The U-box E3 ubiquitin ligase PUB35 negatively regulates ABA signaling through AFP1-mediated degradation of ABI5

The Plant Cell, Journal Year: 2024, Volume and Issue: 36(9), P. 3277 - 3297

Published: June 26, 2024

Abstract Abscisic acid (ABA) signaling is crucial for plant responses to various abiotic stresses. The Arabidopsis (Arabidopsis thaliana) transcription factor ABA INSENSITIVE 5 (ABI5) a central regulator of signaling. ABI5 BINDING PROTEIN 1 (AFP1) interacts with and facilitates its 26S-proteasome-mediated degradation, although the detailed mechanism has remained unclear. Here, we report that an ABA-responsive U-box E3 ubiquitin ligase, PLANT U-BOX 35 (PUB35), physically AFP1 ABI5. PUB35 directly ubiquitinated in bacterially reconstituted ubiquitination system promoted protein degradation vivo. was enhanced by response treatment. Phosphorylation T201 T206 residues disrupted ABI5–AFP1 interaction affected ABI5–PUB35 PUB35-mediated Genetic analysis seed germination seedling growth showed pub35 mutants were hypersensitive as well salinity osmotic stresses, whereas overexpression lines hyposensitive. Moreover, abi5 epistatic pub35, pub35-2 afp1-1 double mutant similar two single mutants. Together, our results reveal PUB35–AFP1 module involved fine-tuning through during growth.

Language: Английский

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Mechanisms of Abscisic Acid-mediated Plant Defense Responses: An Updated Review

Plant Stress, Journal Year: 2024, Volume and Issue: unknown, P. 100724 - 100724

Published: Dec. 1, 2024

Language: Английский

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Elucidating the role of exogenous melatonin in mitigating alkaline stress in soybeans across different growth stages: a transcriptomic and metabolomic approach

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: May 8, 2024

Abstract Background Soybean ( Glycine max ), a vital grain and oilseed crop, serves as primary source of plant protein oil. Soil salinization poses significant threat to soybean planting, highlighting the urgency improve resilience adaptability saline stress. Melatonin, recently identified key growth regulator, plays crucial roles in growth, development, responses environmental However, potential melatonin mitigate alkali stress soybeans underlying mechanisms remain unclear. Results This study investigated effects exogenous on cultivar Zhonghuang 13 under alkaline We employed physiological, biochemical, transcriptomic, metabolomic analyses throughout both vegetative pod-filling stages. Our findings demonstrate that significantly counteracts detrimental plants, promoting photosynthesis, antioxidant capacity. Transcriptomic analysis during stages stress, with without treatment, 2,834 549 differentially expressed genes, respectively. These genes may play role regulating adaptation abiotic Notably, phytohormone biosynthesis pathways revealed altered expression particularly ARF (auxin response factor), AUX/IAA (auxin/indole-3-acetic acid), GH3 (Gretchen Hagen 3) families, early response. Metabolomic stage highly metabolites responding application, such uteolin-7-O-(2''-O-rhamnosyl)rutinoside Hederagenin-3-O-glucuronide-28-O-glucosyl(1,2)glucoside, which helped alleviate damage caused by Furthermore, we 183 transcription factors, potentially playing critical Among these, gene SoyZH13_04G073701 is noteworthy it regulates metabolite, terpene metabolite Hederagenin-3-O-glucuronide-28-O-glucosyl(1,2)glucoside. WGCNA this ) hub gene, positively terpenoids, provide novel insights into how alleviates at different reproductive Conclusions Integrating transcriptomic approaches, our elucidates ameliorates inhibitory development. occurs through modulation for compounds, including terpenes, flavonoids, phenolics. initial mechanistic mitigates soybeans, offering foundation molecular breeding strategies enhance salt-alkali tolerance crop.

Language: Английский

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Transcription factor OsbZIP10 modulates rice grain quality by regulating OsGIF1

The Plant Journal, Journal Year: 2024, Volume and Issue: 119(5), P. 2181 - 2198

Published: July 9, 2024

SUMMARY Understanding and optimizing the process of grain filling helps quest to maximize rice ( Oryza sativa L.) seed yield quality, yet intricate mechanisms at play remain fragmented. Transcription factors (TFs) are major players in gene networks underlying process. Here, we employed incomplete OsGIF1 )/ cell wall invertase 2 , a key involved filling, explore its upstream TFs identified bZIP family TF, OsbZIP10, be transcriptional activator . Rice grains knockouts OsbZIP10 showed increased white‐core rates but lower amylose content (AC), leading better eating cooking qualities all genetic backgrounds investigated, though impact mutations on weight depended background. Multi‐omics analyses suggested that, addition multiple genes different biological processes contributing were targeted by including OsAGPS1 encoding ADP‐Glc pyrophosphorylase (AGPase) small subunit, homeostasis reactive oxygen species. Distinct make‐up was observed between japonica indica varieties, with majority varieties each subspecies belonging two haplotypes that closely associated AC. Overexpressing haplotype linked high‐AC low‐AC background Overall, this study sheds crucial light significance OsbZIP10–OsGIF1 module determination offering potential avenue for engineering produce seeds tailored attributes.

Language: Английский

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Exploring ABI5 regulation: Post‐translational control and cofactor interactions in ABA signaling

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(3)

Published: Feb. 1, 2025

SUMMARY Abscisic acid insensitive 5 (ABI5) is a pivotal transcription factor in abscisic (ABA) signaling, playing an essential role plant growth and responses to abiotic stress. This key regulator subject multifaceted regulation, especially on post‐translational mechanisms. Recent research has shed light the regulation of ABI5, encompassing both modifications (PTMs) modulation its transcriptional activity. In this review, we provide comprehensive overview current knowledge surrounding along with influence various cofactors activity protein stability. The potential biological roles PTMs ABI5 context ABA signaling stress are also explored. As one most extensively studied proteins environmental responses, sophisticated precise understanding regulatory mechanisms that govern not only beneficial for application genetic engineering but helpful our exploration fundamental principles regulation.

Language: Английский

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A tRNA‐Arg‐Derived Fragment Mediates Gene Silencing of the Development‐Related OsIIP5 in Rice Under Oxidative Stress

Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

ABSTRACT Stress leads to reactive oxygen species (ROS) generation in plants, which causes oxidative stress and DNA damage. Studies have demonstrated that rapid increases tRNA degradation. However, the function of tRNA‐derived small RNAs (tsRNA) plants under has seldom been evaluated. This study investigated short tsRNAs called fragments (tRFs) rice roots treated with hydrogen peroxide (H 2 O ). Small RNA sequencing RT‐qPCR revealed expression levels tRF‐ArgTCG‐5, comprising 19 nt, seedling H treatment. Degradome data indicated tRF‐ArgTCG‐5 bound 3’ untranslated region OsIIP5 transcript cleaved it at noncanonical sites. Agrobacterium transient assay tobacco leaves inhibited GFP fused binding site , revealing a post‐transcriptional regulatory relationship between . ABA treatment nitrogen deficiency played roles regulation expression. In addition, seedlings mutation exhibited low spikelet fertility. Conclusively, led accumulation accumulated its target may contribute grain yield.

Language: Английский

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Overexpression of the Transcription Factor GmbZIP60 Increases Salt and Drought Tolerance in Soybean (Glycine max)

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3455 - 3455

Published: April 7, 2025

The regulation of downstream responsive genes by transcription factors (TFs) is a critical step in the stress response system plants. While bZIP are known to play important roles reactions, their functional characterization soybeans remains limited. Here, we identified soybean gene, GmbZIP60, which encodes protein containing typical domain with basic region and leucine zipper region. Subcellular localization studies confirmed that GmbZIP60 localized nucleus. Expression analysis demonstrated induced salt stress, drought various plant hormone treatments, including abscisic acid (ABA), ethylene (ETH), methyl jasmonate (MeJA). Overexpressing (OE-GmbZIP60) transgenic rice enhanced tolerance both stresses. Quantitative real-time polymerase chain reaction (qRT-PCR) indicated expression levels abiotic stress-responsive were significantly higher plants than wild-type (WT) under conditions. Chromatin immunoprecipitation-qPCR (ChIP-qPCR) further directly binds promoters stress-related ABA, ETH, JA, salicylic (SA). Overall, these findings revealed as positive regulator tolerance.

Language: Английский

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OsAAH confers salt tolerance in rice seedlings

The Plant Journal, Journal Year: 2024, Volume and Issue: 120(5), P. 1954 - 1968

Published: Oct. 22, 2024

SUMMARY Soil salinization is becoming a great threat that reduces crop productivity worldwide. In this study, we found rice allantoate amidohydrolase ( OsAAH ) expression was significantly upregulated by salt stress, and its overexpression conferred tolerance at the seedling stage. Compared to wild type (WT), contents of ureides (allantoin allantoate) were increased in Osaah mutants reduced lines both before after treatments. Exogenous allantoin promoted overexpression, but not mutants. Subcellular localization showed also localized peroxisomes addition previously reported endoplasmic reticulum (ER). The differential peroxisome‐related genes identified between WT. Furthermore, H 2 O malondialdehyde (MDA) accumulated lines. activities antioxidant enzymes enhanced under NaCl treatment. transcription factor OsABI5 could directly bind promoter activate expression. Our findings reveal be induced stress through activation then confer enhancing scavenging capacity reactive oxygen species (ROS), which contributes breeding tolerance.

Language: Английский

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Melatonin Mediates Trade–Off in Antioxidant Responses and Nitrogen Metabolism and Benefits the Growth of Fragrant Rice Seedlings Under Light–Nitrogen Levels

Journal of soil science and plant nutrition, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 28, 2024

Language: Английский

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ABA and Melatonin: Players on the Same Field?

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12266 - 12266

Published: Nov. 15, 2024

In plants, abscisic acid (ABA) and melatonin (MT) are conventionally treated as molecules mitigating stress responses. To understand the mechanisms of ABA–MT interplay, we examined effects ABA MT treatment in loss-of-function mutants Arabidopsis thaliana exposed to high light (HL) stress. constantly suppressed ASMT encoding N-acetylserotonin methyltransferase context differential responses other biosynthesis genes both wild type (WT) mutants. However, this response was absent mutant with disrupted ABI4. Given that promoter region contains several potential ABI4-binding elements, these data suggest can be a target gene for A role ABI4 interactions between is supported by finding constitutively derepressed signaling cand2 gpa1, which exhibited elevated steady state levels transcripts were not regulated either or melatonin. addition, abi4 showed increased modulations expression catabolic M2H M3H treatment, inferring transcription factor negative regulator ABA-dependent changes content. Furthermore, all tested impaired synthesis displayed compared WT, while contributed downregulation key genes. Collectively, our results act antagonistically, modulating metabolism interactions, studied severe (SLS).

Language: Английский

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