DNA‐Templated Gold Nanoclusters for miRNA Diagnostics with Branched Hybridization Chain Reaction DOI Creative Commons
Hua Chai,

Yulin Zhu,

Peng Miao

et al.

Small Structures, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 17, 2024

In this study, a novel nonlinear branched hybridization chain reaction (BHCR) strategy is developed for accurate biosensing and bioimaging. Four fuel strands are designed. The first two assist the growth of primary long double‐stranded chain. Meanwhile, tails left, which consist trigger downstream HCR with second group strands. Branched DNA nanostructures thus formed, significantly amplify information initial target miRNA. Besides, output fluorescent signal originated from DNA‐templated gold nanoclusters (AuNCs). Surface ligands AuNCs always play important roles in regulation optical mechanisms. Herein, peptide/DNA‐stabilized prepared. Phosphorothioate‐modified (psDNA) used as template situ synthesis. short peptide (Tyr‐Cys‐Tyr, YCY) introduced protecting ligand, benefits significant Stokes shift via two‐step Förster resonance energy transfer. Coupling BHCR AuNCs, method performs satisfactory vitro intracellular It exhibits potential application investigation miRNA‐related bioprocesses.

Language: Английский

The roles of templates consisting of amino acids in the synthesis and application of gold nanoclusters DOI
Jinliang Ma, Mengmeng Yang, Bin Zhang

et al.

Nanoscale, Journal Year: 2024, Volume and Issue: 16(15), P. 7287 - 7306

Published: Jan. 1, 2024

This review builds general principles for directing the precise synthesis and intelligent application of gold nanoclusters (AuNCs) regulated by template consisting amino acids.

Language: Английский

Citations

15
Gold nanocluster-based fluorescent sensors forin vitroandin vivoratiometric imaging of biomolecules DOI

S. Santhoshkumar,

Manivannan Madhu, Wei‐Bin Tseng

et al.

Physical Chemistry Chemical Physics, Journal Year: 2023, Volume and Issue: 25(33), P. 21787 - 21801

Published: Jan. 1, 2023

Gold nanoclusters (AuNCs) are promising nanomaterials for ratiometric fluorescent probes due to their tunable fluorescence wavelengths dependent on size and structure, as well biocompatibility resistance photobleaching. By incorporating an additional spectral peak, dual-emission AuNC-based have been developed enhance the signal output reproducibility. These can be fabricated by integrating various luminescent with AuNCs. This review focuses preparation methods applications of derived from AuNCs other or dyes both in vitro vivo bioimaging target analytes. Additionally, delves into sensing mechanisms probes, synthetic strategies, challenges encountered when using bioimaging. Moreover, we explore application protein-stabilized thiolate-capped biosensing Two primary assembling fluorophores discussed: triggered assembly self-assembly. Finally, address issues associated propose future directions further advancing imaging agents.

Language: Английский

Citations

18
Supramolecular assembly-induced electrochemiluminescence enhancement of gold nanoclusters for hemoglobin detection DOI

Ge Xue,

Mingming Zhang, Fei Yin

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(5), P. 1355 - 1360

Published: Jan. 1, 2024

Gold nanoclusters (Au NCs) with excellent optical properties and biocompatibility have become one of the most promising electrochemiluminescence (ECL) emitters. However, low efficiency poor stability Au NCs restrict their applications in ECL. Herein, by supramolecular assembly L-arginine (Arg) 4-hydroxy-2-mercapto-6-methylpyrimidine (MTU) on surface NCs, Arg/MTU-Au enhanced ECL were prepared. Compared MTU-stabilized (MTU-Au NCs), increased 24.8 times. As a proof-of-concept, sensitive biosensing platform was constructed for detection hemoglobin (Hb) urine using as The proposed provides feasible strategy biomarkers has broad application prospects disease screening clinical marker detection.

Language: Английский

Citations

7
Iodine stimulated “in-situ” growth of His/GSH-AuNCs for the detection of iodine ions via ratiometric fluorescence DOI
Lingyang Zhang,

Yiying Wei,

Yü Huang

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 112717 - 112717

Published: Jan. 1, 2025

Language: Английский

Citations

1
Cyclodextrin-assisted fluorescein decoration of gold nanoclusters for ratiometric and selective Hg2+ analysis DOI Creative Commons
Siyuan Sun, Zhiqin Yuan

Frontiers in Analytical Science, Journal Year: 2025, Volume and Issue: 4

Published: Jan. 22, 2025

Introduction The construction of nanomaterial-based ratiometric detection systems usually requires covalent modification, which undergoes reactive environments and may change the natural properties supporting nanomaterials. Methods In this study, dualemissive fluorescein-decorated gold nanoclusters (F-Au NCs) through cyclodextrin-supported host–guest interaction (non-covalent modification) are explored as Hg 2+ nanoprobes d 10 –d -interactioncaused fluorescence quenching Au NCs. Results Discussion fluorescein decoration only provides a fluorescent internal reference but does not -Au NCs affinity nature. Ratiometric selective is achieved proposed F-Au NC probes with limit 15 nM. analysis in river water samples small relative standard deviations validates practical application these probes.

Language: Английский

Citations

0
Hydrogen-Bonded Organic Framework Nanozyme with Multi-Enzyme Activity for Chemiluminescence Sensing of Acetylcholinesterase and Screening Its Inhibitors DOI

Yunping Lai,

Shuangshuang He, Yuanying Chen

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 10, 2025

Strong and persistent chemiluminescence (CL) is essential for enhancing the detection accuracy reproducibility of CL-based analytical methods. In this study, we explored synergistic effects amino groups present on surface hydrogen-bonded organic framework (HOF-PyTTA, where PyTTA denoted as 4,4',4″,4‴-(pyrene-1,3,6,8-tetrayl)tetraaniline) materials N-(4-aminobutyl-N-ethylisoluminol) (ABEI) reducing gold nanoparticles (AuNPs) HOFs. Additionally, utilized substantial specific area abundant HOFs to sequester Co2+ ions, resulting in synthesis HAACo material. The exhibited remarkable peroxidase, oxidase, catalase mimetic activities, enabling luminol-H2O2 system maintain a glow-type CL phenomenon more than 1 h. Subsequently, developed point-of-care testing (POCT) sensor that integrated characteristics with smartphone technology 3D printing determination acetylcholinesterase (AChE) activity serum samples, well screening AChE inhibitors. demonstrated linear range from 0.001 40 mU mL-1, limit 0.00057 mL-1. calculated IC50 inhibitor tacrine was found be 21.9 nmol L-1, indicating good selectivity stability sensor. This work not only expands applications biosensing but also enriches utilization HOF chemistry, paving way development multifunctional HOF-based future applications.

Language: Английский

Citations

0
Development of a novel label-free fluorescence sensing system based on AuNPs@FIB-AuNCs for quantitative detection of plasmin in dairy products DOI

Mati Ullah Khan,

Yuyin Wu,

Jie Yang

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113824 - 113824

Published: May 1, 2025

Language: Английский

Citations

0
hROS‐Responsive Behavior for Long‐Term Stability of Cellulosic Gold Nanoclusters DOI
Shanshan Yu, Haoyuan Li,

Yujie Duan

et al.

Small, Journal Year: 2023, Volume and Issue: 20(13)

Published: Nov. 14, 2023

Abstract Understanding the gold core‐ligand interaction in nanoclusters (GNCs) is essential for on‐demand tailoring of their photoluminescence properties and long‐term stability. Here, inspired by suckers arranged directionally on tentacles octopus, a series GNCs with regulating ligand structures are grown stabilized cellulose nanocrystals (CNCs). The carboxylated CNCs providing an electron‐rich environment to promote luminescence stabilize it within 1 year through anchoring diluting effects, highest quantum yields reaches 31.02% ultrapure water. Interestingly, this bionic preparation strategy generally applicable various ligands hROS‐responsive nonresponsive construct tunable‐emission wavelength dual ratiometric probes. results show that designing specific structure inhibit transformation Au‐Au Au (I)‐ligand crucial regulate characteristics. As expected, interfacial compatible probe hROS limit detection 0.74 µmol L −1 can diagnose certain diseases intracellular imaging. This work provides important insights understanding during oxidation process triggered hROS.

Language: Английский

Citations

6
Rapid chemical reduction synthesis of copper nanoclusters with blue fluorescence for highly sensitive detection of furazolidone DOI
Yuyu Guo, Jiancheng Wang, Lili Zhang

et al.

Luminescence, Journal Year: 2024, Volume and Issue: 39(3)

Published: Feb. 28, 2024

Abstract Tannic acid (TA), as a stabilizing agent, was successfully utilized to establish blue‐emitting copper nanoclusters (TA‐Cu NCs) on the basis of facile chemical reduction preparation method. Characterization results proved successful synthesis TA‐Cu NCs with uniform size and excellent stability. exhibited blue emission wavelength at 431 nm when excited 364 nm. Interestingly, as‐prepared were selectively quenched by furazolidone based static quenching. In addition, this analysis platform for detection had an linear range from 0.5 120 μM limit 0.074 ( S/N = 3). Furthermore, accuracy sensing method confirmed detecting in bovine serum samples, indicating that bright application prospects.

Language: Английский

Citations

1
Engineering in situ growth of Au nanoclusters on hydrophilic paper fibres for fluorescence calligraphy-based chemical logic gates and information encryption DOI
Jun Jiang Luo,

Dun Ying Guo,

Zi Bo Qu

et al.

Nanoscale Horizons, Journal Year: 2024, Volume and Issue: 9(11), P. 2007 - 2015

Published: Jan. 1, 2024

Gold nanoclusters (AuNCs) are a type of rising-star fluorescence nanomaterials, but their properties and applications hindered by the multi-step synthesis purification routes, as well lack desired supporting substrates. To enhance optical performance working efficiency, AuNCs suggested to be merged with emerging Herein, glutathione-modified hydrophilic rice papers incubated in chloroauric acid aqueous solutions, oxidation-reduction reaction between glutathione Au ions enables situ formation fluorescent on solid fibres papers. The growth resulted eye-catching tracks, similar traditional Chinese conventional calligraphy; thus, this fluoresence calligraphy is defined work. entire process, including signal responses, extremely simple, rapid, repeatable. Moreover, diversity additive chemical reagents studied responsive calligraphy, as-synthesized AuNC materials exhibited high reliability stability. Significantly, integration synchronous application paper substrates, high-performance logical gates information encryption systems were constructed, remarkably facilitating progress molecular sensing important transmission.

Language: Английский

Citations

1