Microbiology Spectrum,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 21, 2024
ABSTRACT
Persistent
infection
with
high-risk
human
papillomavirus
(HR-HPV)
is
the
principal
etiological
factor
of
cervical
cancer.
Considering
gradual
progression
cancer,
early,
rapid,
sensitive,
and
specific
identification
HPV,
particularly
HR-HPV
types,
crucial
in
halting
advancement
illness.
Here,
we
established
a
highly
detection
platform,
leveraging
CRISPR/Cas12a
assay
conjunction
multienzyme
isothermal
rapid
amplification.
Our
platform
enables
genotyping
14
types
by
using
type-specific
crRNAs.
The
outcomes
can
be
interpreted
either
through
fluorescence
reader
or
visually.
Furthermore,
achieved
one-tube
multiplex
use
multiple
amplifications
crRNA
pool.
sensitivity
this
method
2
copies/μL
no
cross-reactivity,
results
obtained
within
30
minutes.
This
exhibited
100%
clinical
specificity
when
applied
to
258
specimens.
Based
on
these
findings,
our
CRISPR/Cas-based
holds
promise
as
novel
tool,
offering
visually
intuitive
expedited
alternative
existing
HPV
diagnostics
providing
fresh
perspectives
for
cancer
screening.
IMPORTANCE
study
developed
based
technology.
not
only
but
also
achieves
single-tube
ingenious
design.
To
best
knowledge,
first
paper
utilize
CRISPR/Cas
diagnostic
technology
simultaneous
evaluate
its
feasibility
sample
large
number
samples.
We
hope
that
work
will
facilitate
accurate
promote
broader
application
Lab on a Chip,
Journal Year:
2024,
Volume and Issue:
24(5), P. 1441 - 1493
Published: Jan. 1, 2024
This
review
explores
emerging
microfluidic-based
technologies
incorporating
innovative
assay
strategies
for
infectious
disease
diagnostics.
Our
classification
scheme
is
based
on
the
human
body
systems
or
pathogen
transmission
modes.
ACS Sensors,
Journal Year:
2023,
Volume and Issue:
8(12), P. 4420 - 4441
Published: Nov. 18, 2023
CRISPR/Cas
system
is
becoming
an
increasingly
influential
technology
that
has
been
repositioned
in
nucleic
acid
detection.
A
preamplification
step
usually
required
to
improve
the
sensitivity
of
CRISPR/Cas-based
The
striking
biological
features
CRISPR/Cas,
including
programmability,
high
and
sequence
specificity,
single-base
resolution.
More
strikingly,
target-activated
trans-cleavage
could
act
as
a
biocatalytic
signal
transductor
amplifier,
thereby
empowering
it
potentially
perform
detection
without
step.
reports
such
work
are
on
rise,
which
not
only
scientifically
significant
but
also
promising
for
futuristic
end-user
applications.
This
review
started
with
introduction
methods
acids
diagnostics
(CRISPR-Dx).
Next,
we
objectively
discussed
pros
cons
steps
CRISPR-Dx.
We
then
illustrated
highlighted
recently
developed
strategies
CRISPR/Cas-powered
amplification-free
can
be
realized
through
uses
ultralocalized
reactors,
cascade
reactions,
ultrasensitive
systems,
or
others.
Lastly,
challenges
perspectives
were
proposed.
It
expected
this
makes
researchers
better
understand
current
emerging
field,
provides
insight
designing
novel
CRISPR-Dx
win
practicable
use
near
future.
Small,
Journal Year:
2024,
Volume and Issue:
20(24)
Published: March 20, 2024
Abstract
The
development
of
novel
method
for
drug‐resistant
bacteria
detection
is
imperative.
A
simultaneous
dual‐gene
Test
methicillin‐resistant
Staphylococcus
aureus
(MRSA)
developed
using
an
Argonaute‐centered
portable
biosensor
(STAR).
This
the
first
report
concerning
Argonaute‐based
pathogenic
detection.
Simply,
species‐specific
mec
and
nuc
gene
are
isothermally
amplified
loop‐mediated
isothermal
amplification
(LAMP)
technique,
followed
by
enabled
its
programmable,
guided,
sequence‐specific
recognition
cleavage.
With
strategy,
targeted
nucleic
acid
signals
dexterously
converted
into
fluorescent
signals.
STAR
capable
detecting
mecA
simultaneously
in
a
single
reaction.
limit
10
CFU/mL
with
dynamic
range
from
to
7
CFU/mL.
sample‐to‐result
time
<65
min.
successfully
adapted
detect
clinical
samples,
contaminated
foods,
MRSA‐infected
animals.
work
broadens
reach
biosensing
presents
bacterial
point‐of‐need
(PON)
platform.
