Plugging synthetic DNA nanoparticles into the central dogma of life DOI Creative Commons
Kayla Neyra,

Shirishkant Vrijray Desai,

Divita Mathur

et al.

Chemical Communications, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Recent advancements have engineered an approach to encode genetic sequences in DNA nanoparticles, making them a promising platform for gene therapy. This article highlights design considerations of these structures optimal protein expression.

Language: Английский

Ingenious Dual-Circle DNA Walker-Mediated Electrochemical Biosensor for Rapid and Ultrasensitive Detection of MicroRNA DOI

Song-Yuan Tan,

Tong-lin Hou,

Xiaolong Zhang

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 267, P. 116719 - 116719

Published: Aug. 28, 2024

Language: Английский

Citations

6
Purification of DNA Nanoparticles Using Photocleavable Biotin Tethers DOI
Heather R. Everson, Kayla Neyra, Dylan Valerio Scarton

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(17), P. 22334 - 22343

Published: April 18, 2024

The number of applications self-assembled deoxyribonucleic acid (DNA) origami nanoparticles (DNA NPs) has increased drastically, following the development a variety single-stranded template DNA (ssDNA) that can serve as scaffold strand. In addition to viral genomes, such M13 bacteriophage and lambda DNAs, enzymatically produced ssDNA from various sources is rapidly gaining traction being applied for NP preparation. However, separating fully formed NPs have custom scaffolds crude assembly mixes often multistep process first its enzymatic amplification then isolating assembled excess precursor strands. Only sample ready downstream characterization application. this work, we highlight single-step purification sequence- or M13-derived scaffold-based using photocleavable biotin tethers. only requires an inexpensive ultraviolet (UV) lamp, with up 90% yield high purity are obtained. We show versatility in two multihelix bundle structures wireframe polyhedral architecture.

Language: Английский

Citations

4
Near-infrared light-responsive DNA nanosensor with hyperbranched self-stacking exponential amplification for sensitive and precise live-cell and in vivo imaging DOI
Weilin Chen, Zhuo Chen, Tiantian Zhang

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: 428, P. 137259 - 137259

Published: Jan. 16, 2025

Language: Английский

Citations

0
DNA Nanotechnology in the Undergraduate Laboratory: Toehold-Less Strand Displacement in Switchback DNA DOI Creative Commons

Bharath Raj Madhanagopal,

Arun Richard Chandrasekaran

JACS Au, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Dynamic DNA nanostructures that reconfigure into different shapes are used in several applications biosensing, drug delivery, and data storage. One of the ways to produce such structural transformations is by a process called strand displacement. This laboratory experiment demonstrates displacement reaction two-stranded nanostructure switchback addition third strand. In this process, difference affinity between component strands convert conventional duplex DNA. Students introduced concept through gel electrophoresis quantitative analysis reconfiguration. The presented here an example nanotechnology-based exercises undergraduate setting tailored for adaptation chemistry, biology, or biochemistry with minimal costs.

Language: Английский

Citations

0
DNA Nanoframework-Confined Well-Organized Catalytic Hairpin Assembly for Highly Sensitive and Specific Detection of MiRNA DOI

Yongbin Hou,

Yong Huang,

Yan-e Du

et al.

Talanta, Journal Year: 2025, Volume and Issue: 290, P. 127788 - 127788

Published: Feb. 21, 2025

Language: Английский

Citations

0
Evaluating cadmium-free quantum dots along with mixed nanoparticle clusters as scaffolds for multienzymatic glycolytic channeling DOI Creative Commons
Joyce C. Breger, Drew Lysne, Kimihiro Susumu

et al.

Journal of Nanoparticle Research, Journal Year: 2025, Volume and Issue: 27(3)

Published: March 1, 2025

Language: Английский

Citations

0
Dual-palindrome-incorporated hand-in-hand self-linking bidirectional DNA amplifier within exogenous near-infrared light stimulation for high-performance imaging in living biosystems DOI
Tiantian Zhang, Xiaoming Sun, Weilin Chen

et al.

Talanta, Journal Year: 2025, Volume and Issue: 292, P. 128003 - 128003

Published: March 26, 2025

Language: Английский

Citations

0
DNA‐Encoded Fluorescence Signals for Imaging Analysis DOI Open Access
Xiaowen Cao,

Wenhao Fu,

X Li

et al.

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

Fluorescence imaging has been a powerful technique for the visualization of interest biomolecules. It is widely used in basic biological research, clinical medicine, and other fields. However, fluorescence signals are often too weak detecting with simple devices or complex environments. Besides, limited to about four six dyes, restricting spectral overlap organic fluorophores. DNA nanotechnology including structured dynamic emerges as promising material encode fluorophores, holding great potential improve properties signals. Substantial progresses have achieved DNA-encoded signals, exhibiting novel characteristics applications. This review summarizes various encoding strategies their performance analysis. In this review, different methods impacts on reported, such brightness, photostability, kinetics, multiplexing. application reviewed. Finally, solutions address current challenges suggested, encouraging future development area.

Language: Английский

Citations

0
Isothermal assembly of DNA nanostructures DOI
Arun Richard Chandrasekaran

Chemical Communications, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

DNA nanostructures can be assembled by incubation at constant temperatures in a process called isothermal assembly. Several additives such as denaturing agents, cationic amino acids and natural products aid the assembly process.

Language: Английский

Citations

0
DNA Nanocage-Assisted Size-Selective Recognition and Quantification toward Low-Mass Soluble β-Amyloid Oligomers DOI
Jia Chen, Qi Liu,

Yongchun Fu

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(28), P. 11397 - 11403

Published: June 28, 2024

Low-mass soluble β-amyloid peptide oligomers (LSAβOs) play a crucial role in the pathogenesis of Alzheimer's disease. However, these exhibit heterogeneity terms structure, stability, and stoichiometry, their abundance biofluids is low, making accurate identification challenging. In this study, we developed DNA nanocage-assisted method for selective sizing sensitive quantification LSAβOs serum. Using LSAβO less than 10 kDa (LSAβO10kD) 30 (LSAβO30kD) as models, size-matching rules between nanocages were investigated, two appropriate selected detection LSAβOs, respectively. Both functionalized by encapsulating oligomer's aptamer complementary sequence within cavities. Once entered corresponding nanocage cavity, was released, triggering hybridization chain reaction on an electrochemical sensing platform. The system achieved size-selective discrimination LSAβO10kD with linear range 10–150 pM LSAβO30kD 15–150 pM. Real sample testing confirmed applicability blood-based diagnosis. analysis platform provides accurate, highly selective, approach oligomer analysis, which significant amyloid protein research related disease

Language: Английский

Citations

3