A multifunctional chemical toolbox to engineer carbon dots for biomedical and energy applications

Nature Nanotechnology, Journal Year: 2022, Volume and Issue: 17(2), P. 112 - 130

Published: Feb. 1, 2022

Language: Английский

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Luminescent quantum dots: Synthesis, optical properties, bioimaging and toxicity

Advanced Drug Delivery Reviews, Journal Year: 2023, Volume and Issue: 197, P. 114830 - 114830

Published: April 20, 2023

Language: Английский

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Carbon Dots Based Photoinduced Reactions: Advances and Perspective

Advanced Science, Journal Year: 2023, Volume and Issue: 10(12)

Published: Feb. 3, 2023

Seeking clean energy as an alternative to traditional fossil fuels is the inevitable choice realize sustainable development of society. Photocatalytic technique considered a promising conversion approach store abundant solar into other wieldy carriers like chemical energy. Carbon dots, class fascinating carbon nanomaterials, have already become hotspots in numerous photoelectric researching fields and particularly drawn keen interests metal-free photocatalysts owing strong UV-vis optical absorption, tunable energy-level configuration, superior charge transfer ability, excellent physicochemical stability, facile fabrication, low toxicity, high solubility. In this review, classification, microstructures, general synthetic methods, photoelectrical properties dots are systematically summarized. addition, recent advances based photoinduced reactions including photodegradation, photocatalytic hydrogen generation, CO

Language: Английский

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Brightness of fluorescent organic nanomaterials

Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 52(14), P. 4525 - 4548

Published: Jan. 1, 2023

Brightness is a fundamental property of fluorescent nanomaterials reflecting their capacity to absorb and emit light. In sensing materials, brightness crucial for high-sensitivity (bio)molecular detection, while in optical bioimaging it ensures high spatial temporal resolution. Fluorescent organic nanoparticles (NPs) are particularly attractive because superior compared dyes. With the ever-growing diversity nanomaterials, important establish universal principles measuring estimating brightness. This tutorial review provides definitions describes major approaches its analysis based on ensemble single-particle techniques. We present current chemical fight Aggregation-Caused Quenching (ACQ) fluorophores, which challenge design bright nanomaterials. The main classes NPs described, including conjugated polymer NPs, aggregation-induced emission neutral ionic Their other properties systematically compared. Some brightest examples bulk solid-state emissive materials also mentioned. Finally, we analyse importance particle biological applications, such as biosensing. will provide guidelines chemists with improved performance help them estimate compare new literature reports. Moreover, biologists select appropriate imaging applications.

Language: Английский

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Multiplexed Biosensing and Bioimaging Using Lanthanide-Based Time-Gated Förster Resonance Energy Transfer

Accounts of Chemical Research, Journal Year: 2022, Volume and Issue: 55(4), P. 551 - 564

Published: Jan. 27, 2022

The necessity to scrutinize more and biological molecules interactions both in solution on the cellular level has led an increasing demand for sensitive specific multiplexed diagnostic analysis. Photoluminescence (PL) detection is ideally suited biosensing bioimaging because it rapid there almost unlimited choice of fluorophores that provide a large versatility photophysical properties, including PL intensities, spectra, lifetimes.The most frequently used technique detect multiple parameters from single sample spectral (or color) multiplexing with different fluorophores, such as organic dyes, fluorescent proteins, quantum dots, or lanthanide nanoparticles complexes. In conventional approaches, each fluorophore requires distinct channel excitation wavelength. This drawback can be overcome by Förster resonance energy transfer (FRET) donors other acceptors. lanthanides' spectrally narrow emission bands over broad range overlap several acceptors at once, thereby allowing FRET one donor extremely long lifetimes two important features. First, time-gated (TG) allows efficient suppression background fluorescence environment directly excited Second, temporal multiplexing, which are adjusted interaction acceptor, determine biomolecules and/or their conformation via decays. high signal-to-background ratios, reproducible precise ratiometric homogeneous (washing-free) sensing formats, higher-order capabilities lanthanide-based TG-FRET have resulted significant advances analysis biomolecular recognition. Applications fundamental conformations high-throughput point-of-care vitro diagnostics DNA sequencing advanced optical encoding, using liquid solid samples situ, vitro, vivo sensitivity selectivity.In this Account, we discuss recent development application immunoassays, nucleic acid sensing, imaging. addition highlight importance careful design combination biological, organic, inorganic nanomaterials adjustable donor-acceptor distance determines ultimate performance assays conformational sensors physiological environment. We conclude sharing our vision how progress new concepts, material combinations, instrumentation further advance accelerate its translation into routine clinical practice investigation challenging systems.

Language: Английский

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Emerging ultrasmall luminescent nanoprobes for in vivo bioimaging

Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 52(5), P. 1672 - 1696

Published: Jan. 1, 2023

Ultrasmall luminescent nanoprobes have shown some unique imaging advantages and application scenarios. In this review, we present a comprehensive summary discussion of ultrasmall towards in vivo PL bioimaging.

Language: Английский

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Optimizing Upconversion Nanoparticles for FRET Biosensing

ACS Nano, Journal Year: 2023, Volume and Issue: 17(5), P. 4971 - 4984

Published: March 3, 2023

Upconversion nanoparticles (UCNPs) are some of the most promising nanomaterials for bioanalytical and biomedical applications. One important challenge to be still solved is how UCNPs can optimally implemented into Förster resonance energy transfer (FRET) biosensing bioimaging highly sensitive, wash-free, multiplexed, accurate, precise quantitative analysis biomolecules biomolecular interactions. The many possible UCNP architectures composed a core multiple shells doped with different lanthanoid ions at ratios, interaction FRET acceptors distances orientations via interaction, long-lasting pathways from initial excitation final process acceptor emission make experimental determination ideal UCNP-FRET configuration optimal analytical performance real challenge. To overcome this issue, we have developed fully model that requires only few configurations determine system within minutes. We verified our experiments using nine Nd-, Yb-, Er-doped core–shell–shell prototypical DNA hybridization assay Cy3.5 as an dye. Using selected input, determined out all theoretically combinatorial configurations. An extreme economy time, effort, material was accompanied by significant sensitivity increase, which demonstrated powerful feat combining sophisticated but rapid modeling accomplish biosensor.

Language: Английский

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N-Heterocyclic Carbene-Stabilized Atomically Precise Metal Nanoclusters

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(9), P. 5759 - 5780

Published: Feb. 19, 2024

This perspective highlights advances in the preparation and understanding of metal nanoclusters stabilized by organic ligands with a focus on N-heterocyclic carbenes (NHCs). We demonstrate need for clear relationship between NHC properties their resulting nanocluster structure properties. emphasize importance balancing stability introduction reactive sites catalytic applications better how these clusters interact environments effective use biological applications. The impact atom-scale simulations, development atomic interaction potentials suitable large-scale molecular dynamics deeper mechanisms behind synthetic methods physical (e.g., bright fluorescence displayed many clusters) are emphasized.

Language: Английский

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Molecularly Engineered Room-Temperature Phosphorescence for Biomedical Application: From the Visible toward Second Near-Infrared Window

Chemical Reviews, Journal Year: 2023, Volume and Issue: 123(24), P. 13966 - 14037

Published: Nov. 22, 2023

Phosphorescence, characterized by luminescent lifetimes significantly longer than that of biological autofluorescence under ambient environment, is great value for biomedical applications. Academic evidence fluorescence imaging indicates virtually all metrics (sensitivity, resolution, and penetration depths) are improved when progressing into wavelength regions, especially the recently reported second near-infrared (NIR-II, 1000–1700 nm) window. Although emission probes does matter, it not clear whether guideline "the wavelength, better effect" still suitable developing phosphorescent probes. For tissue-specific bioimaging, long-lived probes, even if they emit visible phosphorescence, enable accurate visualization large deep tissues. studies dealing with bioimaging tiny architectures or dynamic physiopathological activities, prerequisite rigorous planning long-wavelength being aware cooperative contribution long wavelengths improving spatiotemporal depth, sensitivity bioimaging. In this Review, emerging molecular engineering methods room-temperature phosphorescence discussed through lens photophysical mechanisms. We highlight roles from to NIR-II windows toward bioapplications. To appreciate such advances, challenges prospects in rapidly growing described.

Language: Английский

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Molecular imprinting-based indirect fluorescence detection strategy implemented on paper chip for non-fluorescent microcystin

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Oct. 17, 2023

Fluorescence analysis is a fast and sensitive method, has great potential application in trace detection of environmental toxins. However, many important toxins are non-fluorescent substances, it still challenge to construct fluorescence method for substances. Here, by means charge transfer effect smart molecular imprinting technology, we report indirect fluorescent sensing mechanism (IFSM) microcystin (MC-RR) selected as model target. A imprinted thin film immobilized on the surface zinc ferrite nanoparticles (ZnFe2O4 NPs) using arginine, dummy fragment MC-RR. By implementation IFSM paper-based microfluidic chip, versatile platform quantitative assay MC-RR developed at level (the limit 0.43 μg/L time 20 min) real water samples without any pretreatment. Importantly, proposed can be easily modified extended wide variety species which lack direct interaction with substrate. This work offers possibility meet requirements on-site may explore applications molecularly sensors.

Language: Английский

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