Fungicides
have
been
increasingly
used
across
various
sectors,
including
agriculture
and
textiles.
The
biocidal
properties
of
fungicides
may
negatively
impact
the
stability
intestinal
microbiota,
thereby
posing
a
threat
to
health.
In
this
study,
we
investigated
mechanisms
damage
functional
abnormalities
in
grass
carp
following
42-day
exposure
widely
fungicide
carbendazim
at
environmentally
relevant
concentrations
(0.2
20
μg/L).
Histopathological
observations,
mRNA
protein
expression
analyses,
biochemical
analysis,
quantification
short-chain
fatty
acids
(SCFAs),
cytokines,
lipopolysaccharide
(LPS),
16S
ribosomal
ribonucleic
acid
(rRNA),
as
well
internal
transcribed
spacer
(ITS)
sequencing,
were
performed.
At
concentrations,
strongly
induced
inflammation,
leading
increased
transcriptional
translational
levels
genes
involved
toll-like
receptor
five
(TLR5)/nuclear
factor
kappa
B
(NF-κB)
pathway,
TLR5,
NF-κB,
interleukin-1
beta
(IL-1β),
tumor
necrosis
factor-alpha
(TNFα).
Additionally,
damaged
barriers
reduced
tight
junction
proteins
(e.g.,
occludin
zonula
occludens-1/2),
goblet
cells,
immunoglobulin
M
levels,
while
also
disrupting
gut
microbiome,
metabolic
disorders,
particularly
decreases
SCFAs
increases
LPS.
Treatment
with
TLR5
antagonist
TH1020
mitigated
inflammation
caused
by
carbendazim,
subsequently
improving
mechanical
barrier
function.
Overall,
our
findings
provide
new
insights
into
toxicological
underlying
carp,
indicating
that
poses
significant
nontarget
organisms.
Given
its
widespread
detection
environment,
these
results
underscore
substantial
ecological
risks
health
fish
living
carbendazim-contaminated
water
bodies.
Ecotoxicology and Environmental Safety,
Journal Year:
2024,
Volume and Issue:
277, P. 116326 - 116326
Published: April 18, 2024
The
available
information
regarding
the
impact
of
antimony
(Sb),
a
novel
environmental
pollutant,
on
intestinal
microbiota
and
host
health
is
limited.
In
this
study,
we
conducted
physiological
characterizations
to
investigate
response
adult
zebrafish
different
concentrations
(0,
30,
300,
3000
µg/L)
Sb
over
period
14
days.
Biochemical
pathological
changes
demonstrated
that
effectively
compromised
integrity
physical
barrier
induced
inflammatory
responses
as
well
oxidative
stress.
Analysis
both
microbial
community
metabolome
revealed
exposure
0
30
µg/L
resulted
in
similar
structures;
however,
300
altered
communities'
composition
(e.g.,
decline
genus
Cetobacterium
an
increase
Vibrio).
Furthermore,
significantly
decreased
levels
bile
acids
glycerophospholipids
while
triggering
inflammation
but
activating
self-protective
mechanisms
such
antibiotic
presence.
Notably,
even
can
trigger
dysbiosis
metabolites,
potentially
impacting
fish
through
"microbiota-intestine-brain
axis"
contributing
disease
initiation.
This
study
provides
valuable
insights
into
toxicity-related
concerning
impacts
aquatic
organisms
with
significant
implications
for
developing
management
strategies.
Environmental Science & Technology,
Journal Year:
2024,
Volume and Issue:
58(11), P. 4937 - 4947
Published: March 6, 2024
Bis(2-ethylhexyl)-tetrabromophthalate
(TBPH),
a
typical
novel
brominated
flame
retardant,
has
been
ubiquitously
identified
in
various
environmental
and
biotic
media.
Consequently,
there
is
an
urgent
need
for
precise
risk
assessment
based
on
comprehensive
understanding
of
internal
exposure
the
corresponding
toxic
effects
specific
tissues.
In
this
study,
we
first
investigated
toxicokinetic
characteristics
TBPH
different
tissues
using
classical
pseudo-first-order
model.
We
found
that
was
prone
to
accumulate
liver
rather
than
gonad,
brain,
muscle
both
female
male
zebrafish,
highlighting
higher
liver.
Furthermore,
long-term
at
environmentally
relevant
concentrations
led
increased
visceral
fat
accumulation,
signaling
potential
abnormal
function.
Hepatic
transcriptome
analysis
predominantly
implicated
glycolipid
metabolism
pathways.
However,
alterations
profile
associated
genes
biochemical
indicators
revealed
gender-specific
responses
following
exposure.
Besides,
histopathological
observations
as
well
inflammatory
response
confirmed
development
nonalcoholic
fatty
disease,
particularly
zebrafish.
Altogether,
our
findings
highlight
liver,
enhancing
metabolic-disrupting
with
Ecotoxicology and Environmental Safety,
Journal Year:
2023,
Volume and Issue:
260, P. 115081 - 115081
Published: May 30, 2023
Niclosamide
(NIC)
is
the
only
commercially
available
molluscicide
for
controlling
schistosomiasis,
and
its
negative
effects
on
aquatic
animals
had
been
frequently
reported
in
recent
years.
However,
toxicity
mechanism
of
NIC
Chinese
soft-shelled
turtle
(Pelodiscus
sinensis)
have
not
yet
investigated.
Therefore,
juvenile
turtles
were
exposed
to
0
(control
group),
10
(low
NIC,
L),
50
(high
H)
μg/L
120
h
our
results
demonstrated
that
exposure
induced
severe
pathological
changes
liver
P.
sinensis.
And
typical
symptom
included
edema,
nuclear
migration
deformation,
vacuolization.
Compared
with
liver,
did
cause
significant
damage
gut
tissue.
In
addition,
DHE
staining
ROS
production
increased
increase
concentration
NIC.
The
activities
antioxidant
enzymes
including
superoxide
dismutase
(SOD),
glutathione
peroxidase
(GPx),
catalase
(CAT)
was
inhibited
malondialdehyde
(MDA)
content,
indicating
defense
significantly
perturbed.
Further,
transcriptome
sequencing
applied
evaluate
underlying
mechanisms
Pathway
enrichment
showed
disorder
lipid
metabolism
innate
immune
regulation,
Toll-like
receptors
(TLRs),
tumor
necrosis
factor
(TNF),
lectins,
complement
coagulation
cascades,
toxicological
properties
Overall,
current
study
provides
valuable
information
understand
toxic
effect
turtle.
