Polymer-Based Strategies for Enzyme Immobilization: A Comprehensive Review DOI Open Access
Gajanand Modi

Tuijin Jishu/Journal of Propulsion Technology, Journal Year: 2023, Volume and Issue: 44(5), P. 792 - 802

Published: Dec. 1, 2023

Enzyme immobilization refers to the process of attaching or confining enzymes onto a solid support within matrix, often made polymers other materials. This creates stable and controlled environment for enzyme interact with substrates perform catalysis. The primary goal is enhance stability, reusability, activity under specific conditions, making them more practical efficient various biotechnological, industrial, medical applications. Immobilization methods can vary widely, including physical adsorption, covalent bonding, entrapment matrices, encapsulation, crosslinking, more. These provide means control interactions between surrounding environment, affecting factors such as substrate accessibility, orientation, stability. Due their ease fabrication superior structural adaptability, polymer compounds in variety forms, beads, films, fibers,and coatings,have become popular supportive materials immobilization. For immobilization, number natural polymers, agar, agarose, alginate, dextran, chitosan,and carrageenan, well synthetic polyamides, polystyrene, polyacrylamide, are employed carrier system. offers cost-effective system applications biotechnology, industry, research.

Language: Английский

Biocatalytic Hydrogenation of Biomass-Derived Furan Aldehydes to Alcohols DOI
Zhigang Zhang, Xi Shen, Shi‐Kai Jiang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

The biomass-derived furan aldehydes furfural (FF) and 5-hydroxymethylfurfural (HMF) are versatile platform chemicals used to produce various value-added through further valorization processes. Selectively reducing C═O in FF HMF molecules form furfuryl alcohol (FAL) 2,5-bis(hydroxymethyl)furan (BHMF), represents an important research field upgrading biomass-based compounds. Currently, the reduction of alcohols chemical transformation often leads unavoidable environmental issues formation potential byproducts. Biocatalysis has demonstrated expanded applications converting into a diverse array chemicals. This process exhibits significant organic synthesis biotechnology due its green sustainable properties. biocatalytic especially route for selective FAL BHMF. review discusses recent progress biosynthesis BHMF from Recently discovered enzymes whole cells as biocatalysts production summarized. In addition, chemoenzymatic cascades synthesizing biomass hydrolysate raw materials also discussed.

Language: Английский

Citations

1

Integrating Enzymes with Supramolecular Polymers for Recyclable Photobiocatalytic Catalysis DOI Creative Commons
Jingping Ouyang, Zhenfang Zhang, Jian Li

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(16)

Published: Feb. 22, 2024

Chemical modifications of enzymes excel in the realm enzyme engineering due to its directness, robustness, and efficiency; however, challenges persist devising versatile effective strategies. In this study, we introduce a supramolecular modification methodology that amalgamates polymer with Candida antarctica lipase B (CalB) create (SupEnzyme). This approach features straightforward preparation amphiphilic (β-CD@SMA), which was subsequently conjugated enzyme, resulting SupEnzyme capable self-assembly into nanoparticles. The nanoparticles can form micron-scale aggregates through electrostatic interactions guest entities, thus enhancing catalyst recycling. Remarkably, these maintain 80 % activity after seven cycles, outperforming Novozym 435. Additionally, they effectively initiate photobiocatalytic cascade reactions using photocatalysts. As consequence, our exhibits noteworthy adaptability modification, presenting platform for various polymer, biocompatible pairings, potential applications fields chemistry biology.

Language: Английский

Citations

6

Conceptual Process Design and Techno-Economic Analysis of Biocatalytic Furfural Hydrogenation Using Ethanol as the Terminal Reductant DOI
Victor K. Sharma,

Thomas Binder,

Alan M. Allgeier

et al.

ACS Sustainable Chemistry & Engineering, Journal Year: 2024, Volume and Issue: 12(29), P. 10776 - 10785

Published: July 9, 2024

Redox enzyme-catalyzed reactions can be crucial to the development of a circular bio-economy by facilitating valorization biomass-based feedstock materials, such as bioethanol and furfural. The potential yeast alcohol dehydrogenase-catalyzed furfural reduction furfuryl using ethanol terminal reductant has been well-documented in literature free immobilized enzymes. In this study, systematic, comprehensive techno-economic analysis acetaldehyde coproduction is presented. A conceptual process design for production separation products developed, economics performed study challenges technology. Two scenarios derived from both actual experimental data hypothetical assumptions were studied understand factors behind economic feasibility. It was observed that high concentrations organics reactor product stream low cost starting raw materials are most feasibility achieving minimum selling prices coproducts could comparable current market prices. results form basis future life cycle analyses.

Language: Английский

Citations

2

Integrating Enzymes with Supramolecular Polymers for Recyclable Photobiocatalytic Catalysis DOI Creative Commons
Jingping Ouyang, Zhenfang Zhang, Jian Li

et al.

Angewandte Chemie, Journal Year: 2024, Volume and Issue: 136(16)

Published: Feb. 22, 2024

Abstract Chemical modifications of enzymes excel in the realm enzyme engineering due to its directness, robustness, and efficiency; however, challenges persist devising versatile effective strategies. In this study, we introduce a supramolecular modification methodology that amalgamates polymer with Candida antarctica lipase B (CalB) create (SupEnzyme). This approach features straightforward preparation amphiphilic (β‐CD@SMA), which was subsequently conjugated enzyme, resulting SupEnzyme capable self‐assembly into nanoparticles. The nanoparticles can form micron‐scale aggregates through electrostatic interactions guest entities, thus enhancing catalyst recycling. Remarkably, these maintain 80 % activity after seven cycles, outperforming Novozym 435. Additionally, they effectively initiate photobiocatalytic cascade reactions using photocatalysts. As consequence, our exhibits noteworthy adaptability modification, presenting platform for various polymer, biocompatible pairings, potential applications fields chemistry biology.

Language: Английский

Citations

0

Polymer-Based Strategies for Enzyme Immobilization: A Comprehensive Review DOI Open Access
Gajanand Modi

Tuijin Jishu/Journal of Propulsion Technology, Journal Year: 2023, Volume and Issue: 44(5), P. 792 - 802

Published: Dec. 1, 2023

Enzyme immobilization refers to the process of attaching or confining enzymes onto a solid support within matrix, often made polymers other materials. This creates stable and controlled environment for enzyme interact with substrates perform catalysis. The primary goal is enhance stability, reusability, activity under specific conditions, making them more practical efficient various biotechnological, industrial, medical applications. Immobilization methods can vary widely, including physical adsorption, covalent bonding, entrapment matrices, encapsulation, crosslinking, more. These provide means control interactions between surrounding environment, affecting factors such as substrate accessibility, orientation, stability. Due their ease fabrication superior structural adaptability, polymer compounds in variety forms, beads, films, fibers,and coatings,have become popular supportive materials immobilization. For immobilization, number natural polymers, agar, agarose, alginate, dextran, chitosan,and carrageenan, well synthetic polyamides, polystyrene, polyacrylamide, are employed carrier system. offers cost-effective system applications biotechnology, industry, research.

Language: Английский

Citations

1