Angewandte Chemie,
Journal Year:
2023,
Volume and Issue:
135(46)
Published: Sept. 28, 2023
Abstract
To
realize
sensing
and
labeling
biomarkers
is
quite
challenging
in
terms
of
designing
multimodal
imaging
probes.
In
this
study,
we
developed
a
novel
β
‐galactosidase
(
‐gal)
activated
bimodal
probe
that
combines
near‐infrared
(NIR)
fluorescence
magnetic
resonance
(MRI)
to
enable
real‐time
visualization
activity
living
organisms.
Upon
‐gal
activation,
Gal‐Cy‐Gd‐1
exhibits
remarkable
42‐fold
increase
NIR
intensity
at
717
nm,
allowing
covalent
adjacent
target
enzymes
or
proteins
avoiding
molecular
escape
promote
accumulation
the
tumor
site.
This
reaction
enhances
longitudinal
relaxivity
by
approximately
1.9
times,
facilitating
high‐resolution
MRI.
The
unique
features
precise
live
cells
mice.
probe's
utilization
aids
identifying
situ
ovarian
tumors,
offering
valuable
assistance
removal
tissue
during
surgical
procedures
fusion
MRI
activation
through
self‐immobilizing
provides
robust
approach
for
visualizing
activity.
Moreover,
sets
groundwork
developing
other
activatable
probes,
vivo
enzyme
localization.
Journal of the American Chemical Society,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 4, 2025
Liver
disease
poses
a
significant
challenge
to
global
health,
and
its
early
diagnosis
is
crucial
for
improving
treatment
outcomes
patient
prognosis.
Since
fluctuation
of
key
biomarkers
during
the
onset
progression
liver
diseases
can
directly
reflect
health
normal/abnormal
function,
biomarker-based
assays
are
vital
tools
detection
disease.
In
this
context,
small
molecule
fluorescent
probes
have
undeniably
emerged
as
indispensable
analysis,
with
an
ever-growing
number
molecule-based
being
developed
over
recent
years,
sole
aim
monitoring
relevant
This
perspective
will
focus
on
development
application
primarily
last
10
years
diagnosing
range
disease-related
processes.
It
outline
foundational
design
strategies
developing
promising
probes,
their
optical
response
biomarkers,
how
they
been
demonstrated
in
proof-of-concept
imaging
applications.
Current
challenges
new
developments
field
be
discussed,
providing
insights
highlighting
opportunities
field.
Angewandte Chemie International Edition,
Journal Year:
2023,
Volume and Issue:
62(46)
Published: Sept. 28, 2023
Abstract
To
realize
sensing
and
labeling
biomarkers
is
quite
challenging
in
terms
of
designing
multimodal
imaging
probes.
In
this
study,
we
developed
a
novel
β
‐galactosidase
(
‐gal)
activated
bimodal
probe
that
combines
near‐infrared
(NIR)
fluorescence
magnetic
resonance
(MRI)
to
enable
real‐time
visualization
activity
living
organisms.
Upon
‐gal
activation,
Gal‐Cy‐Gd‐1
exhibits
remarkable
42‐fold
increase
NIR
intensity
at
717
nm,
allowing
covalent
adjacent
target
enzymes
or
proteins
avoiding
molecular
escape
promote
accumulation
the
tumor
site.
This
reaction
enhances
longitudinal
relaxivity
by
approximately
1.9
times,
facilitating
high‐resolution
MRI.
The
unique
features
precise
live
cells
mice.
probe's
utilization
aids
identifying
situ
ovarian
tumors,
offering
valuable
assistance
removal
tissue
during
surgical
procedures
fusion
MRI
activation
through
self‐immobilizing
provides
robust
approach
for
visualizing
activity.
Moreover,
sets
groundwork
developing
other
activatable
probes,
vivo
enzyme
localization.
Chemical & Biomedical Imaging,
Journal Year:
2023,
Volume and Issue:
1(6), P. 495 - 508
Published: June 1, 2023
Inflammation
plays
an
important
role
in
the
occurrence
and
development
of
disease;
dysregulation
inflammatory
progression
often
leads
to
disease
such
as
tissue
sclerosis,
cancers,
stroke,
etc.
Optical
imaging
technology,
due
its
higher
sensitivity
resolution,
can
provide
finer
images
for
observation
inflammation.
Many
optical
probes
have
been
developed
contrast
agents
techniques
different
diseases.
In
this
review,
we
summarize
recent
advances
probe
methods
inflammation
organs,
brain,
liver,
lung,
kidney,
intestine,
Finally,
discuss
opportunities
challenges
used
clinic
monitoring
prospect
their
future
detection.
Chemical Reviews,
Journal Year:
2024,
Volume and Issue:
124(16), P. 9225 - 9375
Published: Aug. 13, 2024
Reactive
oxygen
and
nitrogen
species
are
small
reactive
molecules
derived
from
elements
in
the
air─oxygen
nitrogen.
They
produced
biological
systems
to
mediate
fundamental
aspects
of
cellular
signaling
but
must
be
very
tightly
balanced
prevent
indiscriminate
damage
molecules.
Small
molecule
probes
can
transmute
specific
nature
each
into
an
observable
luminescent
signal
(or
even
acoustic
wave)
offer
sensitive
selective
imaging
living
cells
whole
animals.
This
review
focuses
specifically
on
for
superoxide,
hydrogen
peroxide,
hypochlorite,
nitric
oxide,
peroxynitrite
that
provide
a
or
photoacoustic
signal.
Important
background
information
general
photophysical
phenomena,
common
probe
designs,
mechanisms,
modalities
will
provided,
then,
analyte
thoroughly
evaluated.
A
discussion
successes
field
presented,
followed
by
recommendations
improvement
future
outlook
emerging
trends.
Our
objectives
informative,
useful,
thorough
guide
as
well
important
context
compare
ecosystem
chemistries
molecular
scaffolds
has
manifested
within
field.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(6), P. 2481 - 2490
Published: Jan. 31, 2024
Activatable
near-infrared
(NIR)
fluorescent
probes
possess
advantages
of
high
selectivity,
sensitivity,
and
deep
imaging
depth,
holding
great
potential
in
the
early
diagnosis
prognosis
assessment
tumors.
However,
small-molecule
are
largely
limited
due
to
rapid
diffusion
metabolic
clearance
activated
fluorophores
vivo.
Herein,
we
propose
an
efficient
reproducible
novel
strategy
construct
activatable
nanoprobes
through
bioorthogonal
reactions
strong
gold–sulfur
(Au–S)
interactions
achieve
enhanced
permeability
retention
(EPR)
effect,
thereby
achieving
prolonged
high-contrast
tumor
To
demonstrate
merits
this
strategy,
prepared
nanoprobe,
hCy-ALP@AuNP,
for
alkaline
phosphatase
(ALP)
activity
vivo,
whose
nanoscale
properties
facilitate
accumulation
long-term
lesions.
Tumor-overexpressed
ALP
significantly
increased
fluorescence
signal
hCy-ALP@AuNP
NIR
region.
More
importantly,
compared
with
probe
hCy-ALP-N3,
nanoprobe
improved
distribution
time
tumor,
thus
improving
window
accuracy.
Therefore,
platform
has
construction
biomarker-responsive
realize
precise
ACS Materials Letters,
Journal Year:
2024,
Volume and Issue:
6(3), P. 1059 - 1068
Published: Feb. 22, 2024
Drug
toxicity
and
related
drug-induced
liver
injury
(DILI)
have
become
major
biosafety
issues.
Enzyme-activated
fluorescent
probes
been
demonstrated
as
a
powerful
tool
for
the
diagnosis
of
DILI;
however,
they
suffer
from
diffusive
signal
dilution
interference
with
other
organs,
thus
leading
to
misassessment
drug
inaccurate
diagnosis.
Alkaline
phosphatase
(ALP)
is
an
important
biomarker,
alterations
in
enzyme
level
are
tightly
correlated
severity
damage.
Herein,
enzyme-activated
intramolecular
hydrogen
bond
(IMHB)
enhanced
probe
(TPEG-P)
was
developed
ALP
detection
cells
mice
models
DILI.
Differing
previously
reported
intermolecular
charge
transfer
(ICT)
or
excited-state
proton
(ESIPT)
mechanisms,
TPEG-P
enables
precise
recognition
imaging
only
through
activation
bonds
could
situ
sensitively
detect
varying
degrees
caused
by
toxicity.
This
IMHB
strategy
will
advance
development
bioimaging
future.
