Non–steady state thermometry with optical diffraction tomography DOI Creative Commons
Adarsh B. Vasista, Bernard Ciraulo, Falko Schmidt

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(12)

Published: March 22, 2024

Label-free thermometry is a pivotal tool for many disciplines. However, most current approaches are only suitable planar heat sources in steady state, thereby restricting the range of systems that can be reliably studied. Here, we introduce pump probe-based optical diffraction tomography (ODT) as method to map temperature precisely and accurately three dimensions (3D) at single-particle level. To do so, first systematically characterize thermal landscape model system consisting gold nanorods microchamber then benchmark results against simulations quantitative phase imaging thermometry. We apply ODT resolve landscapes inaccessible other label-free form nonplanar embedded complex environments freely diffusing microchamber. Last, foresee our approach will find applications where routine characterization heterogeneous nanoparticles samples 3D or non-steady state required.

Language: Английский

Single-cell mapping of lipid metabolites using an infrared probe in human-derived model systems DOI Creative Commons
Yeran Bai,

Carolina M. Camargo,

Stella M.K. Glasauer

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 8, 2024

Abstract Understanding metabolic heterogeneity is the key to uncovering underlying mechanisms of metabolic-related diseases. Current imaging studies suffer from limitations including low resolution and specificity, model systems utilized often lack human relevance. Here, we present a single-cell platform enable direct lipid metabolism with high specificity in various human-derived 2D 3D culture systems. Through incorporation an azide-tagged infrared probe, selective detection newly synthesized lipids cells tissue became possible, while simultaneous fluorescence enabled cell-type identification complex tissues. In proof-of-concept experiments, were directly visualized human-relevant among different cell types, mutation status, differentiation stages, over time. We identified upregulated progranulin-knockdown induced pluripotent stem their differentiated microglia cells. Furthermore, observed that neurons brain organoids exhibited significantly lower compared astrocytes.

Language: Английский

Citations

24
Video-rate mid-infrared photothermal imaging by single-pulse photothermal detection per pixel DOI Creative Commons
Jiaze Yin, Meng Zhang, Yuying Tan

et al.

Science Advances, Journal Year: 2023, Volume and Issue: 9(24)

Published: June 14, 2023

By optically sensing absorption-induced photothermal effect, mid-infrared (IR) (MIP) microscope enables super-resolution IR imaging of biological systems in water. However, the speed current sample-scanning MIP system is limited to milliseconds per pixel, which insufficient for capturing living dynamics. detecting transient signal induced by a single pulse through fast digitization, we report laser-scanning that increases three orders magnitude. To realize single-pulse detection, use synchronized galvo scanning both mid-IR and probe beams achieve an line rate more than 2 kilohertz. With video-rate speed, observed dynamics various biomolecules organisms at multiple scales. Furthermore, using hyperspectral imaging, chemically dissected layered ultrastructure fungal cell wall. Last, with uniform field view 200 square micrometer, mapped fat storage free-moving

Language: Английский

Citations

33
Mapping enzyme activity in living systems by real-time mid-infrared photothermal imaging of nitrile chameleons DOI
Hongjian He, Jiaze Yin, Mingsheng Li

et al.

Nature Methods, Journal Year: 2024, Volume and Issue: 21(2), P. 342 - 352

Published: Jan. 8, 2024

Language: Английский

Citations

13
VIBRANT: spectral profiling for single-cell drug responses DOI
Xinwen Liu, Lixue Shi, Zhilun Zhao

et al.

Nature Methods, Journal Year: 2024, Volume and Issue: 21(3), P. 501 - 511

Published: Feb. 19, 2024

Language: Английский

Citations

11
Coherent Anti-Stokes Hyper-Raman Spectroscopy DOI Creative Commons
Kazuki Inoue, Masanari Okuno

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 10, 2025

Coherent Raman scattering spectroscopies have been established as a powerful tool for investigating molecular systems with high chemical specificity. The existing coherent techniques detect only active modes, which are part of the whole vibrations. Here, we report first observation anti-Stokes hyper-Raman (CAHRS) spectroscopy, allows measuring vibrations at speed. CAHRS process relies on fifth-order nonlinear that combines scattering. Observed signals proven to come from through various experiments concerning dependences incident laser powers, time-delay, polarizations, and selection rules Comparisons spontaneous para-nitroaniline solutions benzene liquid manifest much higher signal-to-noise ratios than signals. This study illustrates spectroscopy can offer additional information unobtainable present speed spectroscopy. authors It spectra

Language: Английский

Citations

1
Bioaerosol Characterization with Vibrational Spectroscopy: Overcoming Fluorescence with Photothermal Infrared (PTIR) Spectroscopy DOI
Jia H. Shi,

Carlie J. Poworoznek,

Rebecca L. Parham

et al.

The Journal of Physical Chemistry A, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Aerosols containing biological material (i.e., bioaerosols) impact public health by transporting toxins, allergens, and diseases the climate nucleating ice crystals cloud droplets. Single particle characterization of primary aerosol particles (PBAPs) is essential, as individual physicochemical properties determine their impacts. Vibrational spectroscopies, such infrared (IR) or Raman spectroscopy, provide detailed information about components within atmospheric aerosols but these techniques have traditionally been limited due to diffraction limit IR radiation (particles >10 μm) fluorescence bioaerosol overwhelming signal. Herein, we use photothermal spectroscopy (PTIR) overcome limitations characterize PBAPs down 0.18 μm. Both optical-PTIR (O-PTIR) atomic force microscopy-PTIR (AFM-PTIR) were used generated from a cyanobacterial harmful algal bloom (cHAB) dominated Planktothrix agardhii. PTIR spectra contained modes consistent with traditional Fourier transform (FTIR) for species, including amide I (1630-1700 cm-1) II (1530-1560 cm-1). The fractions materials greater in supermicron (1.8-3.2 than submicron (0.18-0.32 0.56-1.0 aerosolized cHAB water. These results demonstrate potential both O-PTIR AFM-PTIR studying range bioaerosols vibrational spectroscopy.

Language: Английский

Citations

1
Mid-Infrared Photonic Sensors: Exploring Fundamentals, Advanced Materials, and Cutting-Edge Applications DOI Creative Commons
Muhammad A. Butt, Marcin Juchniewicz, Mateusz Słowikowski

et al.

Sensors, Journal Year: 2025, Volume and Issue: 25(4), P. 1102 - 1102

Published: Feb. 12, 2025

Mid-infrared (MIR) photonic sensors are revolutionizing optical sensing by enabling precise chemical and biological detection through the interrogation of molecules' unique vibrational modes. This review explores core principles MIR photonics, emphasizing light-matter interactions within 2-20 µm wavelength range. Additionally, it examines innovative sensor architectures, such as integrated platforms fibers, that enhance sensitivity, specificity, device miniaturization. The discussion extends to groundbreaking applications in environmental monitoring, medical diagnostics, industrial processes, security, highlighting transformative impact these technologies. comprehensive overview aims illuminate current state-of-the-art while inspiring future developments sensing.

Language: Английский

Citations

1
Absolute Stimulated Raman Cross Sections of Molecules DOI
Xin Gao, Xuemeng Li, Wei Min

et al.

The Journal of Physical Chemistry Letters, Journal Year: 2023, Volume and Issue: 14(24), P. 5701 - 5708

Published: June 15, 2023

Stimulated Raman scattering (SRS) is a fundamental optical process that was discovered more than 60 years ago. While the early SRS spectroscopy studies have provided valuable insights into materials systems, advent of microscopy has launched rapidly growing field in biological imaging. However, understanding molecular response under still lacking. Herein we present new framework to introduce molecule-intrinsic stimulated cross sections, σSRS, unit Göppert-Mayer (GM). The absolute sections determined for real systems challenge conventional wisdom always weak process. enormous rate acceleration SRS, captured by an apparent section, stems from synergistic effect between and molecule. Our goes beyond optics-centric view presents molecule-inclusive perspective, thus offering comprehensive foundation future growth microscopy.

Language: Английский

Citations

19
A tutorial on optical photothermal infrared (O-PTIR) microscopy DOI Creative Commons
Craig Prater, Mustafa Kansiz, Ji‐Xin Cheng

et al.

APL Photonics, Journal Year: 2024, Volume and Issue: 9(9)

Published: Sept. 1, 2024

This tutorial reviews the rapidly growing field of optical photothermal infrared (O-PTIR) spectroscopy and chemical imaging. O-PTIR is an super-resolution measurement technique where a shorter wavelength visible probe used to measure map (IR) absorption with spatial resolution up 30× better than conventional techniques such as Fourier transform direct IR laser imaging systems. article key limitations instruments, technology breakthroughs, their origins that have overcome prior limitations. also discusses recent developments in expanding multi-modal approaches enable complementary Raman fluorescence microscopy imaging, including wide-field fluorescence-based detection absorption. Various practical subjects are covered, sample preparation techniques, optimal configurations, use tags/labels for data analysis, visualization. Key applications reviewed many areas, biological biomedical sciences, environmental microplastics research, (bio)pharmaceuticals, materials science, cultural heritage, forensics, photonics, failure analysis.

Language: Английский

Citations

8
Single 5-nm quantum dot detection via microtoroid optical resonator photothermal microscopy DOI Creative Commons
Shuang Hao, Sartanee Suebka, Judith Su

et al.

Light Science & Applications, Journal Year: 2024, Volume and Issue: 13(1)

Published: Aug. 19, 2024

Label-free detection techniques for single particles and molecules play an important role in basic science, disease diagnostics, nanomaterial investigations. While fluorescence-based methods are tools molecule imaging, they limited by available molecular probes photoblinking photobleaching. Photothermal microscopy has emerged as a label-free imaging technique capable of detecting individual nanoabsorbers with high sensitivity. Whispering gallery mode (WGM) microresonators can confine light small volume enhanced light-matter interaction thus promising ultra-sensitive photothermal platform. Previously, microtoroid optical resonators were combined to detect 250 nm long gold nanorods 100 polymers. Here, we combine microtoroids spatially 5 diameter quantum dots (QDs) signal-to-noise ratio exceeding 10

Language: Английский

Citations

7