microgrooved poly (D,L-lactide-co-caprolactone) disk encased in immune-regulating hydrogels for enhanced bone regeneration

Materials Today Bio, Journal Year: 2025, Volume and Issue: 30, P. 101436 - 101436

Published: Jan. 5, 2025

Well-designed artificial scaffolds are urgently needed due to the limited self-repair capacity of bone, which hampers effective regeneration in critical defects. Optimal must provide physical guidance recruit cells and immune regulation improve regenerative microenvironment. This study presents a novel scaffold composed dual-sided centripetal microgrooved poly(D,L-lactide-co-caprolactone) (PLCL) film combined with dynamic hydrogel containing prednisolone (PLS)-loaded Prussian blue nanoparticles (PB@PLS). The microgrooves on surface PLCL were imprinted using micropatterned polydimethylsiloxane (PDMS) template. Following aminolysis, was covalently grafted EM-7 peptide via glutaraldehyde. Functional group analysis, morphology hydrophilicity evaluated X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), an optical contact angle measuring instrument, respectively. Bone regeneration-related (e.g., bone marrow mesenchymal stem cells, macrophages, Schwann endothelial cells) cultured films tended align along stripes migrate from periphery toward center region vitro. Subsequently, encapsulated immune-regulating synthesized thiol-modified gelatin Cu2+ presence PB@PLS nanoparticles, demonstrated excellent antioxidant properties. significantly accelerated critical-sized regeneration, as evidenced by increase volume newly formed histological images vivo. innovative approach holds substantial promise for clinical applications broader tissue repair.

Language: Английский

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Bioinspired super-hydrophilic zwitterionic polymer armor combats thrombosis and infection of vascular catheters

Bioactive Materials, Journal Year: 2024, Volume and Issue: 37, P. 493 - 504

Published: April 25, 2024

Thrombosis and infection are two major complications associated with central venous catheters (CVCs), which significantly contribute to morbidity mortality. Antifouling coating strategies currently represent an efficient approach for addressing such complications. However, existing antifouling coatings have limitations in terms of both duration effectiveness. Herein, we propose a durable zwitterionic polymer armor catheters. This is realized by pre-coating robust phenol-polyamine film inspired insect sclerotization, followed grafting poly-2-methacryloyloxyethyl phosphorylcholine (pMPC) via

Language: Английский

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Zinc finger-inspired peptide-metal-phenolic nanointerface enhances bone-implant integration under bacterial infection microenvironment through immune modulation and osteogenesis promotion

Bioactive Materials, Journal Year: 2024, Volume and Issue: 41, P. 564 - 576

Published: Aug. 24, 2024

Orthopedic and dental implantations under bacterial infection microenvironment face significant challenges in achieving high-quality bone-implant integration. Designing implant coatings that incorporate both immune defense anti-inflammation is difficult conventional single-functional coatings. We introduce a multifunctional nanointerface using zinc finger-inspired peptide-metal-phenolic nanocoating, designed to enhance osseointegration such conditions. Abaloparatide (ABL), second-generation anabolic drug for treating osteoporosis, can be integrated into the design of zinc-phenolic network constructed on surface (ABL@ZnTA). Importantly, phenolic-coordinated Zn

Language: Английский

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A new therapeutic strategy for infectious diseases against intracellular multidrug-resistant bacteria

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 375, P. 467 - 477

Published: Sept. 20, 2024

Bacterial infections result in 7,700,000 deaths per year globally, with intracellular bacteria causing repeated and resistant infection. No drug is currently licenced for the treatment of bacteria. A new screening platform mimicking host milieu has been established to explore phytochemical antibiotic adjuvants. Previously neglected isoprenylated flavonoids were found be effective against methicillin-resistant Staphylococcus aureus (MRSA) vancomycin-resistant enterococci (VRE). Specifically, synergistic effect between glabrol streptomycin was observed first time. The glabrol-streptomycin combination targets bacterial inner membrane phospholipids, disrupts arginine biosynthesis, inhibits cell wall proteins biofilm formation genes (agrA/B/C/D), promotes ROS production, subsequent damage. To enhance selective uptake into infected cells, hyaluronic acid-streptomycin-lipoic acid-glabrol nanoparticles (HSLGS-S) designed synthesized trigger delivery combination. Thus, can transported region selectively release at site. bioactivity HSLGS-S clearing 20-fold higher than that alone vitro 2- 10-fold vivo.

Language: Английский

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Surface Chemistry Engineered Selenium Nanoparticles as Bactericidal and Immuno-modulating Dual-functional Agents for Combating Methicillin-resistant Staphylococcus aureus Infection

Drug Resistance Updates, Journal Year: 2024, Volume and Issue: 76, P. 101102 - 101102

Published: June 15, 2024

Language: Английский

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