Deciphering the catalytic mechanism of superoxide dismutase activity of carbon dot nanozyme

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Jan. 11, 2023

Nanozymes with superoxide dismutase (SOD)-like activity have attracted increasing interest due to their ability scavenge anion, the origin of most reactive oxygen species in vivo. However, SOD nanozymes reported thus far yet approach natural enzymes. Here, we report a carbon dot (C-dot) nanozyme catalytic over 10,000 U/mg, comparable that Through selected chemical modifications and theoretical calculations, show SOD-like C-dots relies on hydroxyl carboxyl groups for binding anions carbonyl conjugated π-system electron transfer. Moreover, C-dot exhibit intrinsic targeting oxidation-damaged cells effectively protect neuron ischemic stroke male mice model. Together, our study sheds light structure-activity relationship nanozymes, demonstrates potential treating oxidation stress related diseases.

Language: Английский

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Nanozymes: Definition, Activity, and Mechanisms

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(10)

Published: Feb. 17, 2023

Abstract “Nanozyme” is used to describe various catalysts from immobilized inorganic metal complexes, enzymes nanoparticles. Here, the history of nanozymes dvescribed in detail, and they can be largely separated into two types. Type 1 refer or on nanomaterials, which were dominant first decade since 2004. 2 nanozymes, rely surface catalytic properties are dominating type past decade. The definition evolving, a based same substrates products as able cover most currently claimed although may have different mechanisms compared their enzyme counterparts. A broader inspire application‐based research replace with nanomaterials for analytical, environmental, biomedical applications. Comparison also requires clear nanozyme unit. Four ways defining unit described, iron oxide horseradish peroxidase activity comparison examples each definition. Growing work devoted understanding mechanism provides basis further rational engineering active sites. Finally, future perspective field discussed.

Language: Английский

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Depletable peroxidase-like activity of Fe3O4 nanozymes accompanied with separate migration of electrons and iron ions

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Sept. 12, 2022

As pioneering Fe

Language: Английский

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Bioinspired copper single‐atom nanozyme as a superoxide dismutase‐like antioxidant for sepsis treatment

Exploration, Journal Year: 2022, Volume and Issue: 2(4)

Published: July 13, 2022

Sepsis is a systemic inflammatory response syndrome with high morbidity and mortality mediated by infection-caused oxidative stress. Early antioxidant intervention removing excessively produced reactive oxygen nitrogen species (RONS) beneficial to the prevention treatment of sepsis. However, traditional antioxidants have failed improve patient outcomes due insufficient activity sustainability. Herein, mimicking electronic structural characteristics natural Cu-only superoxide dismutase (SOD5), single-atom nanozyme (SAzyme) featuring coordinately unsaturated atomically dispersed Cu-N

Language: Английский

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Aggregation-Induced Emission (AIE), Life and Health

ACS Nano, Journal Year: 2023, Volume and Issue: 17(15), P. 14347 - 14405

Published: July 24, 2023

Light has profoundly impacted modern medicine and healthcare, with numerous luminescent agents imaging techniques currently being used to assess health treat diseases. As an emerging concept in luminescence, aggregation-induced emission (AIE) shown great potential biological applications due its advantages terms of brightness, biocompatibility, photostability, positive correlation concentration. This review provides a comprehensive summary AIE luminogens applied structure dynamic physiological processes, disease diagnosis treatment, detection monitoring specific analytes, followed by representative works. Discussions on critical issues perspectives future directions are also included. aims stimulate the interest researchers from different fields, including chemistry, biology, materials science, medicine, etc., thus promoting development fields life health.

Language: Английский

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Nanozymes: Versatile Platforms for Cancer Diagnosis and Therapy

Nano-Micro Letters, Journal Year: 2022, Volume and Issue: 14(1)

Published: April 6, 2022

Abstract Natural enzymes usually suffer from high production cost, ease of denaturation and inactivation, low yield, making them difficult to be broadly applicable. As an emerging type artificial enzyme, nanozymes that combine the characteristics nanomaterials are promising alternatives. On one hand, have enzyme-like catalytic activities regulate biochemical reactions. other also inherit properties nanomaterials, which can ameliorate shortcomings natural serve as versatile platforms for diverse applications. In this review, various mimic activity different introduced. The achievements in cancer diagnosis treatment technologies summarized by highlighting advantages these Finally, future research directions rapidly developing field outlooked."Image missing"

Language: Английский

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Single-atom nanozymes catalytically surpassing naturally occurring enzymes as sustained stitching for brain trauma

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Aug. 12, 2022

Regenerable nanozymes with high catalytic stability and sustainability are promising substitutes for naturally-occurring enzymes but limited by insufficient non-selective activities. Herein, we developed single-atom of RhN

Language: Английский

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Edge‐Site Engineering of Defective Fe–N₄ Nanozymes with Boosted Catalase‐Like Performance for Retinal Vasculopathies

Advanced Materials, Journal Year: 2022, Volume and Issue: 34(39)

Published: Aug. 12, 2022

Extensive efforts are devoted to refining metal sites for optimizing the catalytic performance of single-atom nanozymes (SANzymes), while contribution defect environment neighboring lacks attention. Herein, an iron-based SANzyme (Fe-SANzyme) is rationally designed by edge-site engineering, which intensively exposes edge-hosted defective Fe-N4 atomic anchored in hierarchical mesoporous structures. The Fe-SANzyme exhibits excellent catalase-like activity capable efficiently catalyzing decomposition H2 O2 into and O, with a kinetic KM value superior that natural catalase reported nanozymes. mechanistic studies depict defects introduce notable charge transfer from Fe atom carbon matrix, making central more activated strengthen interaction weaken OO bond. By performing catalysis, significantly scavenges reactive oxygen species (ROS) alleviates oxidative stress, thus eliminating pathological angiogenesis animal models retinal vasculopathies without affecting repair normal vessels. This work provides new way refine SANzymes engineering geometric structure around sites, demonstrates potential therapeutic effects nanozyme on vasculopathies.

Language: Английский

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Biosystem‐Inspired Engineering of Nanozymes for Biomedical Applications

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(10)

Published: Jan. 9, 2023

Abstract Nanozymes with intrinsic enzyme‐mimicking activities have shown great potential to become surrogates of natural enzymes in many fields by virtue their advantages high catalytic stability, ease functionalization, and low cost. However, due the lack predictable descriptors, most nanozymes reported past been obtained mainly through trial‐and‐error strategies, efficacy, substrate specificity, as well practical application effect under physiological conditions, are far inferior that enzymes. To optimize efficacies functions biomedical settings, recent studies introduced biosystem‐inspired strategies into nanozyme design. In this review, advances engineering leveraging refined structure enzymes, simulating behavior changes process, mimicking specific biological processes or living organisms, introduced. Furthermore, currently involved applications summarized. More importantly, current opportunities challenges design discussed. It is hoped based on bioinspired will be beneficial for constructing new generation broadening applications.

Language: Английский

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Unveiling the active sites on ferrihydrite with apparent catalase-like activity for potentiating radiotherapy

Nano Today, Journal Year: 2021, Volume and Issue: 41, P. 101317 - 101317

Published: Oct. 29, 2021

The use of catalase-like nanozymes in relieving tumor hypoxia has been a promising strategy for adjuvant radiotherapy, yet their catalytic mechanism and toxic potential remain elusive. While most studies on mechanisms focus the substrates products process, little is reported to analyze dominating surface structure activity nanozymes. Here, we ferrihydrite revealed its structure-activity relationship. Among ten main forms iron oxide nanomaterials, ferrihydrite, especially 2-line exhibited highest activity. Importantly, fitting density functional analysis that abundant iron-associated hydroxyl groups dominantly affect ferrihydrites. In addition, no peroxidase-like superoxide dismutase-like activities, constantly catalyzed decomposition H2O2 release O2 pH range 4.0–8.7. With help steady single physiological environment, safely effectively microenvironment in-situ produce significantly enhanced therapeutic effect radiotherapy. Moreover, excellent biosafety biodegradability. These findings demonstrate great as biocompatible nanozyme cancer therapy.

Language: Английский

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