ACS Sensors,
Journal Year:
2023,
Volume and Issue:
8(3), P. 1161 - 1172
Published: Feb. 16, 2023
Mitochondria
play
significant
roles
in
maintaining
a
stable
internal
environment
for
cell
metabolism.
Hence,
real-time
monitoring
of
the
dynamics
mitochondria
is
essential
further
understanding
mitochondria-related
diseases.
Fluorescent
probes
provide
powerful
tools
visualizing
dynamic
processes.
However,
most
mitochondria-targeted
are
derived
from
organic
molecules
with
poor
photostability,
making
long-term
challenging.
Herein,
we
design
novel
probe
based
on
carbon
dots
high
performance
tracking.
Considering
that
targeting
ability
CDs
related
to
surface
functional
groups,
which
generally
determined
by
reaction
precursors,
successfully
constructed
O-CDs
emission
at
565
nm
through
solvothermal
treatment
m-diethylaminophenol.
The
bright
quantum
yield
12.61%,
mitochondria-targeting
ability,
and
good
stability.
possess
(12.61%),
specific
outstanding
optical
Owing
abundant
hydroxyl
ammonium
cations
surface,
showed
obvious
accumulation
colocalization
coefficient
up
0.90
remained
steady
even
after
fixation.
Besides,
compatibility
photostability
under
various
interruptions
or
long-time
irradiation.
Therefore,
preferable
tracking
mitochondrial
behavior
live
cells.
We
first
observed
fission
fusion
behaviors
HeLa
cells,
then,
size,
morphology,
distribution
physiological
pathological
conditions
were
clearly
recorded.
More
importantly,
different
interactions
between
lipid
droplets
during
apoptosis
mitophagy
This
study
provides
potential
tool
exploring
other
organelles,
promoting
research
Advanced Science,
Journal Year:
2023,
Volume and Issue:
10(12)
Published: Feb. 3, 2023
Seeking
clean
energy
as
an
alternative
to
traditional
fossil
fuels
is
the
inevitable
choice
realize
sustainable
development
of
society.
Photocatalytic
technique
considered
a
promising
conversion
approach
store
abundant
solar
into
other
wieldy
carriers
like
chemical
energy.
Carbon
dots,
class
fascinating
carbon
nanomaterials,
have
already
become
hotspots
in
numerous
photoelectric
researching
fields
and
particularly
drawn
keen
interests
metal-free
photocatalysts
owing
strong
UV-vis
optical
absorption,
tunable
energy-level
configuration,
superior
charge
transfer
ability,
excellent
physicochemical
stability,
facile
fabrication,
low
toxicity,
high
solubility.
In
this
review,
classification,
microstructures,
general
synthetic
methods,
photoelectrical
properties
dots
are
systematically
summarized.
addition,
recent
advances
based
photoinduced
reactions
including
photodegradation,
photocatalytic
hydrogen
generation,
CO
Chemical Society Reviews,
Journal Year:
2023,
Volume and Issue:
52(14), P. 4525 - 4548
Published: Jan. 1, 2023
Brightness
is
a
fundamental
property
of
fluorescent
nanomaterials
reflecting
their
capacity
to
absorb
and
emit
light.
In
sensing
materials,
brightness
crucial
for
high-sensitivity
(bio)molecular
detection,
while
in
optical
bioimaging
it
ensures
high
spatial
temporal
resolution.
Fluorescent
organic
nanoparticles
(NPs)
are
particularly
attractive
because
superior
compared
dyes.
With
the
ever-growing
diversity
nanomaterials,
important
establish
universal
principles
measuring
estimating
brightness.
This
tutorial
review
provides
definitions
describes
major
approaches
its
analysis
based
on
ensemble
single-particle
techniques.
We
present
current
chemical
fight
Aggregation-Caused
Quenching
(ACQ)
fluorophores,
which
challenge
design
bright
nanomaterials.
The
main
classes
NPs
described,
including
conjugated
polymer
NPs,
aggregation-induced
emission
neutral
ionic
Their
other
properties
systematically
compared.
Some
brightest
examples
bulk
solid-state
emissive
materials
also
mentioned.
Finally,
we
analyse
importance
particle
biological
applications,
such
as
biosensing.
will
provide
guidelines
chemists
with
improved
performance
help
them
estimate
compare
new
literature
reports.
Moreover,
biologists
select
appropriate
imaging
applications.
Accounts of Chemical Research,
Journal Year:
2022,
Volume and Issue:
55(4), P. 551 - 564
Published: Jan. 27, 2022
The
necessity
to
scrutinize
more
and
biological
molecules
interactions
both
in
solution
on
the
cellular
level
has
led
an
increasing
demand
for
sensitive
specific
multiplexed
diagnostic
analysis.
Photoluminescence
(PL)
detection
is
ideally
suited
biosensing
bioimaging
because
it
rapid
there
almost
unlimited
choice
of
fluorophores
that
provide
a
large
versatility
photophysical
properties,
including
PL
intensities,
spectra,
lifetimes.The
most
frequently
used
technique
detect
multiple
parameters
from
single
sample
spectral
(or
color)
multiplexing
with
different
fluorophores,
such
as
organic
dyes,
fluorescent
proteins,
quantum
dots,
or
lanthanide
nanoparticles
complexes.
In
conventional
approaches,
each
fluorophore
requires
distinct
channel
excitation
wavelength.
This
drawback
can
be
overcome
by
Förster
resonance
energy
transfer
(FRET)
donors
other
acceptors.
lanthanides'
spectrally
narrow
emission
bands
over
broad
range
overlap
several
acceptors
at
once,
thereby
allowing
FRET
one
donor
extremely
long
lifetimes
two
important
features.
First,
time-gated
(TG)
allows
efficient
suppression
background
fluorescence
environment
directly
excited
Second,
temporal
multiplexing,
which
are
adjusted
interaction
acceptor,
determine
biomolecules
and/or
their
conformation
via
decays.
high
signal-to-background
ratios,
reproducible
precise
ratiometric
homogeneous
(washing-free)
sensing
formats,
higher-order
capabilities
lanthanide-based
TG-FRET
have
resulted
significant
advances
analysis
biomolecular
recognition.
Applications
fundamental
conformations
high-throughput
point-of-care
vitro
diagnostics
DNA
sequencing
advanced
optical
encoding,
using
liquid
solid
samples
situ,
vitro,
vivo
sensitivity
selectivity.In
this
Account,
we
discuss
recent
development
application
immunoassays,
nucleic
acid
sensing,
imaging.
addition
highlight
importance
careful
design
combination
biological,
organic,
inorganic
nanomaterials
adjustable
donor-acceptor
distance
determines
ultimate
performance
assays
conformational
sensors
physiological
environment.
We
conclude
sharing
our
vision
how
progress
new
concepts,
material
combinations,
instrumentation
further
advance
accelerate
its
translation
into
routine
clinical
practice
investigation
challenging
systems.
Chemical Society Reviews,
Journal Year:
2023,
Volume and Issue:
52(5), P. 1672 - 1696
Published: Jan. 1, 2023
Ultrasmall
luminescent
nanoprobes
have
shown
some
unique
imaging
advantages
and
application
scenarios.
In
this
review,
we
present
a
comprehensive
summary
discussion
of
ultrasmall
towards
in
vivo
PL
bioimaging.
ACS Nano,
Journal Year:
2023,
Volume and Issue:
17(5), P. 4971 - 4984
Published: March 3, 2023
Upconversion
nanoparticles
(UCNPs)
are
some
of
the
most
promising
nanomaterials
for
bioanalytical
and
biomedical
applications.
One
important
challenge
to
be
still
solved
is
how
UCNPs
can
optimally
implemented
into
Förster
resonance
energy
transfer
(FRET)
biosensing
bioimaging
highly
sensitive,
wash-free,
multiplexed,
accurate,
precise
quantitative
analysis
biomolecules
biomolecular
interactions.
The
many
possible
UCNP
architectures
composed
a
core
multiple
shells
doped
with
different
lanthanoid
ions
at
ratios,
interaction
FRET
acceptors
distances
orientations
via
interaction,
long-lasting
pathways
from
initial
excitation
final
process
acceptor
emission
make
experimental
determination
ideal
UCNP-FRET
configuration
optimal
analytical
performance
real
challenge.
To
overcome
this
issue,
we
have
developed
fully
model
that
requires
only
few
configurations
determine
system
within
minutes.
We
verified
our
experiments
using
nine
Nd-,
Yb-,
Er-doped
core–shell–shell
prototypical
DNA
hybridization
assay
Cy3.5
as
an
dye.
Using
selected
input,
determined
out
all
theoretically
combinatorial
configurations.
An
extreme
economy
time,
effort,
material
was
accompanied
by
significant
sensitivity
increase,
which
demonstrated
powerful
feat
combining
sophisticated
but
rapid
modeling
accomplish
biosensor.
Journal of the American Chemical Society,
Journal Year:
2024,
Volume and Issue:
146(9), P. 5759 - 5780
Published: Feb. 19, 2024
This
perspective
highlights
advances
in
the
preparation
and
understanding
of
metal
nanoclusters
stabilized
by
organic
ligands
with
a
focus
on
N-heterocyclic
carbenes
(NHCs).
We
demonstrate
need
for
clear
relationship
between
NHC
properties
their
resulting
nanocluster
structure
properties.
emphasize
importance
balancing
stability
introduction
reactive
sites
catalytic
applications
better
how
these
clusters
interact
environments
effective
use
biological
applications.
The
impact
atom-scale
simulations,
development
atomic
interaction
potentials
suitable
large-scale
molecular
dynamics
deeper
mechanisms
behind
synthetic
methods
physical
(e.g.,
bright
fluorescence
displayed
many
clusters)
are
emphasized.
Chemical Reviews,
Journal Year:
2023,
Volume and Issue:
123(24), P. 13966 - 14037
Published: Nov. 22, 2023
Phosphorescence,
characterized
by
luminescent
lifetimes
significantly
longer
than
that
of
biological
autofluorescence
under
ambient
environment,
is
great
value
for
biomedical
applications.
Academic
evidence
fluorescence
imaging
indicates
virtually
all
metrics
(sensitivity,
resolution,
and
penetration
depths)
are
improved
when
progressing
into
wavelength
regions,
especially
the
recently
reported
second
near-infrared
(NIR-II,
1000–1700
nm)
window.
Although
emission
probes
does
matter,
it
not
clear
whether
guideline
"the
wavelength,
better
effect"
still
suitable
developing
phosphorescent
probes.
For
tissue-specific
bioimaging,
long-lived
probes,
even
if
they
emit
visible
phosphorescence,
enable
accurate
visualization
large
deep
tissues.
studies
dealing
with
bioimaging
tiny
architectures
or
dynamic
physiopathological
activities,
prerequisite
rigorous
planning
long-wavelength
being
aware
cooperative
contribution
long
wavelengths
improving
spatiotemporal
depth,
sensitivity
bioimaging.
In
this
Review,
emerging
molecular
engineering
methods
room-temperature
phosphorescence
discussed
through
lens
photophysical
mechanisms.
We
highlight
roles
from
to
NIR-II
windows
toward
bioapplications.
To
appreciate
such
advances,
challenges
prospects
in
rapidly
growing
described.
Advanced Materials,
Journal Year:
2024,
Volume and Issue:
36(30)
Published: May 11, 2024
Abstract
The
spotlight
has
shifted
to
near‐infrared
(NIR)
luminescent
materials
emitting
beyond
1000
nm,
with
growing
interest
due
their
unique
characteristics.
ability
of
NIR‐II
emission
(1000–1700
nm)
penetrate
deeply
and
transmit
independently
positions
these
NIR
for
applications
in
optical‐communication
devices,
bioimaging,
photodetectors.
combination
rare
earth
metals/transition
metals
a
variety
matrix
provides
new
platform
creating
chemical
physical
properties
science
device
applications.
In
this
review,
the
recent
advancements
activated
by
transition
metal
ions
are
summarized
role
spanning
sensing,
optoelectronics
is
illustrated.
It
started
various
synthesis
techniques
explored
how
earths/transition
can
be
skillfully
incorporated
into
matrixes,
thereby
endowing
them
discussion
strategies
enhancing
excitation
absorption
efficiency,
spotlighting
innovations
like
dye
sensitization
surface
plasmon
resonance
effects
then
extended.
Subsequently,
significant
focus
placed
on
functionalization
Finally,
comprehensive
analysis
challenges
proposed
earth/transition
ion‐doped
materials,
summarizing
insights
each
section
provided.