Water Research,
Journal Year:
2024,
Volume and Issue:
261, P. 122024 - 122024
Published: June 30, 2024
Water
quality,
critical
for
human
survival
and
well-being,
necessitates
rigorous
control
to
mitigate
contamination
risks,
particularly
from
pathogens
amid
expanding
urbanization.
Consequently,
the
necessity
maintain
microbiological
safety
of
water
supplies
demands
effective
surveillance
strategies,
reliant
on
collection
representative
samples
precise
measurement
contaminants.
This
review
critically
examines
advancements
passive
sampling
techniques
monitoring
in
various
systems,
including
wastewater,
freshwater,
seawater.
We
explore
evolution
conventional
materials
innovative
adsorbents
pathogen
capture
shift
culture-based
molecular
detection
methods,
underscoring
adaptation
this
field
global
health
challenges.
The
comparison
highlights
sampling's
efficacy
over
like
grab
its
potential
overcome
existing
challenges
through
use
such
as
granular
activated
carbon,
thermoplastics,
polymer
membranes.
By
evaluating
literature,
work
identifies
standardization
gaps
proposes
future
research
directions
augment
efficiency,
specificity,
utility
environmental
public
surveillance.
Journal of Water and Health,
Journal Year:
2023,
Volume and Issue:
21(6), P. 831 - 848
Published: May 16, 2023
Concentrations
of
nucleic
acids
from
a
range
respiratory
viruses
in
wastewater
solids
collected
treatment
plants
correlate
to
clinical
data
on
disease
occurrence
the
community
contributing
wastewater.
Viral
enter
excretions
deposited
toilets
or
drains.
To
relate
measured
concentrations
at
plant
number
infections,
viral
nucleic-acid
human
are
needed
as
inputs
mass
balance
model.
Here,
we
carried
out
systematic
review
and
meta-analysis
characterize
presence
influenza
A
B,
syncytial
virus,
metapneumovirus,
parainfluenza
rhinovirus,
seasonal
coronaviruses
stool,
urine,
mucus,
sputum,
saliva.
We
identified
220
sets
50
articles
reported
these
excretions.
Data
were
unevenly
distributed
across
virus
type
(with
most
available
for
influenza)
excretion
excretions).
Most
only
absence
cross-sectional
study
design.
There
is
need
more
concentration
data,
including
longitudinal
all
types.
Such
would
allow
quantitatively
linking
numbers
infected
individuals.
Environmental Science & Technology Letters,
Journal Year:
2023,
Volume and Issue:
11(1), P. 9 - 15
Published: Dec. 11, 2023
Climate
change
and
urbanization
are
increasing
the
distribution
of
insect
vectors
infectious
diseases.
Dengue
virus
is
an
arbovirus
that
causes
nearly
100
million
symptomatic
infections
per
year
endemic
in
124
countries,
range
its
mosquito
continues
to
increase.
Surveillance
dengue
complicated
by
fact
can
be
asymptomatic,
symptoms
may
not
readily
recognizable
clinicians.
Here
we
show
wastewater
monitoring
used
detect
RNA
yield
information
about
circulation
a
community.
We
collected
three
samples
solids
week
from
different
treatment
plants
Miami-Dade
County,
Florida,
where
were
locally
acquired.
Using
molecular
methods,
tested
for
4
serotypes
consistently
detected
3
at
all
did
other
serotypes.
According
publicly
available
data
on
infections,
vast
majority
caused
serotype
3.
Wastewater
detection
possible
with
as
few
4.23
laboratory
confirmed
cases
1
people
based
infection
data.
Biomedical Engineering Advances,
Journal Year:
2023,
Volume and Issue:
6, P. 100092 - 100092
Published: May 18, 2023
Digital
polymerase
chain
reaction
(dPCR)
is
an
emerging
technique
for
the
absolute
quantification
of
target
nucleic
acids.
dPCR
got
attention
as
a
precise
tool
in
preclinical
research,
particularly
when
used
to
detect
genetic
mutations
and
result
highly
measurements.
In
dPCR,
statistic
Poisson
distribution
was
followed
random
molecules
different
partitions,
which
essential
quantification.
Amplified
sequences
partitions
are
identified
by
fluorescence
each
partition
functions
separate
PCR
microreactor.
Without
need
calibration,
percentage
PCR-positive
sufficient
estimate
concentration
sequence.
The
present
revolution
digital
made
possible
advancements
microfluidics,
provided
effective
partitioning
techniques.
this
paper,
contrast
underlying
ideas
quantitative
real-time
with
measurement
acids
quantity
Polymerase
(q-PCR).
This
review
study
briefly
introduced
background
compared
types
PCR,
qPCR
PCR.
fundamental
concept
also
explained
compares
advantages
over
analyzes
applications
diagnostic
cancer
viral
species.
mSphere,
Journal Year:
2024,
Volume and Issue:
9(3)
Published: Feb. 27, 2024
Enteric
infections
are
important
causes
of
morbidity
and
mortality,
yet
clinical
surveillance
is
limited.
Wastewater-based
epidemiology
(WBE)
has
been
used
to
study
community
circulation
individual
enteric
viruses
panels
respiratory
diseases,
but
there
limited
work
studying
the
concurrent
a
suite
viruses.
A
retrospective
WBE
was
carried
out
at
two
wastewater
treatment
plants
located
in
California,
United
States.
Using
digital
droplet
polymerase
chain
reaction
(PCR),
we
measured
concentrations
human
adenovirus
group
F,
enteroviruses,
norovirus
genogroups
I
II,
rotavirus
nucleic
acids
solids
times
per
week
for
26
months
(
Water Research,
Journal Year:
2024,
Volume and Issue:
261, P. 122024 - 122024
Published: June 30, 2024
Water
quality,
critical
for
human
survival
and
well-being,
necessitates
rigorous
control
to
mitigate
contamination
risks,
particularly
from
pathogens
amid
expanding
urbanization.
Consequently,
the
necessity
maintain
microbiological
safety
of
water
supplies
demands
effective
surveillance
strategies,
reliant
on
collection
representative
samples
precise
measurement
contaminants.
This
review
critically
examines
advancements
passive
sampling
techniques
monitoring
in
various
systems,
including
wastewater,
freshwater,
seawater.
We
explore
evolution
conventional
materials
innovative
adsorbents
pathogen
capture
shift
culture-based
molecular
detection
methods,
underscoring
adaptation
this
field
global
health
challenges.
The
comparison
highlights
sampling's
efficacy
over
like
grab
its
potential
overcome
existing
challenges
through
use
such
as
granular
activated
carbon,
thermoplastics,
polymer
membranes.
By
evaluating
literature,
work
identifies
standardization
gaps
proposes
future
research
directions
augment
efficiency,
specificity,
utility
environmental
public
surveillance.