Multivalent fucosides targeting β-propeller lectins DOI Open Access
Margherita Duca

Published: March 1, 2023

Fungal and bacterial pathogens causing lung infections often use lectins to mediate adhesion glycoconjugates at the surface of host tissues. Given rapid emergence resistance treatments in current use, β-propeller such as FleA from Aspergillus fumigatus, SapL1 Scedosporium apiospermum, BambL Burkholderia ambifaria have become appealing targets for design anti-adhesive molecules. In search novel cheap anti-infectious agents, we synthesized multivalent compounds that can display up 20 units fucose, natural ligand. We obtained nanomolar inhibitors are several orders magnitude stronger than their monovalent analogue according biophysical techniques (i.e., fluorescence polarization, isothermal titration calorimetry, bio-layer interferometry). The reason high affinity might be attributed a strong aggregating mechanism, which was examined by analytical ultracentrifugation. Additional structural biology, X-ray crystallography atomic force microscopy, were used try decipher binding mode towards targets. Finally, fucosylated reduce A. fumigatus spores epithelial cells when administered 1 h before or after infection human cells. For this reason, propose them promising drugs prevention treatment aspergillosis related microbial infections.

Language: Английский

Modulation of tryptophan metabolism via AHR-IL22 pathway mediates the alleviation of DSS-induced colitis by chitooligosaccharides with different degrees of polymerization DOI
Yu Wang, Xiaoguo Ji, Mengyao Zhao

et al.

Carbohydrate Polymers, Journal Year: 2023, Volume and Issue: 319, P. 121180 - 121180

Published: July 12, 2023

Language: Английский

Citations

14
Utilization of glycosyltransferases as a seamless tool for synthesis and modification of the oligosaccharides-A review DOI
M. Ali, Fakhra Liaqat, Mahammed Ilyas Khazi

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 249, P. 125916 - 125916

Published: July 30, 2023

Language: Английский

Citations

13
Isolation of Human Milk Difucosyl Nona- and Decasaccharides by Ultrahigh-Temperature Preparative PGC-HPLC and Identification of Novel Difucosylated Heptaose and Octaose Backbones by Negative-Ion ESI-MSn DOI Creative Commons
Cuiyan Cao,

Yi‐Ming Cheng,

Yi Zheng

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(16), P. 6170 - 6179

Published: April 15, 2024

Despite their many important physiological functions, past work on the diverse sequences of human milk oligosaccharides (HMOs) has been focused mainly highly abundant HMOs with a relatively low degree polymerization (DP) due to lack efficient methods for separation/purification and high-sensitivity sequencing large-sized DP ≥ 10. Here we established an ultrahigh-temperature preparative HPLC based porous graphitized carbon column at up 145 °C overcome anomeric α/β splitting problem developed further negative-ion ESI-CID-MS/MS into multistage MSn using combined product-ion scanning singly charged molecular ion doubly fragment branching Gal adjacent GlcNAc residues. The separation method allows neutral fraction 10 70 components, among which 17 isomeric difucosylated nona- decasaccharides were purified sequenced. As result, novel branched difucosyl heptaose octaose backbones unambiguously identified in addition conventional linear backbones. structures DF-novo-heptaose, DF-novo-LNO I, DF-novo-LNnO I corroborated by NMR. various fucose-containing Lewis epitopes different confirmed oligosaccharide microarray analysis.

Language: Английский

Citations

4
Patterns of Human Milk Oligosaccharides in Mature Milk Are Associated with Certain Gut Microbiota in Infants DOI Open Access
Shuai Mao, Ai Zhao, Hua Jiang

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(9), P. 1287 - 1287

Published: April 25, 2024

Human milk oligosaccharides (HMOs) are complexes that play a crucial role in shaping the early-life gut microbiota. This study intends to explore whether HMO patterns associated with microbiota of infants. We included 96 Chinese breastfeeding mother–infant dyads. Breast and infant faecal samples were collected tested. With 2′-fucosyllactose, difucosyllactose, lacto-N-fucopentaose-I as biomarkers, we divided mothers into secretor non-secretor groups. extracted using principal component analysis. The majority (70.7%) categorised five different identified. After adjustment, infants exhibited lower relative abundance Bifidobacterium bifidum (β = −0.245, 95%CI: −0.465~−0.025). An pattern characterised by high levels 3-fucosyllactose, lacto-N-fucopentaose-III, lacto-N-neodifucohexaose-II was positively breve (p 0.014), while lacto-N-neotetraose, 6′-sialyllactose, sialyllacto-N-tetraose-b negatively 0.027). monofucosyl-lacto-N-hexaose-III monofucosyl-lacto-N-neohexaose dentium 0.025) < 0.001), respectively. suggests from mature breast certain breastfed

Language: Английский

Citations

4
Regioselective Enzymatic Galactosylation Enabled Divergent Synthesis of Asymmetrical Biantennary Human Milk Oligosaccharides DOI
Hui Xia,

Kan Zhong,

Yun Li

et al.

ACS Catalysis, Journal Year: 2024, Volume and Issue: 14(17), P. 13390 - 13399

Published: Aug. 23, 2024

Human milk oligosaccharides (HMOs) are essential nutritional and bioactive components of human breast milk, conferring numerous beneficial effects on neonatal health. The HMO repertoire comprises over 200 unique glycan structures, more than half them branched oligosaccharides. Owing to the structural diversity complexity, synthesis HMOs remains challenging, which significantly hinders their functional study. We herein present a highly efficient biomimetic approach for rapid scaled-up chemoenzymatic asymmetrical biantennary HMOs. Taking advantage inherent regioselectivity panel bacterial galactosyltransferases, two pentasaccharide intermediates were obtained through regioselective enzymatic β1,3- or β1,4-galactosylation readily available chemically synthesized symmetrical tetrasaccharide precursor. further diversified by sequential multienzyme cascade reactions afford library 20 structurally well-defined lacto-N-hexaose (LNH) lacto-N-neo-hexaose (LNnH) series in less 5 linear steps.

Language: Английский

Citations

4
3-Fucosyllactose Prevents Nonalcoholic Fatty Liver Disease by Modulating the Gut Microbiota-Derived Pantothenate in Mice DOI
Bei Liu, Ningning He, Hui Li

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Nonalcoholic fatty liver disease (NAFLD) is a growing global health threat. Human milk oligosaccharides (HMOs) exhibit prebiotic properties that may alleviate NAFLD progression. Herein, our study demonstrates 3-fucosyllactose (3-FL), distinctive and crucial HMO, significantly attenuates body weight gain, enhances hepatic lipid metabolism, reduces inflammation in high-fat diet (HFD)-induced mouse model. These findings suggest its potential as dietary supplement for preventing alleviating Subsequently, fecal metagenomic nontargeted metabolomics analyses revealed 3-FL treatment alleviated HFD-induced gut microbiota dysbiosis, with specific enhancement of the pantothenate (vitamin B5) metabolic pathways. Our targeted metabolite analysis further significant increase both concentrations, which contributed to coenzyme A (CoA)-mediated metabolism pathway. Furthermore, subsequent population cohort correlation between serum levels progression NAFLD, thereby reinforcing candidacy noninvasive diagnostic biomarker. show acts an effective symptoms, part by enhancing microbiota-mediated pantothenate/CoA

Language: Английский

Citations

0
Recent advances in targeted manipulation of the gut microbiome by prebiotics: from taxonomic composition to metabolic function DOI

Songtao Fan,

Zhihong Zhang, Yansheng Zhao

et al.

Current Opinion in Food Science, Journal Year: 2022, Volume and Issue: 49, P. 100959 - 100959

Published: Nov. 14, 2022

Language: Английский

Citations

18
Human Milk Oligosaccharides Ameliorate Intestinal Barrier in Aging Honey Bee by Regulating Gut Microbiota DOI
Xiaodong Lin, Yingying Lin, Yueyi Wang

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 105954 - 105954

Published: Jan. 1, 2025

Language: Английский

Citations

0
Comprehensive review on human Milk oligosaccharides: Biosynthesis, structure, intestinal health benefits, immune regulation, neuromodulation mechanisms, and applications DOI

Jieting Yu,

Le Li, Supaluck Kraithong

et al.

Food Research International, Journal Year: 2025, Volume and Issue: 209, P. 116328 - 116328

Published: April 6, 2025

Language: Английский

Citations

0
Viruses and Human Milk: Transmission or Protection? DOI Creative Commons
Rachele Francese, Chiara Peila, Manuela Donalisio

et al.

Advances in Nutrition, Journal Year: 2023, Volume and Issue: 14(6), P. 1389 - 1415

Published: Aug. 20, 2023

Human milk (HM) is considered the best source of nutrition for infant growth and health. This nourishment unique changes constantly during lactation to adapt physiological needs developing infant. It also recognized as a potential route transmission some viral pathogens although presence virus in HM rarely leads disease an intriguing paradox can be explained by considering intrinsic antiviral properties HM. In this comprehensive schematically presented review, we have described what viruses been detected so far their risk through breastfeeding is. We provided description all compounds HM, along with analysis demonstrated hypothesized mechanisms action. Finally, analyzed impact pasteurization storage methods on detection viruses, highlighted that there currently deep knowledge The current evidence suggests that, most cases, it unnecessarily deprive high-quality continuation interest mother.

Language: Английский

Citations

9