Pomegranate-Bionic Encapsulating Horseradish Peroxidase Using Dopamine Flexible Scaffold-Coated Multishell Porous ZIF-8 To Enhance Immunochromatographic Diagnosis DOI
Mingxuan Song,

Juanxia Xing,

Huan Cai

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(11), P. 10748 - 10759

Published: May 17, 2023

Nanoparticle-natural enzyme complexes are receiving increasing attention as the promising signal reporters for colorimetric lateral flow immunoassay (LFIA). Nonetheless, it remains a challenge to develop nanocomplexes with high loading efficiency, catalytic and brightness. Herein, inspired by pomegranate structure, we reported synthesis of nanocomplex ((HRP@ZIF-8)3@PDA@HRP), using dopamine flexible scaffold-coated multishell porous zeolitic imidazolate framework-8 (ZIF-8) hierarchical scaffold encapsulate horseradish peroxidase (HRP), described its potential promote an ultrasensitive LFIA cardiac troponin I (cTnI). (HRP@ZIF-8)3@PDA@HRP exhibited ultrahigh HRP efficiency activity due epitaxial shell-by-shell overgrowth ZIF-8 scaffold, which provided more cavities immobilization diffusion path substrate. Furthermore, polydopamine (PDA) layer on (HRP@ZIF-8)3 surface both enhanced brightness acted immobilize HRP, further amount enzyme. Following integration LFIA, developed platform achieved test strip assay cTnI pre- postcatalytic naked-eye detection sensitivities 0.5 ng mL–1 0.01 mL–1, respectively, were 4/2- 200/100-fold higher than gold nanoparticles (AuNPs)/PDA-based comparable chemiluminescence immunoassay. Further, quantitative testing results 57 clinical serum samples agreed well data. This work provides ideas design natural enzymes-based encourage applications development early diseases diagnosis.

Language: Английский

Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization DOI Creative Commons
Juan M. Bolívar, John M. Woodley, Roberto Fernandéz‐Lafuente

et al.

Chemical Society Reviews, Journal Year: 2022, Volume and Issue: 51(15), P. 6251 - 6290

Published: Jan. 1, 2022

Enzyme immobilization has been developing since the 1960s and although many industrial biocatalytic processes use technology to improve enzyme performance, still today we are far from full exploitation of field. One clear reason is that evaluate based on only a few experiments not always well-designed. In contrast other reviews subject, here highlight pitfalls using incorrectly designed protocols explain why in cases sub-optimal results obtained. We also describe solutions overcome these challenges come conclusion recent developments material science, bioprocess engineering protein science continue open new opportunities for future. this way, immobilization, being mature discipline, remains as subject high interest where intense research necessary take advantage possibilities.

Language: Английский

Citations

355
MOF-enabled confinement and related effects for chemical catalyst presentation and utilization DOI
Jian Liu, Timothy A. Goetjen, Qining Wang

et al.

Chemical Society Reviews, Journal Year: 2022, Volume and Issue: 51(3), P. 1045 - 1097

Published: Jan. 1, 2022

This review illustrates molecular-scale confinement, containment, isolation, and related concepts to present MOF-centric catalysts realize desired chemical transformations.

Language: Английский

Citations

243
Recent advances in enzyme immobilization based on novel porous framework materials and its applications in biosensing DOI
Yanmei Feng, Ying Xu, Shucheng Liu

et al.

Coordination Chemistry Reviews, Journal Year: 2022, Volume and Issue: 459, P. 214414 - 214414

Published: Feb. 5, 2022

Language: Английский

Citations

212
Confining enzymes in porous organic frameworks: from synthetic strategy and characterization to healthcare applications DOI
Siming Huang, Guosheng Chen, Gangfeng Ouyang

et al.

Chemical Society Reviews, Journal Year: 2022, Volume and Issue: 51(15), P. 6824 - 6863

Published: Jan. 1, 2022

This review comprehensively presents the cutting-edge advances of enzyme confinement using different porous organic frameworks.

Language: Английский

Citations

187
Biocatalysis making waves in organic chemistry DOI Creative Commons
Ulf Hanefeld, Frank Hollmann, Caroline E. Paul

et al.

Chemical Society Reviews, Journal Year: 2021, Volume and Issue: 51(2), P. 594 - 627

Published: Dec. 20, 2021

The many waves of biocatalysis have arisen to solve long-standing synthetic challenges. From industrially applied hydrolases enzymes catalysing selective C–C-bond formation, enables new tools access a plethora compounds.

Language: Английский

Citations

176
Bioinspired Framework Catalysts: From Enzyme Immobilization to Biomimetic Catalysis DOI Creative Commons
Kunyu Wang, Jiaqi Zhang, Yu‐Chuan Hsu

et al.

Chemical Reviews, Journal Year: 2023, Volume and Issue: 123(9), P. 5347 - 5420

Published: April 12, 2023

Enzymatic catalysis has fueled considerable interest from chemists due to its high efficiency and selectivity. However, the structural complexity vulnerability hamper application potentials of enzymes. Driven by practical demand for chemical conversion, there is a long-sought quest bioinspired catalysts reproducing even surpassing functions natural As nanoporous materials with surface areas crystallinity, metal-organic frameworks (MOFs) represent an exquisite case how enzymes their active sites are integrated into porous solids, affording heterogeneous superior stability customizable structures. In this review, we comprehensively summarize advances MOFs catalysis, discuss design principle various MOF-based catalysts, such as MOF-enzyme composites embedded sites, explore utility these in different reactions. The advantages enzyme mimetics also highlighted, including confinement, templating effects, functionality, comparison homogeneous supramolecular catalysts. A perspective provided potential solutions addressing current challenges MOF catalysis.

Language: Английский

Citations

175
Dual Metalation in a Two-Dimensional Covalent Organic Framework for Photocatalytic C–N Cross-Coupling Reactions DOI
Ayan Jati, Kaushik Dey, Maryam Nurhuda

et al.

Journal of the American Chemical Society, Journal Year: 2022, Volume and Issue: 144(17), P. 7822 - 7833

Published: April 21, 2022

Covalent organic frameworks (COFs) are promising hosts in heterogeneous catalysis. Herein, we report a dual metalation strategy single two-dimensional-COF TpBpy for performing variety of C–N cross-coupling reactions. [Ir(ppy)2(CH3CN)2]PF6 [ppy = 2-phenylpyridine], containing two labile CH3CN groups, and NiCl2 used as iridium nickel-metal precursors, respectively, postsynthetic decoration the COF. Moving from traditional approach, focus on COF-backbone host visible-light-mediated nickel-catalyzed coupling The controlled recyclability without deactivation both catalytic centers unique with respect to previously reported strategies. We performed various photoluminescence, electrochemical, kinetic, Hammett correlation studies understand salient features catalyst reaction mechanism. Furthermore, theoretical calculations delineated feasibility electron transfer Ir center Ni inside confined pore metal anchoring within COF backbone prevented nickel-black formation. developed protocol enables selective reproducible diverse range amines (aryl, heteroaryl, alkyl), carbamides, sulfonamides electron-rich, neutral, poor (hetero) aryl iodides up 94% isolated yield. can also be gram scale. establish practical implementation this have applied synthetic late-stage diversification derivatives ibuprofen, naproxen, gemfibrozil, helional, amino acids. methodology could synthesize pharmacophore N,5-diphenyloxazol-2-amine Food Drug Administration-approved drugs, including flufenamic acid, flibanserin, tripelennamine.

Language: Английский

Citations

173
Biocompatibility and biodegradability of metal organic frameworks for biomedical applications DOI Creative Commons
Namita Singh, Somayah Qutub, Niveen M. Khashab

et al.

Journal of Materials Chemistry B, Journal Year: 2021, Volume and Issue: 9(30), P. 5925 - 5934

Published: Jan. 1, 2021

The biocompatibility and biodegradability of metal organic frameworks (MOFs) are discussed to highlight their best biomedical applications up-to-date.

Language: Английский

Citations

152
Recent development of metal-organic framework nanocomposites for biomedical applications DOI Creative Commons
Xueying Ge, Raymond S.K. Wong,

Anee Anisa

et al.

Biomaterials, Journal Year: 2021, Volume and Issue: 281, P. 121322 - 121322

Published: Dec. 15, 2021

Language: Английский

Citations

118
Fine-Tuning the Micro-Environment to Optimize the Catalytic Activity of Enzymes Immobilized in Multivariate Metal–Organic Frameworks DOI
Yiming Li, Jian Yuan, Hao Ren

et al.

Journal of the American Chemical Society, Journal Year: 2021, Volume and Issue: 143(37), P. 15378 - 15390

Published: Sept. 3, 2021

The artificial engineering of an enzyme's structural conformation to enhance its activity is highly desired and challenging. Anisotropic reticular chemistry, best illustrated in the case multivariate metal–organic frameworks (MTV-MOFs), provides a platform modify MOF's pore inner-surface with functionality variations on optimize interior environment specifically targeted property. In this study, we altered ratio linkers zeolitic imidazolate (ZIFs), subclass MOFs, MTV approach demonstrate strategy that allows us encapsulated enzyme by continuously tuning framework–enzyme interaction through hydrophilicity change pores' microenvironment. To systematically study interaction, developed component-adjustment-ternary plot (CAT) method optimal BCL revealed nonlinear correlation, first incremental then decremental, between hydrophilic linker' ratios MTV-ZIF-8. These findings indicated there spatial arrangement functional groups along three-dimensional space across ZIF-8 crystal unique sequence could structure closed-lid open-lid conformations. changes were confirmed FTIR spectra fluorescence studies. optimized BCL@ZIF-8 not only thermally chemically more stable than free solution, but also doubles catalytic reactivity kinetic resolution reaction 99% ee products.

Language: Английский

Citations

116