medRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Abstract
In
2024,
the
highly
pathogenic
avian
influenza
A
H5N1
caused
outbreaks
in
wild
birds,
poultry,
cows,
and
other
mammals
United
States
with
61
human
cases
also
reported
by
CDC.
Detection
of
H5
RNA
wastewater
has
been
previously
sewersheds
Texas
North
Carolina
nearby
impacted
dairy
herds
following
emergence
cows.
Here,
we
conduct
retrospective
testing
total
hemagglutinin
genes
as
well
presenting
applying
new
assays
for
detection
H1
H3
across
a
respiratory
virus
season
an
urban
California
sewershed
from
September
2023
–
May
2024.
Total
A,
H1,
were
regularly
detected,
while
was
first
detected
March.
We
developed
model
that
uses
Monte
Carlo
simulations
published
parameters
to
estimate
numbers
infected
people,
or
liters
H5-contaminated
milk
required
result
measured
concentrations
wastewater.
Our
findings
demonstrate
this
sewershed,
contaminated
poultry
most
likely
sources
created
publicly
available
tool
apply
input
inputs.
Synopsis
understand
potential
wastewater,
enabling
interpretation
detections.
TOC
figure
Journal of Water and Health,
Journal Year:
2023,
Volume and Issue:
21(6), P. 831 - 848
Published: May 16, 2023
Concentrations
of
nucleic
acids
from
a
range
respiratory
viruses
in
wastewater
solids
collected
treatment
plants
correlate
to
clinical
data
on
disease
occurrence
the
community
contributing
wastewater.
Viral
enter
excretions
deposited
toilets
or
drains.
To
relate
measured
concentrations
at
plant
number
infections,
viral
nucleic-acid
human
are
needed
as
inputs
mass
balance
model.
Here,
we
carried
out
systematic
review
and
meta-analysis
characterize
presence
influenza
A
B,
syncytial
virus,
metapneumovirus,
parainfluenza
rhinovirus,
seasonal
coronaviruses
stool,
urine,
mucus,
sputum,
saliva.
We
identified
220
sets
50
articles
reported
these
excretions.
Data
were
unevenly
distributed
across
virus
type
(with
most
available
for
influenza)
excretion
excretions).
Most
only
absence
cross-sectional
study
design.
There
is
need
more
concentration
data,
including
longitudinal
all
types.
Such
would
allow
quantitatively
linking
numbers
infected
individuals.
Environmental Science & Technology Letters,
Journal Year:
2023,
Volume and Issue:
11(1), P. 9 - 15
Published: Dec. 11, 2023
Climate
change
and
urbanization
are
increasing
the
distribution
of
insect
vectors
infectious
diseases.
Dengue
virus
is
an
arbovirus
that
causes
nearly
100
million
symptomatic
infections
per
year
endemic
in
124
countries,
range
its
mosquito
continues
to
increase.
Surveillance
dengue
complicated
by
fact
can
be
asymptomatic,
symptoms
may
not
readily
recognizable
clinicians.
Here
we
show
wastewater
monitoring
used
detect
RNA
yield
information
about
circulation
a
community.
We
collected
three
samples
solids
week
from
different
treatment
plants
Miami-Dade
County,
Florida,
where
were
locally
acquired.
Using
molecular
methods,
tested
for
4
serotypes
consistently
detected
3
at
all
did
other
serotypes.
According
publicly
available
data
on
infections,
vast
majority
caused
serotype
3.
Wastewater
detection
possible
with
as
few
4.23
laboratory
confirmed
cases
1
people
based
infection
data.
mSphere,
Journal Year:
2024,
Volume and Issue:
9(3)
Published: Feb. 27, 2024
Enteric
infections
are
important
causes
of
morbidity
and
mortality,
yet
clinical
surveillance
is
limited.
Wastewater-based
epidemiology
(WBE)
has
been
used
to
study
community
circulation
individual
enteric
viruses
panels
respiratory
diseases,
but
there
limited
work
studying
the
concurrent
a
suite
viruses.
A
retrospective
WBE
was
carried
out
at
two
wastewater
treatment
plants
located
in
California,
United
States.
Using
digital
droplet
polymerase
chain
reaction
(PCR),
we
measured
concentrations
human
adenovirus
group
F,
enteroviruses,
norovirus
genogroups
I
II,
rotavirus
nucleic
acids
solids
times
per
week
for
26
months
(
Water Research,
Journal Year:
2024,
Volume and Issue:
261, P. 122024 - 122024
Published: June 30, 2024
Water
quality,
critical
for
human
survival
and
well-being,
necessitates
rigorous
control
to
mitigate
contamination
risks,
particularly
from
pathogens
amid
expanding
urbanization.
Consequently,
the
necessity
maintain
microbiological
safety
of
water
supplies
demands
effective
surveillance
strategies,
reliant
on
collection
representative
samples
precise
measurement
contaminants.
This
review
critically
examines
advancements
passive
sampling
techniques
monitoring
in
various
systems,
including
wastewater,
freshwater,
seawater.
We
explore
evolution
conventional
materials
innovative
adsorbents
pathogen
capture
shift
culture-based
molecular
detection
methods,
underscoring
adaptation
this
field
global
health
challenges.
The
comparison
highlights
sampling's
efficacy
over
like
grab
its
potential
overcome
existing
challenges
through
use
such
as
granular
activated
carbon,
thermoplastics,
polymer
membranes.
By
evaluating
literature,
work
identifies
standardization
gaps
proposes
future
research
directions
augment
efficiency,
specificity,
utility
environmental
public
surveillance.
medRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Abstract
In
2024,
the
highly
pathogenic
avian
influenza
A
H5N1
caused
outbreaks
in
wild
birds,
poultry,
cows,
and
other
mammals
United
States
with
61
human
cases
also
reported
by
CDC.
Detection
of
H5
RNA
wastewater
has
been
previously
sewersheds
Texas
North
Carolina
nearby
impacted
dairy
herds
following
emergence
cows.
Here,
we
conduct
retrospective
testing
total
hemagglutinin
genes
as
well
presenting
applying
new
assays
for
detection
H1
H3
across
a
respiratory
virus
season
an
urban
California
sewershed
from
September
2023
–
May
2024.
Total
A,
H1,
were
regularly
detected,
while
was
first
detected
March.
We
developed
model
that
uses
Monte
Carlo
simulations
published
parameters
to
estimate
numbers
infected
people,
or
liters
H5-contaminated
milk
required
result
measured
concentrations
wastewater.
Our
findings
demonstrate
this
sewershed,
contaminated
poultry
most
likely
sources
created
publicly
available
tool
apply
input
inputs.
Synopsis
understand
potential
wastewater,
enabling
interpretation
detections.
TOC
figure