Published: Jan. 1, 2024
Language: Английский
Published: Jan. 1, 2024
Language: Английский
Journal of Aerosol Science, Journal Year: 2025, Volume and Issue: 186, P. 106555 - 106555
Published: Feb. 25, 2025
Language: Английский
Citations
0Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15
Published: Oct. 16, 2024
We describe a novel biosafety aerosol chamber equipped with state-of-the-art instrumentation for bubble-bursting generation, size distribution measurement, and condensation-growth collection to minimize sampling artifacts when measuring virus infectivity in particles. Using this facility, we investigated the effect of relative humidity (RH) very clean air without trace gases (except ∼400 ppm CO 2 ) on preservation influenza A (IAV) saline characterized terms 99%-inactivation time, t 99 , metric consider most relevant airborne transmission. The viruses remained infectious long namely > 5 h, if RH < 30% particles effloresced. Under intermediate conditions (40% 70%), loss was rapid ( ≈ 15–20 min, up 35 min at 95% RH). This is more than an order magnitude faster suggested by many previous studies aerosol-borne IAV, possibly due use matrices containing organic molecules, such as proteins, protective effects virus. tested hypothesis adding sucrose our aerosolization medium and, indeed, observed protection IAV (55%). Interestingly, measurements are also systematically lower those 1-μL droplet organic-free solutions, which cannot be explained particle alone.
Language: Английский
Citations
2bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: May 28, 2024
Abstract We describe a novel biosafety aerosol chamber equipped with state-of-the-art instrumentation for bubble-bursting generation, size distribution measurement, and condensation-growth collection to minimize sampling artifacts when measuring virus infectivity in particles. Using this facility, we investigated the effect of relative humidity (RH) very clean air without trace gases (except ∼400 ppm CO 2 ) on preservation influenza A (IAV) saline characterized terms 99%-inactivation time, t 99 , metric consider most relevant airborne transmission. The viruses remained infectious long namely > 5 h, if RH < 30% particles effloresced. Under intermediate conditions (40% 70%), loss was rapid ( ≈ 15-20 min, up 35 min at 95% RH). This is more than an order magnitude faster suggested by many previous studies aerosol-borne IAV, possibly due use matrices containing organic molecules, such as proteins, protective effects virus. tested hypothesis adding sucrose our aerosolization medium and, indeed, observed protection IAV (55 %). Interestingly, measurements are also systematically lower those 1-μL droplet organic-free solutions, which cannot be explained particle alone.
Language: Английский
Citations
1Building and Environment, Journal Year: 2024, Volume and Issue: 269, P. 112451 - 112451
Published: Dec. 13, 2024
Language: Английский
Citations
1Published: Jan. 1, 2024
Language: Английский
Citations
0