Next-generation physiology approaches to study microbiome function at single cell level

Nature Reviews Microbiology, Journal Year: 2020, Volume and Issue: 18(4), P. 241 - 256

Published: Feb. 13, 2020

Language: Английский

---

Imaging Cell‐Matrix Adhesions and Collective Migration of Living Cells by Electrochemiluminescence Microscopy

Angewandte Chemie International Edition, Journal Year: 2019, Volume and Issue: 59(1), P. 449 - 456

Published: Oct. 21, 2019

Abstract Cell‐matrix adhesions play essential roles in a variety of biological processes. Herein, we report label‐free method to map cell‐matrix single living cells on an electrode surface by electrochemiluminescence (ECL). An indium tin oxide modified with silica nanochannel membrane was used as the substrate electrode, at which ECL generation from freely diffusing luminophores provided distinct visual contrast between adhesion sites and noncontacted domains, thus selectively revealing former manner. With this methodology, studied spatial distribution, well dynamic variation, strength subcellular level. advancing cell sheet were finally imaged study movement collective migration. A statistical analysis suggests that far side leading edge also have propensity migrate do not act just passive followers.

Language: Английский

---

Single-cell metabolomics: where are we and where are we going?

Current Opinion in Biotechnology, Journal Year: 2022, Volume and Issue: 75, P. 102693 - 102693

Published: Feb. 10, 2022

Single-cell metabolomics with mass spectrometry enables a large variety of metabolites to be simultaneously detected from individual cells, without any preselection or labelling, map phenotypes on the single cell level. Although field is relatively young, it steadily progressing an increasing number active research groups, techniques for sampling and ionization, tools data analysis, applications answer important biomedical environmental questions. In addition, community shows great creativity in overcoming challenges associated low sample volumes, wide range metabolite species, datasets. Here, we briefly discuss publications since 2019 aim provide unfamiliar reader insight into expert update current status field.

Language: Английский

---

Micro‐Lensed Fiber Laser Desorption Mass Spectrometry Imaging Reveals Subcellular Distribution of Drugs within Single Cells

Angewandte Chemie International Edition, Journal Year: 2020, Volume and Issue: 59(41), P. 17864 - 17871

Published: June 30, 2020

Abstract The visualization of temporal and spatial changes in the intracellular environment has great significance for chemistry bioscience research. Mass spectrometry imaging (MSI) plays an important role because its unique advantages, such as being label‐free high throughput, yet it is a challenge laser‐based techniques due to limited lateral resolution. Here, we develop simple, reliable, economic nanoscale MSI approach by introducing desorption laser with micro‐lensed fiber. Using this integrated platform, achieved 300 nm resolution successfully visualized distribution various small‐molecule drugs subcellular locations. Exhaustive dynamic processes anticancer drugs, including releasing from nanoparticle carriers entering nucleus cells, can be readily acquired on organelle scale. Considering simplicity universality device, could easily adapted most mass applications.

Language: Английский

---

Single-Cell Classification Using Mass Spectrometry through Interpretable Machine Learning

Analytical Chemistry, Journal Year: 2020, Volume and Issue: 92(13), P. 9338 - 9347

Published: June 10, 2020

The brain consists of organized ensembles cells that exhibit distinct morphologies, cellular connectivity, and dynamic biochemistries control the executive functions an organism. However, relationships between chemical heterogeneity, cell function, phenotype are not always understood. Recent advancements in matrix-assisted laser desorption/ionization mass spectrometry have enabled high-throughput, multiplexed analysis single cells, capable resolving hundreds molecules each spectrum. We developed a machine learning workflow to classify according their spectra based on groups interest (GOI), e.g., neurons vs astrocytes. Three data sets from various were acquired three different spectrometer platforms representing thousands individual collected used validate classification workflow. trained models achieved >80% accuracy subjected recently instance-based model interpretation framework, SHapley Additive exPlanations (SHAP), which locally assigns feature importance for single-cell SHAP values both local global interpretations our sets, preserving heterogeneity uncovered by while offering ability perform supervised analysis. top contributing features GOI ranked selected using mean absolute values, highlighting specific defined GOI. Our approach provides insight into discriminating profiles through interpretable learning, facilitating downstream validation.

Language: Английский

---

A review on microfluidics manipulation of the extracellular chemical microenvironment and its emerging application to cell analysis

Analytica Chimica Acta, Journal Year: 2020, Volume and Issue: 1125, P. 94 - 113

Published: June 1, 2020

Language: Английский

---

Chemical Analysis of Single Cells and Organelles

Analytical Chemistry, Journal Year: 2020, Volume and Issue: 93(1), P. 41 - 71

Published: Dec. 7, 2020

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTChemical Analysis of Single Cells and OrganellesKeke HuKeke HuDepartment Chemistry Molecular Biology, University Gothenburg, Kemivägen 10, 41296 SwedenMore by Keke HuView Biography, Tho D. K. NguyenTho NguyenDepartment NguyenView Stefania RabascoStefania RabascoDepartment RabascoView Pieter E. OomenPieter OomenDepartment SwedenParaMedir B.V., 1e Energieweg 13, 9301 LK Roden, The NetherlandsMore OomenView Andrew G. Ewing*Andrew EwingDepartment Sweden*Email: [email protected]More EwingView Biographyhttp://orcid.org/0000-0002-2084-0133Cite this: Anal. Chem. 2021, 93, 1, 41–71Publication Date (Web):December 7, 2020Publication History Published online7 December 2020Published inissue 12 January 2021https://doi.org/10.1021/acs.analchem.0c04361Copyright © 2020 American Chemical SocietyRIGHTS & PERMISSIONSACS AuthorChoicewith CC-BYlicenseArticle Views5015Altmetric-Citations10LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated to reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence for given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InReddit (14 MB) Get e-AlertsSUBJECTS:Vesicles,Peptides proteins,Labeling,Microscopy,Electrochemical cells e-Alerts

Language: Английский

---

Electrochemiluminescence Loss in Photobleaching

Angewandte Chemie International Edition, Journal Year: 2021, Volume and Issue: 60(14), P. 7686 - 7690

Published: Jan. 10, 2021

Abstract The effects of photobleaching on electrochemiluminescence (ECL) was investigated for the first time. plasma membrane Chinese Hamster Ovary (CHO) cells labeled with a [Ru(bpy) 3 ] 2+ derivative. Selected regions fixed were photobleached using confocal mode sequential stepwise illumination or cumulatively and they imaged by both ECL photoluminescence (PL). generated model sacrificial coreactant, tri‐ n ‐propylamine. microscopy shows lower emission. We demonstrate linear correlation between decrease PL loss due to labels immobilized CHO membranes. presented strategy provides valuable information fundamentals excited state opens new opportunities exploring cellular membranes combining techniques such as fluorescence recovery after (FRAP) in (FLIP) methods.

Language: Английский

---

Material-assisted mass spectrometric analysis of low molecular weight compounds for biomedical applications

Journal of Materials Chemistry B, Journal Year: 2021, Volume and Issue: 9(17), P. 3622 - 3639

Published: Jan. 1, 2021

This comprehensive review highlights the recent research on a material-assisted mass spectrometric analysis of low molecular weight compounds, including material types, preparation approaches, and biomedical applications.

Language: Английский

---

Quantification of Biocatalytic Transformations by Single Microbial Cells Enabled by Tailored Integration of Droplet Microfluidics and Mass Spectrometry

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(29)

Published: May 5, 2022

Improving the performance of chemical transformations catalysed by microbial biocatalysts requires a deep understanding cellular processes. While heterogeneity characteristics, such as concentration high abundant content, is well studied, little known about reactivity individual cells and its impact on identity, quantity, purity excreted products. Biocatalytic were monitored chemically specific quantifiable at single-cell level integrating droplet microfluidics, cell imaging, mass spectrometry. Product formation rates for Saccharomyces cerevisiae obtained i) incubating nanolitre-sized droplets product accumulation in microfluidic devices, ii) an imaging setup to determine number droplets, iii) electrospray ionisation spectrometry reading contents droplets. These findings now enable study whole-cell biocatalysis resolution.

Language: Английский

---