Published: Jan. 1, 2024
Language: Английский
Nucleic Acids Research, Journal Year: 2025, Volume and Issue: 53(1)
Published: Jan. 7, 2025
Abstract Non-canonical nucleic acid structures play significant roles in cellular processes through selective interactions with proteins. While both natural and artificial G-quadruplexes have been extensively studied, the functions of i-motifs remain less understood. This study investigates aptamer BV42, which binds strongly to influenza A virus hemagglutinin unexpectedly retains its i-motif structure even at neutral pH. However, BV42 conformational heterogeneity hinders detailed structural analysis. Molecular dynamics simulations chemical modifications helped us identify a potential binding site, allowing for redesign eliminate diversity while retaining affinity. Nuclear magnetic resonance spectroscopy confirmed i-motif/duplex junction three-cytosine loop nearby. highlights unique features functional role molecular recognition target.
Language: Английский
Citations
3
Nature Nanotechnology, Journal Year: 2024, Volume and Issue: 19(12), P. 1849 - 1857
Published: Sept. 9, 2024
Language: Английский
Citations
6
Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1295, P. 342329 - 342329
Published: Feb. 2, 2024
Language: Английский
Citations
5
International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 267, P. 131401 - 131401
Published: April 4, 2024
Language: Английский
Citations
4
Biomaterials, Journal Year: 2024, Volume and Issue: 314, P. 122897 - 122897
Published: Oct. 17, 2024
Language: Английский
Citations
4
Small Methods, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 22, 2025
Abstract Current tumor vaccines suffer from inadequate immune responsive due to the insufficient release of antigens, low infiltration, and immunosuppressive microenvironment. DNA nanostructures with their ability precisely engineer, controlled release, biocompatibility, capability augment immunogenicity microenvironment, have gained significant attention for potential revolutionize vaccine designing. This review summarizes various applications in construction situ cancer vaccines, which can generate tumor‐associated antigens directly damaged tumors immune‐stimulation. The mechanisms components are listed, specific strategies constructing using explored underlying action elucidated. immunogenic cell death (ICD) induced by chemotherapeutic agents, photothermal therapy (PTT), photodynamic (PDT), radiation (RT) related building systematically summarized. different immunotherapy elaborated, exerts precise, long‐lasting, robust responses. current challenges future prospectives proposed. provides a holistic understanding evolving role development.
Language: Английский
Citations
0
Biomaterials Science, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
A facile approach for enhanced combination therapy using a low pH and NIR irradiation-dual sensitive nanogel.
Language: Английский
Citations
0
International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143555 - 143555
Published: April 1, 2025
Language: Английский
Citations
0
ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(22), P. 29235 - 29247
Published: May 21, 2024
Expanding the functions and applications of DNA by integrating noncanonical bases structures into biopolymers is a continuous scientific effort. An adenine-rich strand (A-strand) introduced as functional scaffold revealing, in presence low-molecular-weight cofactor cyanuric acid (CA, pKa 6.9), supramolecular hydrogel-forming efficacies demonstrating multiple pH-responsiveness. At pH 1.2, A-strand transforms parallel A-motif duplex hydrogel cross-linked AH+-H+A units due to protonation adenine (pKa 3.5). 5.2, coadded CA, helicene-like configuration formed between protonated generating A-CA triplex hydrogel. 8.0, undergoes transition liquid state deprotonation CA disassembly its constituent components. Density theory calculations molecular dynamics simulations, supporting structural reconfigurations are performed. The sequential pH-stimulated states rheometrically characterized. framework loaded with fluorescein-labeled insulin, release insulin from across barriers present gastrointestinal tract demonstrated. results provide principles for future application oral administration diabetes.
Language: Английский
Citations
3
Materials Today Bio, Journal Year: 2024, Volume and Issue: 27, P. 101136 - 101136
Published: June 22, 2024
Tumor mRNA vaccines present a personalized approach in cancer immunotherapy, encoding distinct tumor antigens to evoke robust immune responses and offering the potential against emerging variants. Despite this, clinical advancement of has been hampered by their limited delivery capacity inefficient activation antigen-presenting cells (APCs). Herein, we employed microfluidics technology engineer mannose-modified lipid-based nanovaccines for specifically targeting APCs. The encapsulation process efficiently entrapped cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) agonist along with antigens. targeted (TNVs) exhibited narrow particle size distribution, ensuring consistent efficient delivery. These TNVs significantly enhanced gene expression mRNA, facilitating antigen presentation activation. When compared non-targeted nanovaccines, outperformed terms Furthermore, combination anti-PD-L1 antibodies elicited synergistic anti-tumor effect. This was attributed antibodies' ability overcome suppression cells. Our findings suggest that treatment most memory results indicate integrating checkpoint inhibitors or other immunostimulatory agents may be crucial enhancing response.
Language: Английский
Citations
3