A critical review of pollution active biomonitoring using sentinel fish: challenges and opportunities

Environmental Pollution, Journal Year: 2024, Volume and Issue: 360, P. 124661 - 124661

Published: Aug. 5, 2024

Language: Английский

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Effect-based evaluation of water quality in a system of indirect reuse of wastewater for drinking water production

Water Research, Journal Year: 2023, Volume and Issue: 242, P. 120147 - 120147

Published: May 31, 2023

Indirect potable reuse of wastewater is a practice that gaining attention, aiming to increase freshwater supplies meet water scarcity. However, reusing effluent for drinking production comes with paired risk adverse health effects, due the potential presence pathogenic microorganisms and hazardous micropollutants. Disinfection an established method reduce microbial hazards in water, but it has been associated formation disinfection by-products (DBPs). In this study, we performed effect-based assessment chemical system wherein full-scale trial by chlorination, treated was prior discharge reciepient river. The bioactive pollutants assessed along entire treatment system, starting from incoming finished at seven sites around Llobregat River Barcelona, Spain. Samples were collected two campaigns, without applied chlorination (13 mg Cl2/L) wastewater. samples analysed cell viability, oxidative stress response (Nrf2 activity), estrogenicity, androgenicity, aryl hydrocarbon receptor (AhR) activity activation NFĸB (nuclear factor kappa-light-chain-enhancer activated B cells) signaling using stably transfected mammalian lines. Nrf2 activity, estrogen AhR detected all investigated samples. Overall, removal efficiencies high both most studied endpoints. No activity) could be attributed additional found reduction ER agonistic after bioactivity considerably lower compared what We thus conclude indirect can possible compromising quality. This study contributed important knowledge efforts as source production.

Language: Английский

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Global occurrence of synthetic glucocorticoids and glucocorticoid receptor agonistic activity, and aquatic hazards in effluent discharges and freshwater systems

Environmental Pollution, Journal Year: 2023, Volume and Issue: 329, P. 121638 - 121638

Published: April 18, 2023

Language: Английский

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Comparative Glucocorticoid Receptor Agonism: In Silico, In Vitro, and In Vivo and Identification of Potential Biomarkers for Synthetic Glucocorticoid Exposure

Environmental Toxicology and Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

Abstract The glucocorticoid receptor (GR) is present in almost every vertebrate cell and utilized many biological processes. Despite an abundance of mammalian data, the structural conservation cross-species susceptibility, particularly for aquatic species, has not been well defined. Efforts to reduce, refine, and/or replace animal testing have increased, driving impetus advance development new approach methodologies (NAMs). Here we used silico, vitro, vivo methods elucidate a greater understanding receptor-mediated effects synthetic exposure teleost fish. Evolutionary amino acid residues critical transcriptional activation was confirmed silico using sequence alignment predict across species susceptibility. Subsequent vitro assays zebrafish human GR provided evidence physiological congruence agonism. Finally, adult fathead minnows (Pimephales promelas) were exposed glucocorticoids, dexamethasone (0.04, 400, 4,000 µg/L) beclomethasone dipropionate (130 µg/L), agonism via digital polymerase chain reaction; addition, EcoToxChip analyses identified potential mRNA biomarkers following exposure. These findings support use NAMs potentially reduce multispecies experimentation while providing empirical that expands taxonomic domain applicability molecular initiating event within broader adverse outcome pathway network.

Language: Английский

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Inhibition of Fin Regeneration in Fathead Minnow (Pimephales promelas) by a Potent Synthetic Glucocorticoid and Development of Adverse Outcome Pathway 334

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: May 6, 2025

Despite structural and functional conservation across vertebrate species, the glucocorticoid receptor has been minimally studied in comparison to other biological targets for endocrine-disrupting compounds aquatic systems. Because prolonged use of pharmaceutical glucocorticoids humans linked osteoporosis impaired bone growth, we hypothesized that ability teleost fish regenerate fins following damage may be inhibited by exposure synthetic environment. In present study, examined fin regeneration a 7 days waterborne juvenile fathead minnows (Pimephales promelas) glucocorticoids, fluticasone propionate dexamethasone. Expression several biologically relevant gene products (sgk1, tdgf1, runx2a, lef1, shha, tsc22d3) was measured paired caudal whole-body tissues. Fluticasone dexamethasone significantly at water concentrations 2.62 μg/L 4.62 mg/L, respectively. Changes expression indicated disruption intercellular communication Wnt/β-catenin morphogenetic protein (BMP) signaling pathways after 4.86 propionate. Upregulation tsc22d3, transcription factor responsible suppression anti-inflammatory response, plausible cause repressed cellular signaling. These findings advance development adverse outcome pathway 334─Glucocorticoid Receptor Activation Leads Impaired Fin Regeneration─and elucidate both mechanistic relationship between activation inhibition regeneration, which could plausibly reduce individual fitness

Language: Английский

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Food, Beverage, and Feedstock Processing Facility Wastewater: a Unique and Underappreciated Source of Contaminants to U.S. Streams

Environmental Science & Technology, Journal Year: 2021, Volume and Issue: 56(2), P. 1028 - 1040

Published: Dec. 30, 2021

Process wastewaters from food, beverage, and feedstock facilities, although regulated, are an under-investigated environmental contaminant source. Food process (FPWWs) 23 facilities in 17 U.S. states were sampled documented for a plethora of chemical microbial contaminants. Of the 576 analyzed organics, 184 (32%) detected at least once, with concentrations as large 143 μg L–1 (6:2 fluorotelomer sulfonic acid), many 47 single FPWW sample. Cumulative per/polyfluoroalkyl substance up to 185 pesticide transformation product (e.g., methomyl oxime, 40 L–1; clothianidin TMG, 2.02 L–1) observed. Despite 48% undergoing disinfection treatment prior discharge, bacteria resistant third-generation antibiotics found each facility type, multiple bacterial groups all samples, including total coliforms. The exposure–activity ratios toxicity quotients exceeded 1.0 13 22% respectively, indicating potential biological effects vertebrates invertebrates associated discharge FPWW. Organic profiles differed previously reported municipal effluents urban storm water, that is another important source mixtures discharged into receiving surface waters.

Language: Английский

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Effect-based monitoring to integrate the mixture hazards of chemicals into water safety plans

Journal of Water and Health, Journal Year: 2022, Volume and Issue: 20(12), P. 1721 - 1732

Published: Nov. 16, 2022

Water safety plans (WSPs) are intended to assure safe drinking water (DW). WSPs involve assessing and managing risks associated with microbial, chemical, physical radiological hazards from the catchment consumer. Currently, chemical in assessed by targeted analysis, but this approach fails account for mixture effects of many chemicals potentially present supplies omits possible non-targeted chemicals. Consequently, effect-based monitoring (EBM) using vitro bioassays well plate-based vivo assays proposed as a complementary tool analysis support risk management quality verification within WSP framework. EBM is frequently applied DW surface can be utilised all defined categories framework (including 'system assessment', 'validation', 'operational' 'verification'). Examples how different modules provided, along guidance on where apply frequently. Since new area, documents, standard operating procedures (SOPs) decision-making frameworks required both bioassay operators teams facilitate integration into WSPs, these resources being developed currently.

Language: Английский

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Evaluation of Complex Mixture Toxicity in the Milwaukee Estuary (WI, USA) Using Whole‐Mixture and Component‐Based Evaluation Methods

Environmental Toxicology and Chemistry, Journal Year: 2023, Volume and Issue: 42(6), P. 1229 - 1256

Published: Jan. 30, 2023

Abstract Anthropogenic activities introduce complex mixtures into aquatic environments, necessitating mixture toxicity evaluation during risk assessment. There are many alternative approaches that can be used to complement traditional techniques for Our study aimed demonstrate how these could employed in a target watershed. Evaluations were carried out over 2 years (2017–2018) across 8–11 sites the Milwaukee Estuary (WI, USA). Whole evaluated on site‐specific basis by deploying caged fathead minnows ( Pimephales promelas ) alongside composite samplers 96 h and characterizing chemical composition, vitro bioactivity of collected water samples, vivo effects whole organisms. Chemicals grouped based structure/mode action, bioactivity, pharmacological activity. Priority chemicals identified their relative contributions estimated pressure (based cumulative toxic units) via predictive assessments (random forest regression). further including two targeted industrial/urban assessment; three pharmaceutical characterization; low‐priority sites. Analyses 14 16 significantly contributed effects, representing high or medium priority targets ecotoxicological evaluation, monitoring, regulatory Overall, our represents an important single‐chemical prioritizations, providing comprehensive detected Furthermore, it demonstrates different tools identify diverse facets highlights strategies considered future assessments. Environ Toxicol Chem 2023;42:1229–1256. © 2023 SETAC

Language: Английский

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Recognition and Selectivity Analysis Monitoring of Multicomponent Steroid Estrogen Mixtures in Complex Systems Using a Group-Targeting Environmental Sensor

Environmental Science & Technology, Journal Year: 2021, Volume and Issue: 55(20), P. 14115 - 14125

Published: Aug. 30, 2021

The same class of steroid estrogen mixtures, coexisting in the environment 17β-estradiol, estrone (E1), and ethinyl estradiol (EE2), have strong ability to disrupt human endocrine system are seriously prejudicial health organism environmental safety. Herein, a highly sensitive group-targeting monitoring sensor was fabricated for comprehensive analysis multicomponent estrogens (multi-SEs) complex systems. This breakthrough based on photoelectrochemical response composite material CdSe NPs-TiO2 nanotube group-specific aptamers. optimized procedure exhibited not only high sensitivity wide range concentrations from 0.1 50 nM, indeed, minimum detection limit 33 pM, but also resistance interference. affinity consistent action pockets this enable selective multi-SEs It subsequently applied three real samples including medical wastewater, river water, tap water provide means clarify fate process migration transformation. has brilliant application prospect identification endocrine-disrupting chemical mixtures

Language: Английский

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Effects-Based Monitoring of Bioactive Chemicals Discharged to the Colorado River before and after a Municipal Wastewater Treatment Plant Replacement

Environmental Science & Technology, Journal Year: 2020, Volume and Issue: 55(2), P. 974 - 984

Published: Dec. 29, 2020

Monitoring of the Colorado River near Moab, Utah, wastewater treatment plant (WWTP) outflow has detected pharmaceuticals, hormones, and estrogen-receptor (ER)-, glucocorticoid receptor (GR)-, peroxisome proliferator-activated receptor-gamma (PPARγ)-mediated biological activities. The aim present multi-year study was to assess effects a WWTP replacement on bioactive chemical (BC) concentrations. Water samples were collected bimonthly, pre- post-replacement, at 11 sites along upstream downstream analyzed for in vitro bioactivities (e.g., agonism ER, GR, PPARγ) BC concentrations; fathead minnows cage deployed post-replacement with varying proximities WWTP. Before replacement, ER (24 ng 17β-estradiol equivalents/L)-, GR (60 dexamethasone PPARγ-mediated activities but diminished downstream. In March 2018, effluent acutely toxic fish, likely due elevated ammonia Following PPARγ reduced by approximately 60–79%, no toxicity observed caged there marked decreases concentrations many BCs. Results suggest that Moab achieved significant reduction River.

Language: Английский

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