Talanta, Journal Year: 2024, Volume and Issue: 286, P. 127457 - 127457
Published: Dec. 24, 2024
Language: Английский
Aggregate, Journal Year: 2024, Volume and Issue: unknown
Published: June 25, 2024
Abstract Infectious diseases present significant challenges to global health, thereby extensively affecting both human society and the economy. In recent years, functional probes have demonstrated remarkable potential as crucial biomedical media for research treatment of infectious diseases. Their applications in realm include pathogen detection, exploration biological mechanisms, development anti‐infective drugs. This review provides a concise introduction severity, classification, pathogenesis Subsequently, we examined distinctiveness design strategies diagnosing treating diseases, shedding light on their rationale using typical examples. We discuss current status associated with clinical implementation probes. Furthermore, explored prospects these diagnosis aims offer novel insights into diagnostic broaden disease treatment.
Language: Английский
Citations
23
Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(33), P. 12532 - 12540
Published: Aug. 8, 2023
Rapid and sensitive antigen detection using a lateral flow immunoassay (LFIA) is crucial for diagnosing infectious diseases due to its simplicity, speed, user-friendly features. However, it remains critical issue explore specific biorecognition elements powerful signal amplification. In this study, taking SARS-CoV-2 as proof of concept, peptide, WFLNDSELIML, binding the spike (S) was identified by nonamplified biopanning method, which exhibited high affinity target, with dissociation constant 9.29 ± 1.55 nM. Molecular docking analysis reveals that peptide binds N-terminal domain S antigen. Then, capture probe angiotensin-converting enzyme 2 probe, peptide-based (pLFIA) without any antibody developed, polydopamine nanosphere (PDA)@MnO2 nanocomposite excellent oxidase-like activity used colorimetric label, exhibiting dual-mode remarkable amplification natural melanin on-demand nanozyme catalytic enhancement. The PDA@MnO2-based pLFIA capable detecting limit 8.01 pg/mL, 18.7 times lower than conventional tagged gold nanoparticles. Additionally, as-proposed can detect up 150 transduction units/mL pseudoviruses spiked in saliva samples. Given outstanding analytical performance, proposed may offer reliable option rapid diagnosis SARS-CoV-2.
Language: Английский
Citations
37
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(24), P. 10064 - 10073
Published: June 6, 2024
The global spread of monkeypox has become a worldwide public healthcare issue. Therefore, there is an urgent need for accurate and sensitive detection methods to effectively control its spreading. Herein, we screened by phage display two peptides M4 (sequence: DPCGERICSIAL) M6 SCSSFLCSLKVG) with good affinity specificity virus (MPXV) B21R protein. To simulate the state peptide in avoid spatial obstacles peptide, GGGSK was added at C terminus named as M4a. Molecular docking shows that M4a are bound different epitopes hydrogen bonds salt-bridge interactions, respectively. Then, selected capture probe, carbonized polymer dots (CPDs) fluorescent colorimetric double-signal peptide/antigen/signal peptide-displayed sandwich ELISA triggered horseradish peroxidase (HRP) through simple internal filtration effect (IFE) constructed. HRP catalyzes H2O2 oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) generate blue oxidized TMB, which can further quench fluorescence CPDs IFE, enabling detect MPXV modes. proposed immunoassay platform sensitivity reliability detection. limit modes 27.8 9.14 pg/mL B21R, Thus, established double-peptide sandwich-based dual-signal provides guidance development reliable antigen capable mutual confirmation, also great potential exploring various analytical strategies other respiratory surveillance.
Language: Английский
Citations
11
Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137298 - 137298
Published: Jan. 1, 2025
Language: Английский
Citations
1
Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 158093 - 158093
Published: Nov. 1, 2024
Language: Английский
Citations
6
Talanta, Journal Year: 2023, Volume and Issue: 266, P. 125093 - 125093
Published: Aug. 18, 2023
Language: Английский
Citations
10
Talanta, Journal Year: 2025, Volume and Issue: 294, P. 128231 - 128231
Published: April 26, 2025
Language: Английский
Citations
0
Journal of Pharmaceutical Analysis, Journal Year: 2025, Volume and Issue: unknown, P. 101345 - 101345
Published: May 1, 2025
Language: Английский
Citations
0
Analysis & Sensing, Journal Year: 2024, Volume and Issue: 4(4)
Published: April 8, 2024
Abstract Signal amplification is critical for the detection of meaningful trace targets in field biomedicine, food analysis, and environmental protection. A growing number signal techniques biosensors involving metal ions have been reported recent years, due to merits simplicity, low cost, high efficiency. This review summarizes advancement outlines signaling methods (i. e. metal‐responsive probes, metal‐catalyzed reactions, regulating catalysis, atom spectrometry) that play biosensing as well their applications. Besides, potential challenges be addressed are discussed.
Language: Английский
Citations
3
Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 23, 2024
Lateral flow immunoassay (LFIA) is valued for its simplicity and rapidity on-site screening, however, it experienced false negatives in real sample analysis due to low sensitivity. Although many signal amplification techniques can improve the sensitivity, they usually require additional complicated steps. To address these issues, taking
Language: Английский
Citations
3