Alanine to glycine substitution in the PyR2 confers sodium channel resistance to Type I pyrethroids DOI Open Access
Mengli Chen, Likui Wang,

Xiangyi Zhou

et al.

Pest Management Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 30, 2024

Abstract BACKGROUND Aedes aegypti is a primary urban vector of dengue, yellow fever, Zika and chikungunya worldwide. Pyrethroid insecticides are the most effective for controlling Ae. . However, pyrethroid resistance has developed due to long‐term overuse insecticides, many knockdown (kdr) mutations have been identified in resistant populations. A1007G, an alanine glycine substitution, was found from Vietnam Malaysia, which always co‐existed with F1534C V1016G. role A1007G linkage or V1016G remain unknown. RESULTS In this study, we examined effects on sodium channel gating properties sensitivity Xenopus oocytes. We + shifted voltage dependence activation depolarizing direction. Mutations inactivation Both reduced two Type I pyrethroids, permethrin bifenthrin, synergistic were observed between F1534C. none mutations, affected II deltamethrin cypermethrin. Furthermore, triple significantly both pyrethroids. CONCLUSION had distinct effect I, but not also exhibited will provide fundamental insight into molecular interactions insect © 2024 Society Chemical Industry.

Language: Английский

Role of mutation G255A in modulating pyrethroid sensitivity in insect sodium channels DOI
Kaiyang Liu, Likui Wang,

S.X. Wen

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 295, P. 139455 - 139455

Published: Jan. 7, 2025

Language: Английский

Citations

1
A tiny sample rapid visual detection technology for imidacloprid resistance in Aphis gossypii by CRISPR/Cas12a DOI

Rujing Kang,

Ren Li,

Juma Mjengi

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 951, P. 175712 - 175712

Published: Aug. 23, 2024

Language: Английский

Citations

4
The hygienic insecticide dimefluthrin induced neurodevelopmental deficits and behavioral disorders in zebrafish (Danio rerio) larvae DOI
Ruirui Li, Wenping Xu,

Qiao‐Qiao Li

et al.

Environmental Research, Journal Year: 2025, Volume and Issue: 268, P. 120777 - 120777

Published: Jan. 6, 2025

Language: Английский

Citations

0
Population genetics as a tool to understand invasion dynamics and insecticide resistance in indoor urban pest insects DOI Creative Commons
Warren Booth

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 62, P. 101166 - 101166

Published: Jan. 21, 2024

Language: Английский

Citations

3
Cardiotoxicity risk induced by sanitary insecticide Dimefluthrin DOI
Ruirui Li, Ning Zhou, Cheng Zhang

et al.

Chemosphere, Journal Year: 2024, Volume and Issue: 364, P. 142910 - 142910

Published: July 26, 2024

Language: Английский

Citations

2
Molecular Detection of kdr and superkdr Mutation Sites and Analysis of the Binding Modes of Pyrethroid Insecticides with Voltage-Gated Sodium Channels in the Plant Bug Lygus pratensis (Hemiptera: Miridae) DOI
Liqi Zhang,

Ruoyao Ni,

Jing Chen

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 3, 2024

This study identified genetic mutations linked to resistance pyrethroid insecticides in the plant pest Lygus pratensis. The voltage-gated sodium channel (VGSC) gene was cloned, revealing two (Met918Thr and Leu1014Phe) laboratory strains field populations from Inner Mongolia, resulting variable resistance. A 3D model of LpVGSC created using homology modeling, binding patterns were analyzed via molecular docking. Molecular dynamics simulations confirmed structural stability changes pyrethroids VGSC sites. Mutation frequencies homozygous heterozygous genotypes did not exceed 40 20%, respectively. Toxicity tests showed high λ-cyhalothrin (LC50:401.31 ng/cm2). kdr (L1014F) superkdr (M918T) weakened interaction forces, reducing binding. M918T L1014F are predicted reduce Type I affinity, suggesting II may be more effective against resistant strains. These findings aid management insecticide design.

Language: Английский

Citations

2
Functional Analysis of Two Carboxylesterase Genes Involved in Beta-Cypermethrin and Phoxim Resistance in Plutella xylostella (L.) DOI Creative Commons
Ran Li, Liang Liang, Yaohua Zhao

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(12), P. 2781 - 2781

Published: Nov. 23, 2024

Enhanced expression of carboxylesterase (CarE) genes is an important mechanism insecticide resistance in pests. However, their roles multi-insecticide have rarely been reported. Herein, two CarE (PxαE6 and PxαE9) were identified; relative levels three multi-insecticide-resistant populations P. xylostella (HN, GD-2017 GD-2019) 2.69- to 15.32-fold higher than those the sensitive population, they considerably overexpressed at larval stage midgut 4th instar. PxαE6 PxαE9 knockdown increased susceptibility GD-2019 larvae phoxim or/and beta-cypermethrin. The recombinant expressed Escherichia coli exhibited high hydrolysis activity towards α-NA. GC–MS LC–MS/MS assays revealed that could metabolize beta-cypermethrin with efficiency determinations 51.6% 21.1%, respectively, while 12.0%. Homology modelling, molecular docking molecular-dynamics simulation analyses demonstrated fit well into active pocket stably bind or PxαE9. These results show overexpression involved populations, a finding which sheds light on mechanisms insect

Language: Английский

Citations

1
A Tiny Sample Rapid Visual Detection Technology for Imidacloprid Resistance in Aphis Gossypii by Crispr/Cas12a DOI

Rujing Kang,

Ren Li,

Juma Mjengi

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Alanine to glycine substitution in the PyR2 confers sodium channel resistance to Type I pyrethroids DOI Open Access
Mengli Chen, Likui Wang,

Xiangyi Zhou

et al.

Pest Management Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 30, 2024

Abstract BACKGROUND Aedes aegypti is a primary urban vector of dengue, yellow fever, Zika and chikungunya worldwide. Pyrethroid insecticides are the most effective for controlling Ae. . However, pyrethroid resistance has developed due to long‐term overuse insecticides, many knockdown (kdr) mutations have been identified in resistant populations. A1007G, an alanine glycine substitution, was found from Vietnam Malaysia, which always co‐existed with F1534C V1016G. role A1007G linkage or V1016G remain unknown. RESULTS In this study, we examined effects on sodium channel gating properties sensitivity Xenopus oocytes. We + shifted voltage dependence activation depolarizing direction. Mutations inactivation Both reduced two Type I pyrethroids, permethrin bifenthrin, synergistic were observed between F1534C. none mutations, affected II deltamethrin cypermethrin. Furthermore, triple significantly both pyrethroids. CONCLUSION had distinct effect I, but not also exhibited will provide fundamental insight into molecular interactions insect © 2024 Society Chemical Industry.

Language: Английский

Citations

0