[Research Progress in the Molecular Regulatory Mechanisms of Alveolar Bone Restoration]. DOI
Jieya Wei, Siqun Xu, Xuedong Zhou

et al.

PubMed, Journal Year: 2024, Volume and Issue: 55(1), P. 31 - 38

Published: Jan. 20, 2024

Alveolar bone, the protruding portion of maxilla and mandible that surrounds roots teeth, plays an important role in tooth development, eruption, masticatory performance. In oral inflammatory diseases, including apical periodontitis, peri-implantitis, alveolar bone defects cause loosening or loss impair function, endanger physical mental health patients. However, restoration is confronted with great clinical challenges due to complicated effect biological, mechanical, chemical factors microenvironment. An in-depth understanding underlying molecular regulatory mechanisms will contribute exploration new targets for restoration. Recent studies have shown Notch, Wnt, Toll-like receptor (TLR), nuclear factor-κB (NF-κB) signaling pathways regulate proliferation, differentiation, apoptosis, autophagy osteoclasts, osteoblasts, osteocytes, periodontal ligament cells, macrophages, adaptive immune modulate expression mediators, affect balance activator ligand/receptor factor-κB/osteoprotegerin (RANKL/RANK/OPG) system, ultimately participate Additionally, involves AMP-activated protein kinase (AMPK), phosphatidyl inositol 3-kinase/protein B (PI3K/AKT), Hippo/YAP, Janus kinase/signal transducer transcription (JAK/STAT), transforming growth factor β (TGF-β) pathways. current failed construct mature networks There urgent need further research on by using technologies such as single-cell transcriptome sequencing spatial sequencing.

Language: Английский

Annexin A5 derived from matrix vesicles protects against osteoporotic bone loss via mineralization DOI Creative Commons
Guanyue Su, Demao Zhang, Tiantian Li

et al.

Bone Research, Journal Year: 2023, Volume and Issue: 11(1)

Published: Nov. 9, 2023

Abstract Matrix vesicles (MVs) have shown strong effects in diseases such as vascular ectopic calcification and pathological calcified osteoarthritis wound repair of the skeletal system due to their membranous vesicle characteristics abundant calcium phosphorus content. However, role MVs progression osteoporosis is poorly understood. Here, we report that annexin A5, an important component matrix membrane, plays a vital bone homeostasis deterioration osteoporosis. We first identified A5 from adherent but not dissociative osteoblasts found it could be sharply decreased during occurrence based on ovariectomized mice. then confirmed its potential mediating mineralization precursor osteoblast lineage via initial binding with collagen type I achieve MV adhesion subsequent activation cellular autophagy. Finally, proved protective resisting loss by applying osteoporotic Taken together, these data revealed importance originating osteoblasts, remodeling provided new strategy for treatment intervention loss.

Language: Английский

Citations

14
Dentin Mechanobiology: Bridging the Gap between Architecture and Function DOI Open Access
Xiangting Fu, Hye Sung Kim

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 5642 - 5642

Published: May 22, 2024

It is remarkable how teeth maintain their healthy condition under exceptionally high levels of mechanical loading. This suggests the presence inherent adaptation mechanisms within structure to counter constant stress. Dentin, situated between enamel and pulp, plays a crucial role in mechanically supporting tooth function. Its intermediate stiffness viscoelastic properties, attributed its mineralized, nanofibrous extracellular matrix, provide flexibility, strength, rigidity, enabling it withstand loading without fracturing. Moreover, dentin’s unique architectural features, such as odontoblast processes dentinal tubules spatial compartmentalization odontoblasts dentin sensory neurons contribute distinctive perception external stimuli while acting defensive barrier for dentin-pulp complex. Since architecture governs functions nociception repair response stimuli, understanding mechanobiology developing treatments pain management dentin-associated diseases regeneration. review discusses physical features regulate mechano-sensing, focusing on mechano-sensitive ion channels. Additionally, we explore advanced vitro platforms that mimic providing deeper insights into fundamental mechanobiological phenomena laying groundwork effective mechano-therapeutic strategies diseases.

Language: Английский

Citations

4
FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes DOI Creative Commons
Minglei Huang, Haoran Chen,

Jieya Wei

et al.

Published: April 16, 2025

ABSTRACT Chondrocytes store lipids in the form of lipid droplets (LDs) and maintain cartilage metabolic homeostasis by consuming or regenerating LDs. This modulation is largely mediated a series biochemical factors. Fibroblast growth factor 8 (FGF8) one most important factors involved proliferation, differentiation, migration chondrocytes, has attracted increasing attention physiology pathology cartilage. However, effect FGF8 on LD accumulation chondrocytes remains unclear. study aimed to elucidate role LDs explore underlying biomechanism involved. The results showed that promoted upregulating perilipin1 (Plin1) expression. activates cytoplasmic p-p38 signaling pathway via fibroblast receptor 1 (FGFR1) increase chondrocytes. Subsequent experiments with siRNAs specific inhibitors further confirmed importance FGFR1/p38 axis for exposed FGF8. These our understanding provide insights into

Language: Английский

Citations

0
Biomimetic Fibers Based on Equidistant Micropillar Arrays Determines Chondrocyte Fate via Mechanoadaptability DOI Open Access
Chenchen Zhou,

Yueyi Yang,

Mengmeng Duan

et al.

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(30)

Published: Aug. 19, 2023

It is recognized that the changes in physical properties of extracellular matrix (ECM) result fine-tuned cell responses including morphology, proliferation and differentiation. In this study, a novel patterned equidistant micropillar substrate based on polydimethylsiloxane (PDMS) designed to mimic collagen fiber-like network cartilage matrix. By changing component curing agent an oligomeric base, substrates with same topology but different stiffnesses are obtained it found chondrocytes seeded onto soft maintain their phenotype by gathering type II aggrecan more effectively than those stiff substrate. Moreover, sense respond altering ECM-cytoskeleton-focal adhesion axis. Further, substrate-preserved chondrocyte dependent activation Wnt/β-catenin signaling. Finally, indicated osteoid-like region formation loss stiffened sclerotic area osteoarthritis validate triggered stiffnesses. This study provides behavior similar real at tissue level during transition from normal state osteoarthritis.

Language: Английский

Citations

10
The influence of microenvironment stiffness on endothelial cell fate: Implication for occurrence and progression of atherosclerosis DOI

Lin Cheng,

Hongyan Yue,

Huaiyi Zhang

et al.

Life Sciences, Journal Year: 2023, Volume and Issue: 334, P. 122233 - 122233

Published: Oct. 31, 2023

Language: Английский

Citations

10
PDGF‐AA guides cell crosstalk between human dental pulp stem cells in vitro via the PDGFR‐α/PI3K/Akt axis DOI
Siqun Xu, Jieya Wei, Yang Liu

et al.

International Endodontic Journal, Journal Year: 2024, Volume and Issue: 57(5), P. 549 - 565

Published: Feb. 9, 2024

Abstract Aim To explore the influence of PDGF‐AA on cell communication between human dental pulp stem cells (DPSCs) by characterizing gap junction intercellular (GJIC) and its potential biomechanical mechanism. Methodology Quantitative real‐time PCR was used to measure connexin family member expression in DPSCs. Cell migration CCK‐8 assays were utilized examine DPSC proliferation. A scrape loading/dye transfer assay applied evaluate GJIC triggered PDGF‐AA, a PI3K/Akt signalling pathway blocker (LY294002) PDGFR‐α (AG1296). Western blotting immunofluorescence test distribution Cx43 p‐Akt proteins Scanning electron microscopy (SEM) observe morphology Results promoted formation cells. upregulates enhance communication. binds activates regulate Conclusions This research demonstrated that can Cx43‐mediated DPSCs via PDGFR‐α/PI3K/Akt axis, which provides new cues for regeneration from perspective

Language: Английский

Citations

2
GroEL triggers NLRP3 inflammasome activation through the TLR/NF-κB p-p65 axis in human periodontal ligament stem cells DOI Creative Commons
Li Zhang, Mengmeng Duan,

Xiaohua Pu

et al.

Acta Biochimica et Biophysica Sinica, Journal Year: 2024, Volume and Issue: 56(9), P. 1340 - 1351

Published: April 10, 2024

The interaction between bacteria and the host plays a vital role in initiation progression of systemic diseases, including gastrointestinal oral due to secretion various virulence factors from these pathogens. GroEL, potent factor secreted by multiple pathogenic bacteria, is implicated damage gingival epithelium, periodontal ligament, alveolar bone other peripheral tissues. However, underlying biomechanism still largely unknown. In present study, we verify that GroEL can trigger activation NLRP3 inflammasome its downstream effector molecules, IL-1β IL-18, human ligament stem cells (hPDLSCs) resultantly induce high gelatinases (MMP-2 MMP-9) promote degradation extracellular matrix (ECM). GroEL-mediated requires participation Toll-like receptors (TLR2 TLR4). High upregulation TLR2 TLR4 induces enhancement NF-κB (p-p65) signaling promotes nuclear accumulation, thus activating inflammasome. These results are verified rat model with direct injection GroEL. Collectively, this study provides insight into bacteria-induced immune response may also provide new clue for prevention periodontitis.

Language: Английский

Citations

2
Histone demethylase KDM3B mediates matrix stiffness-induced osteogenic differentiation of adipose-derived stem cells DOI

Huangshui Ma,

Tao Zhang

Archives of Biochemistry and Biophysics, Journal Year: 2024, Volume and Issue: 757, P. 110028 - 110028

Published: May 18, 2024

Language: Английский

Citations

2
TGF‐β3 mediates mitochondrial dynamics through the p‐Smad3/AMPK pathway DOI Creative Commons

Xinmei Du,

Mengmeng Duan, Shiyi Kan

et al.

Cell Proliferation, Journal Year: 2023, Volume and Issue: 57(5)

Published: Nov. 27, 2023

Abstract It is well recognized that mitochondrial dynamics plays a vital role in cartilage physiology. Any perturbation could cause disorders metabolism and even lead to the occurrence of diseases such as osteoarthritis (OA). TGF‐β3, an important growth factor appears joints OA disease, shows its great potential chondrocyte metabolism. Nevertheless, TGF‐β3 on still not understood. Here we aimed investigate effect chondrocytes reveal underlying bio‐mechanism. By using transmission electron microscopy (TEM) for number morphology mitochondria, western blotting protein expressions, immunofluorescence cytoplasmic distributions proteins, RNA sequencing transcriptome changes related dynamics. We found increase mitochondria chondrocytes. TGF‐β3‐enhanced was via promoting fission. The fission induced by mediated AMPK signaling. activated canonical p‐Smad3 signaling resultantly AMPK‐induced Taken together, these results elucidate understanding provide cues therapeutic strategies injury disease terms energy

Language: Английский

Citations

6
Theoretical optimization of micropillar arrays for structurally stable bioinspired dry adhesives DOI Creative Commons
Ke Ni, Zhengzhi Wang

Theoretical and Applied Mechanics Letters, Journal Year: 2024, Volume and Issue: 14(2), P. 100512 - 100512

Published: March 1, 2024

Inspired by the excellent adhesion performances of setae structure from organisms, micro/nano-pillar array has become one paradigms for adhesive surfaces. The micropillar arrays are composed resin pillars and substrate with different elastic modulus supporting. stress singularity at bi-material corner between can induce failure micropillar-substrate further hinder fabrication application arrays, yet design stability lacks systematical quantitative guides. In this work, we develop a semi-analytical method to provide full expressions distribution within combining analytical derivations numerical calculations. predictions field be obtained based on stress. good agreement FEM results demonstrates high reliability our method. By adopting strain energy density factor approach, pillar-substrate is assessed predicting corner. For mismatch pillar given in paper, improved increasing ratio shear that micropillar. Our study provides accurate guide optimization material combinations more stable bioinspired dry adhesives.

Language: Английский

Citations

1