Biodegradation of chemical contamination by lactic acid bacteria: A biological tool for food safety

Food Chemistry, Journal Year: 2024, Volume and Issue: 460, P. 140732 - 140732

Published: Aug. 2, 2024

Language: Английский

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Melatonin Alleviates T-2 Toxin-Induced Intestinal Injury by Enhancing Gut Barrier Function and Modulating Microbiota in Weaned Piglets

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

The T-2 toxin, originating from a Fusarium species, is mycotoxin that can adversely affect animal health. Melatonin (MT) natural hormone recognized for its properties reduce inflammation and act as an antioxidant. However, MT's capacity to alleviate intestinal harm toxin remains incompletely explored. Employing postweaning piglets, this research investigates prophylactic impact on toxin-induced enterotoxicity. results indicate MT improved growth performance in piglets exposed toxins while also enhancing barrier function. Such effects probably stem ability colonic oxidative stress inflammation. Further findings suggest these changes are closely associated with MT-induced remodeling of microbiota increase short-chain fatty acid (SCFA) levels the intestine. therefore alleviates damage; gut key process.

Language: Английский

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The role of gut microbiota in anorexia induced by T-2 toxin

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 281, P. 116612 - 116612

Published: June 18, 2024

T-2 toxin is one of trichothecene mycotoxins, which can impair appetite and decrease food intake. However, the specific mechanisms for toxin-induced anorexia are not fully clarified. Multiple research results had shown that gut microbiota have a significant effect on regulation. Hence, this study purposed to explore potential interactions regulate factors in induced by toxin. The divided mice into control group (CG, 0 mg/kg BW toxin) toxin-treated (TG, 1 toxin), oral gavage 4 weeks, construct subacute poisoning mouse model. This data proved was able induce an increased contents gastrointestinal hormones (CCK, GIP, GLP-1 PYY), neurotransmitters (5-HT SP), as well pro-inflammatory cytokines (IL-1β, IL-6 TNF-α) serum mice. disturbed composition microbiota, especially, Faecalibaculum Allobaculum, positively correlated with CCK, GLP-1, 5-HT, IL-1β, TNF-α, played certain role regulating host appetite. In conclusion, changes (especially increase abundance Allobaculum) promote upregulation hormones, neurotransmitters, cytokines, may be mechanism anorexia.

Language: Английский

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Selenomethionine alleviates T-2 toxin-induced articular chondrocyte ferroptosis via the system Xc−/GSH/GPX4 axis

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 290, P. 117569 - 117569

Published: Dec. 18, 2024

Language: Английский

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Preparation of rabbit monoclonal antibody against T-2 toxin and development of enzyme-linked immunosorbent assay in milk, feed and pork samples

Microchemical Journal, Journal Year: 2024, Volume and Issue: unknown, P. 111977 - 111977

Published: Oct. 1, 2024

Language: Английский

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Citrinin-Induced Intestinal Onset of Pyroptosis via the IP3R1–GRP75–VDAC1 Complex-Mediated Mitochondrial Oxidative Stress

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Citrinin (CTN) is commonly found in animal feed and stored grains poses a serious threat to human health. Formation of the IP3R1–GRP75–VDAC1 complex has been shown play key role intestinal defense against harmful stimuli, but mechanism its action CTN-exposure-induced enterotoxicity not clear. Therefore, aim this study was investigate IPEC-J2 monolayer cell damage mice. It that CTN exposure triggered pyroptosis increased formation as well mitochondrial levels calcium ions reactive oxygen species (mtROS). And mtROS considered be factor cellular pyroptosis. removal by using Mito-Tempo attenuate failed ion overload. However, silencing GRP75 alleviated increases level mtROS, ions, subsequent confirms induces juxtaposition tissues points out oxidative stress mediated which triggers

Language: Английский

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Biosynthesis of the Central Tricyclic Skeleton of Trichothecene Mycotoxins

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Trichothecenes are a widespread family of sesquiterpenoid toxins that can pose significant risks to food and feed safety as well environmental health. A defining feature all trichothecenes is their central tricyclic 12,13-epoxytrichothec-9-ene (EPT) motif. Although the formation EPT skeleton has long been presumed be spontaneous process, nonenzymatic cyclization reaction forming tetrahydropyran ring in requires acid catalysis; otherwise, it occurs too slowly sustain efficient trichothecene biosynthesis under physiological conditions. Here, we resolved this decades-old problem by identifying missing enzymes for biosynthesis. We demonstrate C11 hydroxyl group universal precursors, isotrichodiol isotrichotriol, must acetylated strictly conserved O-acetyltransferase Tri3 furnish better leaving group. These intermediates preferentially undergo allylic rearrangement with water give shunt products, trichodiol trichotriol. Therefore, novel cyclase, Tri14, which was previously annotated hypothetical protein, required overcome kinetically unfavored oxide bridge closure meanwhile suppress any products.

Language: Английский

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Biodegradation of T‐2 Toxin by a Dominant Microbial Consortium Isolated From Chinese Yeast Ball

eFood, Journal Year: 2025, Volume and Issue: 6(2)

Published: March 12, 2025

ABSTRACT T‐2 toxin is a highly toxic type A trichothecene mycotoxin commonly found in feed, agricultural products like wheat, corn, and barley, as well processed foods, presenting substantial health hazards to both humans animals. In this study, microbial consortium (designated BJ) with stable efficient degradation activity was isolated from Chinese yeast balls, traditional fermentation starter. The dominated by species of Wickerhamomyces , Pichia Pediococcus . This BJ demonstrated the ability degrade 99.2% HT‐2 toxin, triol, tetraol within 48 h, outperforming individual strains. Metagenomic sequencing LC‐MS/MS metabolic analysis elucidated pathway, which includes deacetylation de‐epoxidation epoxy group, cytochrome P450 enzyme CYP61 ciferrii playing key role detoxification. study highlights potential natural agent for detoxification feed food industry, though further research warranted assess its real‐world application safety.

Language: Английский

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T-2 toxin induces anemia via disrupting erythroid commitment and differentiation

Toxicology, Journal Year: 2025, Volume and Issue: unknown, P. 154126 - 154126

Published: March 1, 2025

Language: Английский

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Natural Phenolic Compounds against Trichothecenes: From Protective Mechanisms to Future Perspectives

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Trichothecenes (TCNs), Fusarium-derived mycotoxins exemplified by deoxynivalenol and T-2 toxin, threaten global health through multisystem toxicity widespread contamination. Natural phenolic compounds (NPCs), leveraging their intrinsic safety natural abundance, demonstrate multimechanistic efficacy in counteracting TCN toxicity. This article reviews both domestic international research on the protective mechanisms of NPCs against TCN-induced exert effects multitiered mechanisms: (1) molecular regulation via Nrf2-centric antioxidant activation MAPK/NF-κB inflammatory axis suppression, coupled with coordinated inhibition programmed cell death pathways (apoptosis/ferroptosis/pyroptosis) autophagy modulation, where GPX4 emerges as a critical ferroptosis regulator; (2) restoring microbiome balance, enhancing intestinal barrier function, optimizing nutrient transport. Gut microflora may also serve an additional target for mitigating TCNs. further inhibit Fusarium proliferation mycotoxin biosynthesis. While there is demonstrated potential food sustainable feed development, challenges persist bioavailability optimization, pharmacokinetic profiling, microbiota-metabolite crosstalk. analysis advances NPC-based strategies detoxification agriculture.

Language: Английский

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