Complex probiotics alleviate ampicillin-induced antibiotic-associated diarrhea in mice DOI Creative Commons
Wenwen Li, Siyu Zhang, Yanyan Wang

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: April 14, 2023

Antibiotic-associated diarrhea (AAD) is a common side effect during antibiotic treatment, which can cause dysbacteriosis of the gut microbiota. Previous studies have shown beneficial effects in AAD treatment with Bifidobacterium lactis XLTG11, Lactobacillus casei Zhang, plantarum CCFM8661, and rhamnosus Probio-M9. However, no been conducted on immunomodulatory protective intestinal barrier function four complex probiotics. The aim our study to investigate alleviation probiotics ampicillin-induced AAD.Thirty-six BALB/c mice were randomly divided into six groups: normal control group (NC), model (MC), low-, medium-, high-dose groups (LD, MD, HD), positive drug (Bifico, 1 × 107 cfu) (PDC; Bifico, also known as Triple Live Capsule, composed longum, acidophilus, Enterococcus faecalis). An was established by intragastric administration ampicillin, gavage different doses Bifico. weight gain, fecal water content, loose stool grade, permeability, total protein albumin levels, barrier, cytokine microbiota determined.The results showed that significantly decreased ileum tissue damage. Their application increased SIgA, TP, ALB levels. Additionally, levels pro-inflammatory cytokines those anti-inflammatory cytokines. Meanwhile, mRNA expression ZO-1, occludin, claudin-1, MUC2 upregulated probiotic-treated group. Furthermore, diversity modulated changes composition caused ampicillin. At phylum level, abundance Proteobacteria HD lower than MC group, whereas Bacteroidetes higher. genus abundances Klebsiella Parabacteroides lower, Bacteroides, Muribaculaceae, higher Moreover, Spearman's correlation analysis found several specific correlated AAD-related indicators.We improved diarrhea-related indexes, regulated diversity, barrier-related genes, preserved function, relieved inflammation injury, thereby effectively improving AAD-associated symptoms. Graphical Abstract.

Language: Английский

Protocatechuic Acid Alleviates Dextran-Sulfate-Sodium-Induced Ulcerative Colitis in Mice via the Regulation of Intestinal Flora and Ferroptosis DOI Creative Commons
Xuebin Yang, Xin Sun, Feng Zhou

et al.

Molecules, Journal Year: 2023, Volume and Issue: 28(9), P. 3775 - 3775

Published: April 27, 2023

Protocatechuic acid (PCA) is a natural component with multiple biological activities. However, the underlying mechanisms of effects PCA on anti-ulcerative colitis (UC) are unclear. A UC mouse model was established by allowing mice to freely drink dextran sulfate sodium solution. The were administered intragastrically for 7 days. Histological pathology, intestinal flora, and ferroptosis regulators determined in vivo. Additionally, ferroptotic Caco-2 cells modeled investigate role ferroptosis. Our results showed that reduced levels disease activity index, inflammatory factors, histological damage mice. We also found regulation especially Bacteroidetes, one potential protective anti-UC. Moreover, downregulated level colon tissue, as evidenced iron overload, decreased glutathione depletion, lower malondialdehyde production compared group. Similar observed Erastin-treated cells. obtained using reactive oxygen species assays changes mitochondrial structure via scanning electron microscopy support these results. findings suggested protected against regulating flora

Language: Английский

Citations

29
Phlorizin Mitigates Dextran Sulfate Sodium-Induced Colitis in Mice by Modulating Gut Microbiota and Inhibiting Ferroptosis DOI
Ji Cheng, Dianfeng Liu, Ya‐Ping Huang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(43), P. 16043 - 16056

Published: Oct. 19, 2023

Phlorizin (PHZ) is the main active component of apple peel and presents a potential application value. In past few years, some reports have suggested that PHZ may antioxidant anti-inflammatory effects. Herein, we attempted to assess protective effects on dextran sodium sulfate (DSS)-induced colitis in mice determine underlying molecular mechanisms. Our results early intervention with (20, 40, 80 mg/kg) significantly reduced severity DSS-induced mice, as presented by longer colon, improved tight junction protein, decreased disease activity index, attenuated inflammatory factors. Additionally, + inhibited ferroptosis decreasing surrogate marker levels (MDA Iron Content). (80 increased diversity intestinal flora colitic elevating beneficial bacteria (Lactobacillaceae Muribaculaceae) reducing harmful (Lachnospiraceae). This indirectly led an increase amount short-chain fatty acids. A fecal microbial transplantation (FMT) test was conducted show ameliorated ulcerative (UC) regulating gut dysbiosis. conclusion, preserving their barrier flora.

Language: Английский

Citations

23
Polysaccharides from Alpinia oxyphylla fruit prevent hyperuricemia by inhibiting uric acid synthesis, modulating intestinal flora and reducing renal inflammation DOI
Fei Ren, Jinji Lin,

Mengxu Zhu

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 278, P. 134782 - 134782

Published: Aug. 14, 2024

Language: Английский

Citations

10
Bifidobacterium dentium N8 with potential probiotic characteristics prevents LPS-induced intestinal barrier injury by alleviating the inflammatory response and regulating the tight junction in Caco-2 cell monolayers DOI
Li Zhao,

Qinggang Xie,

Smith Etareri Evivie

et al.

Food & Function, Journal Year: 2021, Volume and Issue: 12(16), P. 7171 - 7184

Published: Jan. 1, 2021

The intestinal barrier is vital for preventing inflammatory bowel disease (IBD). This study aimed to investigate the potential mechanism behind protective effects of B. dentium N8 on using lipopolysaccharide (LPS)-induced Caco-2 cells model. Our probiotic validation results showed that had a higher adhesion ability and more substantial inhibition effect Escherichia coli ATCC 25922 HT-29 cells. Regarding epithelial integrity, significantly increased trans-epithelial electrical resistance (TEER) value decreased paracellular permeability stimulated by (LPS). In addition, ZO-1, occludin, claudin-1 mRNA expression. downregulated expression level TLR4 pro-inflammatory cytokines (TNF-α, IL-1β, IL-6). Furthermore, better than E7. Comparative genomics E7 specific genes encoding immune system regulation. findings provide theoretical basis possessing barrier, which indicate it could be used as novel therapy IBD.

Language: Английский

Citations

52
Bifidobacterium longum Ameliorates Dextran Sulfate Sodium-Induced Colitis by Producing Conjugated Linoleic Acid, Protecting Intestinal Mechanical Barrier, Restoring Unbalanced Gut Microbiota, and Regulating the Toll-Like Receptor-4/Nuclear Factor-κB Signaling Pathway DOI
Chen Yang,

Haiqin Chen,

Jiuhong Ding

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2021, Volume and Issue: 69(48), P. 14593 - 14608

Published: Nov. 29, 2021

This study aimed to explore the effects and differences of conjugated linoleic acid (CLA)-producing Bifidobacterium longum on alleviation dextran sulfate sodium (DSS)-induced colitis its patterns. Different B. strains were administered at 109 cfu/day 7 days before DSS treatment. CCFM681 significantly increased goblet cells, mucin2 (MUC2), claudin-3, α-catenin1, ZO-1, but neither CCFM760 nor CCFM642 had those protective effects. Interleukin-6 (IL-6) tumor necrosis factor-alpha (TNF-α) downregulated, while IL-10 was upregulated by CCFM642. Moreover, treatment inhibited toll-like receptor-4 (TLR4)/nuclear factor kappa-B (NF-κB) pathway. Furthermore, rebalanced gut microbiota via regulating diversity key microorganisms. Colonic CLA concentrations in mice fed with higher than that DSS-exposed mice, groups showed insignificant difference compared group. a positive correlation effectiveness relieving colitis. alleviated protecting intestinal mechanical barrier, modulating microbiota, inhibiting TLR4/NF-κB pathway associated pro-inflammatory cytokines. These results will help clinical trials probiotics development functional products for

Language: Английский

Citations

51
Bifidobacterium breve Alleviates DSS-Induced Colitis in Mice by Maintaining the Mucosal and Epithelial Barriers and Modulating Gut Microbes DOI Open Access
Mengmeng Niu,

Huan-Xin Guo,

Jun-Wu Cai

et al.

Nutrients, Journal Year: 2022, Volume and Issue: 14(18), P. 3671 - 3671

Published: Sept. 6, 2022

This study was designed to explore the different intestinal barrier repair mechanisms of Bifidobacterium breve (B. breve) H4-2 and H9-3 with exopolysaccharide (EPS) production in mice colitis. The lipopolysaccharide (LPS)-induced IEC-6 cell inflammation model dextran sulphate sodium (DSS)-induced colitis were used. Histopathological changes, epithelial integrity, short-chain fatty acid (SCFA) content, cytokine levels, NF-κB expression level, flora analyzed evaluate role B. alleviating Cell experiments indicated that both strains could regulate levels. In vivo confirmed oral administration significantly increased mucin, occludin, claudin-1, ZO-1, decreased levels IL-6, TNF-α, IL-1β IL-10. Both also inhibited signaling pathway. Moreover, intervention SCFAs, reduced abundance Proteobacteria Bacteroidea, Muribaculaceae. These results demonstrate EPS-producing can physical, immune, microbial damage caused by DSS mice. Of two strains, had a higher EPS output more effective at than H9-3. will provide insights useful for clinical applications development probiotic products treatment

Language: Английский

Citations

37
Heat-Killed Bifidobacterium bifidum B1628 May Alleviate Dextran Sulfate Sodium-Induced Colitis in Mice, and the Anti-Inflammatory Effect Is Associated with Gut Microbiota Modulation DOI Open Access
Cuijiao Feng,

Weiqin Zhang,

Tao Zhang

et al.

Nutrients, Journal Year: 2022, Volume and Issue: 14(24), P. 5233 - 5233

Published: Dec. 8, 2022

Inflammatory bowel disease (IBD) is a chronic inflammatory associated with gut dysbiosis. This study aimed to investigate the effects of heat-killed Bifidobacterium bifidum B1628 (HB1628) in dextran sulfate sodium (DSS)-induced colitis mice. The following three mouse groups were included (n = eight per group): NC (normal control), DSS (colitis), and HB1628 (colitis postbiotic). mice group showed significant weight loss histological damage, developed bloody diarrhea, scored high activity index (DAI), exhibited increases pro-inflammatory cytokines (interleukin [IL]-1β, IL-6, tumor necrosis factor [TNF]-α) decreases an anti-inflammatory cytokine (IL-13) serum. These changes accompanied by microbiota modulation (decreases Rikenellaceae Eubacterium; Peptostreptococcaceae, Bacteroides vulgatus, Parasutterella excrementihominis). had lower DAIs, histology scores, serum levels (IL-1β TNF-α), but higher (IL-13), compared group, suggesting less severe state after intervention. Additionally, improved DSS-induced dysbiosis, which evidenced intestinal beneficial bacteria, such as Lactobacillus, known unfavorable taxa IBD, e.g., Porphyromonadaceae, Subdoligranulum, Lachnospiraceae bacterium 3_1_46FAA, Alistipes indistinctus. Functional metagenomics revealed significantly enriched metabolic pathways (namely, aerobic respiration I [cytochrome c] pathway superpathways L-phenylalanine biosynthesis L-tryptophan biosynthesis, respectively). In conclusion, our results that effectively inflammation tissue damage mice, symptom relief effect was obvious remodulation.

Language: Английский

Citations

36
Exopolysaccharide from Bifidobacterium breve alleviate dextran sulfate sodium-induced colitis in mice via inhibiting oxidative stress and regulating intestinal flora DOI
Mengmeng Niu,

Huan-Xin Guo,

Jun-Wu Cai

et al.

Food Bioscience, Journal Year: 2023, Volume and Issue: 56, P. 103020 - 103020

Published: Aug. 19, 2023

Language: Английский

Citations

20
Propionic Acid Driven by the Lactobacillus johnsonii Culture Supernatant Alleviates Colitis by Inhibiting M1 Macrophage Polarization by Modulating the MAPK Pathway in Mice DOI
Zhifeng Wu,

Jinhui He,

Zeyue Zhang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(41), P. 14951 - 14966

Published: Oct. 3, 2023

In this study, we investigated the effects of Lactobacillus johnsonii on mouse colitis model. The results showed that supernatant L. culture alleviated and remodeled gut microbiota, represented by an increased abundance bacteria producing short-chain fatty acids, leading to concentration propionic acid in intestine. Further studies revealed inhibited activation MAPK signaling pathway polarization M1 macrophages. Macrophage clearance assays confirmed macrophages are indispensable for alleviating through acid. vitro experiments directly reduced macrophage polarization, thereby inhibiting secretion pro-inflammatory cytokines. These findings improve our understanding how attenuates inflammatory bowel disease (IBD) provide valuable insights identifying molecular targets IBD treatment future.

Language: Английский

Citations

20
Apigenin-7-O-glucoside alleviates DSS-induced colitis by improving intestinal barrier function and modulating gut microbiota DOI Creative Commons

Yeye Hu,

Xueting Guan,

Ziliang He

et al.

Journal of Functional Foods, Journal Year: 2023, Volume and Issue: 104, P. 105499 - 105499

Published: March 21, 2023

Apigenin-7-O-glucoside (AG), a flavonoid glycoside, has been shown in vitro anti-inflammatory activities. To date, the mechanism of AG against colitis not reported. In this study, we investigated role relieving and explored its potential mechanisms dextran sulfate sodium (DSS)-induced mice. The results indicated oral administration increased colon length improved colonic histopathology. Besides, it dramatically restored expression pro-inflammatory mediators, as well enhanced intestinal barrier markers such ZO-1, occludin, claudin-1, claudin-3. mitogen-activated protein kinases (MAPKs) including ERK, JNK, p38 were significantly inhibited. Moreover, 16S rDNA sequencing revealed that abundance Akkermansia, reduced Bacteroides Desulfovibrio. Taken together, supplementation relieved DSS-induced by protecting barrier, inhibiting MAPK pathway, modulating gut microbiota, which may be good candidate therapeutic compound for sustaining health to lower risk colitis.

Language: Английский

Citations

18