Identification of Plant Compounds from Mass Spectrometry Imaging (MSI) DOI Open Access
Nancy Shyrley García‐Rojas, Carlos Daniel Sierra-Álvarez, Hilda Ramos-Aboites

et al.

Published: June 25, 2024

The presence and localization of plant metabolites are indicative physiological processes, e.g., under biotic abiotic stress conditions. Further, the chemical composition parts is related to their quality as food or for medicinal applications. Mass spectrometry imaging (MSI) has become a popular analytical technique exploring visualizing spatial distribution molecules within tissue. This review provides summary mass methods used mapping identifying in tissues. We present benefits disadvantages both vacuum ambient ionization methods, considering direct indirect approaches. Finally, we discuss current limitations annotating perspectives future investigations.

Language: Английский

Metabolite-driven mechanisms reveal chemical ecology of Lehmann Lovegrass (Eragrostis lehmanniana) invasion in North American semi-arid ecosystems DOI Creative Commons
Ben Yang,

M. K. Crawford,

Taylor A. Portman

et al.

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: March 4, 2025

Invasive plants threaten global ecosystems, yet traditional analyses of functional traits cannot fully explain their dominance over co-occurring natives. Metabolomics offers insights into plant invasions, but single-technique studies often miss critical biochemical mechanisms. We employ a multimodal metabolomics approach (¹H NMR, LC MS/MS, FT-ICR-MS, and MALDI-MSI) to investigate the basis Lehmann lovegrass (Eragrostis lehmanniana) invasion in semi-arid North America, comparing it with native grass, Arizona cottontop (Digitaria californica). Our analysis reveals three metabolomic compared cottontop: Enhanced nitrogen allocation shoots, reduced defensive metabolites root layers; increased exudate modulation under stress conditions. These suggest succeeds through adaptation increasing aridity rather than direct competition, demonstrating nutrient-poor environments high phenotypic plasticity response aridity. This integrated provides new mechanistic ecology environmental change.

Language: Английский

Citations

1
Mapping microhabitats of lignocellulose decomposition by a microbial consortium DOI Creative Commons
Marija Veličković, Ruonan Wu, Yuqian Gao

et al.

Nature Chemical Biology, Journal Year: 2024, Volume and Issue: 20(8), P. 1033 - 1043

Published: Feb. 1, 2024

Abstract The leaf-cutter ant fungal garden ecosystem is a naturally evolved model system for efficient plant biomass degradation. Degradation processes mediated by the symbiotic fungus Leucoagaricus gongylophorus are difficult to characterize due dynamic metabolisms and spatial complexity of system. Herein, we performed microscale imaging across 12-µm-thick adjacent sections Atta cephalotes gardens applied metabolome-informed proteome approach map lignin This combines two multiomics mass spectrometry modalities that enabled us visualize colocalized metabolites proteins through garden. Spatially profiled revealed an accumulation lignin-related products, outlining morphologically unique microhabitats. Metaproteomic analyses these microhabitats carbohydrate-degrading enzymes, indicating prominent role in lignocellulose decomposition. Integration data provides comprehensive view underlying biological pathways inform our understanding metabolic matter degradation within micrometer-scale environment.

Language: Английский

Citations

7
Recent strategies for improving MALDI mass spectrometry imaging performance towards low molecular weight compounds DOI
Zhi Sun, Fangfang Wang, Yuwei Liu

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 175, P. 117727 - 117727

Published: April 29, 2024

Language: Английский

Citations

5
Morphological and molecular preservation through universal preparation of fresh-frozen tissue samples for multimodal imaging workflows DOI
Andreas Dannhorn,

Emine Kazanç,

Lucy Flint

et al.

Nature Protocols, Journal Year: 2024, Volume and Issue: 19(9), P. 2685 - 2711

Published: May 28, 2024

Language: Английский

Citations

5
Spatiotemporal metabolic responses to water deficit stress in distinct leaf cell-types of poplar DOI Creative Commons

Vimal Kumar Balasubramanian,

Dušan Veličković, María del Mar Rubio Wilhelmi

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: March 1, 2024

The impact of water-deficit (WD) stress on plant metabolism has been predominantly studied at the whole tissue level. However, tissues are made several distinct cell types with unique and differentiated functions, which limits ‘omics’-based studies to determine only an averaged molecular signature arising from multiple types. Advancements in spatial omics technologies provide opportunity understand mechanisms underlying responses WD cell-type levels. Here, we spatiotemporal metabolic two poplar ( Populus tremula× P. alba ) leaf -palisade vascular cells- using matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI). We identified stress-mediated shifts each type when exposed early prolonged stresses recovery stress. During water-limited conditions, flavonoids phenolic metabolites were exclusively accumulated palisade cells. cells mainly sugars fatty acids during respectively, highlighting functional divergence response By comparing our MALDI-MSI data gas chromatography-mass (GC-MS)-based profile, a few including monosaccharides, hexose phosphates, palmitic acid that showed similar accumulation trend both Overall, this work highlights potential MSI approach complement tissue-based metabolomics techniques provides novel understanding This will help engineer specific pathways cellular level strategic perennial trees like poplars withstand future aberrations environmental conditions increase bioenergy sustainability.

Language: Английский

Citations

4
Seeing is Believing: Developing Multimodal Metabolic Insights at the Molecular Level DOI Creative Commons
Rahuljeet Chadha, Jason A. Guerrero, Lu Wei

et al.

ACS Central Science, Journal Year: 2024, Volume and Issue: unknown

Published: March 21, 2024

This outlook explores how two different molecular imaging approaches might be combined to gain insight into dynamic, subcellular metabolic processes. Specifically, we discuss matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MSI) and stimulated Raman scattering (SRS) microscopy, which have significantly pushed the boundaries of metabolomic analyses in their own right, could create comprehensive images. We first briefly summarize recent advances for each technique. then explore one overcome inherent limitations individual method, by envisioning orthogonal interchangeable workflows. Additionally, delve potential benefits adopting a complementary approach that combines both MSI SRS spectro-microscopy informing on specific chemical structures through functional-group-specific targets. Ultimately, integrating strengths modalities, researchers can achieve more understanding biological systems, enabling precise investigations. synergistic holds substantial promise expand our toolkit studying metabolites complex environments.

Language: Английский

Citations

4
Advanced multi-modal mass spectrometry imaging reveals functional differences of placental villous compartments at microscale resolution DOI Creative Commons
Marija Veličković, Leena Kadam, Joonhoon Kim

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 28, 2025

Language: Английский

Citations

0
Bromine-Based Derivatization of Carboxyl-Containing Metabolites for Liquid Chromatography–Trapped Ion Mobility Spectrometry–Mass Spectrometry DOI
Kaylie I. Kirkwood‐Donelson, Prashant Rai, L. Perera

et al.

Journal of the American Society for Mass Spectrometry, Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

The analysis of small carboxyl-containing metabolites (CCMs), such as tricarboxylic acid (TCA) cycle intermediates, provides highly useful information about the metabolic state cells. However, their detection using liquid chromatography–electrospray ionization–tandem mass spectrometry (LC-ESI-MS/MS) methods can face sensitivity and specificity challenges given low ionization efficiency presence isomers. Ion mobility (IMS), trapped ion (TIMS), additional specificity, but further signal loss occur during separation process. We, therefore, developed a solution to boost CCM chromatographic well leverage IMS. Inspired by carbodiimide-mediated coupling carboxylic acids with 4-bromo-N-methylbenzylamine (4-BNMA) for quantitative analysis, we newly report benefits this reagent TIMS-based measurement. We observed pronounced (orders magnitude) increase in enhanced isomer separations, particularly LC. found that utilization brominated reagent, 4-BNMA, offered unique untargeted Derivatized CCMs displayed shifted out metabolite lipid region TIMS-MS space characteristic isotope patterns, which were leveraged data mining Mass Spectrometry Query Language (MassQL) indication number carboxyl groups. utility our LC-ESI-TIMS-MS/MS method 4-BMA derivatization was demonstrated via characterization alterations expression bone marrow-derived macrophages upon activation lipopolysaccharide. While reprogramming activated has been characterized previously, especially respect TCA novel finding isomeric itaconic, mesaconic, citraconic after 24 h, indicating possible roles inflammatory response.

Language: Английский

Citations

0
Imaging and spatially resolved mass spectrometry applications in nephrology DOI
Brittney Gorman,

Catelynn C. Shafer,

Nagarjunachary Ragi

et al.

Nature Reviews Nephrology, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Language: Английский

Citations

0
Enhancement of in situ detection and imaging of phytohormones in plant tissues by MALDI‐MSI using 2,4‐dihydroxy‐5‐nitrobenzoic acid as a novel matrix DOI
Lulu Chen, Yue Zhang, Qichen Hao

et al.

New Phytologist, Journal Year: 2024, Volume and Issue: 243(5), P. 2021 - 2036

Published: July 16, 2024

Summary Phytohormones possess unique chemical structures, and their physiological effects are regulated through intricate interactions or crosstalk among multiple phytohormones. MALDI‐MSI enables the simultaneous detection imaging of hormones. However, its application for tracing phytohormones is currently restricted by low abundance hormone in plant suboptimal matrix selection. 2,4‐Dihydroxy‐5‐nitrobenzoic acid (DHNBA) was reported as a new MALDI enhanced tissues. DHNBA demonstrates remarkable sensitivity improvement when compared to commonly used matrix, 2,5‐dihydroxybenzoic (DHB), isoprenoid cytokinins ( trans ‐zeatin t Z), dihy‐drozeatin (DHZ), meta ‐topolin m T), N 6 ‐(Δ 2 ‐isopentenyl) adenine (iP)), jasmonic (JA), abscisic (ABA), 1‐aminocyclo‐propane‐1‐carboxylic (ACC) standards. The distinctive properties (i.e. robust UV absorption, uniform deposition, negligible background interference, high ionization efficiency phytohormones) make it an ideal phytohormones, including Z, DHZ, ABA, indole‐3‐acetic (IAA), ACC, various tissues, example germinating seeds, primary/lateral roots, nodules. Employing significantly enhances our capability concurrently track complex phytohormone biosynthesis pathways while providing precise differentiation specific roles played individual within same category. This will propel forward comprehensive exploration phytohormonal functions science.

Language: Английский

Citations

3