Aggregation-induced emission luminogens for super-resolution imaging DOI Open Access
Yunfei Zuo, Ryan T. K. Kwok, Jianwei Sun

et al.

Chemical Physics Reviews, Journal Year: 2024, Volume and Issue: 5(1)

Published: Feb. 27, 2024

Over the past three decades, humanity has successfully surpassed Abbe's diffraction limit through development of super-resolution microscopy (SRM), which leads to an increasing demand for specialized fluorescent molecules. The concept aggregation-induced emission (AIE) emerged as a powerful tool in fluorescence imaging since its inception 2001. While thousands distinctive AIE-based molecules have been extensively utilized, their application SRM was not explored until 2013. Although fewer than one hundred works on AIE and published so far, this field is experiencing rapid growth. This review provides comprehensive summary advancements made by these intersecting domains over last decade. recent research outlined, four future directions are highlighted guide design high-quality probes applications that can further advance promote exciting area research.

Language: Английский

Recent advances in super-resolution optical imaging based on aggregation-induced emission DOI

Feng-Yu Zhu,

Li‐Jun Mei,

Rui Tian

et al.

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: 53(7), P. 3350 - 3383

Published: Jan. 1, 2024

Super-resolution imaging has rapidly emerged as an optical microscopy technique, offering advantages of high resolution over the past two decades; achieving improved requires significant efforts in developing super-resolution agents characterized by brightness, contrast and sensitivity to fluorescence switching. Apart from technical requirements systems algorithms, relies on fluorescent dyes with special photophysical or photochemical properties. The concept aggregation-induced emission (AIE) was proposed 2001, coinciding unprecedented advancements innovations technology. AIE probes offer many advantages, including brightness aggregated state, low background signal, a larger Stokes shift, ultra-high photostability, excellent biocompatibility, making them highly promising for applications imaging. In this review, we summarize progress implementation methods provide insights into mechanism AIE-based imaging, switching resulting photochemically-converted emission, electrostatically controlled specific binding-regulated emission. Particularly, principle been achieve spontaneous switching, expanding selection application scenarios probes. By combining molecular design, some comprehensive facilitate AIEgens (AIE-active molecules)

Language: Английский

Citations

47
Versatile organic fluorescent probes for visualizing the dynamics of lipid droplets DOI

Mingyue Cao,

Yunting Liu,

Caihong Xiang

et al.

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 522, P. 216181 - 216181

Published: Sept. 5, 2024

Language: Английский

Citations

19
A Bright Two‐Photon Lipid Droplets Probe with Viscosity‐Enhanced Solvatochromic Emission for Visualizing Lipid Metabolic Disorders in Deep Tissues DOI
Zheng Zheng,

Yuchen Yang,

Pingping Wang

et al.

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(35)

Published: May 8, 2023

Abstract The polarity of lipid droplets (LDs) plays an important role in pathological processes associated with abnormal metabolism. Monitoring the variation LDs cells and tissues is great importance biomedical research clinical diagnosis. However, developing fluorescent LDs‐specific probes high sensitivity, brightness, permeability for deep tissue imaging still challenging. Herein, a push–pull luminogen (DPBT) aggregation‐induced emission, strong solvatochromism, large Stokes shift, solid‐state fluorescence efficiency superior two‐photon absorption facilely developed. lipophilic DPBT can specifically stain biocompatibility good photostability. viscosity‐enhanced solvatochromic emission property enables to visualize brightness contrast, penetration depth under microscopy. lipids various mouse (atherosclerotic plaque, liver, mesenteric adipose tissues) map their distribution reflect metabolic states within those tissues. It found that deposition as well hyperlipoidemia are clearly different from normal mouse. Its excellent properties make promising candidate investigating LDs‐associated physiological live biological samples.

Language: Английский

Citations

33
Blood–Brain Barrier-Penetrative Fluorescent Anticancer Agents Triggering Paraptosis and Ferroptosis for Glioblastoma Therapy DOI
Jiefei Wang,

Mingyue Cao,

Lulu Han

et al.

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(42), P. 28783 - 28794

Published: Oct. 11, 2024

Currently used drugs for glioblastoma (GBM) treatments are ineffective, primarily due to the significant challenges posed by strong drug resistance, poor blood-brain barrier (BBB) permeability, and lack of tumor specificity. Here, we report two cationic fluorescent anticancer agents (TriPEX-ClO

Language: Английский

Citations

12
Fluorescent Solvatochromic Probes for Long‐Term Imaging of Lipid Order in Living Cells DOI Creative Commons

Takuya Tanaka,

Atsushi Matsumoto,

Andery S. Klymchenko

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(17)

Published: March 11, 2024

Abstract High‐resolution spatio‐temporal monitoring of the cell membrane lipid order provides visual insights into complex and sophisticated systems that control cellular physiological functions. Solvatochromic fluorescent probes are highly promising noninvasive visualization tools for identifying ordering microenvironment plasma microdomains. However, conventional probes, although capable structural analysis, lack necessary long‐term photostability required live imaging at level. Here, an ultra‐high‐light‐resistant solvatochromic fluorescence probe, 2‐ N , ‐diethylamino‐7‐(4‐methoxycarbonylphenyl)‐9,9‐dimethylfluorene (FπCM) is reported, which enables division. This probe its derivatives exhibit sufficient wavelengths, brightness, polarity responsiveness, low phototoxicity, remarkable under conditions compared to probes. Therefore, these have potential overcome limitations microscopy, particularly those associated with photobleaching. FπCM can serve as valuable elucidating mechanisms processes bio‐membrane

Language: Английский

Citations

11
Polarity-Sensitive fluorescent probes based on triphenylamine for fluorescence lifetime imaging of lipid droplets DOI
Qian Wang, Dongmei Li, Ze Zhang

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: 330, P. 125694 - 125694

Published: Jan. 2, 2025

Language: Английский

Citations

1
Tuning Molecular Packing by Twisting Structure to Facilely Construct Highly Efficient Solid‐State Fluorophores for Two‐Photon Bioimaging and Photodynamic Therapy DOI
Hui Cao, Ya Gao,

Bingshun Wu

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(27)

Published: March 7, 2024

Abstract The traditional design strategy for constructing highly bright solid‐state luminescent materials relies on incorporating aggregation‐induced emission (AIE) scaffolds, molecular rotors, or bulky substituents to prevent close cofacial packing, which limits the diversity in developing new materials. Herein, a of tuning packing by twisting structure conventional aggregation‐caused quenching fluorophore is proposed endow with AIE effect and enhance fluorescence. Accordingly, series 1,4‐bis(diphenylamino)‐2,5‐disubstituted benzene fluorophores exhibiting characteristics, high fluorescence efficiency (up 0.99), wide color tunability, good near‐infrared (NIR) two‐photon absorption facilely developed. All these luminogens can specifically stain intracellular lipid droplets signal‐to‐noise ratio, biocompatibility, photostability. Besides, exhibit effective reactive oxygen species (ROS) generation capability upon low‐power white light irradiation. Among them, luminogen, named BDBDC, integrating NIR emission, strongest ROS demonstrates superior performances imaging various tissues photodynamic cancer therapy. This philosophy provides way designing practical applications.

Language: Английский

Citations

6
Labeling selectivity of lipid droplets fluorescent probes: Twisted intramolecular charge transfer (TICT) vs intramolecular charge transfer (ICT) DOI

Ri Zhou,

Guannan Liu,

Shengjie Fu

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 264, P. 116624 - 116624

Published: Aug. 7, 2024

Language: Английский

Citations

6
Ultrabright organic fluorescent probe for quantifying the dynamics of cytosolic/nuclear lipid droplets DOI
Guannan Liu,

Huanlong Zheng,

Ri Zhou

et al.

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 241, P. 115707 - 115707

Published: Sept. 27, 2023

Language: Английский

Citations

16
Molecular and Aggregate Synergistic Engineering of Aggregation-Induced Emission Luminogens to Manipulate Optical/Electronic Properties for Efficient and Diversified Functions DOI
Zheng Zheng, Tianyu Yang, Dongyu Li

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(9), P. 8782 - 8795

Published: April 19, 2023

The optical/electronic properties of organic luminescent materials can be regulated by molecular structure modification, which not only requires sophisticated and time-consuming synthesis but also is unable to accurately afford the optical in aggregate state. Herein, a facile strategy synergistic engineering proposed manipulate luminogen, ACIK, solid state for efficient diversified functions. ACIK facilely synthesized exhibits three polymorphic states (ACIK-Y, ACIK-R, ACIK-N) with large emission difference 102 nm from yellow near-infrared (NIR). Their structure-property relationships were investigated crystallographic analyses computational studies. ACIK-Y, most twisted structure, an intriguing color-tuned fluorescence between NIR response multiple stimuli. Shuttle-like ACIK-R microcrystals exhibit waveguide property low loss coefficient 19 dB mm-1. dots display bright NIR-I emission, Stokes shift, strong NIR-II two-photon absorption. show specific lipid droplets-targeting capability successfully applied imaging mouse brain vasculature deep penetration high spatial resolution. This study will inspire more insights developing advanced based on single chromophore practical applications.

Language: Английский

Citations

15