Fluorescent DNA-Silver nanoclusters in food safety detection: From synthesis to application

Talanta, Journal Year: 2024, Volume and Issue: 273, P. 125834 - 125834

Published: March 8, 2024

Language: Английский

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Programmable DNA Templates for Silver Nanoclusters Synthesis To Develop On-Demand FRET Aptasensor

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(25), P. 10391 - 10398

Published: June 6, 2024

DNA-templated silver nanoclusters (AgNCs-DNA) can be synthesized via a one-pot method bypassing the tedious process of biomolecular labeling. Appending an aptamer to DNA templates results in dual-functionalized strands that utilized for synthesizing aptamer-modified AgNCs, thereby enabling development label-free fluorescence aptasensors. However, major challenge lies necessity redesign strand each specific target, thus increasing complexity and hindering widespread application these To overcome this challenge, we designed six (DNA1-DNA6) incorporate AgNCs synthesis A4-linker further coupling. Among all AgNCs-DNA samples, it was found both AgNCs-DNA1 AgNCs-DNA2 stood out their excellent long-term stability. After capturing T4-linker connected with aptamer1 aflatoxin B1 (AFB1), however, only AgNCs-DNA1/aptamer1 maintained This finding highlighted potential as versatile probe on-demand emphasize its benefits aptasensing applications, MoS

Language: Английский

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Aptamer-based electrochemical analysis platform for tumor cells and biomarkers detection

Journal of Electroanalytical Chemistry, Journal Year: 2024, Volume and Issue: 960, P. 118194 - 118194

Published: March 15, 2024

Language: Английский

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Hyperbranched RCA-mediated construction of a dendritic DNA nanostructure for ratiometric fluorescence biosensing

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 412, P. 135797 - 135797

Published: April 10, 2024

Language: Английский

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Dual-mode ratiometric fluorescent and colorimetric sensor for rapid visual detection of Hg2+ using poly(T)-tailed ssDNA-silver nanoclusters

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: 330, P. 125751 - 125751

Published: Jan. 16, 2025

Language: Английский

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DNA Hairpin Dimer-Mediated Dual-Catalysis Circuit for Efficient and Amplifiable Electrochemical Biosensing

Published: Jan. 1, 2025

Language: Английский

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DNA hairpin dimer-mediated dual-catalysis circuit for efficient and amplifiable electrochemical biosensing

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 278, P. 117332 - 117332

Published: March 1, 2025

Language: Английский

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Target-Responsive Conformation Switch of Bihairpin Molecular Beacon to Illustrate Ratiometric Fluorescence Biosensing of Dual-Emissive Silver Nanoclusters

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

It might be intriguing to explore the tailored ability of a bihairpin-structured molecular beacon (bhMB) for improving reaction kinetics and assay sensitivity label-free ratiometric fluorescence specific trigger (T*), in which red green silver nanoclusters (rSNC gSNC) were utilized as two reverse emitting reporters. Unlike traditional dually labeled MB, herein proposed bhMB was designed with hairpin subunits recognition signaling readout. Four functional modules included: complement T* identifiable hairpin, template sequence rSNC gSNC variable consensus loops capable complementarily hybridizing form one paired stem, stems able merge into new loop. Upon introducing T*, conformation switch triggered via affinity linking cognate sequence, thereby reconstructing perfect stem–loop monohairpin structure. As such, mutual transition between nonbinding binding states powered by population-shift mechanism shift thermodynamic equilibrium toward state. Benefiting from structure confinement bhMB, local concentration reactive species increased speed up kinetics. In response route, clustering merged fluorescence, while emission decreased, pointing obtained sensitive signal built-in correction. Thus, this strategy would first example integrate recognizing double-hairpin biosensing potential applications.

Language: Английский

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Robust and ultrasensitive electrochemical detection of ochratoxin a using a highly reactive DNAzyme wired via primer exchange reaction

New Journal of Chemistry, Journal Year: 2024, Volume and Issue: 48(13), P. 5665 - 5673

Published: Jan. 1, 2024

We developed a novel and effective electrochemical biosensor for the sensitive detection of OTA using highly reactive DNAzyme wired via primer exchange reaction.

Language: Английский

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An Oxidative Cleavage-Based Cruciform DNA Nanostructure for In Vivo Hypochlorous Acid Visualization to Monitor Intestinal Inflammation

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(23), P. 9621 - 9628

Published: May 31, 2024

Ulcerative colitis is a persistent inflammatory bowel disease characterized by inflammation and ulceration in the colon gastrointestinal tract. It was indicated that generation of hypochlorous acid (HClO) through enzymatic activity myeloperoxidase significantly linked to ulcerative colitis. In this study, assembling two hairpins (Hpa Hpb) onto quadrivalent cruciform DNA nanostructure, novel HClO-activatable fluorescent probe developed based on nanomaterials (denoted MHDNA), which sensitive, economic, simple, stable. presence HClO, Trigger (T) liberated from MHDNA hydrolysis reaction between HClO phosphorothioate (PS), modified has proved exhibit particular susceptibility HClO. The T subsequently initiated opening Hpa Hpb facilitate catalyzed hairpin assembly (CHA) reaction, resulting changes fluorescence releasing for recycled signal amplification achieve sensitive detection (with limit 9.83 nM). Additionally, MHDNA-based spatial-confinement effect shortens physical distance yields high local concentration reactive hairpins, achieving more rapid kinetics comparison conventional CHA methods. Inspirationally, effectively utilized imaging mice, yielding valuable diagnostic insights

Language: Английский

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