De novo biosynthesis of mogroside V by multiplexed engineered yeasts

Metabolic Engineering, Journal Year: 2025, Volume and Issue: 88, P. 160 - 171

Published: Jan. 7, 2025

Language: Английский

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Recent Advances in the Distribution, Chemical Composition, Health Benefits, and Application of the Fruit of Siraitia grosvenorii

Foods, Journal Year: 2024, Volume and Issue: 13(14), P. 2278 - 2278

Published: July 19, 2024

The fruits of Siraitia grosvenorii (S. grosvenorii) have attracted a lot scientific interest as part the current healthy diet. S. has diverse health-promoting effects, including antioxidant, anti-inflammatory, antimicrobial, respiratory modulation, metabolic antitumor, and neuroprotective well gastrointestinal function modulation. As plant resource, broad application prospects, which promotes development horticultural industry. Moreover, Mogroside much attention an important active ingredient grosvenorii. This review provides in-depth exploration distribution, chemical composition, health benefits, grosvenorii, particularly Mogroside. comprehensive highlights therapeutic potential prompting further research into its applications. value-added functional ingredients, constituents significant for disease prevention are widely used in food supplements.

Language: Английский

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The Past, Present and Future of Plant Activator Targeting Salicylic Acid Signal Pathway

Published: July 17, 2024

Plant activators have emerged as promising alternatives to conventional pesticides for crop disease manage-ment due their unique mode of action. By priming the plant's innate immune system, these compounds can induce systemic acquired resistance against a broad spectrum pathogens without directly inhibiting proliferation. Key advantages plant include prolonged defense activity, lower effective dosages, and negligible risk developing pathogen resistance. Among various defensive pathways tar-geted, salicylic acid (SA) signaling cascade has been extensively explored, leading successful devel-opment commercial like benzothiadiazole widespread application in protection. While action sites many SA-targeting preliminarily mapped different steps along pathway, comprehensive understanding precise mechanisms remains elusive. This review provides historical perspective on activator development outlines diverse screening strategies employed across multiple levels, from whole-plant bioassays molecular transgenic approaches. We expound intricate components, biological relevance, regulatory circuits governing SA while criti-cally examining structural features, bioactivities, proposed modes classical such derivatives, analogs, other small molecules. Insights field trials as-sessing practical applicability are also discussed. Furthermore, we highlight current status, challenges, future prospects realm globally, with focus recent en-deavors China. Collectively, this aims synthesize existing knowledge provide roadmap research toward more potent mechanistically understood activa-tors that fortify immunity disease.

Language: Английский

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Rationally Engineered Novel Glycosyltransferase UGT74DD1 from Siraitia grosvenorii Catalyzes the Generation of the Sweetener Mogroside III

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(32), P. 18214 - 18224

Published: Aug. 5, 2024

Mogrosides are natural compounds highly valued in the food sector for their exceptional sweetness. Here, we report a novel O-glycosyltransferase (UGT74DD1) from Siraitia grosvenorii that catalyzes conversion of mogrol to mogroside IIE. Site-directed mutagenesis yielded UGT74DD1-W351A mutant, which exhibited new capability transform IIE into valuable sweetener III, but with low catalytic activity. Subsequently, using structure-guided directed evolution combinatorial active-site saturation testing, superior mutant M6 (W351A/Q373 K/E49H/Q335W/S278C/D17F) were obtained, showed 46.1-fold increase activity compared UGT74DD1-W351A. Molecular dynamics simulations suggested enhanced and extended substrate profiles due its enlarged substrate-binding pocket strengthened enzyme–substrate hydrogen bonding interactions. Overall, redesigned UGT74DD1, yielding mutants catalyze III. This study thus broadens toolbox UGTs capable catalyzing formation polyglycoside compounds.

Language: Английский

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Hypoglycemic activity of enzymatically extracted Eucommia ulmoides polysaccharide (EUL-w1) on IR-HepG2 cell via the AMPK/PI3K/Akt signaling pathway

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 283, P. 137596 - 137596

Published: Nov. 13, 2024

Language: Английский

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Anti-glycation activity and mechanism of Siraitia grosvenorii polysaccharide based on bovine serum albumin-fructose and Caco-2 cell models

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142267 - 142267

Published: March 1, 2025

Language: Английский

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Molecular characterization and structural basis of a promiscuous glycosyltransferase for β‐(1,6) oligoglucoside chain glycosides biosynthesis

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

Summary Sugar building blocks are crucial for the chemical diversity and biological activity of secondary metabolites. UDP‐dependent glycosyltransferases (UGTs) play a pivotal role in biosynthesis glycosides plants by catalysing attachment sugar moieties to various bioactive natural products. However, oligosaccharide‐chain is often limited narrow substrate specificity UGTs. In this study, we identify regio‐specific β ‐(1,6) glycosyltransferase, UGT94BY1, from Platycodon grandiflorum . UGT94BY1 exhibits broad promiscuity can transfer up three C6‐OH position glucosyl group triterpenoids phenolic glycosides, thereby forming oligoglucoside chains. To elucidate mechanism underlying its selectivity, determined crystal structure complex with UDP at resolution 2.0 Å. Molecular simulations revealed that critical structural motif, comprising residues N84‐M91, S141‐L155 R179‐E186, plays key recognizing acceptors facilitating chain elongation. Our study unveils powerful glycosyltransferase highlights regions involved recognition extension, providing valuable insights designing UGTs customized specificities biotechnological applications.

Language: Английский

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Spatial variability and climate response characteristics of the chemical components in Siraitia grosvenorii (Luo Han Guo)

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 228, P. 120897 - 120897

Published: March 31, 2025

Language: Английский

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The Potential of Siraitia grosvenorii to Promote Bone Regeneration via Modulating Macrophage Polarization: A Network Pharmacology and Experimental Study

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3609 - 3609

Published: April 11, 2025

Siraitia grosvenorii (SG), a traditional Chinese medicinal herb, possesses immunomodulatory and osteoinductive properties, yet its pharmacological mechanisms in bone defect repair remain largely unexplored. This study investigates the therapeutic potential of SG through combination network pharmacology experimental approaches. Active compounds were identified using Traditional Medicine Systems Pharmacology (TCMSP) Platform, protein interaction targets predicted. Molecular docking dynamics simulations assessed interactions between critical targets. In vitro, RAW 264.7 macrophages treated with SG-conditioned medium exhibited enhanced M2 polarization reduced inflammation, promoting osteogenic differentiation co-cultured MC3T3-E1 cells as evidenced by increased alkaline phosphatase activity. vivo, scaffolds loaded low-dose or high-dose (LSG/HSG) significantly improved regeneration rat calvarial defects, HSG achieving near-complete mature trabeculae at 8 weeks, alongside decreased CD86 TNF-α levels IL-10 expression. Network 33 shared related to macrophage polarization, kaempferol beta-sitosterol demonstrating strong binding affinities such TNF, PTGS2, CASP3. These findings highlight enhancing implications for regenerative medicine.

Language: Английский

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Exogenous Mogroside V Drove Microbial Carbohydrate Metabolism and Consequently Enhanced Fruity Aroma in Greengage Wine

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

The microbial community is essential for the formation of aroma development in high-acidity greengage wine fermentation. Recent observations also highlight positive effects mogroside V (MG V) on microorganisms fermented foods, but underlying chemical and biological mechanisms remain inadequate. results indicated differences physicochemical properties among wines, particularly a 50% increase ethanol conversion rate. Concurrently, GC-MS sensory analyses demonstrated that MG augmented carbohydrate into ethyl esters (twice as much control group), exhibiting tropical fruit floral profiles. perceived intensity these aromatic compounds increased by over 30%, thereby enriching overall harmony wine. Integrated analysis KEGG pathways CAZymes annotations enhancement ester depends improvement transport carbohydrates V, well accelerating flux pyruvate to acetyl-CoA yeast. In conclusion, our study presents targeted strategy industry modulating this metabolic node yeast, achieving focused characteristics wines.

Language: Английский

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