Ferritin Nanocage-Enabled Detection of Pathological Tau in Living Human Retinal Cells DOI Creative Commons
Lorenzo Barolo,

Ylenia Gigante,

Lorenza Mautone

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 8, 2024

Abstract Background Tauopathies, such as Alzheimer's disease and Frontotemporal Dementia, are debilitating neurodegenerative disorders characterized by cognitive decline. Despite extensive research, effective treatments significant advancements in managing symptoms have been challenging to achieve. Accurate diagnosis is critical for developing therapeutic strategies. Hyperphosphorylated protein units tau oligomers recognized reliable biomarkers these conditions. This study introduces an innovative approach using nanotechnology enhance the diagnostic process tauopathies. We focus on development application of humanized ferritin nanocages, a novel nanoscale delivery system, designed encapsulate transport tau-specific fluorophore, BT1, into human retinal cells, detection neurofibrillary tangles tissue, key marker Results The BT1 living cells was achieved through use system at nanoscale. nanocages demonstrated efficient encapsulation derived from induced pluripotent stem cells. Our experiments successful colocalization with pathological forms highlighting potential this method identifying Conclusions employment probe represents important contribution field nanobiotechnology, especially context diagnostics. offers promising tool early implications improving management exemplifies integration biomedical science, expanding frontiers nanomedicine techniques.

Language: Английский

Combined strategies for optimizing BODIPY-based near-infrared fluorescent probe targeting Aβ oligomers DOI

Takahiro Akasaka,

Hiroyuki Watanabe,

Masahiro Ono

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 417, P. 136132 - 136132

Published: June 11, 2024

Language: Английский

Citations

5
Iterative Design of Near-Infrared Fluorescent Probes for Early Diagnosis of Alzheimer’s Disease by Targeting Aβ Oligomers DOI
Bing Liu, Xiaofang Li,

Zhengyang Liu

et al.

Journal of Medicinal Chemistry, Journal Year: 2024, Volume and Issue: 67(11), P. 9104 - 9123

Published: June 3, 2024

Amyloid-β oligomers (AβOs), crucial toxic proteins in early Alzheimer's disease (AD), precede the formation of Aβ plaques and cognitive impairment. In this context, we present our iterative process for developing novel near-infrared fluorescent (NIRF) probes specifically targeting AβOs, aimed at AD diagnosis. An initial screening identified compound

Language: Английский

Citations

4
Novel amyloid-β oligomer-targeted fluorescent probes DOI

Rikuto Kashiyama,

Hiroyuki Watanabe,

Takahiro Akasaka

et al.

Dyes and Pigments, Journal Year: 2025, Volume and Issue: unknown, P. 112680 - 112680

Published: Jan. 1, 2025

Citations

0
Smart fluorogenic tools: From designing principles to visualization of multistep protein aggregation DOI

Jingyang Wan,

Chen‐Yang Huang, Ziyue Chen

et al.

Coordination Chemistry Reviews, Journal Year: 2025, Volume and Issue: 535, P. 216625 - 216625

Published: March 19, 2025

Language: Английский

Citations

0
Methods for high throughput discovery of fluoroprobes that recognize tau fibril polymorphs DOI Creative Commons
Emma C. Carroll, Hyunjun Yang, Julia P. G. Jones

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 2, 2024

Aggregation of microtubule-associated protein tau (MAPT/tau) into conformationally distinct fibrils underpins neurodegenerative tauopathies. Fluorescent probes (fluoroprobes), such as thioflavin T (ThT), have been essential tools for studying aggregation; however, most them do not discriminate between amyloid fibril conformations (polymorphs). This gap is due, in part, to a lack high-throughput methods screening large, diverse chemical collections. Here, we leverage advances adaptive differential scanning fluorimetry (paDSF) screen the Aurora collection 300+ fluorescent dyes against multiple synthetic polymorphs. screen, coupled with orthogonal secondary assays, revealed pan-fibril binding chemotypes, well fluoroprobes selective subsets fibrils. One fluoroprobe recognized pathology

Language: Английский

Citations

3
Near-infrared fluorescent probes for imaging of amyloid-β at different stages in monitoring Alzheimer's disease DOI Creative Commons

Xueqi Zhao,

Ruiwen Zhang,

Dexiang Hu

et al.

Dyes and Pigments, Journal Year: 2024, Volume and Issue: 224, P. 111993 - 111993

Published: Jan. 28, 2024

As one of the expensive and heavy illnesses, Alzheimer's disease (AD) has plagued mankind for a long time. The deposition amyloid-β (Aβ) plaques in brain is AD's main early pathological characteristic, also growing body evidence shows Aβ good diagnostic AD. In recent years, near-infrared fluorescence (NIRF) imaging emerged with real-time, safe, low-cost technology as promising tool diagnosis. process development AD, gone through monomer, oligomer to insoluble fiber process. Therefore, it useful necessary develop NIRF probes that can specifically detect at various stages. this review, we summarized recognize different We focused on design strategies, optical properties, dynamics characteristics, discussed structural characteristics types these probes. believe review will provide some ideas

Language: Английский

Citations

2
Investigating the Potential Impact of Air Pollution on Alzheimer’s Disease and the Utility of Multidimensional Imaging for Early Detection DOI Creative Commons

Ankul Singh S,

Mohd Nazam Ansari,

Gehan M. Elossaily

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(8), P. 8615 - 8631

Published: Feb. 15, 2024

Pollution is ubiquitous, and much of it anthropogenic in nature, which a severe risk factor not only for respiratory infections or asthma sufferers but also Alzheimer's disease, has received lot attention recently. This Review aims to investigate the primary environmental factors their profound impact on disease. It underscores pivotal role multidimensional imaging early disease identification prevention. Conducting comprehensive review, we delved into plethora literature sources available through esteemed databases, including Science Direct, Google Scholar, Scopus, Cochrane, PubMed. Our search strategy incorporated keywords such as "Alzheimer Disease", "Alzheimer's", "Dementia", "Oxidative Stress", "Phytotherapy" conjunction with "Criteria Pollutants", "Imaging", "Pathology", "Particulate Matter". result complex biological exacerbated by infiltration airborne particles gases that surreptitiously breach nasal defenses traverse brain, akin Trojan horse. Various modalities noninvasive techniques have been harnessed identify progression its incipient stages. However, each approach possesses inherent limitations, prompting exploration unified technique under single umbrella. Multidimensional stands linchpin detecting forestalling relentless march Given intricate etiology condition, identifying prospective candidate may take decades, rendering development multimodal an imperative. research pressing need recognize chronic ramifications invisible particulate matter advance our understanding insidious contribute

Language: Английский

Citations

2
Red emissive fluorescent carbon dots based on ternary carbon source for imaging α-synuclein fibrils DOI
Jintao Zhang,

Luo Wan-chun,

Yu Zhang

et al.

Journal of Colloid and Interface Science, Journal Year: 2024, Volume and Issue: 670, P. 576 - 584

Published: May 18, 2024

Language: Английский

Citations

2
Ferritin nanocage-enabled detection of pathological tau in living human retinal cells DOI Creative Commons
Lorenzo Barolo,

Ylenia Gigante,

Lorenza Mautone

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 21, 2024

Tauopathies, including Alzheimer's disease and Frontotemporal Dementia, are debilitating neurodegenerative disorders marked by cognitive decline. Despite extensive research, achieving effective treatments significant symptom management remains challenging. Accurate diagnosis is crucial for developing therapeutic strategies, with hyperphosphorylated protein units tau oligomers serving as reliable biomarkers these conditions. This study introduces a novel approach using nanotechnology to enhance the diagnostic process tauopathies. We developed humanized ferritin nanocages, nanoscale delivery system, designed encapsulate transport tau-specific fluorophore, BT1, into human retinal cells detecting neurofibrillary tangles in tissue, key marker of The BT1 living was successfully achieved through demonstrating efficient encapsulation derived from induced pluripotent stem cells. Our experiments confirmed colocalization pathological forms cells, highlighting method's potential identifying Using nanocages represents contribution nanobiotechnology, particularly diagnostics. method offers promising tool early detection implications improving exemplifies integration biomedical science, expanding frontiers nanomedicine techniques.

Language: Английский

Citations

2
Development of BODIPY-based fluorescent probes for imaging Aβ aggregates and lipid droplet viscosity DOI

Kaibo Li,

Yuxuan Wang,

Yanping Li

et al.

Talanta, Journal Year: 2024, Volume and Issue: 277, P. 126362 - 126362

Published: June 5, 2024

Language: Английский

Citations

2