Angewandte Chemie International Edition,
Journal Year:
2023,
Volume and Issue:
62(22)
Published: March 3, 2023
Lipopolysaccharides
from
Bacteroides
vulgatus
represent
interesting
targets
for
the
treatment
of
inflammatory
bowel
diseases.
However,
efficient
access
to
long,
branched
and
complex
lipopolysaccharides
remains
challenging.
Herein,
we
report
modular
synthesis
a
tridecasaccharide
vulgates
through
an
orthogonal
one-pot
glycosylation
strategy
based
on
glycosyl
ortho-(1-phenylvinyl)benzoates,
which
avoids
issues
thioglycoside-based
synthesis.
Our
approach
also
features:
1)
5,7-O-di-tert-butylsilylene-directed
stereoselective
construction
α-Kdo
linkage;
2)
hydrogen-bond-mediated
aglycone
delivery
formation
β-mannosidic
bonds;
3)
remote
anchimeric
assistance
assembly
α-fucosyl
4)
several
synthetic
steps
strategic
use
protecting
groups
streamline
oligosaccharide
assembly;
5)
convergent
[1+6+6]
target.
Abstract
The
gut
microbiota
is
a
complex
community
of
microorganisms
inhabiting
the
intestinal
tract,
which
plays
vital
role
in
human
health.
It
intricately
involved
metabolism,
and
it
also
affects
diverse
physiological
processes.
gut–lung
axis
bidirectional
pathway
between
gastrointestinal
tract
lungs.
Recent
research
has
shown
that
microbiome
crucial
immune
response
regulation
lungs
development
lung
diseases.
In
this
review,
we
present
interrelated
factors
concerning
associated
metabolites
pulmonary
hypertension
(PH),
lethal
disease
characterized
by
elevated
vascular
pressure
resistance.
Our
team
explored
gut‐microbiota‐derived
cardiovascular
diseases
established
correlation
such
as
putrescine,
succinate,
trimethylamine
N‐oxide
(TMAO),
N,
N‐trimethyl‐5‐aminovaleric
acid
with
Furthermore,
found
specific
metabolites,
TMAO
betaine,
have
significant
clinical
value
PH,
suggesting
their
potential
biomarkers
management.
detailing
interplay
microbiota,
underscored
therapeutic
approaches
modulating
microbiota.
Ultimately,
endeavor
to
alleviate
substantial
socioeconomic
burden
disease.
This
review
presents
unique
exploratory
analysis
link
intending
propel
further
investigations
axis.
Deleted Journal,
Journal Year:
2024,
Volume and Issue:
1(1), P. 36 - 45
Published: Jan. 17, 2024
As
the
most
complex
post-translational
modification,
glycosylation
involves
covalent
linking
of
glycan(s)
to
a
biomolecule.
The
proteins,
peptides,
lipids,
and
nucleic
acids
results
in
formation
diverse
glycoconjugates,
like
glycoproteins,
peptidoglycans,
glycopeptides,
glycolipids,
lipopolysaccharides,
glycoRNAs,
glycoconjugates
are
also
ubiquitous
on
surface
extracellular
vesicles
(EVs),
cells,
bacteria,
viruses.
conjugated
glycans
integral
cell
recognition
(e.g.,
adhesion
homing),
trafficking,
immune
recognition,
hormone-receptor
binding,
protein
folding
stabilization,
many
other
biological
processes.
Currently,
glycan-conjugated
objects
have
found
widespread
applications
disease
diagnosis
exosomes,
circulating
tumor
cells)
therapy
therapeutic
monoclonal
antibodies).
Over
past
decades,
variety
methods
been
established
for
quantitative
assay
objects,
light
their
significant
diagnostic
values,
using
antibodies,
aptamers,
or
affinity
peptides
as
ligands.
Relative
methods,
aptamer-based
electrochemical
biosensors
(i.e.,
aptasensors)
attracted
much
attention
recent
years
detection
targets,
by
virtue
intrinsic
advantages
such
low
cost,
good
portability,
simple
instrument,
superior
stability
anti-interference
capability,
high
sensitivity
selectivity.
This
review
summarizes
biological,
diagnostic,
significance
then
presents
progress
aptasensing
including
lipopolysaccharides
(LPSs),
cells
(CTCs),
pathogenic
microbes
bacteria
viruses).
Moreover,
challenges
future
prospects
discussed.
Gut Microbes,
Journal Year:
2024,
Volume and Issue:
16(1)
Published: July 24, 2024
Commensal
microorganisms
in
the
human
gut
produce
numerous
metabolites
by
using
small
molecules
derived
from
host
or
diet
as
precursors.
Host
dietary
lipid
are
involved
energy
metabolism
and
maintaining
structural
integrity
of
cell
membranes.
Notably,
microbes
can
convert
these
lipids
into
bioactive
signaling
through
their
biotransformation
synthesis
pathways.
These
microbiota-derived
affect
physiology
influencing
body's
immune
metabolic
processes.
This
review
aims
to
summarize
recent
advances
microbial
transformation
immunomodulatory
functions
metabolites,
with
a
special
focus
on
fatty
acids
steroids
produced
our
microbiota.
ACS Omega,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 14, 2025
The
lipid
matrix
of
the
outer
membrane
(OM)
Gram-negative
bacteria
consists
a
highly
asymmetric
bilayer
containing
phospholipids
on
inner
leaflet
and
lipopolysaccharides
(LPS)
in
layer.
latter
ensures
that
harmful
molecules
do
not
permeate
bacterial
cell,
but
polymyxin
B
(PmB),
last-resort
antibiotic,
is
capable
interfering
with
stability
LPS
layer
overcoming
OM
barrier.
We
have
previously
shown
efficacy
PmB
disrupting
isotopically
models
(2H-phospholipids
1H-LPS)
regulated
by
gel-to-fluid
phase
transition
Here,
we
employ
fully
deuterated
2H-LPS)
to
track
temperature-dependent
penetration
within
model
using
neutron
reflectometry.
use
model-independent
approach
quantify
as
function
both
concentration
temperature
well
model-dependent
analysis
localize
bilayer.
By
leveraging
ability
neutrons
differentiate
hydrogen
from
deuterium
structural
biology
find
hijacks
accumulates
predominantly
hydrophobic
region
A.
Frontiers in Pharmacology,
Journal Year:
2025,
Volume and Issue:
15
Published: Jan. 14, 2025
Natural
products
are
valuable
medicinal
resources
in
the
field
of
anti-inflammation
due
to
their
significant
bioactivity
and
low
antibiotic
resistance.
Research
has
demonstrated
that
many
natural
exert
notable
anti-inflammatory
effects
by
modulating
Toll-like
receptor
4
(TLR4)
nuclear
factor
kappa
B
(NF-κB)
signaling
pathways.
The
research
on
related
signal
transduction
mechanisms
pharmacological
is
increasingly
being
discovered
validated.
However,
there
currently
a
lack
comprehensive
reviews
focusing
targeting
TLR4/NF-κB
pathway
for
effects.
In
light
these
considerations,
this
review
comprehensively
synthesizes
recent
findings
concerning
pathway,
including
translocation
TLR4
activation
lysosomes
within
cytoplasm,
assembly
protein
complexes
mediated
ubiquitin
chains
K63
K48,
deacetylation
modification
p65.
These
discoveries
integrated
into
classical
systematically
elucidate
latest
among
various
targets.
Additionally,
we
summarize
which
through
pathway.
This
aims
multitarget
advantages
treatment
inflammation
potential
applications,
thereby
providing
theoretical
support
molecular
pharmacology
development
novel
drugs.
