Two-color interferometric scattering (iSCAT) microscopy reveals structural dynamics in discrete plasmonic molecules DOI Creative Commons

Leslie Velasco,

Aniqa N. Islam,

Koustav Kundu

et al.

Nanoscale, Journal Year: 2024, Volume and Issue: 16(24), P. 11696 - 11704

Published: Jan. 1, 2024

Plasmonic molecules are discrete assemblies of noble metal nanoparticles (NPs) that interest as transducers in optical nanosensors. So far, NPs with diameters ∼40 nm have been the preferred building blocks for plasmonic intended single molecule sensors due to difficulties associated detecting smaller through elastic scattering conventional darkfield microscopy. Here, we apply 405 nm, 445 two-color interferometric (iSCAT) microscopy characterize polyethylene glycol (PEG) tethered dimers 10 and 20 Ag their monomers. Dimers both NP sizes can be discerned from respective monomers changes average iSCAT contrast. In case NPs, dimer formation induces a change sign contrast, providing characteristic signal binding events. 0.4 kDa 3.4 spacers show contrast distributions significantly different averages on wavelength channels. The measured individual PEG-tethered or function time shows fluctuations indicative rich structural dynamics assembled molecules, which provides an additional metric discern paves path new class plasmon rulers.

Language: Английский

Spatial redundancy transformer for self-supervised fluorescence image denoising DOI Creative Commons
Xinyang Li, Xiaowan Hu, Xingye Chen

et al.

Nature Computational Science, Journal Year: 2023, Volume and Issue: 3(12), P. 1067 - 1080

Published: Dec. 11, 2023

Abstract Fluorescence imaging with high signal-to-noise ratios has become the foundation of accurate visualization and analysis biological phenomena. However, inevitable noise poses a formidable challenge to sensitivity. Here we provide spatial redundancy denoising transformer (SRDTrans) remove from fluorescence images in self-supervised manner. First, sampling strategy based on is proposed extract adjacent orthogonal training pairs, which eliminates dependence speed. Second, designed lightweight spatiotemporal architecture capture long-range dependencies high-resolution features at low computational cost. SRDTrans can restore high-frequency information without producing oversmoothed structures distorted traces. Finally, demonstrate state-of-the-art performance single-molecule localization microscopy two-photon volumetric calcium imaging. does not contain any assumptions about process sample, thus be easily extended various modalities applications.

Language: Английский

Citations

24
Surface Plasmon Resonance Biosensors: A Review of Molecular Imaging with High Spatial Resolution DOI Creative Commons
Jiying Xu, Pengfei Zhang,

Yi Chen

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(2), P. 84 - 84

Published: Feb. 2, 2024

Surface plasmon resonance (SPR) is a powerful tool for determining molecular interactions quantitatively. SPR imaging (SPRi) further improves the throughput of technology and provides spatially resolved capability observing interaction dynamics in detail. SPRi becoming more popular biological chemical sensing imaging. However, suffers from low spatial resolution due to imperfect optical components delocalized features propagating surface plasmonic waves along surface. Diverse kinds approaches have been developed improve SPRi, which enormously impelled development methodology extended its possible applications. In this minireview, we introduce mechanisms building high-spatial-resolution system present experimental schemes prism-coupled microscopy (SPRM) scattering (SPSM); summarize exciting applications, including analysis, profiling, tracking single entities, analysis cells; discuss challenges recent decade as well promising future.

Language: Английский

Citations

13
Single-Molecule Spectroscopy and Super-Resolution Mapping of Physicochemical Parameters in Living Cells DOI
Megan A. Steves, Changdong He, Ke Xu

et al.

Annual Review of Physical Chemistry, Journal Year: 2024, Volume and Issue: 75(1), P. 163 - 183

Published: Feb. 16, 2024

By superlocalizing the positions of millions single molecules over many camera frames, a class super-resolution fluorescence microscopy methods known as single-molecule localization (SMLM) has revolutionized how we understand subcellular structures past decade. In this review, highlight emerging studies that transcend outstanding structural (shape) information offered by SMLM to extract and map physicochemical parameters in living mammalian cells at levels. encoding/decoding high-dimensional information-such emission excitation spectra, motion, polarization, lifetime, beyond-for every molecule, mass accumulating these measurements for molecules, such multidimensional multifunctional approaches open new windows into intracellular architectures dynamics, well their underlying biophysical rules, far beyond diffraction limit.

Language: Английский

Citations

12
Label-free detection and profiling of individual solution-phase molecules DOI
Lisa-Maria Needham, Carlos Saavedra, Julia K. Rasch

et al.

Nature, Journal Year: 2024, Volume and Issue: 629(8014), P. 1062 - 1068

Published: May 8, 2024

Language: Английский

Citations

12
Label-Free Imaging of DNA Interactions with 2D Materials DOI Creative Commons
Jenny Sülzle, Wayne Yang,

Yuta Shimoda

et al.

ACS Photonics, Journal Year: 2024, Volume and Issue: 11(2), P. 737 - 744

Published: Jan. 10, 2024

Two-dimensional (2D) materials offer potential as substrates for biosensing devices, their properties can be engineered to tune interactions between the surface and biomolecules. Yet, not many methods measure these in a liquid environment without introducing labeling agents such fluorophores. In this work, we harness interferometric scattering (iSCAT) microscopy, label-free imaging technique, investigate of single molecules long dsDNA with 2D materials. The millisecond temporal resolution iSCAT allows us capture transient observe dynamics unlabeled DNA binding hexagonal boron nitride (hBN) solution extended periods (including fraction 10%, trajectories lasting longer than 110 ms). Using focused ion beam technique engineer defects, find that affinity is enhanced at defects; when exposed lanes, binds preferentially lane edges. Overall, demonstrate useful tool study how biomolecules interact materials, key component engineering future biosensors.

Language: Английский

Citations

9
Molecular fingerprinting of biological nanoparticles with a label-free optofluidic platform DOI Creative Commons
Alexia Stollmann, Jose Garcia‐Guirado, Jae‐Sang Hong

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 15, 2024

Label-free detection of multiple analytes in a high-throughput fashion has been one the long-sought goals biosensing applications. Yet, for all-optical approaches, interfacing state-of-the-art label-free techniques with microfluidics tools that can process small volumes sample high throughput, and surface chemistry grants analyte specificity, poses critical challenge to date. Here, we introduce an optofluidic platform brings together digital holography PDMS by using supported lipid bilayers as building block integrate both technologies. Specifically, this fingerprints heterogeneous biological nanoparticle populations via multiplexed immunoaffinity assay single particle sensitivity. First, characterise robustness performance platform, then apply it profile four distinct ovarian cell-derived extracellular vesicle over panel protein biomarkers, thus developing unique biomarker fingerprint each cell line. We foresee our approach will find many applications where routine characterisation nanoparticles are required.

Language: Английский

Citations

8
Single-molecule FRET for probing nanoscale biomolecular dynamics DOI
Daniel Nettels, Nicola Galvanetto, Miloš T. Ivanović

et al.

Nature Reviews Physics, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 12, 2024

Citations

7
Dual-Angle Interferometric Scattering Microscopy for Optical Multiparametric Particle Characterization DOI Creative Commons
Erik Olsén,

Berenice Rodríguez,

Fredrik Skärberg

et al.

Nano Letters, Journal Year: 2024, Volume and Issue: 24(6), P. 1874 - 1881

Published: Jan. 31, 2024

Traditional single-nanoparticle sizing using optical microscopy techniques assesses size via the diffusion constant, which requires suspended particles to be in a medium of known viscosity. However, these assumptions are typically not fulfilled complex natural sample environments. Here, we introduce dual-angle interferometric scattering (DAISY), enabling quantification both and polarizability individual nanoparticles (radius <170 nm) without requiring priori information regarding surrounding media or super-resolution imaging. DAISY achieves this by combining contained concurrently measured forward backward images through twilight off-axis holography (iSCAT). Going beyond particle polarizability, single-particle morphology can deduced from fact that hydrodynamic radius relates outer radius, while scattering-based estimate depends on internal mass distribution particles. We demonstrate differentiating biomolecular fractal aggregates spherical fetal bovine serum at level.

Language: Английский

Citations

6
Interferometric Phase Intensity Nanoscopy (iPINE), Revealing Nanostructural Features, Resolves Proximal Nanometer Objects below the Diffraction Limit: Implications in Long-Time Super-Resolution of Biological Dynamics DOI
Guangjie Cui, Do Young Kim, Di Zu

et al.

ACS Applied Nano Materials, Journal Year: 2024, Volume and Issue: 7(16), P. 18443 - 18449

Published: Feb. 12, 2024

The ability to spatially and temporally map nanoscale environments in situ over extended time scales would be transformative for biology, biomedicine, bioengineering. All nanometer objects, from nanoparticles down single proteins, scatter light. Interferometric scattering stands as a powerful tool, offering ultrasensitivity resolution vital visualizing entities. an individual nanoparticle protein has been detected; however, resolving adjacent objects with interferometric not yet demonstrated. In this work, we present phase intensity nanoscopy (iPINE) resolve scattering. We demonstrate that multiphase sensitivity of iPINE reveal ellipse Airy patterns correlated nanostructural features. show eliminating background fluctuation by employing circularly polarized illumination is essential separate proximal below the diffraction limit. envision variety proteins periods wide range applications expect especially important biological dynamics require observation times.

Language: Английский

Citations

5
iSCAT microscopy and particle tracking with tailored spatial coherence DOI Creative Commons
Mahdi Mazaheri,

Kiarash Kasaian,

David Albrecht

et al.

Optica, Journal Year: 2024, Volume and Issue: 11(7), P. 1030 - 1030

Published: June 21, 2024

Interferometric scattering (iSCAT) microscopy has demonstrated unparalleled performance among label-free optical methods for detecting and imaging isolated nanoparticles molecules. However, when complex structures such as biological cells, the superposition of fields from different locations sample leads to a speckle-like background, posing significant challenge in deciphering fine features. Here, we show that by controlling spatial coherence illumination, one can eliminate spurious speckle without sacrificing sensitivity. We demonstrate this approach positioning rotating diffuser coupled with an adjustable lens iris illumination path. report on at high frame rate 25 kHz across large field view 100µm×100µm, while maintaining diffraction-limited resolution. showcase advantages these features three-dimensional (3D) tracking over 1000 vesicles single COS-7 cell dynamics endoplasmic reticulum (ER) network. Our opens door combination imaging, sensitive detection, 3D high-speed using wide-field iSCAT microscopy.

Language: Английский

Citations

5