Orange-Emissive Carbon Dots with High Photostability for Mitochondrial Dynamics Tracking in Living Cells DOI

Nini Xin,

Dongwen Gao,

Borui Su

et al.

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(3), P. 1161 - 1172

Published: Feb. 16, 2023

Mitochondria play significant roles in maintaining a stable internal environment for cell metabolism. Hence, real-time monitoring of the dynamics mitochondria is essential further understanding mitochondria-related diseases. Fluorescent probes provide powerful tools visualizing dynamic processes. However, most mitochondria-targeted are derived from organic molecules with poor photostability, making long-term challenging. Herein, we design novel probe based on carbon dots high performance tracking. Considering that targeting ability CDs related to surface functional groups, which generally determined by reaction precursors, successfully constructed O-CDs emission at 565 nm through solvothermal treatment m-diethylaminophenol. The bright quantum yield 12.61%, mitochondria-targeting ability, and good stability. possess (12.61%), specific outstanding optical Owing abundant hydroxyl ammonium cations surface, showed obvious accumulation colocalization coefficient up 0.90 remained steady even after fixation. Besides, compatibility photostability under various interruptions or long-time irradiation. Therefore, preferable tracking mitochondrial behavior live cells. We first observed fission fusion behaviors HeLa cells, then, size, morphology, distribution physiological pathological conditions were clearly recorded. More importantly, different interactions between lipid droplets during apoptosis mitophagy This study provides potential tool exploring other organelles, promoting research

Language: Английский

A multifunctional chemical toolbox to engineer carbon dots for biomedical and energy applications DOI
Luka Đorđević∞, Francesca Arcudi, Michele Cacioppo

et al.

Nature Nanotechnology, Journal Year: 2022, Volume and Issue: 17(2), P. 112 - 130

Published: Feb. 1, 2022

Language: Английский

Citations

661

Luminescent quantum dots: Synthesis, optical properties, bioimaging and toxicity DOI
Jeladhara Sobhanan,

Jose V. Rival,

Abdulaziz Anas

et al.

Advanced Drug Delivery Reviews, Journal Year: 2023, Volume and Issue: 197, P. 114830 - 114830

Published: April 20, 2023

Language: Английский

Citations

103

Carbon Dots Based Photoinduced Reactions: Advances and Perspective DOI Creative Commons
Yue Yu, Qingsen Zeng,

Songyuan Tao

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(12)

Published: Feb. 3, 2023

Seeking clean energy as an alternative to traditional fossil fuels is the inevitable choice realize sustainable development of society. Photocatalytic technique considered a promising conversion approach store abundant solar into other wieldy carriers like chemical energy. Carbon dots, class fascinating carbon nanomaterials, have already become hotspots in numerous photoelectric researching fields and particularly drawn keen interests metal-free photocatalysts owing strong UV-vis optical absorption, tunable energy-level configuration, superior charge transfer ability, excellent physicochemical stability, facile fabrication, low toxicity, high solubility. In this review, classification, microstructures, general synthetic methods, photoelectrical properties dots are systematically summarized. addition, recent advances based photoinduced reactions including photodegradation, photocatalytic hydrogen generation, CO

Language: Английский

Citations

95

Brightness of fluorescent organic nanomaterials DOI Creative Commons

Anila Hoskere Ashoka,

Ilya O. Aparin, Andreas Reisch

et al.

Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 52(14), P. 4525 - 4548

Published: Jan. 1, 2023

Brightness is a fundamental property of fluorescent nanomaterials reflecting their capacity to absorb and emit light. In sensing materials, brightness crucial for high-sensitivity (bio)molecular detection, while in optical bioimaging it ensures high spatial temporal resolution. Fluorescent organic nanoparticles (NPs) are particularly attractive because superior compared dyes. With the ever-growing diversity nanomaterials, important establish universal principles measuring estimating brightness. This tutorial review provides definitions describes major approaches its analysis based on ensemble single-particle techniques. We present current chemical fight Aggregation-Caused Quenching (ACQ) fluorophores, which challenge design bright nanomaterials. The main classes NPs described, including conjugated polymer NPs, aggregation-induced emission neutral ionic Their other properties systematically compared. Some brightest examples bulk solid-state emissive materials also mentioned. Finally, we analyse importance particle biological applications, such as biosensing. will provide guidelines chemists with improved performance help them estimate compare new literature reports. Moreover, biologists select appropriate imaging applications.

Language: Английский

Citations

77

Multiplexed Biosensing and Bioimaging Using Lanthanide-Based Time-Gated Förster Resonance Energy Transfer DOI
Xue Qiu, Jingyue Xu, Marcelina Cardoso Dos Santos

et al.

Accounts of Chemical Research, Journal Year: 2022, Volume and Issue: 55(4), P. 551 - 564

Published: Jan. 27, 2022

The necessity to scrutinize more and biological molecules interactions both in solution on the cellular level has led an increasing demand for sensitive specific multiplexed diagnostic analysis. Photoluminescence (PL) detection is ideally suited biosensing bioimaging because it rapid there almost unlimited choice of fluorophores that provide a large versatility photophysical properties, including PL intensities, spectra, lifetimes.The most frequently used technique detect multiple parameters from single sample spectral (or color) multiplexing with different fluorophores, such as organic dyes, fluorescent proteins, quantum dots, or lanthanide nanoparticles complexes. In conventional approaches, each fluorophore requires distinct channel excitation wavelength. This drawback can be overcome by Förster resonance energy transfer (FRET) donors other acceptors. lanthanides' spectrally narrow emission bands over broad range overlap several acceptors at once, thereby allowing FRET one donor extremely long lifetimes two important features. First, time-gated (TG) allows efficient suppression background fluorescence environment directly excited Second, temporal multiplexing, which are adjusted interaction acceptor, determine biomolecules and/or their conformation via decays. high signal-to-background ratios, reproducible precise ratiometric homogeneous (washing-free) sensing formats, higher-order capabilities lanthanide-based TG-FRET have resulted significant advances analysis biomolecular recognition. Applications fundamental conformations high-throughput point-of-care vitro diagnostics DNA sequencing advanced optical encoding, using liquid solid samples situ, vitro, vivo sensitivity selectivity.In this Account, we discuss recent development application immunoassays, nucleic acid sensing, imaging. addition highlight importance careful design combination biological, organic, inorganic nanomaterials adjustable donor-acceptor distance determines ultimate performance assays conformational sensors physiological environment. We conclude sharing our vision how progress new concepts, material combinations, instrumentation further advance accelerate its translation into routine clinical practice investigation challenging systems.

Language: Английский

Citations

72

Emerging ultrasmall luminescent nanoprobes for in vivo bioimaging DOI Creative Commons
Shihua Li, Jing Wei, Qiaofeng Yao

et al.

Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 52(5), P. 1672 - 1696

Published: Jan. 1, 2023

Ultrasmall luminescent nanoprobes have shown some unique imaging advantages and application scenarios. In this review, we present a comprehensive summary discussion of ultrasmall towards in vivo PL bioimaging.

Language: Английский

Citations

64

Optimizing Upconversion Nanoparticles for FRET Biosensing DOI
Federico Pini, Laura Francés‐Soriano, Vittoria Andrigo

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(5), P. 4971 - 4984

Published: March 3, 2023

Upconversion nanoparticles (UCNPs) are some of the most promising nanomaterials for bioanalytical and biomedical applications. One important challenge to be still solved is how UCNPs can optimally implemented into Förster resonance energy transfer (FRET) biosensing bioimaging highly sensitive, wash-free, multiplexed, accurate, precise quantitative analysis biomolecules biomolecular interactions. The many possible UCNP architectures composed a core multiple shells doped with different lanthanoid ions at ratios, interaction FRET acceptors distances orientations via interaction, long-lasting pathways from initial excitation final process acceptor emission make experimental determination ideal UCNP-FRET configuration optimal analytical performance real challenge. To overcome this issue, we have developed fully model that requires only few configurations determine system within minutes. We verified our experiments using nine Nd-, Yb-, Er-doped core–shell–shell prototypical DNA hybridization assay Cy3.5 as an dye. Using selected input, determined out all theoretically combinatorial configurations. An extreme economy time, effort, material was accompanied by significant sensitivity increase, which demonstrated powerful feat combining sophisticated but rapid modeling accomplish biosensor.

Language: Английский

Citations

58

N-Heterocyclic Carbene-Stabilized Atomically Precise Metal Nanoclusters DOI
Emily L. Albright, Tetyana I. Levchenko, Viveka K. Kulkarni

et al.

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(9), P. 5759 - 5780

Published: Feb. 19, 2024

This perspective highlights advances in the preparation and understanding of metal nanoclusters stabilized by organic ligands with a focus on N-heterocyclic carbenes (NHCs). We demonstrate need for clear relationship between NHC properties their resulting nanocluster structure properties. emphasize importance balancing stability introduction reactive sites catalytic applications better how these clusters interact environments effective use biological applications. The impact atom-scale simulations, development atomic interaction potentials suitable large-scale molecular dynamics deeper mechanisms behind synthetic methods physical (e.g., bright fluorescence displayed many clusters) are emphasized.

Language: Английский

Citations

55

Molecularly Engineered Room-Temperature Phosphorescence for Biomedical Application: From the Visible toward Second Near-Infrared Window DOI
Baisong Chang, Jie Chen,

Jiasheng Bao

et al.

Chemical Reviews, Journal Year: 2023, Volume and Issue: 123(24), P. 13966 - 14037

Published: Nov. 22, 2023

Phosphorescence, characterized by luminescent lifetimes significantly longer than that of biological autofluorescence under ambient environment, is great value for biomedical applications. Academic evidence fluorescence imaging indicates virtually all metrics (sensitivity, resolution, and penetration depths) are improved when progressing into wavelength regions, especially the recently reported second near-infrared (NIR-II, 1000–1700 nm) window. Although emission probes does matter, it not clear whether guideline "the wavelength, better effect" still suitable developing phosphorescent probes. For tissue-specific bioimaging, long-lived probes, even if they emit visible phosphorescence, enable accurate visualization large deep tissues. studies dealing with bioimaging tiny architectures or dynamic physiopathological activities, prerequisite rigorous planning long-wavelength being aware cooperative contribution long wavelengths improving spatiotemporal depth, sensitivity bioimaging. In this Review, emerging molecular engineering methods room-temperature phosphorescence discussed through lens photophysical mechanisms. We highlight roles from to NIR-II windows toward bioapplications. To appreciate such advances, challenges prospects in rapidly growing described.

Language: Английский

Citations

46

Near‐Infrared Luminescent Materials Incorporating Rare Earth/Transition Metal Ions: From Materials to Applications DOI

Hua‐Hui Li,

Ya‐Kun Wang, Liang‐Sheng Liao

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(30)

Published: May 11, 2024

Abstract The spotlight has shifted to near‐infrared (NIR) luminescent materials emitting beyond 1000 nm, with growing interest due their unique characteristics. ability of NIR‐II emission (1000–1700 nm) penetrate deeply and transmit independently positions these NIR for applications in optical‐communication devices, bioimaging, photodetectors. combination rare earth metals/transition metals a variety matrix provides new platform creating chemical physical properties science device applications. In this review, the recent advancements activated by transition metal ions are summarized role spanning sensing, optoelectronics is illustrated. It started various synthesis techniques explored how earths/transition can be skillfully incorporated into matrixes, thereby endowing them discussion strategies enhancing excitation absorption efficiency, spotlighting innovations like dye sensitization surface plasmon resonance effects then extended. Subsequently, significant focus placed on functionalization Finally, comprehensive analysis challenges proposed earth/transition ion‐doped materials, summarizing insights each section provided.

Language: Английский

Citations

45