Single-cell ATAC sequencing illuminates the cis-regulatory differentiation of taxol biosynthesis between leaf mesophyll and leaf epidermal cells in Taxus mairei DOI

Xiaori Zhan,

Hongshan Zhang,

Xueshuang Liang

et al.

Industrial Crops and Products, Journal Year: 2023, Volume and Issue: 205, P. 117411 - 117411

Published: Sept. 14, 2023

Language: Английский

Single-cell RNA sequencing facilitates the elucidation of the complete biosynthesis of the antidepressant hyperforin in St. John’s wort DOI
Song Wu, Ana Luísa Malaco Morotti, Jun Yang

et al.

Molecular Plant, Journal Year: 2024, Volume and Issue: 17(9), P. 1439 - 1457

Published: Aug. 12, 2024

Language: Английский

Citations

9
From Taxus to paclitaxel: Opportunities and challenges for urban agriculture to promote human health DOI Creative Commons

Xiulan Xie,

Yaohua Zhai,

Hao Cheng

et al.

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: 220, P. 109502 - 109502

Published: Jan. 12, 2025

Language: Английский

Citations

1
Correlation Analysis of Secondary Metabolism and Endophytic Fungal Assembles Provide Insights Into Screening Efficient Taxol‐Related Fungal Elicitors DOI Creative Commons
Mingshuang Wang,

Xueshuang Liang,

Ruoyun Ma

et al.

Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

ABSTRACT The efficacy of Taxol, a natural anticancer drug, in the treatment various types cancers has been certified globally. Fungal elicitors have reported as an impressive strategy for enhancing Taxol biosynthesis. We investigated effect twig age on biosynthesis and communities endophytic fungi. A negative correlation between content complexity fungal community twigs was predicted. Endogenous taxoids, similar to balancing valves, might specific controlling microbiota assembly Taxus twigs. Utilising special correlation, 11 isolates age‐associated fungi were used screen new involved Two efficient elicitors, L01 ( Guignardia ) J02 Diaporthe ), identified, increasing contents by 5.91‐ 4.83‐folds, respectively. It is confirmed that effective may be negatively correlated with tissues. Furthermore, significantly induced jasmonic acid (JA) content, speculating involvement MYC2a‐controlled JA signalling elicitor‐activated Our data revealed provided novel approach

Language: Английский

Citations

1
Imaging plant metabolism in situ DOI Creative Commons
Patrick J. Horn, Kent D. Chapman

Journal of Experimental Botany, Journal Year: 2023, Volume and Issue: 75(6), P. 1654 - 1670

Published: Oct. 27, 2023

Mass spectrometry imaging (MSI) has emerged as an invaluable analytical technique for investigating the spatial distribution of molecules within biological systems. In realm plant science, MSI is increasingly employed to explore metabolic processes across a wide array tissues, including those in leaves, fruits, stems, roots, and seeds, spanning various systems such model species, staple energy crops, medicinal plants. By generating maps metabolites, elucidated patterns diverse metabolites phytochemicals, encompassing lipids, carbohydrates, amino acids, organic phenolics, terpenes, alkaloids, vitamins, pigments, others, thereby providing insights into their pathways functional roles. this review, we present recent studies that demonstrate advances made visualizing metabolome. Moreover, emphasize technical progress enhances identification interpretation metabolite maps. Within mere decade since inception studies, robust technology poised continue vital tool tackling complex challenges metabolism.

Language: Английский

Citations

21
Functions of Representative Terpenoids and Their Biosynthesis Mechanisms in Medicinal Plants DOI Creative Commons
Qingjie Wang, Xiya Zhao,

Yang Jiang

et al.

Biomolecules, Journal Year: 2023, Volume and Issue: 13(12), P. 1725 - 1725

Published: Nov. 30, 2023

Terpenoids are the broadest and richest group of chemicals obtained from plants. These plant-derived terpenoids have been extensively utilized in various industries, including food pharmaceuticals. Several specific identified isolated medicinal plants, emphasizing diversity biosynthesis functionality terpenoids. With advances technology sequencing, genomes certain important plants assembled. This has improved our knowledge regulatory molecular functions with functions. In this review, we introduce several notable that produce distinct (e.g., Cannabis sativa, Artemisia annua, Salvia miltiorrhiza, Ginkgo biloba, Taxus media). We summarize specialized roles these plant-environment interactions as well their significance pharmaceutical industries. Additionally, highlight recent findings fields regulation mechanisms involved biosynthesis, propose future opportunities terpenoid research, biology seeding, genetic engineering

Language: Английский

Citations

19
Mass Spectrometry-Imaging Analysis of Active Ingredients in the Leaves of Taxus cuspidata DOI Creative Commons

Xiaori Zhan,

Yue Zang,

Ruoyun Ma

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(16), P. 18634 - 18642

Published: April 10, 2024

is an endangered evergreen conifer mainly found in Northeast Asia. In addition to the well-known taxanes, several active ingredients were detected leaves of

Language: Английский

Citations

5
Fungal endophytes of Taxus species and regulatory effect of two strains on taxol synthesis DOI Creative Commons
Hongshan Zhang, Wan-Ting Lin,

Ruoyun Ma

et al.

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Aug. 3, 2024

Taxol, derived from Taxus trees, is a valuable natural resource for the development of anticancer drugs. Endophytic fungi trees are promising alternative source Taxol. However, impact plant-endophytic microbial interaction on host's Taxol biosynthesis largely unknown. In current study, diversity endophytic in three different species was analyzed using Internal Transcribed Spacer sequencing. A total 271 Operational Taxonomic Units (OTUs) were identified, grouping into 2 phyla, 8 classes, 16 orders, 19 families, and genera. Alpha beta analysis indicated significant differences fungal communities among various trees. At genus level, Alternaria Davidiella predominantly found T. mairei media, respectively. By utilizing previously published dataset, Pearson correlation conducted to predict taxol biosynthesis-related Following screening, two isolates (L7 M14) obtained. Effect inoculation with gene expression metabolite accumulation determined by transcriptomic untargeted metabolomic studies. The co-inoculation assay suggests that may have negative regulatory effect influencing hormone signaling pathways. Our findings will serve as foundation advancing production utilization also aid screening related production.

Language: Английский

Citations

4
An overview of single-cell high-throughput technology in plants DOI
Lucas Auroux, Lim Chee Liew, Mathew G. Lewsey

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 34

Published: Jan. 1, 2025

Language: Английский

Citations

0
Visualization and Spatial Mapping of PFAS in the Edible Storage Root of Radish DOI Creative Commons

Yuwei Zuo,

Weilan Zhang

Journal of Hazardous Materials Letters, Journal Year: 2025, Volume and Issue: 6, P. 100141 - 100141

Published: Feb. 1, 2025

Language: Английский

Citations

0
Single-cell RNA sequencing reveals transcriptional regulation and metabolic pathways of terpenoid biosynthesis in developing Cinnamomum camphora leaf cells DOI Creative Commons
Zheng Qin,

Caihui Chen,

Ting Zhang

et al.

Current Plant Biology, Journal Year: 2025, Volume and Issue: unknown, P. 100467 - 100467

Published: March 1, 2025

Language: Английский

Citations

0