Proceedings of the National Academy of Sciences,
Journal Year:
2025,
Volume and Issue:
122(8)
Published: Feb. 20, 2025
Dark-field
microscopy
is
a
technique
used
in
optical
to
increase
the
contrast
unstained
samples,
making
it
possible
observe
details
that
would
otherwise
be
difficult
see
under
bright-field
microscopy;
thus,
has
been
widely
employed
biological
research,
material
science,
and
medical
diagnostics.
However,
most
dark-field
methods
cannot
overcome
diffraction
limit
require
bulky
condenser
precise
alignment
of
each
element.
In
this
study,
we
introduce
planar
photonic
device
can
produce
random
speckles
for
illumination
improve
resolution.
This
made
distribution
fibers
injection
laser
beam,
scattering
layer
speckles,
one-dimensional
crystal
(1DPC)
hollow
cone
light,
metallic
film
energy
efficiency.
work
as
substrate
conventional
microscopy.
Taking
advantage
light
with
generated
by
proposed
device,
achieve
high-contrast,
label-free
image
1.55-fold
improvement
spatial
Furthermore,
evanescent
on
1DPC
just
through
tuning
incident
wavelength,
which
demonstrates
ability
surface
imaging
beyond
limit.
The
does
not
complex
system
or
knowledge
pattern.
study
will
expand
potential
applications
provide
insights
into
samples
might
invisible
traditional
RSC Advances,
Journal Year:
2022,
Volume and Issue:
13(1), P. 80 - 114
Published: Dec. 20, 2022
In
the
field
of
targeted
drug
delivery,
effects
size
and
morphology
nanocarriers
are
great
importance
need
to
be
discussed
in
depth.
To
concise,
among
all
various
shapes
nanocarriers,
rods
tubes
with
a
narrow
cross-section
most
preferred
for
penetration
cell
membrane.
this
regard,
several
studies
have
focused
on
methods
produce
nanorods
nanotubes
controlled
optimized
aspect
ratio
(AR).
Additionally,
non-spherical
orientation
could
affect
cellular
uptake
process
while
tangent
angle
less
than
45°
is
better
at
penetrating
membrane,
Nature Methods,
Journal Year:
2023,
Volume and Issue:
20(3), P. 442 - 447
Published: Feb. 27, 2023
Abstract
Interferometric
scattering
(iSCAT)
microscopy
is
a
label-free
optical
method
capable
of
detecting
single
proteins,
localizing
their
binding
positions
with
nanometer
precision,
and
measuring
mass.
In
the
ideal
case,
iSCAT
limited
by
shot
noise
such
that
collection
more
photons
should
extend
its
detection
sensitivity
to
biomolecules
arbitrarily
low
However,
number
technical
sources
combined
speckle-like
background
fluctuations
have
restricted
limit
in
iSCAT.
Here,
we
show
an
unsupervised
machine
learning
isolation
forest
algorithm
for
anomaly
pushes
mass
factor
4
below
10
kDa.
We
implement
this
scheme
both
user-defined
feature
matrix
self-supervised
FastDVDNet
validate
our
results
correlative
fluorescence
images
recorded
total
internal
reflection
mode.
Our
work
opens
door
investigations
small
traces
disease
markers
as
α-synuclein,
chemokines
cytokines.
ACS Physical Chemistry Au,
Journal Year:
2023,
Volume and Issue:
3(2), P. 143 - 156
Published: Jan. 12, 2023
In
recent
years,
the
sensitivity
and
specificity
of
optical
sensors
has
improved
tremendously
due
to
improvements
in
biochemical
functionalization
protocols
detection
systems.
As
a
result,
single-molecule
been
reported
range
biosensing
assay
formats.
this
Perspective,
we
summarize
that
achieve
direct
label-free
assays,
sandwich
competitive
assays.
We
describe
advantages
disadvantages
assays
future
challenges
field
including
their
miniaturization
integration,
multimodal
sensing
capabilities,
accessible
time
scales,
compatibility
with
real-life
matrices
such
as
biological
fluids.
conclude
by
highlighting
possible
application
areas
include
not
only
healthcare
but
also
monitoring
environment
industrial
processes.
Annual Review of Physical Chemistry,
Journal Year:
2024,
Volume and Issue:
75(1), P. 163 - 183
Published: Feb. 16, 2024
By
superlocalizing
the
positions
of
millions
single
molecules
over
many
camera
frames,
a
class
super-resolution
fluorescence
microscopy
methods
known
as
single-molecule
localization
(SMLM)
has
revolutionized
how
we
understand
subcellular
structures
past
decade.
In
this
review,
highlight
emerging
studies
that
transcend
outstanding
structural
(shape)
information
offered
by
SMLM
to
extract
and
map
physicochemical
parameters
in
living
mammalian
cells
at
levels.
encoding/decoding
high-dimensional
information-such
emission
excitation
spectra,
motion,
polarization,
lifetime,
beyond-for
every
molecule,
mass
accumulating
these
measurements
for
molecules,
such
multidimensional
multifunctional
approaches
open
new
windows
into
intracellular
architectures
dynamics,
well
their
underlying
biophysical
rules,
far
beyond
diffraction
limit.
The Journal of Chemical Physics,
Journal Year:
2022,
Volume and Issue:
156(16)
Published: April 6, 2022
We
review
some
of
the
progress
single-molecule
optical
experiments
in
past
20
years
and
propose
perspectives
for
coming
years.
particularly
focus
on
methodological
advances
fluorescence,
super-resolution,
photothermal
contrast,
interferometric
scattering
briefly
discuss
a
few
applications.
These
have
enabled
exploration
new
emitters
quantum
optics;
chemistry
biology
complex
heterogeneous
systems,
nanoparticles,
plasmonics;
detection
study
non-fluorescing
non-absorbing
nano-objects.
conclude
by
proposing
ideas
future
experiments.
The
field
will
move
toward
more
better
signals
broader
variety
objects
sharper
view
surprising
complexity
nanoscale
world
single
(bio-)molecules,
their
nano-environments.
