Structural Characterization and Immunomodulatory Activity of a Mannose-Rich Polysaccharide Isolated from Bifidobacterium breve H4–2

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(49), P. 19791 - 19803

Published: Nov. 30, 2023

In this study, a novel homogeneous mannose-rich polysaccharide named EPS-1 from the fermentation broth of Bifidobacterium breve H4–2 was isolated and purified by anion exchange column chromatography gel chromatography. The primary structure analyzed high-performance liquid chromatography, Fourier-transform infrared spectroscopy, gas chromatography–mass spectrometry, nuclear magnetic resonance. results indicated that had typical functional groups polysaccharides. with an average molecular weight 3.99 × 104 Da mainly composed mannose (89.65%) glucose (5.84%). backbone →2,6)-α-d-Manp-(1→2)-α-d-Manp-(1→2,6)-α-d-Manp-(1→2)-α-d-Manp-(1→2,6)-α-d-Manp-(1→6)-α-d-Glcp-(1→ simultaneously containing two kinds branched chains (α-d-Manp-(1→3)-α-d-Manp-(1→ α-d-Manp-(1→). Besides, triple-helical conformation exhibited excellent thermal stability. Moreover, immunomodulatory activity evaluated RAW 264.7 cells. Results significantly enhanced viability could also be recognized toll-like receptor 4, thereby activating factors-κB (NF-κB) signaling pathway, promoting phosphorylation related transcription factors, improving cell phagocytic activity, secretion NO, IL-6, IL-1β, TNF-α. Thus, activate TLR4-NF-κB pathway to emerge on macrophages. above indicate can serve as potential immune-stimulating polysaccharide.

Language: Английский

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Protective Effects of Bifidobacterium Breve MCC1274 as a Novel Therapy for Alzheimer’s Disease

Nutrients, Journal Year: 2025, Volume and Issue: 17(3), P. 558 - 558

Published: Jan. 31, 2025

Alzheimer’s disease (AD) is the most common form of dementia and characterized by memory impairment that significantly interferes with daily life. Therapeutic options for AD substantively modify progression remain a critical unmet need. In this regard, gut microbiota crucial in maintaining human health regulating metabolism immune responses, increasing evidence suggests probiotics, particularly beneficial bacteria, can enhance cognitive functions. Recent studies have highlighted positive effects Bifidobacterium breve MCC1274 (B. MCC1274) on individuals mild (MCI) schizophrenia. Additionally, oral supplementation B. has been shown to effectively prevent decline AppNL–G–F mice. relation pathology, found reduce amyloid-β (Aβ) accumulation tau phosphorylation both wild-type (WT) It also decreases microglial activation increases levels synaptic proteins. review, we examine AD, exploring potential mechanisms action how probiotic strain may aid preventing or treating disease. Furthermore, discuss broader implications improving overall host provide insights into future research directions promising therapy.

Language: Английский

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Faecalibacterium prausnitzii, Bacteroides faecis and Roseburia intestinalis attenuate clinical symptoms of experimental colitis by regulating Treg/Th17 cell balance and intestinal barrier integrity

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 167, P. 115568 - 115568

Published: Oct. 2, 2023

Ulcerative colitis (UC) is a severe inflammatory bowel disease (IBD) characterized by multifactorial complex disorders triggered environmental factors, genetic susceptibility, and also gut microbial dysbiosis. Faecalibacterium prausnitzii, Bacteroides faecis, Roseburia intestinalis are underrepresented species in UC patients, leading to the hypothesis that therapeutic application of those bacteria could ameliorate clinical symptoms severity. Acute was induced mice 3.5% DSS, commensal bacterial were administered oral gavage simultaneously with DSS treatment for up 7 days. The signs colonic inflammation, intestinal barrier integrity, proportion regulatory T cells (Tregs), expression pro-inflammatory anti-inflammatory cytokines quantified. concentrations SCFAs feces measured using Gas-liquid chromatography. microbiome analyzed all groups at endpoint experiment. Results benchmarked against contemporary mesalazine regime. We show alone combination reduced activity index scores, inhibited colon shortening, strengthened epithelial barrier, positively modulated tight junction protein expression. level significantly reduced. Immune modulation occurred via inhibition loss CD4 +CD25 +Treg spleen. Our study proofed F. B. R. ameliorated DSS-induced symptoms, histological immune status. data suggest these positive effects mediated immune-modulatory pathways influence on Treg/Th17 balance.

Language: Английский

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The role of intestinal microbes on intestinal barrier function and host immunity from a metabolite perspective

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 9, 2023

The gut is colonized by many commensal microorganisms, and the diversity metabolic patterns of microorganisms profoundly influence intestinal health. These microbial imbalances can lead to disorders such as inflammatory bowel disease (IBD). Microorganisms produce byproducts that act signaling molecules, triggering immune system in mucosa controlling inflammation. For example, metabolites like short-chain fatty acids (SCFA) secondary bile release inflammatory-mediated signals binding specific receptors. indirectly affect host health immunity interacting with epithelial mucosal cells. Moreover, Tryptophan-derived also play a role governing response aromatic hydrocarbon receptors (AHR) located on mucosa, enhancing barrier. Dietary-derived indoles, which are synthetic precursors AHR ligands, work together SCFA reduce stress epithelium regulate This review highlights interaction between system, well crosstalk dietary fiber intake improving metabolism its beneficial effects organism.

Language: Английский

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Oral delivery of porous starch-loaded bilayer microgels for controlled drug delivery and treatment of ulcerative colitis

Carbohydrate Polymers, Journal Year: 2023, Volume and Issue: 314, P. 120887 - 120887

Published: April 11, 2023

Language: Английский

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Enteric-coated cerium dioxide nanoparticles for effective inflammatory bowel disease treatment by regulating the redox balance and gut microbiome

Biomaterials, Journal Year: 2024, Volume and Issue: 314, P. 122822 - 122822

Published: Sept. 12, 2024

Language: Английский

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Probiotics for the treatment of ulcerative colitis: a review of experimental research from 2018 to 2022

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: July 6, 2023

Ulcerative colitis (UC) has become a worldwide public health problem, and the prevalence of disease among children been increasing. The pathogenesis UC not elucidated, but dysbiosis gut microbiota is considered main cause chronic intestinal inflammation. This review focuses on therapeutic effects probiotics potential mechanisms involved. In animal studies, have shown to alleviate symptoms UC, including weight loss, diarrhea, blood in stool, shortened colon length, while also restoring microecological homeostasis, improving barrier function, modulating immune response, attenuating inflammation, thereby providing theoretical support for development probiotic-based microbial products as an adjunctive therapy UC. However, efficacy influenced by factors such bacterial strain, dose, form. Hence, action need be investigated further. Relevant clinical trials are currently lacking, so extension experimental findings application requires longer period consideration validation.

Language: Английский

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Exopolysaccharide from Bifidobacterium breve alleviate dextran sulfate sodium-induced colitis in mice via inhibiting oxidative stress and regulating intestinal flora

Food Bioscience, Journal Year: 2023, Volume and Issue: 56, P. 103020 - 103020

Published: Aug. 19, 2023

Language: Английский

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Lactiplantibacillus plantarum HY7718 Improves Intestinal Integrity in a DSS-Induced Ulcerative Colitis Mouse Model by Suppressing Inflammation through Modulation of the Gut Microbiota

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(1), P. 575 - 575

Published: Jan. 1, 2024

Inflammatory bowel disease (IBD), a chronic condition that causes persistent inflammation in the digestive system, is closely associated with intestinal microbiome. Here, we evaluated effects of Lactiplantibacillus plantarum HY7718 (HY7718) on IBD symptoms mice dextran sulfate sodium (DSS)-induced colitis. Oral administration led to significant improvement activity index score and histological index, as well preventing weight loss, model mice. upregulated expression tight junction (TJ)-related genes downregulated encoding pro-inflammatory cytokines involved TLR/MyD88/NF-κB signaling pathway. Additionally, reduced blood levels cytokines, reversing DSS-induced changes composition also increased percentage beneficial bacteria (Lactiplantibacillus Bifidobacterium), which correlated positively TJ-related genes. Finally, decreased population pathogens such Escherichia, symptoms. The data suggest improves integrity colitis by regulating TJ proteins inflammatory microflora. Thus, L. may be suitable functional supplement gut health.

Language: Английский

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Bifidobacterium and the intestinal mucus layer

Microbiome Research Reports, Journal Year: 2023, Volume and Issue: 2(4)

Published: Sept. 27, 2023

Bifidobacterium species are integral members of the human gut microbiota and these microbes have significant interactions with intestinal mucus layer. This review delves into -mucus dynamics, shedding light on multifaceted nature this relationship. We cover conserved features interactions, such as adhesion positive regulation goblet cell production, well strain-specific attributes degradation. For each interface, we explore molecular mechanisms underlying their potential implications for health. Notably, emphasize ability to positively influence layer, its a mucin-builder therapeutic agent diseases associated disrupted barriers. By elucidating complex interplay between mucus, aim contribute deeper understanding microbiota-host interface pave way novel strategies.

Language: Английский

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