Designing Fluorescent Interfaces at Hotspots in a Plasmonic Nanopore for Homologous Optoelectronic Sensing DOI Open Access

Li‐Dong Chen,

Di Zhang,

Zi‐Hui Li

et al.

Small, Journal Year: 2025, Volume and Issue: 21(9)

Published: Jan. 20, 2025

In this work, a site-selective functionalization strategy is proposed for modifying fluorescent dyes in the plasmonic nanopore, which highlights building optoelectronic dual-signal sensing interfaces at "hotspots" locations to construct multiparameter detection nanosensor. Finite-difference time-domain (FDTD) simulations confirmed high-intensity electromagnetic field due nanostructure. It demonstrated that adjusting distance between nanopore inner wall and fluorophore prevented fluorescence quenching, resulting more than thirty fold enhancement. Upon binding with target analyte, sensor produces homologous yet independent responses cross-validate one another, providing highly accurate analysis even presence of multiple interferences. The platform demonstrates precise, adaptable linear extracellular pH changes single-cell level, making it versatile tool range biosensing applications. By enabling metal nanopores, interface design efficiently exploits enhancement fields achieve high-precision measurements greatly improves sensitivity

Language: Английский

Nano/Micromotor‐Driven SERS for Highly Sensitive and Spatially Controlled Sensing DOI Creative Commons
I. Brian Becerril‐Castro, Verónica Salgueiriño, Miguel A. Correa‐Duarte

et al.

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 34(17)

Published: Dec. 24, 2023

Abstract Nano/micromotors (NMs) are tiny structures capable of converting various forms energy into mechanical motion at the micro and nanoscale. These motors operate in environments characterized by low inertia Reynolds numbers. The potential applications NMs vast, particularly fields biomedicine environmental science. One most intriguing developments this field is integration with surface‐enhanced Raman scattering (SERS) spectroscopy. SERS a powerful analytical technique that enhances intensity molecules, allowing for highly sensitive detection analysis trace amounts substances. This offers precise localized ultrasensing capabilities. combination can also facilitate real‐time imaging inside living organisms. has immense chemical cell biology medical diagnostics prognosis. Herein review describes types their fabrication, incorporation plasmonic nanostructures, creating strong electromagnetic when illuminated light, which turn signals significantly, applications, future prospects areas such as precision medicine, monitoring, possibly even new realms like microscale robotics targeted therapeutics.

Language: Английский

Citations

15
Biomarkers detection by surface-enhanced Raman spectroscopy: Analytical strategies DOI

Jiewen Liu,

Huasheng Lai, Gongke Li

et al.

Microchemical Journal, Journal Year: 2024, Volume and Issue: 203, P. 110912 - 110912

Published: June 9, 2024

Language: Английский

Citations

5
A green, efficient and precise hydrogen therapy of cancer based on in vivo electrochemistry DOI Creative Commons
Guohua Qi, Bo Wang,

Xiangfu Song

et al.

National Science Review, Journal Year: 2019, Volume and Issue: 7(3), P. 660 - 670

Published: Dec. 4, 2019

By combined use of traditional Chinese acupuncture Fe needle electrode and in vivo electrochemistry, we achieved H2 generation tumors a controllable manner exploited it for effective green therapy the first time. The cathodic electrodes working under an applied voltage ∼3 V (with minimal damage to living body) undergo electrochemical reactions acidic tumor area that produce sufficient locally cause cancer cells burst die. Due puncture positioning, microenvironment gas diffusion effect, developed electrochemotherapy (H2-ECT) strategy enables precise large-scale therapy, as demonstrated by treatment diseased mice (glioma breast cancers). Such H2-ECT is simple, highly efficient minimally invasive, requiring no expensive medical equipment or nano materials medication, therefore very promising potential clinical applications.

Language: Английский

Citations

42
Tumor Microenvironment-Activated Degradable Multifunctional Nanoreactor for Synergistic Cancer Therapy and Glucose SERS Feedback DOI Creative Commons
Dan Sun, Guohua Qi,

Kongshuo Ma

et al.

iScience, Journal Year: 2020, Volume and Issue: 23(7), P. 101274 - 101274

Published: June 16, 2020

Integration of disease diagnosis and therapy in vivo by nanotechnology is a challenge the design multifunctional nanocarriers. Herein, we report an intelligent degradable nanoreactor, assembly 4-mercaptobenzonitrile-decorated silver nanoparticles ([email protected]) glucose oxidase (GOx)-loaded metal-organic-framework protected]), which can be activated tumor microenvironment to start catalytic cascade-enhanced chemo-starvation synergistic simultaneous self-sense cellular level. Under mild acidic tumor, nanoreactor will collapse release GOx that triggers cascade reaction vivo, depleting glucose, etching [email protected], producing toxic H2O2, Ag+, Zn2+ ions, all work together inhibit growth. The protected] as SERS nanoprobe reads out concentration noninvasively achieve instant feedback therapeutic progression. This proposes promising example using enzyme-encapsulated biomineralized MOFs effective anticarcinogen for clinical applications.

Language: Английский

Citations

39
Fast Activation and Tracing of Caspase-3 Involved Cell Apoptosis by Combined Electrostimulation and Smart Signal-Amplified SERS Nanoprobes DOI
Guohua Qi, Dan Sun, Yu Tian

et al.

Analytical Chemistry, Journal Year: 2020, Volume and Issue: 92(11), P. 7861 - 7868

Published: May 12, 2020

Caspase-3 is considered as one of the key proteases that can spontaneously regulate life activities cells, and its activation (usually a slow process) will execute apoptosis process cells. Rapid caspase-3 on demand in living-cells therefore highly desired toward precise cancer therapy but it still challenge. Herein, we applied electrostimulus (ES) to achieve fast trigger cell apoptosis, developed smart magnetic-plasmonic assembly nanoprobes (A-nanoprobes) real-time trace cellular at single level. The designer core-satellite A-nanoprobe, working specific activated via disassembly tactic, provides strong "hot spots" improve sensitivity enables SERS sensing upon by ES. Single-cell analysis revealed ES rapidly activate pathway make DNA fragmentation ultimately induce apoptosis. Such method nanoplatform were further used monitor ES-triggered different types, revealing more be for cancerous cells than normal during This strategy platform are promising detecting other enzymes diagnosis treatments.

Language: Английский

Citations

33
Label-Free Single-Particle Surface-Enhanced Raman Spectroscopy Detection of Phosphatidylserine Externalization on Cell Membranes with Multifunctional Micron-Nano Composite Probes DOI
Guohua Qi,

Jiafeng Wang,

Kongshuo Ma

et al.

Analytical Chemistry, Journal Year: 2021, Volume and Issue: 93(4), P. 2183 - 2190

Published: Jan. 7, 2021

Monitoring externalization of phosphatidylserine (PS) and gaining insights into molecular events cell membrane damage are significant for programmed death studies. Herein, by encapsulating zeolitic imidazole frameworks-8 with plasmonic gold nanoparticles to form micron-nano composites using them as a single-particle surface-enhanced Raman spectroscopy (SERS) substrate, we succeeded in real-time discriminating monitoring the PS on membranes during electrostimulus-induced apoptosis. The composite probe provides rich "hot spots" robust anchoring capacity membranes, achieving capture label-free SERS detection externalized PS. By this method, dynamic process differences between cancerous cells normal were clearly revealed which was more serious than that cells. This method is versatile uncovering related mechanisms. work also broadens application metal–organic framework materials advanced biomedical applications.

Language: Английский

Citations

28
Cadmium sulfide as bifunctional mimics of NADH oxidase and cytochrome c reductase takes effect at physiological pH DOI
Hao Wang, Jinxing Chen, Qingfeng Dong

et al.

Nano Research, Journal Year: 2022, Volume and Issue: 15(6), P. 5256 - 5261

Published: March 15, 2022

Language: Английский

Citations

21
Recent Progress in the Application of Metal Organic Frameworks in Surface-Enhanced Raman Scattering Detection DOI Creative Commons

Haojia Qin,

Shuai Zhao, Huaping Gong

et al.

Biosensors, Journal Year: 2023, Volume and Issue: 13(4), P. 479 - 479

Published: April 16, 2023

Metal–organic framework (MOF) compounds are centered on metal ions or ion clusters, forming lattices with a highly ordered periodic porous network structure by connecting organic ligands. As MOFs have the advantages of high porosity, large specific surface area, controllable pore size, etc., they widely used in gas storage, catalysis, adsorption, separation and other fields. SERS substrate based can not only improve sensitivity analysis but also solve problem easy aggregation nanoparticles. By combining SERS, performance is further improved, tremendous research progress has been made recent years. In this review, three methods preparing MOF-based substrates introduced, latest applications biosensors, environment, gases medical treatments discussed. Finally, current status prospects summarized.

Language: Английский

Citations

13
External power-driven micro/nanorobots: Design, fabrication, and functionalization for tumor diagnosis and therapy DOI

Miao An,

Ying Feng, Yang Liu

et al.

Progress in Materials Science, Journal Year: 2023, Volume and Issue: 140, P. 101204 - 101204

Published: Oct. 9, 2023

Language: Английский

Citations

12
Smart Plasmonic Nanozyme Enhances Combined Chemo-photothermal Cancer Therapy and Reveals Tryptophan Metabolic Apoptotic Pathway DOI
Guohua Qi, Ying Zhang, Jiafeng Wang

et al.

Analytical Chemistry, Journal Year: 2019, Volume and Issue: 91(19), P. 12203 - 12211

Published: Sept. 6, 2019

Regulation of the tumor microenvironment is considered to be an intelligent strategy for cancer therapeutics, but related metabolic pathways cell apoptosis still remain a great challenge. Herein, by applying multifunctional carbon dot-decorated Ag/Au bimetallic nanoshells (CDs-Ag/Au NSs, CAANSs) nanoprobes as smart plasmonic nanozymes combined chemo-photothermal therapy, we achieved high efficiency in therapy and revealed tryptophan (Trp) apoptotic pathway. In addition photothermal conversion efficiency, CAANSs can act nanozyme catalyze intracellular H2O2 cytotoxic reactive oxygen species (ROS) superoxide anion (·O2-), response mild acidic cancerous damage cellular DNA. More importantly, Trp pathway during has that participates oxidative stress process, which decomposed produce further formed into anions kill cells under catalytic nanomedicine process. The current work provides effective platform therapeutics promising cancer-related molecular biology studies.

Language: Английский

Citations

33