Single-cell ATAC sequencing illuminates the cis-regulatory differentiation of taxol biosynthesis between leaf mesophyll and leaf epidermal cells in Taxus mairei DOI

Xiaori Zhan,

Hongshan Zhang,

Xueshuang Liang

et al.

Industrial Crops and Products, Journal Year: 2023, Volume and Issue: 205, P. 117411 - 117411

Published: Sept. 14, 2023

Language: Английский

Recent progress in single-cell transcriptomic studies in plants DOI Creative Commons
Yuhan Cho, Ulhas Sopanrao Kadam,

Bogun Park

et al.

Plant Biotechnology Reports, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Language: Английский

Citations

0
Role of an endodermis‐specific miR858b‐MYB1L module in the regulation of Taxol biosynthesis in Taxus mairei DOI

Chunna Yu,

D. Zhang,

Lingxiao Zhang

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 122(1)

Published: April 1, 2025

SUMMARY Taxol, a chemotherapeutic agent widely used for treating various cancers, is extracted from the stems of Taxus mairei . However, current knowledge regarding effects stem tissue and age on Taxol accumulation limited. We employed matrix‐assisted laser desorption/ionization mass spectrometry to visualize taxoids in section sections varying ages T. Laser capture microdissection integrated with data‐dependent acquisition–MS/MS analysis identified that several biosynthesis pathway‐related enzymes were predominantly produced endodermis, elucidating molecular mechanisms underlying endodermis‐specific accumulation. an MYB1‐like (MYB1L) protein proposed potential function miR858‐MYB1L module regulating secondary metabolic pathways. DNA affinity purification sequencing 92 506 target peaks MYB1L. Motif enrichment de novo motifs, providing new insights into MYB recognition sites. Four MYB1L within promoter sequences synthesis genes, including TBT , DBTNBT T13OH BAPT confirmed using electrophoretic mobility shift assays. Dual‐luciferase assays showed significantly activated expression Our data indicate miR858b‐MYB1L plays crucial role transcriptional regulation by up‐regulating genes endodermis.

Language: Английский

Citations

0
Spatial multi-omics in medicinal plants: from biosynthesis pathways to industrial applications DOI Creative Commons
Chen Chen, Xiao Zhang, Ming Yue

et al.

Trends in Plant Science, Journal Year: 2024, Volume and Issue: 29(5), P. 510 - 513

Published: March 13, 2024

With the rapid development of molecular sequencing and imaging technology, multi-omics medicinal plants enters single-cell era. We discuss spatial applied in plants, evaluate special products' biosynthesis pathways, highlight applications, perspectives, challenges biomanufacturing natural products (NPs).

Language: Английский

Citations

3
Cell type specific regulation of phenolic acid and flavonoid metabolism in Taxus mairei leaves DOI

Xiaori Zhan,

Xueshuang Liang,

Wan-Ting Lin

et al.

Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 219, P. 118975 - 118975

Published: June 24, 2024

Language: Английский

Citations

3
Sex-specific responses of Taxus mairei to UV-B radiation involved altering the interactions between the microbiota assembly and host secondary metabolism DOI Creative Commons
Hongshan Zhang,

Kailin Hou,

Xueshuang Liang

et al.

Microbiome, Journal Year: 2024, Volume and Issue: 12(1)

Published: Sept. 7, 2024

Language: Английский

Citations

3
Advances in Single-Cell Transcriptome Sequencing and Spatial Transcriptome Sequencing in Plants DOI Creative Commons

Zhuo Lv,

Shuaijun Jiang,

Shuxin Kong

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(12), P. 1679 - 1679

Published: June 18, 2024

“Omics” typically involves exploration of the structure and function entire composition a biological system at specific level using high-throughput analytical methods to probe analyze large amounts data, including genomics, transcriptomics, proteomics, metabolomics, among other types. Genomics characterizes quantifies all genes an organism collectively, studying their interrelationships impacts on organism. However, conventional transcriptomic sequencing techniques target population cells, results only reflect average expression levels in as they are unable reveal gene heterogeneity spatial individual thus masking specificity between different cells. Single-cell transcriptome cells plant or animal tissues, enabling understanding each cell’s metabolites expressed genes. Consequently, statistical analysis corresponding tissues can be performed, with purpose achieving cell classification, evolutionary growth, physiological pathological analyses. This article provides overview research progress single-cell well applications challenges plants. Furthermore, prospects for development transcriptomics proposed.

Language: Английский

Citations

2
Genome-wide identification of AP2/ERF gene family in Coptis Chinensis Franch reveals its role in tissue-specific accumulation of benzylisoquinoline alkaloids DOI Creative Commons
Mengyu Zhang,

Pingping Lu,

Yating Zheng

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Oct. 16, 2024

The Plant-specific AP2/ERF gene family encodes proteins involved in various biological and physiological processes. Although the genome of Coptis chinensis Franch, a plant producing benzylisoquinoline alkaloids (BIAs), has been sequenced at chromosome level, studies on C. are lacking. Thus, genome-wide identification was conducted to explore its role BIAs biosynthesis.

Language: Английский

Citations

2
Post-genomic illumination of paclitaxel biosynthesis DOI
Alisdair R. Fernie, Fang Liu, Youjun Zhang

et al.

Nature Plants, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 27, 2024

Language: Английский

Citations

2
Comprehensive Strategies for Paclitaxel Production: Insights from Plant Cell Culture, Endophytic Microorganisms, and Synthetic Biology DOI Creative Commons
Junfa Yin, Meng Lai, Xiaoying Yu

et al.

Horticulture Research, Journal Year: 2024, Volume and Issue: 12(3)

Published: Dec. 12, 2024

Abstract Taxus L., an important ornamental, economic, and medicinal plant, is renowned for producing paclitaxel (Taxol®), a potent chemotherapeutic agent. The biosynthesis of involves intricate biosynthetic pathways, spanning multiple enzymatic steps. Despite advances, challenges remain in optimizing production methods. Microorganisms, particularly endophytic fungi, show potential paclitaxel, though with limitations yield stability. suspension culture cells promising alternative, offering sustainable production, yet it requires further genetic environmental optimization. Recent advancements synthetic biology have enabled partial reconstitution pathways microbial plant chassis. However, achieving complete remains ongoing challenge. This review consolidates recent progress biosynthesis, highlighting current future prospects industrial-scale production.

Language: Английский

Citations

2
Application and prospects of single-cell and spatial omics technologies in woody plants DOI Creative Commons

Shaoming Liang,

Yiling Li, Yang Chen

et al.

Forestry Research, Journal Year: 2023, Volume and Issue: 3(1), P. 0 - 0

Published: Jan. 1, 2023

Over the past decade, high-throughput sequencing and high-resolution single-cell transcriptome technologies have undergone rapid development, leading to significant breakthroughs. Traditional molecular biology methods are limited in their ability unravel cellular-level heterogeneity within woody plant tissues. Consequently, techniques such as transcriptomics, epigenetics, spatial transcriptomics rapidly gaining popularity study of plants. In this review, we provide a comprehensive overview development these technologies, with focus on applications challenges they present research particular, delve into similarities differences among results current studies analyze reasons behind differences. Furthermore, put forth potential solutions overcome encountered Finally, discuss application directions address key future.

Language: Английский

Citations

4