Exposure to 6-PPD Quinone at Environmentally Relevant Concentrations Causes Abnormal Locomotion Behaviors and Neurodegeneration in Caenorhabditis elegans

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(12), P. 4940 - 4950

Published: March 13, 2023

6-PPD quinone (6-PPDQ) can be transformed from through ozonation. Nevertheless, the potential neurotoxicity of 6-PPDQ after long-term exposure and underlying mechanism are largely unclear. In Caenorhabditis elegans, we here observed that 0.1–10 μg/L caused several forms abnormal locomotion behaviors. Meanwhile, neurodegeneration D-type motor neurons was in 10 6-PPDQ-exposed nematodes. The associated with activation Ca2+ channel DEG-3-mediated signaling cascade. this cascade, expressions deg-3, unc-68, itr-1, crt-1, clp-1, tra-3 were increased by 6-PPDQ. Moreover, among genes encoding neuronal signals required for control stress response, jnk-1 dbl-1 decreased 6-PPDQ, daf-7 glb-10 RNAi jnk-1, dbl-1, daf-7, resulted susceptibility to toxicity decreasing locomotory ability inducing neurodegeneration, suggesting JNK-1, DBL-1, DAF-7, GLB-10 also induction neurotoxicity. Molecular docking analysis further demonstrated binding DEG-3, GLB-10. Together, our data suggested risk at environmentally relevant concentrations causing organisms.

Language: Английский

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Screening p-Phenylenediamine Antioxidants, Their Transformation Products, and Industrial Chemical Additives in Crumb Rubber and Elastomeric Consumer Products

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(7), P. 2779 - 2791

Published: Feb. 9, 2023

Recently, roadway releases of

Language: Английский

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Four-week repeated exposure to tire-derived 6-PPD quinone causes multiple organ injury in male BALB/c mice

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 894, P. 164842 - 164842

Published: June 17, 2023

Language: Английский

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Environmental Occurrence and Toxicity of 6PPD Quinone, an Emerging Tire Rubber-Derived Chemical: A Review

Environmental Science & Technology Letters, Journal Year: 2023, Volume and Issue: 10(10), P. 815 - 823

Published: Sept. 1, 2023

N-(1,3-Dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD) is a chemical added to tires prevent their oxidative degradation. 6PPD highly reactive with ozone and oxygen, leading the formation of transformation products such as quinone (6PPDQ) on tire surfaces and, subsequently, in road wear particles. 6PPDQ toxicant that has been found roadway runoff receiving water systems. Its presence municipal stormwater led acute mortality coho salmon during migration urban creeks reproduce, generating global interest studying its occurrence toxicity environment. This review aims provide critical overview current state knowledge 6PPDQ, assisting researchers policymakers understanding potential impacts this emerging environment human health. As there are many unanswered questions surrounding further research needed. highlights importance including regulations for 6PPD, well all synthetic chemicals concern.

Language: Английский

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Urine Excretion, Organ Distribution, and Placental Transfer of 6PPD and 6PPD-Quinone in Mice and Potential Developmental Toxicity through Nuclear Receptor Pathways

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(36), P. 13429 - 13438

Published: Aug. 29, 2023

The rubber antioxidant 6PPD has gained significant attention due to its highly toxic transformation product, 6PPD-quinone (6PPDQ). Despite their detection in urines of pregnant women, the placental transfer and developmental toxicity 6PPDQ are unknown. Here, we treated C57Bl/6 mice with 4 mg/kg or investigate urine excretion transfer. Female male exhibited sex difference profiles 6PPDQ. Urine concentrations were one order magnitude lower than those 6PPD, suggesting higher bioaccumulation In from embryonic day 11.5 15.5, showed ∼1.5–8 times placenta, embryo body, brain, Using vitro dual-luciferase reporter assays, revealed that activated human retinoic acid receptor α (RARα) retinoid X (RXRα) at as low 0.3 μM, which was ∼10-fold detected urines. RXRα 1.2 μM. These results demonstrate exposure risks during pregnancy emphasize need for further toxicological epidemiological investigations.

Language: Английский

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Where the rubber meets the road: Emerging environmental impacts of tire wear particles and their chemical cocktails

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 927, P. 171153 - 171153

Published: March 7, 2024

About 3 billion new tires are produced each year and about 800 million become waste annually. Global dependence upon from natural rubber petroleum-based compounds represents a persistent complex environmental problem with only partial often-times, ineffective solutions. Tire emissions may be in the form of whole tires, tire particles, chemical compounds, which is transported through various atmospheric, terrestrial, aquatic routes built environments. Production use generates multiple heavy metals, plastics, PAH's, other that can toxic alone or as cocktails. Used require storage space, energy intensive to recycle, generally have few post-wear uses not also potential sources pollutants (e.g., crumb rubber, pavements, burning). particles emitted during major component microplastics urban runoff source unique highly potent substances. Thus, represent ubiquitous pollutant requires comprehensive examination develop effective management remediation. We approach issue pollution holistically by examining life cycle across production, emissions, recycling, disposal. In this paper, we synthesize recent research data human health risks associated use, disposal discuss gaps our knowledge fate transport, well toxicology leachates. examine remediation approaches for addressing exposure tires. consider three levels: their state, particulates, mixture Finally, information understanding outline key questions improve ability manage remediate pollution.

Language: Английский

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First insights into 6PPD-quinone formation from 6PPD photodegradation in water environment

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 459, P. 132127 - 132127

Published: July 23, 2023

Language: Английский

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Occurrence and Fate of Substituted p-Phenylenediamine-Derived Quinones in Hong Kong Wastewater Treatment Plants

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(41), P. 15635 - 15643

Published: Oct. 5, 2023

-Phenylenediamine quinones (PPD-Qs) are a newly discovered class of transformation products derived from

Language: Английский

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A new toxicity mechanism of N-(1,3-Dimethylbutyl)-N′-phenyl-p-phenylenediamine quinone: Formation of DNA adducts in mammalian cells and aqueous organisms

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 866, P. 161373 - 161373

Published: Jan. 5, 2023

Language: Английский

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Identification and quantification of tire wear particles by employing different cross-validation techniques: FTIR-ATR Micro-FTIR, Pyr-GC/MS, and SEM

Environmental Pollution, Journal Year: 2023, Volume and Issue: 326, P. 121511 - 121511

Published: March 24, 2023

Tire wear particles (TWPs) are one of the environment's most important emission sources microplastics. In this work, chemical identification these was carried out in highway stormwater runoff through cross-validation techniques for first time. Optimization a pre-treatment method (i.e., extraction and purification) provided to extract TWPs, avoiding their degradation denaturation, prevent getting low recognizable consequently underestimates quantification. Specific markers were used TWPs comparing real samples reference materials via FTIR-ATR, Micro-FTIR, Pyrolysis-gas-chromatography-mass spectrometry (Pyr-GC/MS). Quantification Micro-FTIR (microscopic counting); abundance ranged from 220,371 ± 651 TWPs/L 358,915 831 TWPs/L, while higher mass 39,6 9 mg lowest 31,0 8 TWPs/L. Most analyzed less than 100 μm size. The sizes also confirmed using scanning electron microscope (SEM), including presence potential nano samples. Elemental analysis SEM supported that complex mixture heterogeneous composition characterizes by agglomerating organic inorganic could derive brake road wear, pavement, dust, asphalts, construction work. Due analytical lack knowledge about quantification scientific literature, study significantly contributes providing novel methodology emerging contaminants runoff. results highlight uttermost necessity employ techniques, i.e., Pyr-GC/MS, environmental

Language: Английский

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