Direct tests of cytochrome c and c 1 functions in the electron transport chain of malaria parasites DOI Creative Commons
Tanya J. Espino-Sanchez, Henry Wienkers,

Rebecca G. Marvin

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(19)

Published: May 1, 2023

The mitochondrial electron transport chain (ETC) of Plasmodium malaria parasites is a major antimalarial drug target, but critical cytochrome (cyt) functions remain unstudied and enigmatic. Parasites express two distinct cyt c homologs (c c-2) with unusually sparse sequence identity uncertain fitness contributions. P. falciparum c-2 the most divergent eukaryotic homolog currently known has features predicted to be incompatible canonical ETC function. We tagged both related c1 for inducible knockdown. Translational repression was lethal parasites, which died from dysfunction impaired ubiquinone recycling. In contrast, knockdown or knockout had little impact on blood-stage growth, indicating that rely fully more conserved Biochemical structural studies revealed are hemylated by holocytochrome synthase, UV-vis absorbance EPR spectra strongly suggest an open active site in heme stably coordinated only single axial amino acid ligand can bind exogenous small molecules. These provide direct dissection identify highly molecular adaptations defy role evolution.

Language: Английский

Smart Plasmonic Nanorobot for Real-Time Monitoring Cytochrome c Release and Cell Acidification in Apoptosis during Electrostimulation DOI
Guohua Qi, Haijuan Li, Ying Zhang

et al.

Analytical Chemistry, Journal Year: 2018, Volume and Issue: 91(2), P. 1408 - 1415

Published: Nov. 20, 2018

Cytochrome c (Cyt c) release and cellular pH change are two important mediators of apoptosis. Effective methods to regulate or monitor such events therefore highly desired for apoptosis research cancer cell therapy. Herein, we exploited electrostimulation Cyt process, by designing preparing a smart efficient plasmonic nanorobot (with surface-modified c-specific aptamer 4-mercaptobenzoic acid) that is capable capture self-sensing, achieved real-time SERS monitoring dynamic simultaneous acidification in during electrostimulation. Distinctly different molecular stress responses the cancerous MCF-7 HeLa cells normal L929 were identified revealed. The method results valuable promising c-mediated biology studies.

Language: Английский

Citations

57
From iron to bacterial electroconductive filaments: Exploring cytochrome diversity using Geobacter bacteria DOI
Carlos A. Salgueiro, Leonor Morgado, Marta A. Silva

et al.

Coordination Chemistry Reviews, Journal Year: 2021, Volume and Issue: 452, P. 214284 - 214284

Published: Nov. 15, 2021

Language: Английский

Citations

36
Biomolecule-Compatible Dehydrogenative Chan–Lam Coupling of Free Sulfilimines DOI
Tingting Meng, Lucille A. Wells, Tianxin Wang

et al.

Journal of the American Chemical Society, Journal Year: 2022, Volume and Issue: 144(27), P. 12476 - 12487

Published: June 29, 2022

Inspired by the discovery of a S═N bond in collagen IV network and its essential role stabilizing basement membranes, sulfilimines have drawn much attention fields chemistry biology. However, their further uptake is hindered lack mild, efficient, environmentally benign protocols which can be constructed under biomolecule-compatible conditions. Here, we report terminal oxidant-free copper-catalyzed dehydrogenative Chan–Lam coupling free diaryl with arylboronic acids excellent chemoselectivity broad substrate compatibility. The mild reaction conditions nature allow employment this protocol late-stage functionalization complex peptides, more importantly, as an effective bioconjugation method showcased model protein. A combined experimental computational mechanistic investigation reveals that inner-sphere electron-transfer process circumvents sacrificial oxidant employed traditional reactions. An energetically viable concerted pathway was located wherein copper hydride facilitates hydrogen-atom abstraction from isopropanol solvent to produce dihydrogen via four-membered transition state.

Language: Английский

Citations

28
Raman Spectroscopy of Oral Candida Species: Molecular-Scale Analyses, Chemometrics, and Barcode Identification DOI Open Access
Giuseppe Pezzotti, Miyuki Kobara,

Tamaki Nakaya

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(10), P. 5359 - 5359

Published: May 11, 2022

Oral candidiasis, a common opportunistic infection of the oral cavity, is mainly caused by following four

Language: Английский

Citations

24
Direct tests of cytochrome c and c 1 functions in the electron transport chain of malaria parasites DOI Creative Commons
Tanya J. Espino-Sanchez, Henry Wienkers,

Rebecca G. Marvin

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(19)

Published: May 1, 2023

The mitochondrial electron transport chain (ETC) of Plasmodium malaria parasites is a major antimalarial drug target, but critical cytochrome (cyt) functions remain unstudied and enigmatic. Parasites express two distinct cyt c homologs (c c-2) with unusually sparse sequence identity uncertain fitness contributions. P. falciparum c-2 the most divergent eukaryotic homolog currently known has features predicted to be incompatible canonical ETC function. We tagged both related c1 for inducible knockdown. Translational repression was lethal parasites, which died from dysfunction impaired ubiquinone recycling. In contrast, knockdown or knockout had little impact on blood-stage growth, indicating that rely fully more conserved Biochemical structural studies revealed are hemylated by holocytochrome synthase, UV-vis absorbance EPR spectra strongly suggest an open active site in heme stably coordinated only single axial amino acid ligand can bind exogenous small molecules. These provide direct dissection identify highly molecular adaptations defy role evolution.

Language: Английский

Citations

14